[Histonet] Need better results from older archival slides?

2012-11-19 Thread Rob Day

Hello,

Having problems getting good Immuno staining and in-situ hybridization results 
with older slides?

I am writing to see if histonet can suggest any scientists who may be 
interested in helping us with a study related to a  slide rejuvenation 
process we are developing.

We think  we have found a way to significantly improve the antigenicity of 
proteins in older archival FFPE preserved slides. Initial tests seem to 
indicate that our process can allow slides of 5-10 years age, possibly older, 
to respond to IHC analysis as if they were cut that day. The process may have 
additional uses and benefits too, including better preservation of cut 
sections, enhanced ISH results, stabilization of labile proteins and 
standardization / optimization of IHC staining protocols. We are excited about 
this development, but we are seeking some independent labs to help us verify 
our findings.

Please feel free to call me to discuss, or pass on this email to any 
investigators you may know of who are exploring ways to make better use of 
older archival slides.

Folio Biosciences is a provider of biosamples and a commercial research lab 
based in Columbus Ohio. See: www.foliobio.com.

Regards,

Rob Day.






Rob Day
Business Development 
Folio Biosciences
1476 Manning Pkwy, Powell, Ohio 43065
Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 |  Fax: (877) 
591-1815
http://foliobio.com

www.linkedin.com/in/robdaybiotech

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RE: [Histonet] Need better results from older archival slides?

2012-11-19 Thread Helen Fedor
Agreed!


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, November 19, 2012 11:16 AM
To: Rob Day; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Need better results from older archival slides?

Rob:
If you are really seeking for a confirming study, publish your data and 
probably there will be a confirmation study.
 
I went to the website you indicated and it seems to me that your original 
posting is just a subtle and delicate advertisement.
 
Just my opinion otherwise, why don't you tell us all what is that you do to 
obtain your rejuvenating results? 
René J.



From: Rob Day r...@foliobio.com
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, November 19, 2012 11:03 AM
Subject: [Histonet] Need better results from older archival slides?


Hello,

Having problems getting good Immuno staining and in-situ hybridization results 
with older slides?

I am writing to see if histonet can suggest any scientists who may be 
interested in helping us with a study related to a  slide rejuvenation 
process we are developing.

We think  we have found a way to significantly improve the antigenicity of 
proteins in older archival FFPE preserved slides. Initial tests seem to 
indicate that our process can allow slides of 5-10 years age, possibly older, 
to respond to IHC analysis as if they were cut that day. The process may have 
additional uses and benefits too, including better preservation of cut 
sections, enhanced ISH results, stabilization of labile proteins and 
standardization / optimization of IHC staining protocols. We are excited about 
this development, but we are seeking some independent labs to help us verify 
our findings.

Please feel free to call me to discuss, or pass on this email to any 
investigators you may know of who are exploring ways to make better use of 
older archival slides.

Folio Biosciences is a provider of biosamples and a commercial research lab 
based in Columbus Ohio. See: http://www.foliobio.com/.

Regards,

Rob Day.






Rob Day
Business Development 
Folio Biosciences
1476 Manning Pkwy, Powell, Ohio 43065
Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 |  Fax: (877) 
591-1815
http://foliobio.com

www.linkedin.com/in/robdaybiotech

This message, including any attachments, is confidential and may be privileged 
or may contain health information protected by state and federal law.
Information and opinions expressed in this message and/or attachments are those 
of the author and are not necessarily those of the company. 
If you are not the intended recipient, please notify the sender and delete this 
message from your system. 
Any use of this information by individuals other than the intended recipient is 
strictly prohibited. 




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Re: [Histonet] Need better results from older archival slides?

2012-11-19 Thread Rene J Buesa
Rob:
If you are really seeking for a confirming study, publish your data and 
probably there will be a confirmation study.
 
I went to the website you indicated and it seems to me that your original 
posting is just a subtle and delicate advertisement.
 
Just my opinion otherwise, why don't you tell us all what is that you do to 
obtain your rejuvenating results? 
René J.



From: Rob Day r...@foliobio.com
To: histonet@lists.utsouthwestern.edu 
Sent: Monday, November 19, 2012 11:03 AM
Subject: [Histonet] Need better results from older archival slides?


Hello,

Having problems getting good Immuno staining and in-situ hybridization results 
with older slides?

I am writing to see if histonet can suggest any scientists who may be 
interested in helping us with a study related to a  slide rejuvenation 
process we are developing.

We think  we have found a way to significantly improve the antigenicity of 
proteins in older archival FFPE preserved slides. Initial tests seem to 
indicate that our process can allow slides of 5-10 years age, possibly older, 
to respond to IHC analysis as if they were cut that day. The process may have 
additional uses and benefits too, including better preservation of cut 
sections, enhanced ISH results, stabilization of labile proteins and 
standardization / optimization of IHC staining protocols. We are excited about 
this development, but we are seeking some independent labs to help us verify 
our findings.

Please feel free to call me to discuss, or pass on this email to any 
investigators you may know of who are exploring ways to make better use of 
older archival slides.

Folio Biosciences is a provider of biosamples and a commercial research lab 
based in Columbus Ohio. See: http://www.foliobio.com/.

Regards,

Rob Day.






Rob Day
Business Development 
Folio Biosciences
1476 Manning Pkwy, Powell, Ohio 43065
Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 |  Fax: (877) 
591-1815
http://foliobio.com

www.linkedin.com/in/robdaybiotech

This message, including any attachments, is confidential and may be privileged 
or may contain health information protected by state and federal law.
Information and opinions expressed in this message and/or attachments are those 
of the author and are not necessarily those of the company. 
If you are not the intended recipient, please notify the sender and delete this 
message from your system. 
Any use of this information by individuals other than the intended recipient is 
strictly prohibited. 




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[Histonet] 88305TC starting to hit the fan...

2012-11-19 Thread Webster, Thomas S.
CAP had a webinar last week about the cut. These are some very scary times. For 
some reason the government has decided to shift wealth from specialists to 
family practice. I am becoming more angry with the affordable care act everyday.

http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html_state=maximized_pageLabel=cntvwr


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Columbus Regional Hospital
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Re: [Histonet] 88305TC starting to hit the fan...

2012-11-19 Thread Jay Lundgren
Because there is a huge shortage of primary care docs as it is, which will
only get worse when millions of uninsured people suddenly have insurance.
 So I guess they are trying to entice more med students into primary care.
 There is a push to train more physician's assistants too

As it is, the top medical students want to go into something like radiology
or dermatology so they can work banker's hours and make 5x as much money as
a PCHP, and who can blame them?

I friend of mine who is a radiology tech saw a locum radiologist's pay stub
left on the fax machine (by accident), and said radiologist makes $280./hr.

  Jay A. Lundgren, M.S., HTL (ASCP)


On Mon, Nov 19, 2012 at 3:25 PM, Webster, Thomas S. twebs...@crh.orgwrote:

 CAP had a webinar last week about the cut. These are some very scary
 times. For some reason the government has decided to shift wealth from
 specialists to family practice. I am becoming more angry with the
 affordable care act everyday.


 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html_state=maximized_pageLabel=cntvwr


 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use of the
 intended recipient(s) and may contain confidential and privileged
 information. You may NOT use, disclose, copy or disseminate this
 information.  If you are not the intended recipient, please contact the
 sender by reply e-mail immediately.  Please destroy all copies of the
 original message and all attachments. Your cooperation is greatly
 appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 47201___
 Histonet mailing list
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 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] 88305

2012-11-19 Thread Bruce Gapinski
I am in the middle of the biggest cost study because we need to reevaluate the 
cost of our work. We have no expectation of keeping any derm clients after the 
change in the 88305. They will go elsewhere to get a slightly better price than 
here in California.
This is depressing on many levels.

1.   Lay-offs

2.   Inferior quality (we are VERY proud of our work)

3.   TAT nightmares.

4.   Where does that leave our true client ( THE PATIENT)?
Furthermore I may be out of a job (last 35 years) and the pathologists may farm 
out our surgical work too.  How does this impact your Histology laboratory?
Respectfully,
Bruce Gapinski


Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you
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Re: [Histonet] 88305TC starting to hit the fan...

2012-11-19 Thread Davide Costanzo
While this stinks on many levels, I have to take issue with the shift
wealth from specialists to family practice - family practice docs
have been the frontline of medicine, all the while earning less than a
quarter of what specialists earn. It's about time they get a boost.
Too many specialists earn over a million a year, while the family
practice guys/ladies can barely pay their student loans.

Sent from my iPhone

On Nov 19, 2012, at 1:25 PM, Webster, Thomas S. twebs...@crh.org wrote:

 CAP had a webinar last week about the cut. These are some very scary times. 
 For some reason the government has decided to shift wealth from specialists 
 to family practice. I am becoming more angry with the affordable care act 
 everyday.

 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html_state=maximized_pageLabel=cntvwr


 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use of the
 intended recipient(s) and may contain confidential and privileged
 information. You may NOT use, disclose, copy or disseminate this
 information.  If you are not the intended recipient, please contact the
 sender by reply e-mail immediately.  Please destroy all copies of the
 original message and all attachments. Your cooperation is greatly
 appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 47201___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] 88305TC starting to hit the fan...

2012-11-19 Thread Paula Sicurello
Sure they want more physicians.  My husband was forced to leave
medical school when he became sick (they wouldn't give him a medical
leave of absence).  No other medical school would touch him after
that.  They thought something was fishy, his transcript reads medical
leave of absence which wasn't true.  The other shcools can't figure
out why the school he was in wouldn't take him back.  If he wanted to
retake the MCAT and apply cold, they told him he could do that.

Medicine is a self limiting profession.  The AMA determines how many
doctors are availabe to keep a limited resource, limited. Thus the
$280/hour radiologist.

You leave with close to $200,000 in debt if attending a private
school.  Primary care pays, in most regions, under $100,000/yr.  When
you have that kind of debt, lower paying primary care jobs aren't
enticing.

My bitter two cents.

Paula

On Mon, Nov 19, 2012 at 4:25 PM, Webster, Thomas S. twebs...@crh.org wrote:
 CAP had a webinar last week about the cut. These are some very scary times. 
 For some reason the government has decided to shift wealth from specialists 
 to family practice. I am becoming more angry with the affordable care act 
 everyday.

 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html_state=maximized_pageLabel=cntvwr


 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use of the
 intended recipient(s) and may contain confidential and privileged
 information. You may NOT use, disclose, copy or disseminate this
 information.  If you are not the intended recipient, please contact the
 sender by reply e-mail immediately.  Please destroy all copies of the
 original message and all attachments. Your cooperation is greatly
 appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 47201___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] Need parts for old equipment

2012-11-19 Thread Cheryl Crowder
Hello all - 


I have been volunteering in a lab with very old equipment.  Although I was 
able to find 2 processors, they have both died.  We do not have the money 
to buy even a used processor, so I am looking for refurbished parts.  I am 
hoping there are some vendors out there who have parts that we need or if 
there is a lab with a processor that works that they don't want we might be 
able to pay shipping.  

  

I have a VIP 3000 and need a motor, gear head, bearing washer and roller 
washer.  The other processor is a Tissue-Tek II (c. 1983).  I think I need a 
optoelectronic switch.  

   

  Any help any one of you can give me, contact me personally.  Thank you in 
advance,


Cheryl Crowder, BA, HTL(ASCP)

Animal Science Department

LSU

Baton Rouge, LA  70820

(225)  772-2865
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Re: [Histonet] 88305TC starting to hit the fan...

2012-11-19 Thread David Kemler
Hmmm...I think more people should have paid attention to Obamacare two years 
ago when it was being shoved down eveyones throat. Oh well.. as old saying 
goes...You ain't seen nottin' yet! I'm just amazed that there are so many in 
the profession who are surprised about the changes beginning to take place, 
there are many, many more to come in 2013  14 and all of them affect your job 
or lack thereof. :)
 
Everyone had the opportunity to change things on November 6th - they chose not 
to. So, you live with it.
 
Yours,
David
 
 



From: Davide Costanzo pathloc...@gmail.com
To: Webster, Thomas S. twebs...@crh.org 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Monday, November 19, 2012 6:46 PM
Subject: Re: [Histonet] 88305TC starting to hit the fan...

While this stinks on many levels, I have to take issue with the shift
wealth from specialists to family practice - family practice docs
have been the frontline of medicine, all the while earning less than a
quarter of what specialists earn. It's about time they get a boost.
Too many specialists earn over a million a year, while the family
practice guys/ladies can barely pay their student loans.

Sent from my iPhone

On Nov 19, 2012, at 1:25 PM, Webster, Thomas S. twebs...@crh.org wrote:

 CAP had a webinar last week about the cut. These are some very scary times. 
 For some reason the government has decided to shift wealth from specialists 
 to family practice. I am becoming more angry with the affordable care act 
 everyday.

 http://www.cap.org/apps/cap.portal?_nfpb=truecntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow_windowLabel=cntvwrPtltcntvwrPtlt%7BactionForm.contentReference%7D=advocacy%2Fadvocacy_related_webinars.html_state=maximized_pageLabel=cntvwr


 CONFIDENTIALITY NOTICE:
 This e-mail message, including all attachments, is for the sole use of the
 intended recipient(s) and may contain confidential and privileged
 information. You may NOT use, disclose, copy or disseminate this
 information.  If you are not the intended recipient, please contact the
 sender by reply e-mail immediately.  Please destroy all copies of the
 original message and all attachments. Your cooperation is greatly
 appreciated.
 Columbus Regional Hospital
 2400 East 17th Street
 Columbus, Indiana 47201___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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