[Histonet] Ultra users
Has anyone encountered incorrect/inappropriate staining using the Ultra? Twice we have had slides that looked like they had the wrong antibody applied, although the slides and dispensers were labeled correctly (once with TTF-1, once with p16; both had membranous staining). I was wondering if where the reagent wheel both spins and moves on the arm, occasionally it doesn't quite make it to the dispenser it is supposed to and applies the wrong one. Does anyone have any experience/ideas as to why this might happen? thank you! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GMS fungus control
Second question of the day: what is everyone using for their fungus control for GMS? We have always used pneumocystis when looking for pneumo, and Aspergillus when looking for fungus, but wondering if anyone uses any type of fungus just to see that stain worked properly. We are having difficulty locating tissue for both pneumo and Aspergillus, and are trying to avoid buying commercial QC slides. thanks again! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Ultra users
Clare, last month we had recurring troubles with the Ultra. With a couple of runs the slides were not stained correctly. In one run we had slides with no colour, with dab-colour, with purple-hematoxylin color, with specific antibody-staining and without. The whole panorama. Some runs were completed without any alarm. Some stopped with the alarm that's due to the fact, that the arm didn't find the right position at the end of its turn. This error was told to be caused by an invalid sensor, that is humpered by too much moisture (fog) in the ultra. Ventana said the slides are the culprit (like every time). We took they recommended from Leica. And had the same troubles. After that no one really had an idea. Now we've got a new Ultra. Tomorrow I know more, if it was the instrument or the reagens. Our Ultra is now about two years old and has been running quite 24 hours a day I also have the suspiscion, that the spinning arm and the hammer are the cause. Please tell me more about your troubles. And how you fixed it. Kind regards Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Clare Thornton Gesendet: Montag, 26. November 2012 15:42 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Ultra users Has anyone encountered incorrect/inappropriate staining using the Ultra? Twice we have had slides that looked like they had the wrong antibody applied, although the slides and dispensers were labeled correctly (once with TTF-1, once with p16; both had membranous staining). I was wondering if where the reagent wheel both spins and moves on the arm, occasionally it doesn't quite make it to the dispenser it is supposed to and applies the wrong one. Does anyone have any experience/ideas as to why this might happen? thank you! Clare J. Thornton, HTL(ASCP)QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Wrinkle Out
Good morning! Is anybody using this product, Wrinkle Out Water Bath H@O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. Thanks! Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 88305TC starting to hit the fan...
Mr. Buesa . . .You're always a voice of reason. Thank you!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Wrinkle Out
Avoid all those dangers and just add any liquid soap at 0.5% in your water bath with the same effects René J. From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: HISTONET histonet@lists.utsouthwestern.edu Sent: Monday, November 26, 2012 10:47 AM Subject: [Histonet] Wrinkle Out Good morning! Is anybody using this product, Wrinkle Out Water Bath H@O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. Thanks! Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation?
Hello all, We were using (with great success) the Max Vision Max Poly-Two with our mostly DAKO antibodies on our Lab Vision autostainer. Mostly canine and feline tissues, mostly tumor markers. We were so happy. Better quality, great specificity, great reproducibility, and half the price of the Lab Vision polymer detection kit. Now, Max Vision is discontinuing their IHC line, and we are filled with woe and wringing our collective histology hands. Any recommendations for a quality rabbit/mouse polymer detection kit would be most appreciated. Thank you, Sandy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Wrinkle Out
Rene, Does the soap affect IHC or ISH stains? Amanda On Mon, Nov 26, 2012 at 12:13 PM, Rene J Buesa rjbu...@yahoo.com wrote: Avoid all those dangers and just add any liquid soap at 0.5% in your water bath with the same effects René J. From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu To: HISTONET histonet@lists.utsouthwestern.edu Sent: Monday, November 26, 2012 10:47 AM Subject: [Histonet] Wrinkle Out Good morning! Is anybody using this product, Wrinkle Out Water Bath H@O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. Thanks! Andi Grantham Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Amanda Kelley Histology Supervisor St. Louis University Medical School Department of Pathology 1402 S. Grand Blvd. St. Louis Mo. 63104 Phone: (314) 977-7868 Fax: (314) 977-8740 akell...@slu.edu DISCLAIMER: The contents of this e-mail, including any attachments, contain information which may be confidential, legally privileged, proprietary in nature, or otherwise protected by law from disclosure, and is solely for the use of the intended recipient(s). If you are not the intended recipient, any use, disclosure or copying of this e-mail, including any attachments, is unauthorized and strictly prohibited. If you have received this e-mail in error, please notify us via return e-mail and immediately delete all copies of it from your system. Any opinions either expressed or implied in this e-mail and all attachments, are those of its author only, and do not necessarily reflect those of Saint Louis University. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Wrinkle Out Water Bath
You wrote: Is anybody using this product, Wrinkle Out Water Bath H http://lists.utsouthwestern.edu/mailman/listinfo/histonet @t O, in their waterbaths and what precautions are you taking if so? The label states that the product is highly toxic, a hepatoxin. I received a sample of it and wasn't aware of its toxicity before I requested the sample at the NSH S/C. In reading the MSDS for this product, all it contains is 1% isopropyl, 18% Ethanol, and 1% methanol citing possible damage to liver. Why not avoid spending the money and just use 20% ethanol in your water bath according to how this product is used.It will work just as well as this.You could even make up your own mixture with isopropyl and ethanol, forget the more toxic and expensive methanol. These alcohols are all used for tissue processing e.g. denatured reagent alcohols, isopropyl, ethanol and/or methanol alone, and just as toxic to the liver. Beware! Too high of an alcohol concentration can cause your sections to explode on a warm water bath. We never used alcohol in warm water bath to flatten tissue sections. Instead, we used RT 10% ethyl alcohol in a glass staining dish, floated a section on this, picked up section onto a slide then went to a warm water bath, gently lowered the section but kept top paraffin portion of section on the slide, and watch the section flatten in the warm water. This prevented a section from exploding wildly or losing the section on the water bath. All the alcohol or a detergent does is reduce the surface tension of the water so the section flattens. Tween 20 has been used too. I don't think any of these damage IHC since the antigens are still protected by the paraffin in the tissue section. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabbit/Mouse Polymer Detection Kit Recommendation?
Biocare and Golden Bridge International have kits.I believe Vector can be added to this group too. Gayle Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: GMS fungus control
Clare J. Thornton, HTL(ASCP)QIHC at Dahl-Chase Diagnostic Services, Bangor, Maine asks: What is everyone using for their fungus control for GMS? We have always used pneumocystis when looking for pneumocystis, and Aspergillus when looking for fungus, but wondering if anyone uses any type of fungus just to see that stain worked properly. We are having difficulty locating tissue for both pneumo and Aspergillus, and are trying to avoid buying commercial QC slides. It's preferable to have a pneumocystis control when needed. Histoplasma is the best fungus control, when you can get it, because it's the most difficult to stain. In ordinary surgical material, aspergillus is most often seen in paranasal sinus material. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ultra
I too have had problems with my Ultra. I am so glad I place a control at the top of every slide. Sometimes no Hematoxylin, sometimes no positive staining. I've documented half a dozen cases. One thing we noticed is that the antibody vials get plugged up from the protein in the antibody. We inspect every vial before we place it on the instrument. The other thing we look for is antibody in the spout of the vial. It will recede. Called Ventana, and they said There are plenty of extra drops in the vial, so prime the vial. Bad advise, here's why. The instrument has no idea how many drops are disposed of during priming. So we ended up with another bunch of primaries with no drops left. Now we prime without expelling any reagent and how that works better. With this problem I feel Ventana owes us URA (Ultimate Reagent Access) then we can prime as we go. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ultra
We have the same issue from time to time on our Ultra, our vortex mixers seem to clog after about 3-4 months of use. The engineer comes in, unclogs them and everything is good for another 3-4 months. They have never really been able to figure it out why they clog but, but thankfully the service is still covered, just a large nuisance. Daniel Hewitt Histology Supervisor, HVS 412-749-7371 This email, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, or an agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and delete and destroy all copies of the original message, including attachments. Please note that any views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of Heritage Valley Health System. The integrity and security of this message cannot be guaranteed on the internet. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bruce Gapinski Sent: Monday, November 26, 2012 1:50 PM To: 'cthorn...@dahlchase.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ultra I too have had problems with my Ultra. I am so glad I place a control at the top of every slide. Sometimes no Hematoxylin, sometimes no positive staining. I've documented half a dozen cases. One thing we noticed is that the antibody vials get plugged up from the protein in the antibody. We inspect every vial before we place it on the instrument. The other thing we look for is antibody in the spout of the vial. It will recede. Called Ventana, and they said There are plenty of extra drops in the vial, so prime the vial. Bad advise, here's why. The instrument has no idea how many drops are disposed of during priming. So we ended up with another bunch of primaries with no drops left. Now we prime without expelling any reagent and how that works better. With this problem I feel Ventana owes us URA (Ultimate Reagent Access) then we can prime as we go. Bruce Gapinsk HT (ASCP) Chief Histologist Marin Medical Laboratories PathGroup SF Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
