RE: [Histonet] Space requirements for AP lab
We are Joint Commission. I've never run across this issue (and God forbid bringing it up in front of one!) in 40+ years and 3 lab renovations. CAP people out there?? Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Wednesday, December 12, 2012 4:43 PM To: jmasla...@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things. Kindness is the language the blind can see the deaf can hear- Mark Twain ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Her2 Dual ISH and breast processing
I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying to convince our pathologists about the importance of good fixation, but the pathologist are always in a hurry to get the diagnosis out. Many mamma biopsies do not get to be fixed long enough (some are even put in the processor after a few hours of fixation). It would be helpfull if I can show them some literature to back me up. Willem Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtol...@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental step to solving your staining problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). René J. From: vtol...@cox.net vtol...@cox.net To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened. We are now getting breast sections that are consistently cut between 2-3mm in thickness. However, we are still having issues with inconsistency in our dual ISH staining. Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor. Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Space requirements for AP lab
Years ago I worked in a small, community hospital. The histology lab was TINY! very well organized and tidy...but TINY! Every inspection we got cited for being too small. Can't remember which agency but we were a teaching hospital. Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 13, 2012 7:05 AM To: Morken, Timothy; jmasla...@stpetes.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab We are Joint Commission. I've never run across this issue (and God forbid bringing it up in front of one!) in 40+ years and 3 lab renovations. CAP people out there?? Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Wednesday, December 12, 2012 4:43 PM To: jmasla...@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things. Kindness is the language the blind can see the deaf can hear- Mark Twain ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP6 Questions
Good morning Everyone: For those of you who have a Sakura VIP6, do you actually use the bulk reservoirs to let the machine automatically rotate the absolute alcohols and xylene? If not, why not? Also do you let the machine automatically rotate the paraffin reservoirs, or do you do this manually, and if so, why? Also, do you find the touch screen graphics easy to use? Have any problems developed with the display itself? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP6 issue
Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Path Lab Assistant Job
Northeast Philadelphia physician in office path laboratory is looking for a laboratory assistant. Must have strong computer skills. Duties include data entry, phones, inventory control, filing of slides, and miscellaneous tasks. 15 to 20 hours per week. Please fax resume to 215 947-2015 attention Laboratory Manager. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Synoptic Reporting
What are people doing for synoptic reporting? Are the CAP templates the only option? ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] VIP6 issue
I've always used a lid on the basket when using the VIP. If we did not do so, the blocks will float around, but we have not experienced cassette lids coming off. Is the lid or rack something in addition to a lid being on the basket already? Brendal Finlay, HT (ASCP) Medical Center Clinic brendal.fin...@medicalcenterclinic.com 850.474.8758 http://medicalcenterclinic.com -Original message- From: Nancy Schmitt nancy_schm...@pa-ucl.com Date: Thu, 13 Dec 2012 09:17:24 -0600 To: Histonet (histonet@lists.utsouthwestern.edu)histonet@lists.utsouthwestern.edu Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lidon top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Her2 Dual ISH and breast processing
My mistake: the web site is: http://www.histosearch.com/rene.html From: Rene J Buesa rjbu...@yahoo.com To: Hoekert, W.E.J. w.e.j.hoek...@olvg.nl; vtol...@cox.net vtol...@cox.net; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, December 13, 2012 10:47 AM Subject: Re: [Histonet] Her2 Dual ISH and breast processing Please go to http://www.histosearch.co/rene.html There are 2 articles on the subject: one about the general fixation issue and another about the minimum amount of NBF required to obtain complete fixation. René J. From: Hoekert, W.E.J. w.e.j.hoek...@olvg.nl To: Rene J Buesa rjbu...@yahoo.com; vtol...@cox.net; histonet@lists.utsouthwestern.edu Sent: Thursday, December 13, 2012 7:35 AM Subject: RE: [Histonet] Her2 Dual ISH and breast processing I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying to convince our pathologists about the importance of good fixation, but the pathologist are always in a hurry to get the diagnosis out. Many mamma biopsies do not get to be fixed long enough (some are even put in the processor after a few hours of fixation). It would be helpfull if I can show them some literature to back me up. Willem Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtol...@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental step to solving your staining problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). René J. From: vtol...@cox.net vtol...@cox.net To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened. We are now getting breast sections that are consistently cut between 2-3mm in thickness. However, we are still having issues with inconsistency in our dual ISH staining. Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor. Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Her2 Dual ISH and breast processing
Please go to http://www.histosearch.co/rene.html There are 2 articles on the subject: one about the general fixation issue and another about the minimum amount of NBF required to obtain complete fixation. René J. From: Hoekert, W.E.J. w.e.j.hoek...@olvg.nl To: Rene J Buesa rjbu...@yahoo.com; vtol...@cox.net; histonet@lists.utsouthwestern.edu Sent: Thursday, December 13, 2012 7:35 AM Subject: RE: [Histonet] Her2 Dual ISH and breast processing I was wondering,is there any literature on this subject? i.e. the minimal required fixation time of breast tissue in order to get reliable immuno staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying to convince our pathologists about the importance of good fixation, but the pathologist are always in a hurry to get the diagnosis out. Many mamma biopsies do not get to be fixed long enough (some are even put in the processor after a few hours of fixation). It would be helpfull if I can show them some literature to back me up. Willem Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa Verzonden: ma 10-12-2012 18:23 Aan: vtol...@cox.net; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] Her2 Dual ISH and breast processing Val: Acknowledging that you have a fixation problem is the fundamental step to solving your staining problems. You state that the slices have now a consistent thickness of between 2-3 mm and that is great BUT what about the fixation time? Thin slices is a first step but until you have the slices properly fixed, you will keep having some problems. Even when one should never assume, I assume that you are using NBF at room temperature. With that fixative and under those temperature conditions, your 2-3 mm thick breast slices will require 4 hours to be fully penetrated; will require 24 hours to be 100% covalent bound and will require 96 hours to be completely cross-linked. The 48 hours of maximum exposition to NBF recommended by ASCO-CAP will guarantee a 100% cross-linking of 1 mm thick slices, but your 2-3 mm slices will require 96 hours to be completely cross-linked. Until you reach an optimum fixation you will keep having sporadic problems of your protocols (depending on greater or lower fat contents of the samples). René J. From: vtol...@cox.net vtol...@cox.net To: histonet@lists.utsouthwestern.edu Sent: Monday, December 10, 2012 11:48 AM Subject: [Histonet] Her2 Dual ISH and breast processing Hello all-- My lab is having some problems with inconstant results with our Her2 Dual ISH on the Ventana Ultra. Right now, we know we have a problem with our large breast tissue being under-fixed. There have been many gripes to the PAs and residents about the thickness of the tissue sections and they have listened. We are now getting breast sections that are consistently cut between 2-3mm in thickness. However, we are still having issues with inconsistency in our dual ISH staining. Many times, the staining is absent. We are currently processing our breast tissue on a Tissue-Tek VIP6 processor. Are there any labs out there that are using this processor and also running Her2 Dual ISH on the Ventana with nice, consistent results? If so, would any of you be willing to share your processing protocol? Thanks in advance for your help! Val ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP6 issue
Sorry for the confusion - the cassettes lids are not coming off. The lid for the rack which came with the new processor get off kilter during processing allowing the cassettes to float up out of the rack. Nancy NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Space requirements for AP lab
It's not a theoretical question. When I came here I found our EM lab / Muscle histochem Lab / IF lab are crammed into one small space. It had 30years of accumulated stuff that no one wanted to throw out. There were microscope tables in the middle of walkways, the histochem staining area is the primary path into the lab - and the walkway itself is inadequately sized - imagine crowding through when staining is going on! JC inspectors cited the lab for inadequate working space. That put focus on a lab that had been largely ignored and we spent a long while decluttering, surplussing unused equipment and getting a new, larger, fume hood. (Thank you JC!!) That said, I think it is a very subjective judgment by an inspector and I am not aware of any specific space requirements that any agency applies beyond adequate. In this case it was one inspector out of dozens that had been through the lab previously. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Lynette Pavelich [mailto:lpave...@hurleymc.com] Sent: Thursday, December 13, 2012 4:05 AM To: Morken, Timothy; jmasla...@stpetes.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab We are Joint Commission. I've never run across this issue (and God forbid bringing it up in front of one!) in 40+ years and 3 lab renovations. CAP people out there?? Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Wednesday, December 12, 2012 4:43 PM To: jmasla...@stpetes.org; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Space requirements for AP lab Administrators rule of thumb: If there is free space in Histology, they are taking up too much space! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org Sent: Monday, December 10, 2012 9:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Space requirements for AP lab Greetings All, Are there any JACHO or CAP space requirements for an AP laboratory? Looking for recommended square footage per tech, equipment, and/or work area. Thanks Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things. Kindness is the language the blind can see the deaf can hear- Mark Twain ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ubiquitin and alpha synuclein
What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: VIP6 issue
I talked with the rep. and they said they had never heard of this. Right. Yes, it happens. Often. Too much air caught in the cassettes. Worse with the fine-mesh cassettes. Bounce the racks in the formalin tray a few times before loading on the processor to try to get as much air out as possible. That will improve processing as well. And weight down the tops if it continues. Tim Morken Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 13, 2012 6:17 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ubiquitin and alpha synuclein
Jennifer, Thermo/Lab Vision has some excellent Synuclein antibodies. Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harvey, Jennifer Lynn Sent: Thursday, December 13, 2012 9:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ubiquitin and alpha synuclein What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ubiquitin and alpha synuclein
Jennifer, We use p62/SQSTM1 from MBL (Medical Biological Laboratories, Co., LTD), code M162-3, as our Ubiquitin and alpha Synuclein from Invitrogen, #18-0215. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harvey, Jennifer Lynn Sent: Thursday, December 13, 2012 11:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ubiquitin and alpha synuclein What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP6 questions
We do not use the bulk reservoirs at this time - our storeroom is 6 feet away. We do automatically rotate paraffin and love it! Yes - we find the touch screen easy to use. No problems have developed, but it is still very new. Would you share your opinions on these questions? Nancy Schmitt United Clinical Laboratories Dubuque, IA -- Message: 10 Date: Thu, 13 Dec 2012 09:05:19 -0500 From: Tim Wheelock twheel...@mclean.harvard.edu Subject: [Histonet] VIP6 Questions To: Histonet@lists.utsouthwestern.edu Message-ID: 50c9e09f.2060...@mclean.harvard.edu Content-Type: text/plain; charset=ISO-8859-1; format=flowed Good morning Everyone: For those of you who have a Sakura VIP6, do you actually use the bulk reservoirs to let the machine automatically rotate the absolute alcohols and xylene? If not, why not? Also do you let the machine automatically rotate the paraffin reservoirs, or do you do this manually, and if so, why? Also, do you find the touch screen graphics easy to use? Have any problems developed with the display itself? Thanks, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Maximum bone sample size for methyl methacrylate embedding
Dear Histonetters, Would anyone advise on the maximum size sample of undecalcified bone which could be properly processed into methyl methacrylate for sectioning and staining for Goldner's trichrome? Would anyone have a protocol for processing large bone samples (possibly 2 x1cm) into MMA as most of the protocols I've seen are based on Bordier trephine 5mm iliac crest biopsies. Thank you, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX Website: http://mellanbycentre.dept.shef.ac.uk Tel: 00353114-2713337 (office) 00353114-2713174 (lab) E-Mail:o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: VIP6 issue
One of the products I learned about at the 2012 NSH convention was ActivFlo tissue cassettes. Designed by JB McCormick (I think) they are biopsy cassettes that have side vents to help disperse the trapped air bubbles. Here is a link from Leica, they sell several different ActiveFlo cassettes... http://www.leicabiosystems.com/products/consumables/cassettes-base-molds/biopsy-cassettes/ Brett Brett M. Connolly, Ph.D. Principal Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Thursday, December 13, 2012 12:45 PM To: Morken, Timothy; Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: VIP6 issue Tim brings up a good point. I would monitor the processing in the fine mesh type cassettes as well. For years, and I do mean years, we struggled with our tiny specimens. Why did some specimens in mesh cassettes do just fine, some notit was an absolute nightmare! Monitored how the specimen was handled starting right at the sight of surgery on through changing processing schedules/times/heat?/no heat/xylene/xylene substitute/staining schedules. We wet the mesh cassettes prior to loading, then trying to swish the air pocket out of the cassette before processing. (did not experience the lids coming off) We found that the only thing that has produced consistently good results was when we went back to the lens paper. What we think was going on, was that the little biopsies would intermittently get caught up in a air bubble during processing (agitation, pumping in/out), thus missing being in that current solution(s). It truly was a nightmare.. We now purchase cheap lens paper, and cut it into 4 squares..paper cutter does a large amount quickly and we're good to go. Yes, it is a little more hassle unwrapping them in the morning, but if it gives a better quality specimen then it doesn't matter!! Just my two cents worth;) Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Thursday, December 13, 2012 12:03 PM To: Nancy Schmitt; Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] RE: VIP6 issue I talked with the rep. and they said they had never heard of this. Right. Yes, it happens. Often. Too much air caught in the cassettes. Worse with the fine-mesh cassettes. Bounce the racks in the formalin tray a few times before loading on the processor to try to get as much air out as possible. That will improve processing as well. And weight down the tops if it continues. Tim Morken Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, December 13, 2012 6:17 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] VIP6 issue Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates Direct contact information for affiliates is available at
[Histonet] Re: Histonet Digest, Vol 109, Issue 14
Val, Go to CAP website (http://www.cap.org/apps/cap.portal) and you will find the answer to your question, or give them a call @ 800-323-4040. HER2 have a very specific guide line for clinical test. My understanding the fixation should be minimun 6 hrs maximum 48 hrs. Your problem could be resolved if you have a good rapid tissue processor (ie. Leica - Peloris, etc). We have the same problem with VIP5 before I purchased the Peloris. I haven't have one single re-process since then. There is a trick to my problem. Give me an email if you want to know. Madeleine Huey, HTL QIHC (ASCP) madelein...@elcaminohospital.com On Thu, Dec 13, 2012 at 9:04 AM, histonet-requ...@lists.utsouthwestern.edu wrote: Her2 Dual ISH and breast processing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: VIP6 issue14
Nancy, Like you, we had that problem and had to put something on top to prevent the cassettes from floating around. Debbie Dreesen Message: 11 Date: Thu, 13 Dec 2012 14:17:24 + From: Nancy Schmitt nancy_schm...@pa-ucl.com Subject: [Histonet] VIP6 issue To: Histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Message-ID: 906b4da90ed1db4db6c7e94d7cee6c36220c1...@peitha.wad.pa-ucl.com Content-Type: text/plain; charset=us-ascii Good Morning- We have recently purchased VIP6 processors. Has anyone else experienced a problem with the lids coming off during pump in and pump out? Causes the cassettes to float all over and be completely out of order:( We now place an extra rack or lid on top to weigh down and insure this does not happen. I talked with the rep. and they said they had never heard of this. I know this is not a huge deal, but with new instrumentation I don't think we be cobbling things already. Thank you for any input- Nancy Schmitt Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
