[Histonet] RE: H Pylori Stain
If you are currently doing IHC, you might want to consider working up the H. Pylori with it. Our docs prefer it, as it is easier to identify them on the slide. Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Tasha Fraser [tfra...@olympicmedical.org] Sent: Thursday, January 03, 2013 2:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H Pylori Stain Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] H Pylori Stain
We use the HP Blue stain by Anatech. Katelin Lester, HTL On Jan 3, 2013 11:23 AM, Tasha Fraser tfra...@olympicmedical.org wrote: Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Helicobater Pylori QC block and/or slides
Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 110, Issue 4
Hi Tasha, Warthin-Starry will work very well. We used Dako Autostainer - Artisan in our lab, but manual will work as well. Good Luck! Supervisor - Pathology (IPOX Histology) Madeleine Huey BS, MT (CSMT), HTL QIHC (ASCP) madelein...@elcaminohospital.org Message: 4 Date: Thu, 3 Jan 2013 11:22:46 -0800 From: Tasha Fraser tfra...@olympicmedical.org Subject: [Histonet] H Pylori Stain To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 5290DE49B23B6240BC0DF14FC8142D1901CB907F@is-210vs.olympicmedical.local Content-Type: text/plain; charset=us-ascii Hi everyone! Does anyone have a good h-pylori stain? We currently use the Steiner and Steiner Stain. We've done some testing with the Alcian Yellow-Toluidine Blue Method, but not getting the same results as the book shows. Does anyone have any suggestions please. Tasha Fraser , HT (ASCP) Olympic Medical Center Port Angeles, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Helicobater Pylori QC block and/or slides
We use human precut control slides from Newcomer Supply. Katelin Lester, HTL On Jan 4, 2013 9:37 AM, Tasha Fraser tfra...@olympicmedical.org wrote: Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Helicobater Pylori QC block and/or slides
I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Tasha Fraser tfra...@olympicmedical.org 1/4/2013 12:37 PM Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Helicobater Pylori QC block and/or slides
Dr. Cartun is absolutely correct in always trying to use positive tissue that has been processed in your lab using the same process as your patient tissues. The control blocks offered through the Michigan society have been donated by various laboratories in the state, and have been tested prior to donation. Before mailing a control block, that block is cut and tested again, and the slide accompanies the block to the recipient. When I donate a block to the society to be used for IHC, I supply the information of what type of fixative and how long it remained in it prior to processing as this can be critical to IHC. With all due respect, these controls are only offered as an alternative to those who have trouble locating/budget constrictions. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard Cartun [rcar...@harthosp.org] Sent: Friday, January 04, 2013 2:20 PM To: Histonet@lists.utsouthwestern.edu; Tasha Fraser Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Tasha Fraser tfra...@olympicmedical.org 1/4/2013 12:37 PM Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Helicobater Pylori QC block and/or slides
Still a nice service, despite your points about its limitations. Many areas don't even have that option! It's a good thing for those who are having trouble. Sent from my Verizon Wireless 4G LTE Smartphone - Reply message - From: Lynette Pavelich lpave...@hurleymc.com To: Richard Cartun rcar...@harthosp.org, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, Tasha Fraser tfra...@olympicmedical.org Subject: [Histonet] Helicobater Pylori QC block and/or slides Date: Fri, Jan 4, 2013 1:47 pm Dr. Cartun is absolutely correct in always trying to use positive tissue that has been processed in your lab using the same process as your patient tissues. The control blocks offered through the Michigan society have been donated by various laboratories in the state, and have been tested prior to donation.. Before mailing a control block, that block is cut and tested again, and the slide accompanies the block to the recipient. When I donate a block to the society to be used for IHC, I supply the information of what type of fixative and how long it remained in it prior to processing as this can be critical to IHC. With all due respect, these controls are only offered as an alternative to those who have trouble locating/budget constrictions. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard Cartun [rcar...@harthosp.org] Sent: Friday, January 04, 2013 2:20 PM To: Histonet@lists.utsouthwestern.edu; Tasha Fraser Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides I use gastric resection tissue that is H. pylori positive. You can use biopsy tissue as well, but I would be careful to not cut-through the tissue in case retrospective testing is ordered. You should always try to use tissue that is fixed and processed in your own laboratory. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Tasha Fraser tfra...@olympicmedical.org 1/4/2013 12:37 PM Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PSCA Primary antibody
Hallo Histonetters, Happy New Year!! I'm looking for information on a PSCA (Prostate Specific Cell antigen) antibody that will work both in human and primate. If are are using this antibody, please contact me. Any information would be appreciated. thanks Dusko Trajkovic 858-638-6202 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Pathologists' Names on HE Labels
We don't have names on slides but I wish we did!! My first thought is that there would be no more liability on having their name on the slide than on the report. I don't understand the concern. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, January 04, 2013 5:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologists' Names on HE Labels For years, our lab's information system has been set up so that the name of the pathologist assigned to a case prints on our HE labels to assist with sorting and grouping the slides after staining. As new pathologists join our team, concerns regarding this practice have come up. The pathologists are concerned that having their names on the slides pose medical-legal liability, and they want the names removed. I've yet to hear clear ramifications involved with having doctors' names on slides. What liability is really involved? We are a large, urban academic medical center with high specimen volumes. Our pathologists rotate through different sub-specialties, sometimes on a daily basis. For us, having the pathologists' names on the HE labels seems to be the easiest way to get the HE's to the correct pathologist. Are other labs printing the names of the pathologists on their HE labels? Is anyone aware of any legal risk involved in this practice? Would anyone mind sharing how they sort and divide the slides before submitting them to their pathologists? Thank you, Roger Heyna, BS, HTL(ASCP) Loyola University Medical Center Maywood, IL This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Fwd: [Histonet] Helicobater Pylori QC block and/or slides
-- Forwarded message -- From: Hans B Snyder h...@histologistics.com Date: Fri, Jan 4, 2013 at 5:32 PM Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides To: Tasha Fraser tfra...@olympicmedical.org We have a human colon tissue that is positive. On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser tfra...@olympicmedical.orgwrote: Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Helicobater Pylori QC block and/or slides
We are a private histology lab that is willing to share. If you need slides or blocks let us know! thanks Sent from my iPad On Jan 4, 2013, at 8:06 PM, Hans B Snyder h...@histologistics.com wrote: -- Forwarded message -- From: Hans B Snyder h...@histologistics.com Date: Fri, Jan 4, 2013 at 5:32 PM Subject: Re: [Histonet] Helicobater Pylori QC block and/or slides To: Tasha Fraser tfra...@olympicmedical.org We have a human colon tissue that is positive. On Fri, Jan 4, 2013 at 12:37 PM, Tasha Fraser tfra...@olympicmedical.org wrote: Does anyone have a good suggestion for a good HP control block or slides? Tasha Fraser, HT (ASCP) Olympic Medical Center Port Angeles, WA - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
