[Histonet] Re: Helicobacter stains and controls
There are four broad choices for staining Helicobacter. 1. Rely on H E alone. Some people claim it works, but I can't find them unless they're all over the place. 2. Thiazine dye methods. The simplest is the blue dye mix Diff-Quik II (or a generic equivalent, all of which work in my experience), the next a Giemsa stain, and it's possible to complicate them considerably more. 3. Silver stains such as Steiner's. They work, but they're a lot of trouble and the tissue often falls off the slide. I have almost no experience with them. 4. Immunostains. More expensive to do, but a lot faster for the pathologist to read. I haven't seen a study comparing the sensitivity of these methods. My personal opinion is that I'm OK with Diff-Quik II if I'm seeing one gastric biopsy a day, but I want IHC if I'm going to be seeing ten of them. With the dye method I often have to resort to oil immersion magnification, where with IHC I barely need to use a high-dry lens. Helicobacter controls: Not really necessary, but should be done anyway. You need the co-operation of your pathologist in finding positives suitable for use as controls. One gastrectomy specimen can of course set you up for life, but these are pretty rare these days. Some additional points: many pathologists don't understand that not all bacteria they see in the stomach are Helicobacter - morphologic criteria must be observed. (IHC gets around this problem.) There is another, rather rare Helicobacter that causes peptic ulcer disease, with different morphology (a tight corkscrew rather than a gull wing) - Helicobacter heilmanii. Supposedly IHC picks it up. There's controversy as to whether all gastric biopsy specimens need a stain of some kind, or only the ones with acute inflammation (confusingly called chronic active gastritis). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Pathologists' Names on HE Labels
Pathologist's name on the slide? I'd just like to see the patient's name on the slide. It helps me not mix up cases, but the old-timer histotechnologists really resist it. I guess you can legal-beagle reasons not to put the pathologist's name on the slide, but I don't see anything wrong with it. Seems like initials would suffice. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: PSCA Primary antibody
Hallo Histonetters, Happy New Year!! I'm looking for information on a PSCA (Prostate Specific Cell antigen) antibody that will work both in human and primate. If are are using this antibody, please contact me. Any information would be appreciated. thanks Dusko Trajkovic 858-638-6202 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: CAP guideline for fixation time for ER/PR/HER2
Time of fixation before ER/PR/HER2: I haven't had much luck with getting this information. Nobody wants to deal with the communication issues. Needle biopsy specimens should be popped into formalin as soon as they are out of the patient, and the time recorded. What you can't find out is whether they are willing to fix the specimens before they take the time to X-ray them. Attempts to find out what they're doing are futile. To the OR nurse, a specimen is perfectly fixed all the way through the instant she drops it into formalin, whether a small lumpectomy or a Dolly-sized mammoon. Eventually we're going to have to realize that larger specimens (more than needle biopsy size) require prompt dissection before fixation, if special studies are to be done. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: Pathologists' Names on HE Labels
You can get Sharpies and highlighters in at least 7 different colors. We color code the pathologists (Dr's Red, Blue, Pink, Orange, Purple, Green, and Yellow.) The Sharpies and highlighters are distributed in the appropriate departments, and the slides and paperwork get a quick mark of color to facilitate their delivery to the appropriate pathologist. Dan Schneider Amarillo, TX On Sat, Jan 5, 2013 at 1:37 PM, Bob Richmond rsrichm...@gmail.com wrote: Pathologist's name on the slide? I'd just like to see the patient's name on the slide. It helps me not mix up cases, but the old-timer histotechnologists really resist it. I guess you can legal-beagle reasons not to put the pathologist's name on the slide, but I don't see anything wrong with it. Seems like initials would suffice. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] holly nuclei bat man
Happy New Year everyone! Can we start a discussion once again on what causes holes in nuclei? It is pretty clear in my experience it has something to do with heating at least for me with antigen retrieval. When I do HIER at ph 8 especially I am seeing a lot of holes in nuclei. HE on same tissue alone without HIER/IHC doesn't seem to exhibit this artifact (holes in the nuclei). I bet it can also happen if the tissue is not fixed well enough to protect it from paraffin processing but right now I am seeing it more after HIER, don't see it as much on my IHC slides with no AR or when I use eier instead of hier. Also notice that ph6 HIER and Ph9 HIER are not as bad as when I use Ph8 for the same times and temps. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Fax: 720-859-4110 mailto:pru...@ihctech.net pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
