Re: [Histonet] Voice Recognition Systems
VoiceBrook is an awesome pathology voice recognition system! We use Meditech and it does not require an interface. Michelle From: Ann Specian thisis...@aol.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, January 15, 2013 6:37 PM Subject: [Histonet] Voice Recognition Systems I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Voice Recognition Systems
As an LIS vendor I hear this question quite often. I'd like to make an attempt to address this query for the benefit of the group. To start with, if your APLIS uses a version of Microsoft WORD to enter gross, or any other part of the report for that matter, then Dragon should work pretty much out of the box. There is no 'interface' required. Also, if your APLIS is based on the Visual Basic programming language, it will probably work for that as well. The bottom line here is that Dragon Naturally Speaking will work with quite a few commercially available word processors. If you're interested in voice entry, I'd recommend purchasing the most inexpensive version available and installing it on a single PC. Just try it. As to Dragon itself, there are different versions and the price varies dramatically. The basic 'engine' appears to be the same in all versions, but the 'medical' version adds an Anatomic Pathology specific vocabulary and it will let you create 'macros' and a few other things. For our clients that use Dragon, they all use the medical version. A couple more notes: 1. There's a company out there called Voicebrook who 'repackages' Dragon for lack of a better word. Basically they make Dragon even easier to use and they provide excellent support from what I've heard. I have to be honest though. I've heard they can be expensive. I say that not to criticize their marketing strategy, but to warn the smaller labs with smaller budgets who read histonet. 2. Grossing, in particular, is challenging to voice recognition not because the word choice is difficult (In fact, the 'home' version of Dragon might work fairly well here), but because of the environment -- it's just messy and to get Dragon to truly work well, you'll have to make some corrections. I've seen Dragon work for grossing, but in those places where Dragon worked well for grossing it was because 'templates' were in significant use. Basically, the user would use Dragon to fill in the blanks. If anyone has any further questions please feel free to contact me offline. We have a 20 pathologist, multihospital client who use Dragon 100% for all their diagnoses. Hence, our experience. PS: Your APLIS vendor may charge a license fee to 'allow' you to use Dragon with their product. You should check with them. We do not charge this fee as there really is no effort on our side to enable this functionality for use throughout our entire LIS. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Tuesday, January 15, 2013 3:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Voice Recognition Systems I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Voice Recognition Systems
We use Dragon for micro. We found the gross room took more time editing than transcribing, so we gave up on using it there. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Tuesday, January 15, 2013 6:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Voice Recognition Systems I am looking for a voice recognition system for the gross room. It needs to be a system that will interface with our LIS. Does anyone have any recommendations in regard to vendors? Thank you, Ann Specian ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining
It is polymer. No endogenous biotin-problem. The staining looks quiet specific. It is confined to cytoplasm. And there are clear negativ cells beside faintly stained positiv cells. I'm reading about the envading features of helicobacter into host cells and about the injection of VacA and CagA into the host-cell via pili. Therefore I think, the polyclonal antibody also detects those proteins. But this phenomenon should be a common thing? Also cases are described, where the authors see an infra-nuclear area in gastric mucosa cells, that's metachromatic with Touluidinblue. (I'm not through the whole literature) But in their figures of IHC the intracellular stainings are stronger. Perhaps the bugs change their shape and sit in the cells? Or the positiv cells mean something like: Helicobacter was here. This night I repeat Hp-IHC with longer incubation and amplification to see, if the cellular staining can be enhanced, while the surrounding stays clear. I work at a diploma-thesis with MALT-Lymphoma cases and Hp-detection. And now I came across this funny staining. I will also do a PCR on some of these cases. It will be interesting, if this staining is correlated with a positiv PCR. Bye Gudrun -Ursprüngliche Nachricht- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Mittwoch, 16. Jänner 2013 16:03 An: gu.l...@gmx.at Betreff: Re: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Is that avidin-biotin based or polymer? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Gudrun Lang gu.l...@gmx.at 1/16/2013 12:54 AM We work with ultraview-dab kit on ventana benchmark Ultra. Gudrun -Ursprüngliche Nachricht- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Dienstag, 15. Jänner 2013 23:44 An: gu.l...@gmx.at Betreff: Re: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Which Ventana detection system are you using? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Gudrun Lang gu.l...@gmx.at 1/15/2013 3:56 PM Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining. From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining
Have you checked to see if patient treatment might be a factor? Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, January 16, 2013 10:48 AM To: 'Richard Cartun' Cc: histonet@lists.utsouthwestern.edu Subject: AW: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining It is polymer. No endogenous biotin-problem. The staining looks quiet specific. It is confined to cytoplasm. And there are clear negativ cells beside faintly stained positiv cells. I'm reading about the envading features of helicobacter into host cells and about the injection of VacA and CagA into the host-cell via pili. Therefore I think, the polyclonal antibody also detects those proteins. But this phenomenon should be a common thing? Also cases are described, where the authors see an infra-nuclear area in gastric mucosa cells, that's metachromatic with Touluidinblue. (I'm not through the whole literature) But in their figures of IHC the intracellular stainings are stronger. Perhaps the bugs change their shape and sit in the cells? Or the positiv cells mean something like: Helicobacter was here. This night I repeat Hp-IHC with longer incubation and amplification to see, if the cellular staining can be enhanced, while the surrounding stays clear. I work at a diploma-thesis with MALT-Lymphoma cases and Hp-detection. And now I came across this funny staining. I will also do a PCR on some of these cases. It will be interesting, if this staining is correlated with a positiv PCR. Bye Gudrun -Ursprüngliche Nachricht- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Mittwoch, 16. Jänner 2013 16:03 An: gu.l...@gmx.at Betreff: Re: AW: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Is that avidin-biotin based or polymer? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Gudrun Lang gu.l...@gmx.at 1/16/2013 12:54 AM We work with ultraview-dab kit on ventana benchmark Ultra. Gudrun -Ursprüngliche Nachricht- Von: Richard Cartun [mailto:rcar...@harthosp.org] Gesendet: Dienstag, 15. Jänner 2013 23:44 An: gu.l...@gmx.at Betreff: Re: [Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic staining Which Ventana detection system are you using? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Gudrun Lang gu.l...@gmx.at 1/15/2013 3:56 PM Hi! I need some help with the interpretation of Hp-IHC. I stained several cases of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200, ventana benchmark). Some cases showed the nice bacteria and had usually a clean overall background. But some cases showed no bacteria but slight cellular staining of epithelial cells. It was confined on the cells, and usually not all cells in the biopsy had this staining. From literature I learned, that Hp brings its toxine and other proteins (CagA) into the hostcell. Is it possible, that the polyclonal antibody detects this stuff, although hardly an intact bacterium can be seen? Anybody with similar findings? Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all
[Histonet] Re: Helicobacter pylori immunocytochemistry - cytoplasmatic staining
I quite often see faint cytoplasmic staining in immunostains for Helicobacter. You're supposed to ignore it. It's often present in the negative control slide - one of the few reasons I ever look at a negative control slide. These days I get my IHC for Helicobacter from whatever Genzyme is called this week. I'm noticing considerably less cytoplasmic staining than I've usually seen before. (I have no commercial interest here.) Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] interesting photography
A time-lapse movie showing the immune response in the lymph nodes of a mouse edged out a fruit fly sperm fight for top honors at this year's Nikon Small World in Motion Photomicrography competition. http://www.scientificamerican.com/video.cfm?id=art-and-science-come-together-in-ni2013-01-15 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Which fixative for eyes
Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Which fixative for eyes
I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguern...@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro lou...@princeton.huntingdon.com wrote: Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Which fixative for eyes
We work with mice and do a variety of fixatives, but we prefer a methanol/acetic acid fixative. It holds the shape of the eye very well, although it may not show the Schlemm's canal as well as other fixatives. -Liz -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lucie Guernsey Sent: Wednesday, January 16, 2013 1:59 PM To: Louro, Pedro Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Which fixative for eyes I'm curious about this as well. We work with mice and rats in our lab and whenever we need eyes (corneas, more specifically), we immersion fix them whole in Bouin's solution overnight at 4C then transfer to 70% EtOH until we're ready to excise the corneas, process and embed into paraffin. We've found that we get nice half-moon sections with good looking stromal and epithelial layers this way compared to fixing the eyes in 4% PFA (which yielded squiggly, damaged corneas). We then perform IHC. Would love to know if there's a better way, even though this seems to work for us. Thanks, Lucie Lucie Guernsey UCSD lguern...@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro lou...@princeton.huntingdon.com wrote: Hello to the eye experts, I am currently working on a large animal (LA) eye project which will give me the flexibility of experimenting with different fixatives. I am currently using Modified Davidson's solution (48 hours) followed by a 10% NBF solution prior to trimming the eyes, embedding , microtomy and HE staining. After literature research, I found that different labs are using: Modified Davidson's solution Davidson's solution 10% NBF Gluteraldehyde Some places inject the eye with fixative before submerging the eye itself. I wanted to get some ideas / preferences from the experts out there so I can cover my entire basis before finalizing my conclusions. Thanks in advance to all, Pedro Louro Group Leader Histology / IHC ** ** Our Values: Excellence, Drive, Ownership, Challenge, Teamwork, Respect ** ** LEGAL NOTICE This message is confidential and contains information which may be legally privileged. It is intended for the stated addressee(s) only. Access to this email by anyone else is unauthorised. If you are not the intended addressee, any disclosure or copying of the contents of this email or any action taken (or not taken) in reliance on it is unauthorised and is unlawful. If you are not the addressee, please inform the sender immediately and destroy the original message. The data in this email will have been screened for the presence of computer viruses known by the Company at the time the email was produced. The Company cannot guarantee, however, that the email or any attachments are virus free. Delivery of the email will be at the Client's risk. Registered in England No. 1815730 VAT Reg. No. GB425507072 Registered Office: Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
