[Histonet] Thrombin
I am trying to purchase thrombin for cell block preparation, and have been running into dead ends. Can someone give me their vendor information? Laurie Best Histology Supervisor Sunrise Medical Labs 250 Miller Place Hicksville, NY 11801 (631)435-1515x1018 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy or take any action in reliance on it. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] SDH methods for muscles
Hey Julia... WE do SDH routniely on our muscles every week and they work just fine. Can you forward your protocol? Id like to see the difference in them if any? I remain yours truely, Candice Camille From: Celebre Julia celeb...@hhsc.ca To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Friday, January 25, 2013 1:00 PM Subject: [Histonet] SDH methods for muscles I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Shandon HistoCentre II question
I know most embedding machines put out heat due to the block warming chamber, paraffin vat and compressor of cooling consol. But those of you who have experience with this machine, do you think it seems hotter than other or does it have issues with retaining a constant temperature? Any thoughts.. Nicole Tatum, HTL ASCP ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Robin Simpkins
Does anyone have the phone number and/or email for Robin Simpkins at Biocare? Thanks, Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TBS tissue processor single mode vs group mode
Hello All, We just got a TBS APT1-120 tissue processor donated to us. I am would like to hear from anyone about the single mode verse the group mode of operation. What your opinoins about using one verse the other. I hope to set this up this week and would like advice on which is better. Thank you -- Hans B Snyder Histologistics 100 Barber Ave Worcester, MA 01606 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about slide drying/ convection ovens
Hi everyone, Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question about slide drying/ convection ovens
Natalia: I just cannot understand the concept of gravity convection oven because if you say gravity by definition that will mean that the heat will work by gravity and any heated air goes, also by definition, against gravity because any heat air will go UP and not down, hence the hot air balloons. Probably the title is just a catchy but wrongly selected sales mimic, something new and different. Other than that, and going to your question, no I have never used such an oven. Ovens are of the convection type and in all of them the heating elements are at the bottom and the heated air goes UP. René J. From: Natalie Nagy nagy_nata...@holyokehealth.com To: histonet@lists.utsouthwestern.edu Sent: Monday, January 28, 2013 12:40 PM Subject: [Histonet] Question about slide drying/ convection ovens Hi everyone, Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ThrombinIn-Reply-To=
Dear Laurie, We use thrombin and plasma to form our cell blocks. We order the Topical Thrombin 5,000 IU from King Pharmaceuticals. Hope this helps, Natalie Nagy (HT)ASCP Holyoke Hospital Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Question about slide drying/ convection ovens
There is a reason to distinguish the type of oven but the terms are used loosely and so cause confusion. Convection is used to describe in nature to describe the natural circulation of heat and can be of natural gravity convection in which heat rises, or forced in which some kind of external force spreads the heat faster Ie, wind. Strictly speaking a Convection Oven is one that uses a fan to distribute the heat. That is opposed to Conventional Ovens that do not use fans and rely on gravity to distribute heat (heat rises, setting up convection currents). However, if you look at equipment catalogs you will see terms like Gravity Convection and Forced Air to distinguish between ovens without and with fans respectively. Most ovens in use are gravity-convection ovens that rely on the natural rise of heated air to circulate the heat. They don' t use fans. In contrast is the forced-air convection oven that uses a fan to circulate air and ensure even heating throughout the oven. Forced air can heat/dry a sample faster if the fan is set to circulate air at a faster rate than would happen by natural convection. So, the moral of the story is to read the description of the oven to find out exactly what kind of oven it really is! Tim Morken UCSF Pathology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, January 28, 2013 10:06 AM To: Natalie Nagy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question about slide drying/ convection ovens Natalia: I just cannot understand the concept of gravity convection oven because if you say gravity by definition that will mean that the heat will work by gravity and any heated air goes, also by definition, against gravity because any heat air will go UP and not down, hence the hot air balloons. Probably the title is just a catchy but wrongly selected sales mimic, something new and different. Other than that, and going to your question, no I have never used such an oven. Ovens are of the convection type and in all of them the heating elements are at the bottom and the heated air goes UP. René J. From: Natalie Nagy nagy_nata...@holyokehealth.com To: histonet@lists.utsouthwestern.edu Sent: Monday, January 28, 2013 12:40 PM Subject: [Histonet] Question about slide drying/ convection ovens Hi everyone, Just have a quick question for all of you out there in histo-land. Have you ever bought or worked with a gravity convection oven (the thermo shandon 20GC), for drying of slides?? If so..how was it? Did it properly dry all of your slides, did it keep temp?? I am thinking about buying one, and wanted some opinions. Or do you all prefer a real forced air slide drying oven? Thanks in advance, Natalie Nagy (HT)ASCP Histology Supervisor Holyoke Medical Center Holyoke, MA. CONFIDENTIALITY NOTICE: This email communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately and destroy all copies of this communication and any attachments. For further information regarding Holyoke Medical Center's privacy policy, Please visit our Internet web site at http://www.holyokehealth.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CSF non-GYN testing
Hi Ian, You have a good question but can you tell us a little more about your specimen type? Are you processing these specimens in a fluid based medium (ie, Thin Prep) or making some cytospins? Any types of bodily fluids that are collected will vary in cellularity and any microbes that could be potentially present in the patient sample. It is very difficult to pinpoint a volume that would be appropriate for collection. Sorry if you already know this - CSF samples are generally collected in multiple tubes and divided through the various departments of the clinical lab and histology based on the battery of tests the physician has requested. Particularly for CSF, I would request that any and all available remaining samples from the clinical laboratory be given to histology for processing in addition to the histology aliquot for processing. Some of the CSF samples are very acellular and you'll have a better chance at identifying whatever disease process may be present with a greater volume of CSF sample. Christina Thurby Bristol Myers Squibb 812-307-2093 Message: 1 Date: Sun, 27 Jan 2013 19:29:04 + From: Ian R Bernard ibern...@uab.edu Subject: [Histonet] CSF GYN Testing To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d4f4c602b10b9f45b4e9271af6380e161817a...@uabexmb1.ad.uab.edu Content-Type: text/plain; charset=us-ascii What is a perfect amt/size specimen for CSF for Cytology non gyn testing per the literature? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil red O stain on formalin fixed liver
We have an autopsy on a young child. I have a working diagnosis of possible Reye's syndrome and need an Oil Red O stain on a section of formalin fixed liver (not processed) to confirm microvesicular hepatic steatosis. Does anyone do this stain, and could I send the tissue or unstained slides for you to perform the stain and send islides back for me to interpret? I can provide a bone marrow smear to use as a positive control slide, pay shipping both directions and a technical fee. Thank you, David M. Borel, M.D. Pathology Services, P.A. Topeka, KS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: SDH methods for muscles
I have found that the major problem with most of these enzymehistochemical stains is that the sections should not be allowed to sit too long at room temperature before staining. Either stain immediately on sectioning or store at -70oC. The enzymes tend to deteriorate quite rapidly at room temp. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Saturday, 26 January 2013 6:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] SDH methods for muscles I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Thrombin for cell block preparations
Laurie Best, Histology Supervisor at Sunrise Medical Labs, Hicksville NY asks: I am trying to purchase thrombin for cell block preparation, and have been running into dead ends. Can someone give me their vendor information? I know about using thrombin to clot plasma to form cytopathologists' cell blocks, but have never seen it done, and don't know a source. In the many labs I've worked in, I've found that cell block methods and results vary greatly from lab to lab, from labs that don't do them at all, to labs that do them routinely on pleural and peritoneal fluids with uniformly good results. If I were setting up a procedure, I'd probably try the commercial agar preparation Histogel. Cell blocks are a necessary cytologic preparation, the more so as the use of immunohistochemistry increases. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oil red O stain on formalin fixed liver
David, (From Doug Reye's old Laboratory). I have found the following technique the best: Cryotomy of Formalin Fixed Tissues Sometimes frozen sections are requested on tissue that has been fixed in 10% formalin. Cutting frozen sections of formalin fixed tissue can be frustrating at times due to shredding of the tissue or failure of the sections to stay on the slides. The following procedure makes sectioning a lot easier: Solutions: 1. OCT frozen embedding compound (or equivalent) 2. OCT Infiltration Solution: OCT Compound4ml Distilled Water 8ml 3. Sticky Slides (Poly Lysine or equivalent) Method: 1. Take well-fixed tissue and gently wash in tap water 1hr. 2. Place in OCT Infiltration Solution and place on rotor. * For small pieces (needle or thin wedge) 2hr. * For larger pieces 4hrs - overnight. 3. Gently blot excess solution from tissue and embed in OCT and freeze. 4. Cut frozen sections and pick up on adhesive coated slides. 5. Place one slide in Methanol and stain HE or air-dry sections for oil red O staining Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dmborel Sent: Tuesday, 29 January 2013 6:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Oil red O stain on formalin fixed liver We have an autopsy on a young child. I have a working diagnosis of possible Reye's syndrome and need an Oil Red O stain on a section of formalin fixed liver (not processed) to confirm microvesicular hepatic steatosis. Does anyone do this stain, and could I send the tissue or unstained slides for you to perform the stain and send islides back for me to interpret? I can provide a bone marrow smear to use as a positive control slide, pay shipping both directions and a technical fee. Thank you, David M. Borel, M.D. Pathology Services, P.A. Topeka, KS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
