[Histonet] RE: Leica Bond III Opinions
I would love to hear any feedback that you get from this query. Michelle M Lamphere, HT (ASCP) Senior Tech, Histology Children's Medical Center 1935 Medical District Drive Dallas, TX 75235 Office :214-456-2798 Histology: 214-456-2318 Fax: 214-456-0779 Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: High complexity test
Hey Kathy, It is not considered a High Complexity test on the staining side of it. You don't need any special degree or certification, I know we all want our profession to fetch more money and respect and a good way is make a mountain out of molehill to use an old saying. Now here is the gray area, the high complexity could come with the resulting of the stains, such as ER, PR, HER2 using an semi-quantitative detection and algorithms. The simple robotics of staining is not considered high complexity, you do need knowledge and understanding of how detection kits work and antibodies react so and so forth, but its still not considered high complexity. Best way is to call CAP or whatever governing body your under and ask the question and document who you spoke with if there is nothing in writing. Tim H. From: Sara Baldwin/mhhcc.org sbald...@mhhcc.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, February 6, 2013 2:54 PM Subject: [Histonet] High complexity test Hi histonetters Is ventana Ultra IHC only doing antibodies no FISH or CISH is this considered High complexity testing? We are doing ER/PR and some others. Thanks Histology/Cytology Supervisor S. Kathy Baldwin, SCT (ASCP) Memorial Hospital and Health Care Center sbald...@mhhcc.org Ph 812-996-0210, 0216, Fax 812-996-0232, Pager 812-481-0897, Cell 812-887-3357 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor dehydration cycles..
Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] embedding problem
I had to sign up again for the Histonet so I am not sure if this question went out so will resend. thanks Hello Histonetters, We are trying to embed a polycarbonate device with soft tissue attached to look at the implant interface. The problem is that with several standard protocols for pmma processing the clearing agent (methyl salicylate or xylene) and the methacrylate monomer dissolves the polycarbonate. Does anyone have any experience trying process such a thing, an embedding media (pmma, OCT for cryo, etc.), and then a method of sectioning it keeping the interface intact? Thanks in advance for any suggestions! John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] High complexity test
I only hope that Tim's posting sets this issue to rest. The histotech doing IHC, FISH, or grossing and some other complex tasks has to have special training and studies because all those are high complexity tests. Even those histotechs reading FISH results (counting the reactive nuclei for latter signing by the pathologist) have to receive special training. I will just point out again that some administrators try to underrate these tests in order to train their monkeys and pay less even when the ratio billing/cost is extremely high. Management greed drives sometimes these issues. René J. From: Morken, Timothy timothy.mor...@ucsfmedctr.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, February 6, 2013 5:47 PM Subject: RE: [Histonet] High complexity test The CLIA definition of High Complexity testing is not absolute, rather High Complexity Testing is determined by a scored algorithm of the entire Test System (preanalytical through Post Analytical). As such, it clearly takes into account the laboratory or other personnel performing all the specimen collection, grossing, processing, cutting in the pre-analytical phase, and the Testing Personnel (CLIA Definition) performing the analytical phase (preparing slides, reagents, applying reagents, quality control, etc) and post analytical phase(interpretation) . The Pathologist's role is only part of that and is scored accordingly. CLIA clearly considers the IHC Test System as High Complexity and requires a technologist for the IHC portion with at least and Associates degree (or equivalent, including course and experience in appropriate science and testing) for Testing Personnel. So, I don't think it is correct to dismiss any personnel standards as irrelevant simply because a pathologist will do the interpretation. Note that if ANY High Complexity tests are performed in the lab then the lab must have a CLIA certification for High Complexity Testing. References: CLIA website with the personnel regulations: http://wwwn.cdc.gov/clia/regs/subpart_m.aspx CAP QA about personnel standards for IHC, ISH and IF http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf Relevant IHC question reproduced below: Q 17. My question refers more specifically to immunofluorescence, in situ hybridization and immunohistochemistry. Are the techs that perform these tests considered high complexity testing personnel? If the techs are reporting any kind of preliminary result, they must be qualified to do high complexity testing. If all they are doing is applying the stain, then that is considered processing. A: Personnel performing immunofluorescence, immunohistochemistry and in-situ hybridization techniques require qualifications applicable to high complexity testing. Personnel performing histology processing using routine standardized staining procedures (not classified as molecular) do not fall under CLIA as testing personnel and do not have qualification requirements define CLIA website detailing test categorization: http://wwwn.cdc.gov/clia/regs/subpart_a.aspx#493.17 Excerpt here about High vs Moderate complexity (low complexity are basically home use tests) Sec. 493.17 Test categorization. (a) Categorization by criteria. Notices will be published in the Federal Register which list each specific test system, assay, and examination categorized by complexity. Using the seven criteria specified in this paragraph for categorizing tests of moderate or high complexity, each specific laboratory test system, assay, and examination will be graded for level of complexity by assigning scores of 1, 2, or 3 within each criteria. The score of 1 indicates the lowest level of complexity, and the score of 3 indicates the highest level. These scores will be totaled. Test systems, assays or examinations receiving scores of 12 or less will be categorized as moderate complexity, while those receiving scores above 12 will be categorized as high complexity. Note: A score of 2 will be assigned to a criteria heading when the characteristics for a particular test are intermediate between the descriptions listed for scores of 1 and 3. (1) Knowledge. (i) Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction. (ii) Score 3. Specialized scientific and technical knowledge is essential to perform preanalytic, analytic or postanalytic phases of the testing. (2) Training and experience. (i) Score 1. (A) Minimal training is required for preanalytic, analytic and postanalytic phases of the testing process; and (B) Limited experience is required to perform the test. (ii) Score 3. (A) Specialized training is essential to
[Histonet] Xylene/Paint Thinner
If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Processor dehydration cycles..
You have to be careful or the salts will precipitate out of the formalin if you start too high. I wouldn't go any higher than 70. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 07, 2013 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor dehydration cycles.. Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processor dehydration cycles..
I usually started with 80%EthOL but it does not harm starting with 70%EthOL and it is even better, from the theoretically view point. So if you have the space in your protocol, keep the 70%EthOL René J. From: Tom McNemar tmcne...@lmhealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 10:14 AM Subject: [Histonet] Processor dehydration cycles.. Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: embedding problem
John Have you tried paraffin? I know that we have been able to section some plastic devices with paraffin sections, it may be worth a try. Looks like the device you are working with is compatable with xylene, we have found in some cases that we need to use a xylene subsititute. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 881-0763 cell (303) 682-9060 fax l...@premierlab.com Ship to address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Baker, John [bak...@med.umich.edu] Sent: Thursday, February 07, 2013 8:19 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding problem I had to sign up again for the Histonet so I am not sure if this question went out so will resend. thanks Hello Histonetters, We are trying to embed a polycarbonate device with soft tissue attached to look at the implant interface. The problem is that with several standard protocols for pmma processing the clearing agent (methyl salicylate or xylene) and the methacrylate monomer dissolves the polycarbonate. Does anyone have any experience trying process such a thing, an embedding media (pmma, OCT for cryo, etc.), and then a method of sectioning it keeping the interface intact? Thanks in advance for any suggestions! John John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 109 Zina Pitcher Place, 2218 BSRB Ann Arbor, MI 48109-2200 734-936-1635 ** Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene/Paint Thinner
For sure they do not know, I have asked a few painters. As many histotechs, they have told me that the like the smell of xylene. René J. From: White, Lisa M. lisa.whi...@va.gov To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 10:23 AM Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Xylene/Paint Thinner
I have to admit, I don't mind the smell:) But I also like the smell of gasoline, and I know both are bad for me! Thanks again to everyone who has offered feedback on this topic. Rene, I have printed out a few of the articles you've written and am anxious to research these methods more. Thanks a bunch! Best, Adrienne On Feb 7, 2013, at 10:36 AM, Rene J Buesa wrote: For sure they do not know, I have asked a few painters. As many histotechs, they have told me that the like the smell of xylene. René J. From: White, Lisa M. lisa.whi...@va.gov To: histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 10:23 AM Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Xylene/Paint Thinner
I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Xylene/Paint Thinner
I'm sure my wife would love that. Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edumailto:vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Thursday, February 07, 2013 8:14 AM To: Victor A. Tobias; 'White, Lisa M.'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Xylene/Paint Thinner If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wash with running tap water and dry. They will degrease even better tan with xylene. René J. From: Victor A. Tobias vtob...@uw.edu To: 'White, Lisa M.' lisa.whi...@va.gov; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edumailto:vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Xylene/Paint Thinner
If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wash with running tap water and dry. They will degrease even better tan with xylene. René J. From: Victor A. Tobias vtob...@uw.edu To: 'White, Lisa M.' lisa.whi...@va.gov; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Processor dehydration cycles..
I agree with Joyce on this: Formalin salt precipitate tends to become more common if you start above 70%. WE use a 70%, then 80% and two 95% in our process here. Very Respectfully, Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology 959 CSPS/ SGVLH WHASC JBSA-Lackland AFB, TX 78236 (210) 292-5519 DSN: 662-5519 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Xylene/Paint Thinner
And Dawn is the best... And I am in no way linked to this product!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, February 07, 2013 11:14 AM To: Victor A. Tobias; 'White, Lisa M.'; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] RE: Xylene/Paint Thinner If you ever need to degrease any auto part, just place it in water, add liquid soap to about 5% conc., and heat it until boiling and let them in boiling water during 5 minutes. Wash with running tap water and dry. They will degrease even better tan with xylene. René J. From: Victor A. Tobias vtob...@uw.edu To: 'White, Lisa M.' lisa.whi...@va.gov; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Thursday, February 7, 2013 11:06 AM Subject: [Histonet] RE: Xylene/Paint Thinner I don't know if the auto parts stores carry xylene, but it is an awesome degreaser. Learned that from my mentor back in the 70's. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of White, Lisa M. Sent: Thursday, February 07, 2013 7:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene/Paint Thinner If you look on the paint thinner isle at your local Lowe's. You will find Xylene on the shelf. The first time I saw it was a shock. Wonder if the home improvement weekend warriors know what they are getting all over their hands? Lisa White, HT(ASCP) Supervisory HT James H. Quillen VAMC PO Box 4000 Corner of Veterans Way and Lamont PLMS 113 Mountain Home, TN 37684 423-979-3567 423-979-3401 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Processor dehydration cycles..
Tom, We start at 80%. Tim Morken UCSF Pathology -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Thursday, February 07, 2013 7:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor dehydration cycles.. Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] High complexity test
FISH is definitely high complexity. The tech who scores the slide must meet the qualifications to perform high complexity testing. I understood Kathy's question to be about the person who loads and runs the slide stainer. I still say give CAP or whomever you accrediting agency is a call and see what they say. Histonet advice is just that. Mark On Thu, Feb 7, 2013 at 7:22 AM, Rene J Buesa rjbu...@yahoo.com wrote: I only hope that Tim's posting sets this issue to rest. The histotech doing IHC, FISH, or grossing and some other complex tasks has to have special training and studies because all those are high complexity tests. Even those histotechs reading FISH results (counting the reactive nuclei for latter signing by the pathologist) have to receive special training. I will just point out again that some administrators try to underrate these tests in order to train their monkeys and pay less even when the ratio billing/cost is extremely high. Management greed drives sometimes these issues. René J. From: Morken, Timothy timothy.mor...@ucsfmedctr.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, February 6, 2013 5:47 PM Subject: RE: [Histonet] High complexity test The CLIA definition of High Complexity testing is not absolute, rather High Complexity Testing is determined by a scored algorithm of the entire Test System (preanalytical through Post Analytical). As such, it clearly takes into account the laboratory or other personnel performing all the specimen collection, grossing, processing, cutting in the pre-analytical phase, and the Testing Personnel (CLIA Definition) performing the analytical phase (preparing slides, reagents, applying reagents, quality control, etc) and post analytical phase(interpretation) . The Pathologist's role is only part of that and is scored accordingly. CLIA clearly considers the IHC Test System as High Complexity and requires a technologist for the IHC portion with at least and Associates degree (or equivalent, including course and experience in appropriate science and testing) for Testing Personnel. So, I don't think it is correct to dismiss any personnel standards as irrelevant simply because a pathologist will do the interpretation. Note that if ANY High Complexity tests are performed in the lab then the lab must have a CLIA certification for High Complexity Testing. References: CLIA website with the personnel regulations: http://wwwn.cdc.gov/clia/regs/subpart_m.aspx CAP QA about personnel standards for IHC, ISH and IF http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf Relevant IHC question reproduced below: Q 17. My question refers more specifically to immunofluorescence, in situ hybridization and immunohistochemistry. Are the techs that perform these tests considered high complexity testing personnel? If the techs are reporting any kind of preliminary result, they must be qualified to do high complexity testing. If all they are doing is applying the stain, then that is considered processing. A: Personnel performing immunofluorescence, immunohistochemistry and in-situ hybridization techniques require qualifications applicable to high complexity testing. Personnel performing histology processing using routine standardized staining procedures (not classified as molecular) do not fall under CLIA as testing personnel and do not have qualification requirements define CLIA website detailing test categorization: http://wwwn.cdc.gov/clia/regs/subpart_a.aspx#493.17 Excerpt here about High vs Moderate complexity (low complexity are basically home use tests) Sec. 493.17 Test categorization. (a) Categorization by criteria. Notices will be published in the Federal Register which list each specific test system, assay, and examination categorized by complexity. Using the seven criteria specified in this paragraph for categorizing tests of moderate or high complexity, each specific laboratory test system, assay, and examination will be graded for level of complexity by assigning scores of 1, 2, or 3 within each criteria. The score of 1 indicates the lowest level of complexity, and the score of 3 indicates the highest level. These scores will be totaled. Test systems, assays or examinations receiving scores of 12 or less will be categorized as moderate complexity, while those receiving scores above 12 will be categorized as high complexity. Note: A score of 2 will be assigned to a criteria heading when the characteristics for a particular test are intermediate between the descriptions listed for scores of 1 and 3. (1) Knowledge. (i) Score 1. (A) Minimal scientific and technical knowledge is required to perform the test; and (B) Knowledge required to perform the test may be obtained through on-the-job instruction.
[Histonet] Processor dehydration cycles
Hi Tom: I deal exclusively with post-mortum brain tissue, so my situation may not apply to you. I do not use formalin on my processor, since the half brain used for brain-cutting has already been thoroughly fixed. So, I have the luxury of using 30%, 50%, 80%, 95%, then three 100% Isopropanols. They used this protocol when I was at a different neuropathology laboratory. I believe the rational for this was that starting with a lower concentration of alcohol, and then more gradually increasing the concentrations, would reduce the concentration gradient between the cells and the solution, and so avoid strong currents from harming the cells. Also, brain tissue may be more delicate that say prostate, skin or uterus. By the way, I actually do not know whether this rational is correct or not. However, in general, if you can start at 70%, it can't do any harm. Tim Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA (617) 855-359 Tom McNemar wrote: Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? Thanks in advance. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposal of old blocks Slides
How do you dispose of old tissue blocks after the 10 year hold period? Can old glass tissue slides be sent for recycling? Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things. Kindness is the language the blind can see the deaf can hear- Mark Twain ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Leica Bond III Opinions
Forwarding a message I received: Hi Roger, I've been using the Lieca Bonds for about 4 years now and I wouldn't trade for anything. The lab I am currently at changed everything from Ventana over to Bonds about a year and a half ago, and we have NO regrets! Bonds are faster, more flexible with protocols, and have been producing higher quality staining. The test is also cheaper on Bonds than on Ventana. For me, I think the flexibility with protocols and the reliable, consistent, good staining is the real bonus. Your questions: Are you happy with the quality of the stains you're performing on the Bond III? - ABSOLUTELY, NO REGRETS Are there antibodies that you were not able to run on the Bond III? - NONE SO FAR. I USE ANTIBODIES FROM VARIOUS SUPPLIERS, I DON'T LIMIT MYSELF TO JUST LEICA ANTIBODIES Is the machine reliable, or does it break down often, requiring technical service? - RARELY NEEDS TECHNICAL SERVICE, I DO NOTE THAT KEEPING THE SLIDE ASSEMBLY PADS CLEAN (ESPECIALLY THE DRAIN HOLES) IS IMPORTANT FOR OPTIMAL STAINING. WE DO A GOOD CLEANING MONTHLY How arduous is cleaning and managing the cover tiles? SIMPLE ENOUGH. USED TILES ARE SLID INTO A RACK SITTING IN DISTILLED WATER AFTER USE. WHEN THE RACK IS FILLED, WE ADD SOME BLEACH, LET SIT FOR 20 - 3O MINUTES, RINSE THE WHOLE RACK WELL IN DISTILLED, RINSE IN ABSOLUTE ALCOHOL, SIT OUT TO DRY . Are the three black slide drawers durable? I'VE NEVER HAD ONE BREAK OR WARP. Anything else I can answer? Beth Cox, HTL/SCT(ASCP)QIHC Message: 7 Date: Wed, 06 Feb 2013 13:15:35 -0600 From: Roger Heyna rhe...@lumc.edu Subject: [Histonet] Leica Bond III Opinions To: histonet@lists.utsouthwestern.edu Message-ID: 51125777022300049...@gwgwia1.luhs.org Content-Type: text/plain; charset=us-ascii Could I get the opinions of any labs using the Leica Bond III for IHC staining? I'm especially interested to hear from those that switched from Ventana to Leica, but any feedback is appreciated. Few questions that come to mind: Are you happy with the quality of the stains you're performing on the Bond III? Are there antibodies that you were not able to run on the Bond III? Is the machine reliable, or does it break down often, requiring technical service? How arduous is cleaning and managing the cover tiles? Are the three black slide drawers durable? Thank you, Roger Heyna Maywood, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processor dehydration cycles
-Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Hello all, I was wondering what most people use as the first reagent after the formalins on their tissue processor? We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration? We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between our Formalin and alcohols. 10% NBF, 10% NBF, PenFix, PenFix and then the alcohols starting at 95%. Speaking of 10% NBF has anyone ever used un-buffered formalin for routine processing? I heard of a place using un-buffered formalin and wondered if 1) would that be okay or is it harsh on tissue and 2) would it keep the salt deposits down? Helayne Parker, H.T. (ASCP) Pathology Section Head Cox Medical Center Branson P.O. Box 650, Branson, MO 65615 Phone: 417-335-7254 Fax: 417-335-7127 Email: hpar...@skaggs.net Web: www.coxhealth.com/branson CoxHealth – ranked one of Missouri's Best Hospitals by U.S. News World Report COXHEALTH CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryostat decontamination
Has anyone come up with a documented and referenced procedure for decontamination of a cryostat using 37% Formaldehyde? I pulled up old notes in the Histonet archives and saw that Tim Morken was working on this but that was a number of years ago. Thanks, Kim Kimberly D. Kolman, HT, (ASCP) Diagnostics 115 VA Eastern Kansas Health Care System 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 ph: 913-682-2000 x 52537/52539 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Double staining with IF/IHC - chromogenic
Hello everyone, I have a co-worker in the research department that would like to know if anyone has ever combined and IF stain with chromogenic IHC staining. His theory for this is that IF shows better nuclear staining from an image analysis stand point. If you have heard of this can you send me literature on it, or if you've done this what is your protocol? It would seem that doing IF first than IHC might cause quenching of the IF, but then again doing chromogenic staining first might cover the IF antigen. I suspect that only an chromogenic cytoplasmic IHC stain can be combined with a nuclear IF marker with IHC first. Any ideas? Again this is specifically from an image analysis stand point. Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartw...@cshs.org IMPORTANT WARNING: This message is intended for the use of the person or entity to which it is addressed and may contain information that is privileged and confidential, the disclosure of which is governed by applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this information is STRICTLY PROHIBITED. If you have received this message in error, please notify us immediately by calling (310) 423-6428 and destroy the related message. Thank You for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Disposal of old blocks Slides
Joe, We redbag all paraffin blocks for medical waste disposal. We use large sharps containers for disposal of glass slides. Very Respectfully, Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology 959 CSPS/ SGVLH WHASC JBSA-Lackland AFB, TX 78236 (210) 292-5519 DSN: 662-5519 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jmasla...@stpetes.org Sent: Thursday, February 07, 2013 11:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of old blocks Slides How do you dispose of old tissue blocks after the 10 year hold period? Can old glass tissue slides be sent for recycling? Thanks in advance Joe Maslanka BS, CT,HT (ASCP) Anatomical Pathology Technical Supervisor St Peter's Hospital,MT 59601 (P)(406) 447-2406 (F)(406)444-2126 Give thanks for ALL things. Kindness is the language the blind can see the deaf can hear- Mark Twain ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN4 staining
Anybody else having problems with PIN 4 staining (Biocare)? We are getting brown background staining, even on the glass itself where there is no tissue. After many years of fantastic results, this problem has occurred recently. Our AMACR is also a lot fainter than it used to be. We've switched out all lots of reagents involved, even bought distilled water. Still no movement towards solving this problem. Any ideas? Jeff Jurczak Uropartners Laboratory Westchester, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 111, Issæue 8
Sent from my Verizon Wireless BlackBerry -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sender: histonet-boun...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 111, Issue 8 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Xylene Substitutes (Blazek, Linda) 2. Graveyard Histotech Position in Spokane, Washington (Miranda Giorgi) 3. RE: Histonet Digest, Vol 111, Issue 7 (Parker, Helayne) -- Message: 1 Date: Wed, 6 Feb 2013 12:13:40 -0500 From: Blazek, Linda lbla...@digestivespecialists.com Subject: RE: [Histonet] Xylene Substitutes To: Adrienne Anderson rennie1...@yahoo.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 5a2bd13465e061429d6455c8d6b40e3916490a9...@ibmb7exchange.digestivespecialists.com Content-Type: text/plain; charset=us-ascii We have used Formula 83 from CBG for years with excellent results. We use it for processing and staining. We also recycle it making the cost very low. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Wednesday, February 06, 2013 11:47 AM To: Adrienne Anderson; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene Substitutes We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with excellent results as well as cost savings. Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Adrienne Anderson [mailto:rennie1...@yahoo.com] Sent: Tuesday, February 05, 2013 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene Substitutes Hello all, My lab is looking into xylene substitutes, and I'd love some feedback on what other labs are using. We currently use SubX, but are there other items out there more economical? Thanks, Adrienne _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 6 Feb 2013 17:18:22 + From: Miranda Giorgi mgio...@icplab.com Subject: [Histonet] Graveyard Histotech Position in Spokane, Washington To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 46bb82e6fc36e44fb454e00c82dbdc56cd1...@icpmail.pai.e-pathology.com Content-Type: text/plain; charset=us-ascii Hello All, Please see the job posting below for a Histotech position at InCyte Pathology in Spokane, WA. Only interested candidates should reply. Please do not respond if you are from a recruitment service. Thank you! Histology Analyst, Graveyard Shift (M-F) InCyte Pathology is a full-service, pathologist-owned anatomic pathology laboratory. Our one-hundred employees and twenty-one board-certified pathologists provide exceptional laboratory services to hospitals and physician offices throughout the Pacific Northwest, Montana, and Alaska from our state-of-the-art facility located in Spokane Valley, Washington. We are just a short drive from five major ski resorts, several beautiful lakes, and a variety of outdoor activities. Qualifications: HT certified or HTL registry eligible, and two years of laboratory experience. This position performs all routine procedures in
[Histonet] best fixative for eyes
What are people's thoughts/experience with different fixatives for eyes (rat, mouse and rabbit mostly). We currently use modified Davidson's solution, but the pathologist is considering going to Davidson's. I guess there has been some difficulty with cutting due to the lens not getting fixed well and causing problems. Thank you for sharing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] best fixative for eyes
How long are you leaving the eyes in the mDavidsons? We have found 24 hours works extremely well. -Original Message- From: Robin Dean robin_d...@compbio.com To: histonet histonet@lists.utsouthwestern.edu Sent: Thu, Feb 7, 2013 6:52 pm Subject: [Histonet] best fixative for eyes What are people's thoughts/experience with different fixatives for eyes (rat, mouse and rabbit mostly). We currently use modified Davidson's solution, but the pathologist is considering going to Davidson's. I guess there has been some difficulty with cutting due to the lens not getting fixed well and causing problems. Thank you for sharing ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond III Opinions
Love the bond it does not break down very often for me and service is great i can run all the antibodies i want for me even research animal because i have a more open research unit cleaning the covertiles is a little tedious but we make it work and replace them with new ones often beats disposing of all the waste from liquid cs generated by ventana in my experience ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] P16 on Leica Bond
Really 1:4?___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PFA, postfixing and GalC MBP antibodies
Hello, I have previously cut frozen sections and post-fixed in methanol for 2 minutes and got OK staining using mono- and polyclonal antibodies to the lipid galactocerebroside (Galc). This has also been the case with MBP (myelin basic protein). I am now using PFA fixed tissue and then washing in acetone. This has given me much better tissue structure but now GalC (and I suppose MBP but I haven't tested it yet) immunoreactivity has been lost. I have used the antigen retrieval method of Inoue Wittbrodt (http://www.ncbi.nlm.nih.gov/pubmed/21603650) but this antigen has not been rescued. I have washed in acetone for 2 and 5 minutes. Do you think this is an antigen retrieval/obliteration issue or the galactocerebroside simply being washed out by the acetone? If anyone has had any success with GalC antibodies and PFA fixation I would like to know about it! So far my searches haven't turned up anything useful. Thanks -- Dr Tyrone Genade Department of Human Biology University of Cape Town South Africa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Leica Bond III Opinions
The bond is great. A lot less waste to deal with and the maintenance is s easy. Less than 10 minutes. No tubing to unclog and disinfect. And the operating cost is much cheaper. And to top it off, it won't even give you the option to start a run until everything is right. No going back and forth to fix this, that, and whatever. It's truly a walk away system once you click start. Sent from the iPhone of Kim Tournear On Feb 6, 2013, at 8:56 PM, Patsy Ruegg pru...@ihctech.net wrote: Love the bond it does not break down very often for me and service is great i can run all the antibodies i want for me even research animal because i have a more open research unit cleaning the covertiles is a little tedious but we make it work and replace them with new ones often beats disposing of all the waste from liquid cs generated by ventana in my experience ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
