[Histonet] Luxol Fast Blue +PAS staining on rat spinal cord
Dear Fello histonetters, I am looking for demonstration of demyelination in rat spinal cord paraffin sections. A quick look into the literature revealed use of combination of luxol fast blue and PAS for demonstration of demyelination and myelin breakdown products. Anybody over here will suggest me the procedure for doing this staining. There is also kit from hitobiotec for this staining. Now my questions are 1. Whats your preferred stain for demonstrating demyleination? 2. Any experience of using LFB+PAS for demonstration of the same 3. Any idea about the performance of this staining kit from hitobiotec Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Texas Society for Histotechnology 2013 Symposium/Convention
All, The Texas Society For Histotechnology meeting is April 12-14, 2013 at the JW. Marriott in Houston, Texas. Deadline to book a hotel room is March 21, 2013. Please join us for excellent educational offerings which includes 17 workshops and 3 symposiums. We hope to see you in Houston, Texas! Please visit txsh.org to view the program. If you have any questions or would like a program e-mailed to you please contact: Kathy Dwyer at kdwyer3...@aol.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mismatch Repair Enzymes, do you want to learn more?
CIQC/CAP-ACP SEMINAR: DIAGNOSTIC ihc AND MOLECULAR PATHOLOGy This is a unique opportunity to participate in a symposium dedicated to the future of IHC and molecular diagnostics. For registration go to: http://ciqc-capconference.eventbrite.ca/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] sections falling off
Dear Megha Kumar, Use commercial + charged OR silanized slides. Silanized slides are prepared by cleaning the slides by washing, followed with a rinse in 95% ethanol. 4ml of 3-aminopropyltriethoxy silane is added to 200 ml of acetone and slides are dipped for 30-60 seconds, followed by 60 seconds in agitated distilled water. The coated slides are then dried for 1 hour, and can be boxed for future use. References:Bancroft sixth edition page 694 Regards Muhammad Tahseen Senior Supervisor Histopathology SKMCHRC Hi Histonetters ! Can anyone suggest how to prevent adult mouse skin sections from falling off during in situ hybridization? How to make sections adhere better to slides? I am using poly-lysine coated slides for 5-10 micron thick skin sections. The tissue is fixed in 4% PFA and embedded in paraffin. Please help ! thanks megha -- Megha Kumar, Ph.D. Post-doctoral Research Scholar Sen-Bandyopadhyay Lab Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Kanpur, 208016 India phone - 9695830033 * * * * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 111, Issue 32
Hello, On Thu, Feb 21, 2013 at 8:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: From: Margaryan, Naira nmargar...@luriechildrens.org I am trying to do IHC on FFPE 4 weeks old zebrafish with different Abs. Is there a trick working with zebrafish? I am using the same IHC protocol I always use on human and mouse tissue and my Abs are suppose to work on fish as well as human. I run human and fish sections together with same AR and IHC protocol. By the end I get beautiful staining on human section and or nothing or some fuzzy/fuggy/unclarified/undistinguished reaction. I suggest you prepare some unfixed frozen sections for comparison. If the antibody reacts to the unfixed tissue but not the fixed then you probably need to perform one or another antigen retrieval method. I have been having good success with http://www.ncbi.nlm.nih.gov/pubmed/21603650 For my fish, Nothobranchius, I am now fixing for two days at 4 oC (in PFA) and then decalcifying 15% EDTA (pH 7.3) for three days (the last day in 30% sucrose dissolved into the 15% EDTA) before freezing in liquid nitrogen for sectioning. This method is maintaining the structure beautifully. As regards using the above antigen retrieval method I have both soaked the tissue (after fixing) in the 70 oC pH 9 TRIS and then decalcified as well as decalcified, cryo-protected and sectioned (APTES coated slides) and then performed the antigen retrieval at 70 oC. Both routes work well for my fish and the sections don't detach. If you cannot carefully dissect out the tissue of interest then I suggest decalcifying the tissue. You only need one piece of bone to catch on the blade and rip through the tissue to cause a lot of frustration and waste a specimen. I hope this helps. -- Tyrone Genade Ph.D. Department of Human Biology University of Cape Town South Africa http://tgenade.freeshell.org email: tgen...@gmail.com tel: +27-84-632-1925 (c) Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
