[Histonet] Thionin / Acid fuchsin staining Rat brain
*Hello Histonetters,* * * *I am looking for the staining protocol for thionin/Acid fucshin staining.* * * *I have Rat brain slides which are paraffin embedded and sectioned at 5-7 um. * *I have had a problem to make a Acid fucshin solution as it does not stick to the brain slices.* *Please give proper recipe for the same.* *I tried to search a lot but i didnt find any good article or literature that helps figuring this out.* * * *Would appreciate if someone can help me in this regards.* * * *Thanks,* * Mohit Koladia (B.Pharm, MS Pharm) Graduate Research Scholar, North Dakota State University, Fargo, ND, USA-58102* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] service manual Hacker Mesei 3655 Robotic Coverslipper
We were given a Hacker Mesei 3655 Coverslipper as donation for our research. Unfortunately it falters during operation. We badly need a Service Manual for it to go up and running again. Could you possibly provide us even a PDF copy of service manual for this item?Kindly help us by offering a proposal.Thank you.R. Mateoat rudymateo2...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing for Pin 4 Cocktail
I always thought you could bill for three due to three distinguishable staining patterns. The two cytokeratins can not be distinguished from each other, therefore three charges instead of four (PIN4). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Monday, March 18, 2013 1:11 PM To: Angela K. Bitting; histonet@lists.utsouthwestern.edu; Vanessa Perez Subject: RE: [Histonet] Billing for Pin 4 Cocktail Our billing manager said the same thing. If the antibodies are being applied separately, you can bill for each one. If antibodies are being mixed and applied at the same time, you can only bill for one. It sounds like the difference between a double-stain (two different chromogens) and a single stain using an antibody cocktail. I still don't think we're doing it correctly, because we're billing for three, but at least it sounds like we can bill for two, which seems logical. Have others heard something different? Roger Heyna Maywood, IL Bitting, Angela K. akbitt...@geisinger.edu 3/18/2013 7:49 AM I was given info from a Dako rep last week that if the primary antibodies are applied separately to the slide as is true with Ventana instruments, you can charge for both. I am skeptical. Has anyone else heard this? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, March 15, 2013 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL Debbie Granato debgran...@yahoo.com 3/15/2013 11:00 AM We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
RE: [Histonet] Possible continuing automatic staining problem
What do you mean by muted; can you give a little more description? And what type of tissue, and is this HE? Sheryl Stephenson | Histology Technician -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lisa Sent: Saturday, March 16, 2013 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Possible continuing automatic staining problem Hi We have this continuing problem, don't know if its a staining or possible processing problem, all our slides keep coming out dark blue muted, we have adjusted the times every possible way, tried cutting thinner, we change our solutions regularly on our VIP processing machine. Our automatic strainer is a older tissue Tec but the Dr's have never been overjoyed with the stain, but lately even if we hand stain it, it still looks very muted under the microscope..Please any suggestions out there, Dr histotech's very frustrated. Lisa A Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Billing for Pin 4 Cocktail
Well we use a cocktail, so the antibodies are mixed together and so applied at the same time. All in the same dispenser not two diff one. Vanessa -Original Message- From: Bitting, Angela K. [mailto:akbitt...@geisinger.edu] Sent: Monday, March 18, 2013 7:49 AM To: Vanessa Perez; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail I was given info from a Dako rep last week that if the primary antibodies are applied separately to the slide as is true with Ventana instruments, you can charge for both. I am skeptical. Has anyone else heard this? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Friday, March 15, 2013 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Billing for Pin 4 Cocktail Its actually not allowed already. Any cocktail where all stains are done at one time on one slide can only be charged x1 even if its 2 or more antibodys in the cocktail. One of our reps updated us with our PIN cocktail -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roger Heyna Sent: Friday, March 15, 2013 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing for Pin 4 Cocktail Our lab currently bills for three, and I know of other labs in our area that also bill for three. I have heard rumors that this may not be allowed in the near future. Roger Heyna Maywood, IL Debbie Granato debgran...@yahoo.com 3/15/2013 11:00 AM We have a billing question for the PIN4 Cocktail that we perform on prostate needle biopsies. We currently use the Biocare CK5+CK14+p63 and then add the Biocare P 504S in the lab. How would you bill for this? Would billing for 1 stain be correct or can you bill for 3 stains? Thank you for your input. Debbie Granato ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Lean Principles in Pathology Laboratory
I am currently completing my capstone project through the University of Charleston and would greatly appreciate your help in taking a brief 5 minute survey. This survey is part of my action research paper and relates to various Lean methods which you may have applied in your histology laboratory. All respondents who include their email address will receive a copy of the research results upon completion to help identify how other histology labs have implemented various lean methodologies. Your email address will be used for no other purpose. If you choose not to receive this information, leave this field blank. Thanks! Click here to take surveyhttp://www.surveymonkey.com/s/J9TMZ73 or copy and paste the following link into your web browser. http://www.surveymonkey.com/s/J9TMZ73 Greg L. Good, HT(ASCP) Pathology Technical Manager Frye Regional Medical Center 420 North Center Street Hickory, North Carolina 28601 (828) 315-3680 (Office) (828) 315-3493 (Fax) greg.g...@tenethealth.com NOTICE: The information in this communication is confidential and is directed only to the intended recipient. Please do not forward this communication without my permission. If you have received this communication in error, please notify me immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] KY HT Positions
Please no recruiter calls Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IF on FFPE
Hi everyone, Several people have contacted me regarding the article I mentioned in my post yesterday. Here are the details: Immunofluorescence With Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens AJCP 2013 139:71-78; doi:10.1309/AJCPRZG8EXN7BAID Suozhu Shi, Qingli Cheng, Ping Zhang, Nan Wang, Ying Zheng, Xue-Yuan Bai, and Xiangmei Chen Abstract: Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, κ, and λ between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, κ, and λ staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis. This is all foreign to me - we do IF on derm following an inherited protocol. I've never worked up any IF. If anyone has thoughts on how to apply this to skin I'd appreciate it! Thanks Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p57
Hi everyone, Would anyone be willing to share information about what clone/company/dilution you use for p57? We are looking to use this antibody on formalin fixed/paraffin embedded human placenta. Thanks, Jean Taylor, HT(ASCP)QIHC Immuno Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: p57
Jean, we use Leica's clone Kip2 on placentas on our Bonds; 1:150 Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Taylor, Jean Sent: Tuesday, March 19, 2013 12:19 PM To: 'histonet@lists.utsouthwestern.edu'; 'ih...@googlegroups.com' Subject: [IHCRG] p57 Hi everyone, Would anyone be willing to share information about what clone/company/dilution you use for p57? We are looking to use this antibody on formalin fixed/paraffin embedded human placenta. Thanks, Jean Taylor, HT(ASCP)QIHC Immuno Tech Meriter Health Services Madison, WI -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.commailto:ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.commailto:ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.orgmailto:hi...@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.commailto:ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] special stainers
Our histology laboratory is currently having problems with their specials stains; in particular the silver stains. Can you please tell me what instrumentation others in the field may be using for special stains? Thank you Debra A Ortiz, BS, MT, HT (ASCP) Technical Director, Anatomic Pathology The University of Chicago Medicine 5841 S. Maryland Ave. | Rm. S-631, MC6101 | Chicago, IL 60637 Office: 773-702-8492 Pager: 8633 AT THE FOREFRONT OF MEDICINE(r) http://www.uchospitals.edu http://www.uchospitals.edu/ http://www.uchicagokidshospital.org http://www.uchicagokidshospital.org/ http://www.facebook.com/UChicagoMed http://www.facebook.com/UChicagoMed Twitter: @UChicagoMed This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: special stainers
We use the Dako Artisan. What silver stains are you wanting to automate? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of debra.or...@uchospitals.edu Sent: Tuesday, March 19, 2013 1:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] special stainers Our histology laboratory is currently having problems with their specials stains; in particular the silver stains. Can you please tell me what instrumentation others in the field may be using for special stains? Thank you Debra A Ortiz, BS, MT, HT (ASCP) Technical Director, Anatomic Pathology The University of Chicago Medicine 5841 S. Maryland Ave. | Rm. S-631, MC6101 | Chicago, IL 60637 Office: 773-702-8492 Pager: 8633 AT THE FOREFRONT OF MEDICINE(r) http://www.uchospitals.edu http://www.uchospitals.edu/ http://www.uchicagokidshospital.org http://www.uchicagokidshospital.org/ http://www.facebook.com/UChicagoMed http://www.facebook.com/UChicagoMed Twitter: @UChicagoMed This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this e-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is prohibited. If you have received this e-mail in error, please notify the sender and destroy all copies of the transmittal. Thank you University of Chicago Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Immunofluorescence on FFPE skin
Hi Erin We do direct immunofluorescence staining on FFPE kidney biopsies but instead of microwave antigen retrieval, we use proteinase K (20mg/ml Qiagen Cat # 19131) diluted 1/10 in Tris buffered saline for 20 minutes at Room Temp. We also incubate with the antibody (1/5 dilution) for 90 minutes. Don't remember if our student tried using the microwave (it was a student project) but I would certainly give it a try. There really is no difference with the wattage for a microwave - the trick is to get the solution boiling - THEN start your timing for retrieval. We do our AR for our FISH testing for 20 minutes at half power after it comes to a boil. This allows the solution to keep at near boiling temp but not to over flow the container when it comes back to a boil. The timing for using a pressure cooker would have to be validated - now you are adding an additional parameter of pressure to factor in to bring the solution to a boil. Good luck - hope it works for you. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Monday, March 18, 2013 2:28 PM To: histonet Subject: [Histonet] Immunofluorescence on FFPE skin Hello all, My pathologist gave me a copy of Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections. Has anyone else read the paper who might be willing to give me some basic advice to try working it out? Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker? Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Georgia Society for Histotechnology Jekyll Island HISTOPALOOZA!!
There will be a general membership meeting while we are at Jekyll Island. We are asking all of you to help us plan the 2014 meeting. Next year's theme will be HISTOPALOOZA. A palooza is a big, promotional event that's fun and over the top. We plan to celebrate histotechnology in a big way. So, please join us! You only live once, but if you do it right, once is enough. Mae West Wanda K. Simons President Michael Ayers Immediate Past President Michael Bourgeois Vice President Billie Zimmerman Secretary Shirley PowellTreasurer ***Tune in tomorrow for snippets of Billie's Travel Advisor for the Jekyll Island histology meeting. no fluff, no sprinkles, just the facts. Billie Zimmerman MT(ASCP)QIHC GSH secretary (and Zumba gal) Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Validation of Her2
We are working on validating HER 2 testing in our laboratory. I see that the guidelines state that we should use 25 - 100 cases to complete our validation. In the past we sent our Her2 IHC testing to Clarient and they performed the technical testing and then our pathologists would do the scoring. So for validation we used 25 of the cases we sent to Clarient and then did them in house to compare. Our comparison was nearly 100%. Here is my question: Clarient uses the DAKO method while we are using Ventana's antibody Pathway (4B5). The validation guidelines state parallel by identical method in another lab with the same validated assay is also acceptable. Can I assume that this means comparison with another lab for IHC HER2 testing and does not have to be using the same clone (DAKO vs Ventana)? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Validation of Her2
What did you do to evaluate your system and algorithms. Also the cases of ER and PR. Negative vs Postive concordance. Sent from my iPad On Mar 19, 2013, at 4:57 PM, Vickroy, Jim vickroy@mhsil.com wrote: We are working on validating HER 2 testing in our laboratory. I see that the guidelines state that we should use 25 - 100 cases to complete our validation. In the past we sent our Her2 IHC testing to Clarient and they performed the technical testing and then our pathologists would do the scoring. So for validation we used 25 of the cases we sent to Clarient and then did them in house to compare. Our comparison was nearly 100%. Here is my question: Clarient uses the DAKO method while we are using Ventana's antibody Pathway (4B5). The validation guidelines state parallel by identical method in another lab with the same validated assay is also acceptable. Can I assume that this means comparison with another lab for IHC HER2 testing and does not have to be using the same clone (DAKO vs Ventana)? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE Stainer Leica vs Sakura
Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of HEs is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
