[Histonet] RE: IF on FFPE
Hi Netters, I've read the paper, but it's not clear what temperature they reached during the first round of HIER. They do not give the volume of the first HIER solution, only the time and wattage of the microwave treatment. And which procedure do they call first and second? It looks different in the Methods section from what they mean in the Discussion. Hanna Preiszner From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Martin, Erin [erin.mar...@ucsf.edu] Sent: Tuesday, March 19, 2013 10:32 AM To: histonet Subject: [Histonet] IF on FFPE Hi everyone, Several people have contacted me regarding the article I mentioned in my post yesterday. Here are the details: Immunofluorescence With Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens AJCP 2013 139:71-78; doi:10.1309/AJCPRZG8EXN7BAID Suozhu Shi, Qingli Cheng, Ping Zhang, Nan Wang, Ying Zheng, Xue-Yuan Bai, and Xiangmei Chen Abstract: Immunofluorescence of frozen tissue sections (IF-F) is a classic technique for renal immunopathologic examination. However, it has certain disadvantages, such as diffuse antigen distribution and few or even no glomeruli in the section. We developed a new technique of immunofluorescence staining using dual microwave retrieval in paraffin-embedded renal tissue sections (IF-DMP) and compared IF-DMP with IF-F in 406 renal biopsy samples. IF-DMP detected significantly more glomeruli than did IF-F (P .001). There was no significant difference for the specificity and sensitivity in the detection of immunoglobulins, complements, κ, and λ between IF-F and IF-DMP. Concordant observations were 98% for all immunofluorescence, complements, κ, and λ staining and 100% for immunoglobulin staining. Both techniques were completely accurate in confirming diagnoses of various glomerular diseases. IF-DMP provided clearer images of tissue structure and more precise localization of antigens, and it is a suitable alternative for traditional IF-F in clinical renal immunopathologic diagnosis. This is all foreign to me - we do IF on derm following an inherited protocol. I've never worked up any IF. If anyone has thoughts on how to apply this to skin I'd appreciate it! Thanks Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Solving the Puzzle...one piece at a Time - Texas Society for Histotechnology Symposium
All, It's not to late to sign up for the Texas Society for Histotechnology State Symposium/Convention. Our meeting will include a career day for area high school students, slide contest for the HT students and a poster session. Attendees will get a chance to visit over 30 vendors and learn new techniques from 20 workshops to choose from. The meeting will be held at: J.W. Marriott, 5150 Westheimer Road, Houston, Texas When booking a room please let the hotel know you are with the Texas Society for Histotechnology We hope to see you in Houston!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tri-State Histology Symposium
All, I am forwarding this to the histonet so that you have the opportunity to attend one of the best state conventions in the nation. Hope to see you there. Glen Dawson BS, HT(ASCP), QIHCWisconsin Histology Society President From: Mitchell Jean A [mailto:jmitch...@uwhealth.org] 2013 Tri-State Histology SymposiumDear Histonetters: You are invited to participate in the Iowa, Wisconsin and Minnesota Tri-State Spring Histology Symposium that will be held at the Hotel Julien, Dubuque, Iowa April 24-26th. There is an outstanding program of seminars and workshops that begin Wednesday afternoon April 24th and conclude at noon on Friday April 26th. Join us for education, vendor displays, socializing and histotech camaraderie as we look to a Roaring Return to Dubuque in 2013. For program, registration and vendor/exhibit information contact the following representatives: Iowa: Judi Stasko (judith.sta...@ars.usda.gov) Wisconsin: Dawn Schneider (dawn.schnei...@ministryhealth.org) Minnesota: Sheri Blair (sheribla...@netzero.net) Vendor/Exhibit: Dawn Schneider (dawn.schnei...@ministryhealth.org) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Validation of Her2
Hi Jim, The way I understand this guideline, you will need to send it to a lab that uses the same Ventana clone and instrument that you use. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.trout...@vanderbilt.edumailto:ashley.trout...@vanderbilt.edu Message: 5 Date: Tue, 19 Mar 2013 17:33:03 -0500 From: Vickroy, Jim vickroy@mhsil.commailto:vickroy@mhsil.com Subject: [Histonet] Validation of Her2 To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: bb0b9f1a8373f14fa2974e8cb24bf9cf2adfb...@mmc-mail.ad.mhsil.commailto:bb0b9f1a8373f14fa2974e8cb24bf9cf2adfb...@mmc-mail.ad.mhsil.com Content-Type: text/plain; charset=us-ascii We are working on validating HER 2 testing in our laboratory. I see that the guidelines state that we should use 25 - 100 cases to complete our validation. In the past we sent our Her2 IHC testing to Clarient and they performed the technical testing and then our pathologists would do the scoring. So for validation we used 25 of the cases we sent to Clarient and then did them in house to compare. Our comparison was nearly 100%. Here is my question: Clarient uses the DAKO method while we are using Ventana's antibody Pathway (4B5). The validation guidelines state parallel by identical method in another lab with the same validated assay is also acceptable. Can I assume that this means comparison with another lab for IHC HER2 testing and does not have to be using the same clone (DAKO vs Ventana)? Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Immunofluorescence on FFPE skin
HI Erin, we tried to do the usual IHC-protocol for immunoglobulins on fixed skin-biopsies for Pemphigus. (on Ventana Benchmark, with HIER in high pH retrieval buffer). It gave very, very overstained sections. We didn't continue this project, because usual IF is easier to fix , has worked for ever and the results are typically described in pathology-literature. But I think it could work, if you make a really high dilution of the antibodies. And in mind of the brown melanin-pigment, a red chromogen would give a better contrast. In my opinion microwave plays no role - it just has to be HIER. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Martin, Erin Gesendet: Montag, 18. März 2013 19:28 An: histonet Betreff: [Histonet] Immunofluorescence on FFPE skin Hello all, My pathologist gave me a copy of Immunofluorescence with Dual Microwave Retrieval of Paraffin-Embedded Sections in the Assessment of Human Renal Biopsy Specimens from the American Journal of Clinical Pathology. He said that the same principle should work on skin and he would like to be able to do IF on fixed tissue in addition to our usual cryostat sections. Has anyone else read the paper who might be willing to give me some basic advice to try working it out? Is a microwave necessary (paper's method uses 2 different wattage settings) or is there a way to use HIER in waterbath or pressure cooker? Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] question
Thank you for your response about the squames and we are all wearing gloves now, if one uses them then we all do is my motto, although in 50 years of cutting, this is the first time this has come up. . guess I was lucky. anyway, the same pathologists would like to know if there is a standard about wearing gloves in the histo world and if so, is it because of squames or although the tissue is fixed, is there chances of infections or something else. thank you. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glutamine Synthetase and HSP 70 IHC test availability
Hello Histonetters, One of our pathologists is requesting Glutamine Synthetase and Heat Shock Protein (HSP) 70 immunostains on a liver specimen. Our usual go to reference labs do not perform these. Are there labs out there that have these tests available? Even non-reference labs??? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] question
Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. Alan Bright On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org marilyn.a.we...@kp.org wrote: Thank you for your response about the squames and we are all wearing gloves now, if one uses them then we all do is my motto, although in 50 years of cutting, this is the first time this has come up. . guess I was lucky. anyway, the same pathologists would like to know if there is a standard about wearing gloves in the histo world and if so, is it because of squames or although the tissue is fixed, is there chances of infections or something else. thank you. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Glutamine Synthetase and HSP 70 IHC test availability
We are doing these markers, but, to be perfectly honest, I am not very impressed with their results. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax Sebree Linda A lseb...@uwhealth.org 3/20/2013 5:07 PM Hello Histonetters, One of our pathologists is requesting Glutamine Synthetase and Heat Shock Protein (HSP) 70 immunostains on a liver specimen. Our usual go to reference labs do not perform these. Are there labs out there that have these tests available? Even non-reference labs??? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Best Carmine stain
If you are looking for glycogen - so a PAS. More sensitive and a deeper magenta color than the Best Carmine. Curious, what is without water? - The Frozen section? But there's water in the cells, which is what is freezing. - The stain?? But most stains are made in water (aqueous stains). Why do you need no water? Peggy Wenk, HTL(ASCP)SLS -Original Message- From: Kiranjit Grewal Sent: Saturday, March 16, 2013 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Best Carmine stain Hello, Any tips on doing Best Carmine on Frozen sections without water. Can we use mucicarmine solution to pick up glycogen in heart muscle? Thank u, Kiran Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE Stainer Leica vs Sakura (Sophia Lin)
Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. HistoCare.com Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of HEs is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HE Stainer Leica vs Sakura
Hi Sophia, I am a big fan of the Prisma stainer. We have two of them - one for routine staining, linked to the coverslipper and the other is used for Cytology and some automated special stains. We have had them for five years with no problems other than the replacement of the door latches. Lori -- Message: 7 Date: Tue, 19 Mar 2013 19:31:49 -0700 From: Sophia Lin lins0...@gmail.com Subject: [Histonet] HE Stainer Leica vs Sakura To: histonet@lists.utsouthwestern.edu Message-ID: cajgpzdqvem0s1c1efjr7ajvzv_d2ylviplrca1i-xstekik...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of HEs is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)
I agree. The Leica stainer and Sakura are both good instruments, however I really HATE that Leica glass cover slipper and I had the same assessment as to need to baby sit. I sometimes preferred just hand cover slipping because it was less trouble, and even faster sometimes ( believe that or not). I had none of these issues with the Prisma covcr slipper, and no trouble with the user interface etc. I had the same issue with the door latch on the Prisma, but other than a quick replacement of that, it worked perfectly. Joelle Weaver MAOM, HTL (ASCP) QIHC From: cont...@histocare.com Date: Wed, 20 Mar 2013 17:49:22 -0500 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. HistoCare.com Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of HEs is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question
Maybe that is why some like the UV decontamination option? I know I prefer it. If there is aerolizing of any material or particles after that cycle, at least it whatever was trapped in the chamber would have been decontaminated when you then vaccum. I think that the UV models have their own vaccum as I recall. Plus it is MUCH faster than the old defrots, wipe out, vaccum and rinse steps and air dry steps needed to decontaminate some ( usually older) cryostats. Joelle Weaver MAOM, HTL (ASCP) QIHC From: abri...@brightinstruments.com Date: Wed, 20 Mar 2013 21:37:45 + To: marilyn.a.we...@kp.org Subject: Re: [Histonet] question CC: histonet@lists.utsouthwestern.edu Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. Alan Bright On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org marilyn.a.we...@kp.org wrote: Thank you for your response about the squames and we are all wearing gloves now, if one uses them then we all do is my motto, although in 50 years of cutting, this is the first time this has come up. . guess I was lucky. anyway, the same pathologists would like to know if there is a standard about wearing gloves in the histo world and if so, is it because of squames or although the tissue is fixed, is there chances of infections or something else. thank you. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] question
sorry for the misspelling in my previous post. Joelle Weaver MAOM, HTL (ASCP) QIHC From: joellewea...@hotmail.com To: abri...@brightinstruments.com; marilyn.a.we...@kp.org Date: Wed, 20 Mar 2013 23:38:04 + Subject: RE: [Histonet] question CC: histonet@lists.utsouthwestern.edu Maybe that is why some like the UV decontamination option? I know I prefer it. If there is aerolizing of any material or particles after that cycle, at least it whatever was trapped in the chamber would have been decontaminated when you then vaccum. I think that the UV models have their own vaccum as I recall. Plus it is MUCH faster than the old defrots, wipe out, vaccum and rinse steps and air dry steps needed to decontaminate some ( usually older) cryostats. Joelle Weaver MAOM, HTL (ASCP) QIHC From: abri...@brightinstruments.com Date: Wed, 20 Mar 2013 21:37:45 + To: marilyn.a.we...@kp.org Subject: Re: [Histonet] question CC: histonet@lists.utsouthwestern.edu Strange about the gloves when vacuuming out fresh tissue trimmings from cryostats and exhausting the air back into the lab is done. Alan Bright On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org marilyn.a.we...@kp.org wrote: Thank you for your response about the squames and we are all wearing gloves now, if one uses them then we all do is my motto, although in 50 years of cutting, this is the first time this has come up. . guess I was lucky. anyway, the same pathologists would like to know if there is a standard about wearing gloves in the histo world and if so, is it because of squames or although the tissue is fixed, is there chances of infections or something else. thank you. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin)
We have both and love them both. IF you are using tape coverslips then perhaps Sakura is your best bet. We use glass coverslips on BOTH the Leica and Sakura and find fewer problems with the Leica. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Contact HistoCare Sent: Wednesday, March 20, 2013 6:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Stainer Leica vs Sakura (Sophia Lin) Both stainers are powerhouses. The Leica has a plain menu screen with a simple interface while the Sakura has a LCD screen with detailed information about what stage the staining process a rack is along with multiple menus. The difference between the performance changes drastically when the respective coverslipper attachments become involved. The Leica is seriously no match for the Sakura in this respect. The Leica's coverslipper is its Achilles heel and requires a LOT more attention and alerts frequently, very frequently. It takes a separate rack for staining the slides at the beginning of the process and eventually transfers them to a different rack one the cover slip is complete. This one uses glass and frequently drops glass, creates bubbles, drops and breaks slides. You will have to frequently purge the system and clean the cover medium needle dropper. Once done, it only holds. Two racks of 30 slides and will alert until you remove it. You can't leave this one alone for more than 5 minutes without an alert. Seriously. The Sakura's coverslipper uses cover tape which won't need to be replaced not even remotely as soon as the glass in the Leica. Finished slides remain in a carousel at the top and can hold about 10 racks of 20 before it alerts. For high volume, the Sakura pair wins hands down. You won't lose productivity time by needing to check on this machine pair. HistoCare.com Hi, We are currently looking to switch out our linear MKII stainer for either a Leica XL autostainer or the Sakura Tissue-Tek Prisma. Any recommendations? Are quantity of HEs is increasing and we need adequate equipment to meet our workload. The incorporated oven seems excellent on both stainers. Any pros/cons would be greatly appreciated! Also, if you are currently using the stainer, does it meet your workload and what is your volume? Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica XL autostainer in California?
Hi, Thanks for the responses regarding the Leica XL and the Sakura Tissue Tek Prisma! Just wondering if anyone in Southern California (Inland Empire/Los Angeles) area has the Leica XL stainer? My tech and I would love the opportunity to see it in action and to see it before we decide which to purchase. Please let me know if anyone in the southern california area has the Leica XL and would allow us to stop by to see it! We would love to treat you to lunch for your help! Thanks! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
