RE: [Histonet] Countertop fume hoods
Dear Merissa, I have used the Labconco countertop fume hood in a few different Histology settings, both MOHS and general, and found it to be very compact, easy to change the filter, and very effective at pulling the fumes away from the user. It is also a ductless system. See http://www.labconco.com/product/fume-adsorbers/35 Sincerely, Sandy Harrison VA Histology Supervisor, Minneapolis, MN 612-467-2449 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of M.O. Sent: Thursday, April 11, 2013 7:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Countertop fume hoods Hello Histonet! I am looking into small countertop fume hoods or maybe a filter system that I can use to place tissue under that has been in formalin or decalcifier to diminish the fumes. Do you have any recommendation on what and where to look for something like this? Thank you! - Merissa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] centrifuge
Hello, I am posting this for a group of veterinary students that volunteer for the local Greyhound Rescue chapter. Just a faint hope. They are looking for a donated centrifuge (benchtop, 5 ml tubes). It is to test the blood samples of rescued dogs to check for parasites before shipping the dogs across provincial/state/national borders. This use would not be restricted to Greyhounds, but also for any other volunteer work that the many veterinary student groups do here; , treating/spay/neuter feral cats, going up North and treating sled dogs, Wild Life rescue etc. We have very active caring students here. They have canvassed the vet college here , university campus etc. but no one has a small unused centrifuge. Thanks, Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GSH update
HT review is in session now. The students are very fortunate to have Robert Lott as an instructor. I have to confess I had about a dozen raw oysters last night at SeaJays but did cold coke chasers to kill the germs. We sat outside on the veranda and the weather was perfect. Vendors are setting up and we are looking forward to the vendor reception tonight. Sent from my iPhone Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GSH update
If you're a local Histotech or Pathologist within a 2 1/2 hour drive of Jekyll Island, you still have time to catch the second workshop of the day (1:30pm EST)Laser Microtomy: The Future of Soft Hard Tissue Histology! You won't want to miss this introduction to the use of femtosecond lasers to section fresh and resin embedded tissues! Jack On Apr 12, 2013, at 10:39 AM, Zimmerman, Billie bzimm...@gru.edu wrote: HT review is in session now. The students are very fortunate to have Robert Lott as an instructor. I have to confess I had about a dozen raw oysters last night at SeaJays but did cold coke chasers to kill the germs. We sat outside on the veranda and the weather was perfect. Vendors are setting up and we are looking forward to the vendor reception tonight. Sent from my iPhone Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thioflavine S for Amyloid
Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Thioflavine S for Amyloid
It is faster but ephemeral. Fluorescence is what is seen. René J. From: Mitchell Jean A jmitch...@uwhealth.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Friday, April 12, 2013 2:20 PM Subject: [Histonet] Thioflavine S for Amyloid Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Thioflavine S for Amyloid
Jean, obvious disadvantage is you need a fluorescence microscope, but then you need polarizing filters for Congo red (not as expensive though!) I haven't used Thioflavine S but Thioflavine T. My understanding is that Thioflavine S, which is a chemically different dye, tends to fluoresce strongly but nonselectively, and can overstain the whole section. Either way, the staining is not light-fast! Sections need to be kept in the dark until examined and usually within no more than a couple of days. I used it a lot back home where it seemed to be preferred more than Congo red. It is a very simple stain to perform, and obviously can be screened quickly. Dissolving the dye in 0.1M HCl gives greater selectivity. The dye soluton has a shelf-life of a month or more if kept in the dark. It is important to counterstain the sections with Mayer's hematoxylin as this quenches any nuclear fluorescence. Elastic fibers, fibrinoid, mast cell granules and Paneth cell granules fluoresce with this method but they can be easily identified as such and ignored The method of Burns et al gives probably the most consistent results: Burns J, Pennock CA, Stoward PJ. The specificity of staining of amyloid deposits with Thioflavine T. J Pathol Bacteriol 1967; 94: 337-344 Intersting comments in Puchtler's paper: Puchtler H, Sweat Waldrop F, Meloan S. Application of thiazole dyes to amyloid under conditions of direct cotton dyeing: correlation of histochemical and chemical data. Histochemistry 1983; 77: 431-445 Ronnie Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Friday, April 12, 2013 2:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thioflavine S for Amyloid Would appreciated some feedback/input from labs using Thioflavine S staining protocol for amyloid screening. Any advantages/disadvantages to this procedure vs Congo Red? Thanks much!! Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Anti-human nuclear antibody
You may also want to try the anti-NuMA antibody from cell signaling (#3888) - (rabbit polyclonal). I have found it to be an extremely clean antibody, excellent for detecting even one or two human cells lodged in mouse tissue. Good luck! ~Ally Research Technologist Comander Lab - Ocular Genomics Institute Massachusetts Eye Ear Infirmary Lab: (617)-573-6485 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 113, Issue 10
Hi Lin, I understand the desire to reduce the amount of waste hauled off as hazardous material. The issue I had with this product is that there was very little information about the product itself in the MSDS. Technically it couldn't be determined if the neutralizer itself could be dumped down the drain, much less in the company of formalin. The next question of weather the formalin was fully neutralized or not. There isn't really a good way to prove the formalin has actually been neutralized short of testing the waste solution with tests that are likely more expensive than the waste hauler in the first place. Ultimately, the decision should be made by your Department of Environmental Protection. It would be best to share the MSDS directions for use with them and let them decide if it is a good idea before trying to use it. Amos Brooks On Thu, Apr 11, 2013 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 9 Date: Thu, 11 Apr 2013 13:04:21 + From: Bustamante, Lin lbustama...@cvm.tamu.edu Subject: [Histonet] Formalin Neutralizer To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 94b6dc15aaf2f046bf847d4c1ca9aac9a959f...@cvmmb02.cvm.tamu.edu Content-Type: text/plain; charset=us-ascii We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Formalin Neutralizer
Our safety person claims no test for formalin is accurate after the addition of sodium sulfite. I had suggested some kind of testing after I was surprised by the strength of the fumes when someone was pouring the treated formalin waste down the drain. I wish I knew more about it. On Thu, Apr 11, 2013 at 6:26 PM, Cristi Rigazio cls71...@gmail.com wrote: We have a formaldehyde test kit. It's a dip stick type test. Sent from my iPhone On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote: Can I ask how you test before dumping? Thanks Mark On Apr 11, 2013 6:21 AM, Cristi Rigazio cls71...@gmail.com wrote: We neutralize ours and have no problems with it. I am not sure how much you use, so I will say it is easiest in smaller batches as you do have to shake it up to make sure it dissolves. We purchase ours from BBC Biochemical for a very reasonable price and then test before dumping. We have doing this for four years with no issues. Thanks, Cristi Sent from my iPhone On Apr 11, 2013, at 6:04 AM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: We are looking into the option of neutralizing our formalin waste instead of having it to be picked up. If you use Formalin Neutralizer, do you have any pro/con about this product? Thank you very much. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Share room for NSH
Regarding NSH 40th Anniversary Meeting: I'm trying to cut costs to attend. Are any of my friends going to the NSH meeting that would like to share a room? If you are giving a workshop, I would be more than happy to compensate you with some sort of a stipend since you get your room paid for. Let me know. I am looking not only for workshop presenters. It would be nice to share a room with a person I know, but am willing to get to know new friends. It helps to reduce costs and get to go! Thanks, Akemi Akemi Allison-Tacha BS, HT(ASCP)HTL Director Phoenix Lab Consulting E-Mail: akemiat3...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
