Re: [Histonet] special stain reagents change
If your stains contains a guideline about how many slides can be stained with them, just follow those guidelines and I am sure you will be fine. René J. From: Sophia Lin lins0...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 14, 2013 9:47 PM Subject: [Histonet] special stain reagents change Coming from a large reference laboratory, it was crucial to change out reagents for special stains every week since our workload was so heavy. Now at a smaller GI lab, I am wondering if it is absolutely necessary to change out the reagents so frequently? We have changed about 2-3 weeks depending on the amount of slide volume (the kits say up to 300-500 slides- AMT Kits). We were changing every week in the beginning, but one week our reagents were delayed so we changed it out 2 weeks later, and have noticed that the reagents still are fresh and the staining quality is not compromised. The alcohols, blueing, and xylene is changed every day. Any thoughts? Thanks in advance! Sophia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Placentas and AP
I have been asked this set of questions and thought I would ask the experts! This is regarding sending placentas to the lab for AP evaluations: Is there an industry standard, CAP or Joint Commission requirement for sending placentas for AP evaluations? Such as send all for evaluations; send complicated ones only etc. If there are specific requirements, can you please give me the req as well? Thanks for reading and considering, Michelle _ No virus found in this message. Checked by AVG - www.avg.com Version: 2013.0.3272 / Virus Database: 3162/6245 - Release Date: 04/14/13 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Blades
I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question regarding appropriate use of histonet
Howdy Folks, As I am sure you can tell we are subscribers to histonet and appreciate the generosity of members in helping solve the occasional problem we encounter. We are in need of finding either an individual or lab in our area to perform mammal root sectioning and slide preparation for the forensic mammal aging business we operate located near Burnet, Texas in the Texas Hill Country. I would like to reach out to your subscriber base to see if anyone is interested in doing some part time work for us or know someone who might be. Please let me know if that would be an appropriate use of the site. Thanks, Henry Chidgey CoFounder See what folks are saying about us http://www.deerage.com/testimonials.htm Wildlife Analytical Laboratories www.DeerAge.com http://www.deerage.com/ PO Box 295 1303 CR 118 B Burnet, Texas 78611 (512) 756-1989 (512) 663-9476 Cell Serving Wildlife Stewards, Ranchers Hunters Worldwide Customer Service and Quality of Analysis are our Priority and Passion Facebook http://www.facebook.com/DeerAge Eagle Soybeans http://deerage.com/eagle_seed/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone, Biomaterials Medical Device Implants Histology Event In Cambridge, MA - May 4th, 2013
If you are currently working with bone, biomaterials or medical device implants, you won't want to miss this specialty histology event http://www.polysciences.com/Interactive-Histology-Forum-About/185/ sponsored by Polysciences Inc. and supported with six (6) continuing education units (CEU's) by the National Society for Histotechnology! There is still time to pre-register online http://events.r20.constantcontact.com/register/event?llr=gqebcrbaboeidk=a07e6rs5o33629b055e or onsite May 4th at the Hyatt Regency Cambridge http://www.polysciences.com/Interactive-Histology-Forum-Hotel-Travel/189/. Topics of discussion will include: A Closer Look at Fixation, Decalcification and Processing Techniques for Paraffin Embedded SpecimensPresented by Robert A. Skinner, HTL (ASCP), Director of Laboratory Resources, Center for Orthopaedic Research, University of Arkansas for Medical Sciences Histology Technique Selection and Challenges for Biomaterial and Medical Device Implanted TissuesPresented by Philip Seifert, MS, HTL (ASCP), Principal Scientist, CBSET, Inc. Acrylic Resins: A Practical Approach for Demonstrating Undermineralized Bone, Biomaterials and Medical Device ImplantsPresented by Jack L. Ratliff, BA, Owner, Ratliff Histology Consultants Chairman, Hard Tissue Committee National Society for Histotechnology The Use Of A Non-Contact Laser To Collect Thin Sections From A Variety of Soft And Hard Histological Tissues, Synthetic Bio-Resorbable Materials, and Tissues Containing Non-Resorbable Medical Device ImplantsPresented by Heiko Richter, Ph.D., Sales Product Management, Rowiak GmbH Introduction to Routine Staining and Special Staining with Plastic Resin TechniquesPresented by Valantou Grover, HT (ASCP) HTL, PA, Product Line Manager, Business Manager, Histologist at Polysciences, Inc. If you have any questions, please feel free to contact myself or Ashley Gidzinski (ashley.gidzin...@polysciences.com). We look forward to seeing you in Cambridge, MA and May the 4th be with you! Best Regards, Jack Owner/Histologist, Ratliff Histology Consultants, LLC Chairman, Hard Tissue Committee - National Society for Histotechnology ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
How do I boost the power of mucicarmine for staining goblet cells? I have opened a new lot/bottle and have used full strength but it is not staining the cells. Thanks, Karla ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glucose Oxidase blocking method
Hello, I'm looking for updated information on the Glucose Oxidase blocking method. In particular, what reagents are being used now that Sigma's G5250 is no longer available. Does anyone know of a company that has this as a kit? Thanks, Denise Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Home based slide interpretation
Does anyone know of any regulations against a Pathologist reading slides from home? If so would you be able to cite the specific regulation? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immunohistochemistry for Adipose tissue macrophages in humans
Hello All I am a clinical researcher so my questions may be somewhat naive, but I would be extremely grateful for any help. I want to look at adipose tissue macrophage polarity by immunohistochemistry for activation markers. I have the following questions: 1. How much tissue sample is required. I want to use subcutaneous and omental fat samples. 2. How should I transport and store my samples. How long can I store them? I anticipate that I would be collecting samples over 2-3 months. 3. If I do HE staining first, can I do immunohistochemistry on the same slide? Can I do successive markers on the same slide? How many markers can I use it for? Thanks Khadija ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
