Re: [Histonet] Isopentane storage-the polling is open
For almost two years, we have been using- with great success- a much safer alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a paid endorser and these are my views only , and do not reflect in any way on my employer. Our pathologists greatly prefer the results of a freezing bath over the cryostat peltier method. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Isopentane storage-the polling is open
Always in an explosion proof refrigerator for storage. The isopentane in use is in an isopentane tank (at -70C) in the frozen section area of the gross room as is the fridge Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky Sent: Tuesday, April 16, 2013 2:17 PM To: 'Paula Sicurello'; HistoNet Subject: RE: [Histonet] Isopentane storage-the polling is open We store at room temperature in a flammable cabinet. Use it to freeze muscle biopsies. The Isopentane is pre-cooled by immersing a container of isopentane in a container of Liquid Nitrogen. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 16, 2013 1:35 PM To: HistoNet Subject: [Histonet] Isopentane storage-the polling is open Hello HistoNetters, I have been having a discussion with my boss about whether or not to store the isopentane (used for freezing muscle biopsies) in the refrigerator. (An expensive, explosion proof one that I would have to buy.) Her only experience is with the isopentane stored in the refrigerator, my experiences are with storing it at room temperature. What do you all do? Room temp or refrigerated? Any and all comments are greatly appreciated. Thanks! Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura film coverslipper
Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Green stain for Fat
Does anyone knows any fat stain were the fat stains green? Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Green stain for Fat
It would have to be a stain that dissolves in fat and I do not know of any with such characteristic. If you find one, please advise everybody in the list. René J. From: Bustamante, Lin lbustama...@cvm.tamu.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, April 17, 2013 9:44 AM Subject: [Histonet] Green stain for Fat Does anyone knows any fat stain were the fat stains green? Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sakura film coverslipper
Personally I prefer film coverslipper and used the one by Sakura. Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. The other disadvantage some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of any glass coverslip. Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. René J. From: MARCELLYN A. STONE mst...@cmhlink.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Wednesday, April 17, 2013 9:37 AM Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HTL Exam
Hi, I'm new around here! I am currently a research technician but want to pursue my career into Histology since I have been doing sectioning, stainings, and embedding. I have been studying for HTL exam and will take it soon. I have discussed with Histology Core at my work and they highly recommended for me to take HTL exam instead of HT because of my degree and experience. I'm wondering if anyone recently has took the HTL exam and how did it go? Was it harder than you thought it was? What materials should I focus on the most? I have checked on ASCP website and printed out study guide. I do have Histotechnology A Self Instructional Text 3rd edition. I'm getting more nervous because I don't want to fail it at first time. Thank you, Denise Smith smit...@kids.wustl.edu The materials in this email are private and may contain Protected Health Information. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this email in error, please immediately notify the sender via telephone or return email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Home based slide interpretation
John: You are absolutely right! That is also an issue. Its seems that with every bit of additional information the case about being illegal for a pathologist to read slides from home is building up more and more strong! Very interesting. René J. From: John Spaull john.r.spa...@gsk.com To: rjbu...@yahoo.com rjbu...@yahoo.com; jmacdon...@mtsac.edu jmacdon...@mtsac.edu Sent: Wednesday, April 17, 2013 2:44 AM Subject: Home based slide interpretation The UK Human Tissues Act (2006) is also relevant I think. To work with human tissue premises have to be registered. All tissue is tracked. The Health Service may have exemptions but generally tracking requires approved couriers etc. Registration of premises does require payment of a fee. One can imagine the headlines if your home working pathologist was transporting slides themselves and say left diagnostic samples on the train. Patient confidentiality around physical notes would also be something to be considered I guess. -- Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald jmacdon...@mtsac.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1366119506.58355.yahoomail...@web163104.mail.bf1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 The only regulation about reading slides from home refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. From: Jennifer MacDonald jmacdon...@mtsac.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Regards, John. John Spaull Histology Group, Molecular and Cellular Technologies, Platform Technology and Science, GlaxoSmithKline RD, Stevenage, SG1 2NY, UK +44 (0)1438 763296 E-mail john.r.spa...@gsk.com This e-mail was sent by GlaxoSmithKline Services Unlimited (registered in England and Wales No. 1047315), which is a member of the GlaxoSmithKline group of companies. The registered address of GlaxoSmithKline Services Unlimited is 980 Great West Road, Brentford, Middlesex TW8 9GS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Home based slide interpretation
It is my understanding that whenever the reading of the slides is not performed at the testing site that they need to list that on their report and also they will need to have a CLIA license for their home or office that is offsite. Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 8:35 AM To: John Spaull; jmacdon...@mtsac.edu Cc: Histonet Subject: [Histonet] Re: Home based slide interpretation John: You are absolutely right! That is also an issue. Its seems that with every bit of additional information the case about being illegal for a pathologist to read slides from home is building up more and more strong! Very interesting. René J. From: John Spaull john.r.spa...@gsk.com To: rjbu...@yahoo.com rjbu...@yahoo.com; jmacdon...@mtsac.edu jmacdon...@mtsac.edu Sent: Wednesday, April 17, 2013 2:44 AM Subject: Home based slide interpretation The UK Human Tissues Act (2006) is also relevant I think. To work with human tissue premises have to be registered. All tissue is tracked. The Health Service may have exemptions but generally tracking requires approved couriers etc. Registration of premises does require payment of a fee. One can imagine the headlines if your home working pathologist was transporting slides themselves and say left diagnostic samples on the train. Patient confidentiality around physical notes would also be something to be considered I guess. -- Message: 9 Date: Tue, 16 Apr 2013 06:38:26 -0700 (PDT) From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Home based slide interpretation To: Jennifer MacDonald jmacdon...@mtsac.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1366119506.58355.yahoomail...@web163104.mail.bf1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 The only regulation about reading slides from home refers to cytotechnologists because they have a limit of allowed cases/day that has to be controlled?at the usual work setting, but no such regulation exists for pathologists and I really?do not think that a pathologist is limited to read slides from home. The only possible objection would be a pathologist working in the UK because, under the NHS regulations,? pathologists have norms?about the?time per type of case and its complexity.? This time/case was designed to assure that the pathologist takes enough time for the diagnosis which, according with the system design, assures quality of work, and also links work-to-remuneration rates. Under those circumstances perhaps the pathologists would have to justify how much time they have used (to be paid for) per different cases, and that would require a work based control. Fortunately such a system does not exist here (yet!). Ren? J. From: Jennifer MacDonald jmacdon...@mtsac.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, April 15, 2013 6:12 PM Subject: [Histonet] Home based slide interpretation Does anyone know of any regulations against a Pathologist reading slides from home?? If so would you be able to cite the specific regulation? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Regards, John. John Spaull Histology Group, Molecular and Cellular Technologies, Platform Technology and Science, GlaxoSmithKline RD, Stevenage, SG1 2NY, UK +44 (0)1438 763296 E-mail john.r.spa...@gsk.com This e-mail was sent by GlaxoSmithKline Services Unlimited (registered in England and Wales No. 1047315), which is a member of the GlaxoSmithKline group of companies. The registered address of GlaxoSmithKline Services Unlimited is 980 Great West Road, Brentford, Middlesex TW8 9GS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fw: Isomet 2000 Precision Saw (s)
Sent: Tuesday, April 16, 2013 7:54 AM Subject: Isomet 2000 Precision Saw (s) I am interested in 1-2 replacement saws of this OLDER model, using the load system. Must be in good working condition. Thanks! 608-262-8534. Vicki Kalscheur Department of Surgical Sciences School of Veterinary Medicine University of Wisconsin 2015 Linden Drive Madison, WI 53706-1102 Phone: 608-262-8534 FAX: 608-263-7930 kalsc...@svm.vetmed.wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sakura film coverslipper
We have had a Sakura film coverslipper since 1997. It has had three service calls, mostly in last 5 years. Those service calls were minor problems quickly fixed. When we demoed several different coverslippers, we found this one to have the least amount of tweeking involved. It has been a wonderful machine and Tech One services it. They say it is a great machine. If I were buying again; I would go straight to this. Lynn Burton Animal Disease Laboratory Galesburg, Il -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of MARCELLYN A. STONE Sent: Wednesday, April 17, 2013 8:38 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pituitary gland
Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pituitary gland
Use Mallory-Azan trichrome stain. It was specifically design for pituitary gland. You can find the procedure in any good histotechnology book. René J. From: Bustamante, Lin lbustama...@cvm.tamu.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Wednesday, April 17, 2013 11:45 AM Subject: [Histonet] Pituitary gland Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Isopentane storage...
Melanie White wrote: For almost two years, we have been using- with great success- a much safer alternative to Isopentane. It is called Novec 7000 from 3M. I am NOT a paid endorser and these are my views only , and do not reflect in any way on my employer. Our pathologists greatly prefer the results of a freezing bath over the cryostat peltier method. Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 Ditto what she said about the Novec fluid. It works just as well, without the storage hazard. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Primera slide printer
Bea, Ditto what Linda said! Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 16, 2013 1:01 PM To: Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Primera slide printer Bea, You can use need to use the clipped corner slides. The best ones are the Epic Scientific slides available from CWS, Inc or the clipped corner Tanner slides available from Mercedes Medical. I'm sure others carry them also. These are the only two slides I've found that work really well in the printer. I am very happy with my slide printer. Set up was very simple and interfacing with our LIS was very easy. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, April 16, 2013 3:56 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Primera slide printer Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Primera slide printer
Thank you guys for all the feedback! It is always greatly appreciated! Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: Lori Harris [mailto:lhar...@samhealth.org] Sent: Wednesday, April 17, 2013 9:25 AM To: Blazek, Linda; Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: RE: Primera slide printer Bea, Ditto what Linda said! Lori -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Tuesday, April 16, 2013 1:01 PM To: Bea DeBrosse-Serra; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Primera slide printer Bea, You can use need to use the clipped corner slides. The best ones are the Epic Scientific slides available from CWS, Inc or the clipped corner Tanner slides available from Mercedes Medical. I'm sure others carry them also. These are the only two slides I've found that work really well in the printer. I am very happy with my slide printer. Set up was very simple and interfacing with our LIS was very easy. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Tuesday, April 16, 2013 3:56 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Primera slide printer Hello histonetters, What kind of slides, from which vendor, do you use with the Primera slide printer? Do they need to be clipped corners? Are you happy with the slide printer? Does it have major issues? Any information would be greatly appreciated. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura film coverslipper
Marcellyn, I agree with Rene. I have always worked in Histology Labs that have used the Sakura tape coverslipper and have not had any problems. If the amount of xylene that is dispensed is adequate, you should have no problems with the coverslips peeling off over time. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 6:45 AM To: MARCELLYN A. STONE; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Sakura film coverslipper Personally I prefer film coverslipper and used the one by Sakura. Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. The other disadvantage some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of any glass coverslip. Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. René J. From: MARCELLYN A. STONE mst...@cmhlink.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Wednesday, April 17, 2013 9:37 AM Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Isopentane storage-the polling is open
I just wanted to clarify that we don't use Novec 7000 for freezing of muscle tissue. We use it for other routine frozen sections on humans.Sorry if that caused any confusion. Thanks Tim for pointing that out! Melanie S. White, MT(ASCP) Laboratory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Center (843) 777-2072 NOTICE: This e-mail message and all attachments transmitted with it may contain legally PRIVILEGED and CONFIDENTIAL information intended solely for the use of the addressee. If the reader of this message is not the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this message or its attachments is strictly prohibited. If you have received this message in error, please notify the sender immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and delete this message and all copies and backups thereof. Thank You. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opening
Currently seeking histo-tech for Dermatology office that is opening up own path lab. Lab will be opening mid May. If interested please reply with resume and current number to reach you. Justin D.Wood Executive Director Arrowhead Dermatology, PC 623-487-3003 Phone 623-487-0952 Fax CONFIDENTIALITY NOTICE: The information contained in this email and any attachments is confidential and intended solely for the use of the persons named above. If the reader of this message is not the intended recipient, or employee or agent responsible for delivering it to the intended recipient, you are requested not to read, distribute, copy or otherwise use it. If you have received this communication in error, please immediately notify the sender by telephone or email, and destroy this message and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT/HTL With MOHs
To All, ***Great opportunity in Long Beach, California. Derm Lab is looking for a certified HT or HTL with MOH's experience to manage their new laboratory. Candidate must be ASCP certified and eligible to perform gross dissection under the CLIA regulations. Responsibilities would also include the following: Maintenance/editing of policies and procedures to State/CLIA standards, leading lab through State/CLIA inspection, maintenance , QA and QC for equipment and routine histology duties, such as patient accessioning, grossing, embedding, cutting, staining to cover-slipping and troubleshooting. This is a full time position that offers a competitive rate and flexible hours. Interested applicants should contact me at your earliest convenience. Please privately in-box me your resumes/CV. BEST REGARDS, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job posting
We are currently looking for Histology Techs. Experienced professionals will have an understanding of Anatomic and Clinical laboratory services for Histology. .Strong working knowledge with performance of sampling, accessioning and grossing, tissue processing and staining ,QC , .Experience with instrument calibration and validation, Testing management .Encompassing functions toward the attainment of quality testing within the laboratory. .Performs accessioning and receipt of specimens. .Tissue preparation to include grossing, processing, embedding, cutting and staining .Confirmation with pathologist of appropriate quality control material for use in the diagnosis and treatment of disease. .Perform special stains and immunohistological procedures to include all aspects of antigen retrieval, processing and staining to include the quality control and validation .Meets all the responsibility requirements of histology technologist according to state and federal law. .Assists laboratory management in the daily operation as assigned. Minimum Requirements .BS or BA in Biological Science with intended focus. .Two years experience with histology and cytology procedures with:. routine, special stains and Immunohistochemistry procedures. (IHC). .Certifications: (ASCP) Histotechnologist (HT) or eligible Please forward your resume and contact info to justin.w...@arrowheaddermatology.com Justin D.Wood Executive Director Arrowhead Dermatology, PC 623-487-3003 Phone 623-487-0952 Fax CONFIDENTIALITY NOTICE: The information contained in this email and any attachments is confidential and intended solely for the use of the persons named above. If the reader of this message is not the intended recipient, or employee or agent responsible for delivering it to the intended recipient, you are requested not to read, distribute, copy or otherwise use it. If you have received this communication in error, please immediately notify the sender by telephone or email, and destroy this message and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AutoTech Embedder( Sakura)
Hello Dear Colleagues, We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. Anyone currenly using it and would like to share their experience is great. Thank you, -Kiran ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura film coverslipper
Marcellyn, We have never owned a film coverslipper because in Hawaii, the only medical center with a film instrument ($) had problems after 5 years. The film pulled off the slides; meaning, they wanted to go back and re-cover tens of thousands of slides, in order to stay compliant. Apparently and more shockingly, they were told the problem was simply the Humidity in our area was too high for 'film'. However, in talking with our new (and wonderful) Sakura representative, I now think it was inaccurate to blame film coverslippers, especially after Rene's and Lori's endorsement. In the right hands with quality film and the right xylene coverage, I presume it would work in all settings (it's not like we're going to coverslip slides on the beach or something, although...). But we already have the Sakura Glas g2; it's a workhorse too! We love ours. Very few problems, but a little pricey. Worth the money if your volume is high, and your time is short. Hugh UH cancer center -- Date: Wed, 17 Apr 2013 09:31:05 -0700 From: Lori Harris lhar...@samhealth.org Subject: RE: [Histonet] Sakura film coverslipper To: Rene J Buesa rjbu...@yahoo.com, MARCELLYN A. STONE mst...@cmhlink.org, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Message-ID: 450edc37e404d142af67d7314c954c8a3c799d7...@shsmailvi01.int.samhealth.net Content-Type: text/plain; charset=iso-8859-1 Marcellyn, I agree with Rene. I have always worked in Histology Labs that have used the Sakura tape coverslipper and have not had any problems. If the amount of xylene that is dispensed is adequate, you should have no problems with the coverslips peeling off over time. Lori A. Harris, HT (ASCP) Histology Section Lead GSRMC Pathology Lab 3600 NW Samaritan Drive Corvallis, OR 97330 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, April 17, 2013 6:45 AM To: MARCELLYN A. STONE; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Sakura film coverslipper Personally I prefer film coverslipper and used the one by Sakura. Film coverslipping is faster and cheaper and, as long as the amount of dispensed xylene to adhere the film to the stained section is correct, the film will not come off after time. The other disadvantage some pathologists point against film is that some contend that the photomicrographs are of less quality which is NOT true. The film thickness, flatness and transparency is comparable to that of any glass coverslip. Additionally sometimes if quite difficult to remove a glass coverslip in the even of needing to restain a section, but with film coverslip the operation is simple involving the dissolution of the film with acetone. René J. From: MARCELLYN A. STONE mst...@cmhlink.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Wednesday, April 17, 2013 9:37 AM Subject: [Histonet] Sakura film coverslipper Good morning I am looking into buying a film coverslipper from Sakura. We currently use a glass coverslipper. I would like any thoughts, pros, cons that you may have on film vs. glass. I have heard that there tends to be a problem a few years later with the film curling. Any info. will be greatly appreciated. Thanks Marcy CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for use by the designated recipients named above. They are intended solely for these recipients. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify Calvert Memorial Hospital immediately by telephone at (410)535-8282 and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pituitary gland
Some references that might be useful: Paget, G. E., Eccleston, E. (1960). Simultaneous specific demonstration of thyrotroph, gonadotroph and acidophil cells in the anterior hypophysis. Biotechnic Histochemistry, 35(3), 119-122. Phifer, R. F., Spicer, S. S., Hennigar, G. R. (1973). Histochemical reactivity and staining properties of functionally defined cell types in the human adenohypophysis. The American journal of pathology, 73(3), 569. Kerenyi, N. (1959). Congo red as a simple stain for beta cells of the hypophysis. Biotechnic Histochemistry, 34(6), 343-346. Elftman, H. (1959). Combined aldehyde-fuchsin and periodic acid-Schiff staining of the pituitary. Biotechnic Histochemistry, 34(2), 77-80. Read More: http://informahealthcare.com/doi/abs/10.3109/10520295909114648 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bustamante, Lin Sent: Thursday, 18 April 2013 1:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pituitary gland Looking for any stain that shows acidophilic and basophilic cell from pituitary gland. Thank you. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
