[Histonet] RE: IHC Technician job description
Vincent, you might want check out CLIA 88' complex testing requirement before you write that job description. Cassandra Davis cda...@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, April 19, 2013 1:08 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 113, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Fwd: [Histonet] AutoTech Embedder( Sakura) (Kiranjit Grewal) 2. New Lab In KY (Hale, Meredith) 3. email (McKenzie, Emily) 4. Calcium Stains? (McKenzie, Emily) 5. RE: Calcium Stains? (Roberta Horner) 6. Biosafety in the Mohs Laboratory (Zimmerman, Billie) 7. looking for control block of Burkitt Lymphoma (Hammel, Vicky) 8. IHC question (Shirley A. Powell) 9. VCP-2001 cassette printer question about ink (Wayne D Gill) 10. atlanta employment (Michael Bourgeois) 11. RE: RE: Pituitary gland (Tony Henwood (SCHN)) 12. RE: RE: Pituitary gland (Tony Henwood (SCHN)) 13. Re: Isopentane storage-the polling is open (Maria Mejia) 14. RE: Calcium Stains? (Tony Henwood (SCHN)) 15. 2013 CAP Inspection Success National Lab (Ian R Bernard) 16. FNA preparation (Hannen, Valerie) 17. IHC Technician job description (Vincent Rivera) -- Message: 1 Date: Thu, 18 Apr 2013 10:13:23 -0700 From: Kiranjit Grewal kira...@sbcglobal.net Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: d54d92eb-04d4-490e-b059-7504ec477...@sbcglobal.net Content-Type: text/plain; charset=us-ascii Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: From: Kiranjit Grewal kira...@sbcglobal.net Date: April 17, 2013, 2:25:36 PM PDT To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AutoTech Embedder( Sakura) Hello Dear Colleagues, We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. Anyone currently using it and would like to share their experience is great. Thank you, -Kiran ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Thu, 18 Apr 2013 17:18:02 + From: Hale, Meredith mh...@miracals.com Subject: [Histonet] New Lab In KY To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 0E828EC51C7CC445A51E53F81B64E8C72242CB@s-irv-exchmb.PathologyPartners.intranet Content-Type: text/plain; charset=us-ascii Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655
[Histonet] COX2 in Human
Hello to all! I have been doing COX2 in human breast tissue and normally it works great. Lately, however, I have been getting a lot of background and nonspecific staining even with a new lot. Does anyone might know what I am doing wrong and how I could correct this problem? Thank you and HAPPY LAB WEEK!!! Pat Pat Bell (HT-ASCP) University of Colorado, Denver Medical Oncology 12801 E 17th Ave, MS 8117 Aurora, Colorado 80045 303-724-6077 pat.b...@ucdenver.edumailto:pat.b...@ucdenver.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!!
Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldnt be an email from me if I didnt tell you about my current openings. Please take a second and check them out Here Are My Spotlight Opportunities: Night Shift Histotech Patterson, NJ Brand New Lab!! Day Shift Histotech Salem, VA beautiful area and great Team HT/HTL Mohs Histotech Long Beach, CA Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staining near pap pen only????
Hello Histonetters! We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as if the tray was crooked) but both ends and NOT the middle. Can anyone think of what would cause this? My boss came up with the idea that the solution is evaporating and therefore, it's more concentrated on the ends, but that seems a stretch. Then again, it's better than what I came up with, which is nothing! Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!!
- We are celebrating with sponsor breakfasts and lunches for lab personnel from local restaurants each day. - Different internal games. - Educational Lab Week information and mementos for patients and staff alike - And finally, two half days this week, some personnel will go hiking and others on another day horseback riding. That way our mission is staffed without negative impact to patient care V/r Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pam Barker Sent: Monday, April 22, 2013 11:49 AM To: Histonet Subject: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!! Hi Histonetters. Happy Lab Week!! Wow this year is just flying by!! It was just Histotechnology Professionals Day a short time ago and here it is lab week already! So here is my question. How is your facility celebrating Lab Week? I hope you are gearing up for a fun week and if you need some ideas for lab week check out Advance Magazine, they have some fun ideas. I think we both know it wouldn't be an email from me if I didn't tell you about my current openings. Please take a second and check them out. Here Are My Spotlight Opportunities: Night Shift Histotech - Patterson, NJ Brand New Lab!! Day Shift Histotech - Salem, VA beautiful area and great Team HT/HTL Mohs Histotech - Long Beach, CA - Make it your own brand new in office lab! The rest of the histology positions that I am most excited about are located in these areas: Nashville, TN Waco, TX Tyler, TX -2 sites one at a hospital and one at a private dermpath lab Atlanta, GA Charlotte, NC Staunton, VA Louisville, KY Staunton, VA Remember if you refer someone to me and I place them now or in the future You will earn a 500 dollar referral fee!! If you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. I can be reached at 866-607-3542 or rel...@earthlink.net Thanks-Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staining near pap pen only????
Sorry, I forgot to mention, this is manual staining. Also, the area between the pap pen appears to be covered evenly with solution. By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Mon, Apr 22, 2013 at 1:32 PM, Morken, Timothy timothy.mor...@ucsfmedctr.org wrote: Emily, It could be that the reagent is pooling off the ends of the tissue. Have you observed the tissue during staining to see what is happening to the liquid on the slide? Is this manual or automated? I agree it is strange, because the usual artifact you see with the pap pen is that the pen liquid covers part of the tissue and THAT part does not stain. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Monday, April 22, 2013 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staining near pap pen only Hello Histonetters! We have been doing triple staining on frozen tissue for a while. Just recently it started to stain only near the pap pen (the sections have two lines of pap pen, one where they begin and one where they end). This makes no sense to me, as the staining isn't only on one end (as if the tray was crooked) but both ends and NOT the middle. Can anyone think of what would cause this? My boss came up with the idea that the solution is evaporating and therefore, it's more concentrated on the ends, but that seems a stretch. Then again, it's better than what I came up with, which is nothing! Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these solutions is an acceptable one for us, we still do them the oldway and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi All Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems you have encountered? Thanks MJ Image by FlamingText.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these solutions is an acceptable one for us, we still do them the oldway and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi All Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems you have encountered? Thanks MJ Image by FlamingText.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips Vanessa -Original Message- From: eloner...@metrocast.net [mailto:eloner...@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by ATT -Original Message- From: Vanessa Perez vpe...@pathreflab.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these solutions is an acceptable one for us, we still do them the oldway and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi All Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems you have encountered? Thanks MJ Image by FlamingText.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
