RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra
You just need the FITC antibodies. No kit required. The protocol is under the procedure Fluorescence IHC Basically all the machine does is dispense the antibody on the slide for however long you choose. And some rinses of reaction buffer... Vanessa -Original Message- From: Truscott, Tom [mailto:ttrus...@vetmed.wsu.edu] Sent: Monday, April 22, 2013 3:52 PM To: Vanessa Perez Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi Vanessa, I've been following this line of email, since I may need to start doing fluorescence on XT. Which kit/program/protocol do you use on the XT? Can you run them without a kit? Thankyou, Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Monday, April 22, 2013 1:22 PM To: eloner...@metrocast.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Yes, well when we take the ones off of the XT we add the neg and do the rinsing steps all together. We do two rinses of reaction buffer for 5 min then final rinse with DI water...then coverslips Vanessa -Original Message- From: eloner...@metrocast.net [mailto:eloner...@metrocast.net] Sent: Monday, April 22, 2013 3:10 PM To: Vanessa Perez Subject: Re: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Vanessa, we are just getting our DIF up and running. For your neg, it doesn't go thru any step, just coverslip direct from buffer? Thanks Eileen Lonergan MGH dermatopath associates. Sent via BlackBerry by ATT -Original Message- From: Vanessa Perez vpe...@pathreflab.com Sender: histonet-boun...@lists.utsouthwestern.edu Date: Mon, 22 Apr 2013 14:40:39 To: histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We use Benchmark here with no problems. We use tonsil controls to validate it here. For the neg. we just have an extra slide sit in reaction buffer while the other slides are on the machine. We switched from manual b/c less cost and less tech time. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter Sent: Monday, April 22, 2013 2:30 PM To: Mel John del Barrio; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra We run our DIF on our old Lab Vision stainer because it is easier and cheaper. The Ultra ready to use Abs are very expensive and there is no negative control. As far as we can find out, there is no prep kit that you can use with the fluorescence protocols to make your own. Ventana's solution is to put your negative slide in a coplin jar filled with buffer and then add that to your slide tray when you are done. We have the same issue with our Bond Max stainer - you have to use a detection kit for everything on the Bond so Leica's solution is to sell you a kit really cheap where you only use one solution and throw the rest away. Very expensive. Since neither one of these solutions is an acceptable one for us, we still do them the oldway and are very happy. I would think that unless you are running a really large number of cases, even doing them by hand is preferable to running them on the Ultra. Just my opinion. Sue Sue Hunter, Supervisor Advanced Diagnostics Beaumont Health System Royal Oak MI 248-898-5146 shun...@beaumont.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mel John del Barrio Sent: Monday, April 22, 2013 12:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Direct Immunofluorescence on Benchmark XT/Ultra Hi All Anyone in the group utilises the Benchmark for DIF's? What sort of controls do you use to validate the assay ? What problems you have encountered? Thanks MJ Image by FlamingText.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Forwarded post for a histonet member: Subject: CLIA 24-hour gross review.
I am the sole histologist in a gastroenterology laboratory situated in a GI clinic. Complying with the 24-hour grossing review by the pathologist is a challenge because my slides travel by courier across town to the pathologist to read. I receive specimens throughout the day and perform grossing in the early AM then again at 11 AM. My pathologist signs copies of the requisition that I fax over that contains the gross and sends them back to me. On a daily basis this works just fine. Fridays and holidays are a pickle but we manage. My question is the intent of the 24-hour review. The inspector cannot tell me why this was put in place, what the pathologist is supposed to do with this information and/or how to tell if it is accurate because he does not receive the slides until the following day which would be outside of the 24-hour review rule. Can anyone tell the intent of the rule? How does this rule improve patient care? Even in a hospital laboratory, once a biopsy is grossed and placed in a cassette, how on earth does looking at the written gross of how big the specimen is or what color it is impact further actions that can take place? What remedial action does CLIA expect? Thanks for your input, Gastro Gal Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Blades
I have been in histology and cutting for about 26 years now. I have used many types of blades, high and low profile. My favorite and I think the best ones are Surgipath Teflon coated high or low profile blades. I believe the high profile blades are the best but I do realize that not everyone has that option. I think if you tried them you wouldn't be disappointed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hale, Meredith Sent: Monday, April 15, 2013 12:39 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Blades I would like some feedback from you on the types of blades those of you who cut GI biopsies prefer ? Do you see differences with chatter on different blades. Any feedback is appreciated . Thanks ! Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3 cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Lab
Great opportunity for Histotechnician's in Crestview Hills, KY ! Tri-State Gastroenterology Associates is a multi-physician practice located in Northern Kentucky. Its mission is To provide compassionate, high quality, cost-effective care to patients' with gastrointestinal problems Looking for 2 Full Time HT/HTL's ( 32 hours a week is full time employment at this practice ) * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is the server down?
Haven't seen any posts over past day or so. Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glen
http://1000fungames.com/kyy.php?facebook=likeituser=safari7.16os=windows7search=lifenews ihcman2...@hotmail.com Glen ** May you be spared the indignities and infirmities of old age. . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Is the server down?
Vikki, Didn't they tell you?? You were voted off the island. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, April 23, 2013 2:10 PM To: Histo Net list server Subject: [Histonet] Is the server down? Haven't seen any posts over past day or so. Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Image analysis
Looking for opinions on digital image analyzers and software... want to do quantitative image analysis for ER/PR and HER2 IHC. Thanks! Greg Z ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recall: Image analysis
Pat Zeitlow would like to recall the message, Image analysis. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Who is scanning thieir HE's slides for Path.
We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Who is scanning thieir HE's slides for Path.
We love Aperio. http://www.aperio.com On Apr 23, 2013, at 4:02 PM, Stella Mireles estellamire...@gmail.com wrote: We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Rob Day Business Development Folio Biosciences 1476 Manning Pkwy, Powell, Ohio 43065 Direct Line: (614) 407-4547 | Main Office Phone: (614) 846-2809 | Fax: (877) 591-1815 skype: invasifspecies http://foliobio.com www.linkedin.com/in/robdaybiotech This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Who is scanning thieir HE's slides for Path.
Aperio Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, April 23, 2013 4:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Who is scanning thieir HE's slides for Path. We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Who is scanning thieir HE's slides for Path.
Aperio. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Houston, Ronald Sent: Tuesday, April 23, 2013 1:18 PM To: Stella Mireles; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who is scanning thieir HE's slides for Path. Aperio Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.org www.NationwideChildrens.org One person with passion is better than forty people merely interested. ~ E.M. Forster -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stella Mireles Sent: Tuesday, April 23, 2013 4:03 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Who is scanning thieir HE's slides for Path. We are gathering info. on the possibility of scanning our routine slides for our Path. If you are doing this in your lab, would you mind sharing some info. on the eqiupment you are using? Stella ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Image analysis
Pat There is a list of 510K cleared algorithms on the DPA website - Here is the link. https://digitalpathologyassociation.org/_data/files/DPA_Regulatory-FDA-510k_list.pdf Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Tuesday, April 23, 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Image analysis Looking for opinions on digital image analyzers and software... want to do quantitative image analysis for ER/PR and HER2 IHC. Thanks! Greg Z ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Image analysis
I'd like to add that we use the Aperio system with Ventana's antibody and it works well. It took a lot of work tweaking of the algorithm to get the software to score accurately using the 4B5 clone but that was the stain our pathologists were already used to. Mark On Tue, Apr 23, 2013 at 1:50 PM, Elizabeth Chlipala l...@premierlab.comwrote: Pat There is a list of 510K cleared algorithms on the DPA website - Here is the link. https://digitalpathologyassociation.org/_data/files/DPA_Regulatory-FDA-510k_list.pdf Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow Sent: Tuesday, April 23, 2013 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Image analysis Looking for opinions on digital image analyzers and software... want to do quantitative image analysis for ER/PR and HER2 IHC. Thanks! Greg Z ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
