Re: [Histonet] Gomori Trichrome Staining
Hi, you can try, if you do not already, to wash briefly in a solution of 0.2 ml. of glacial acetic acid in 100 ml. of distilled water, before the final dehydration. That makes the colors more delicate and transparent. The Gomori's trichromic results are similar to those obtained with the Masson's trichrome staining: Cytoplasms from bright red to greenish. Kind Regards Massimo Tosi A humble Chemical Engineer who loves Histology Da: Eleonora Guadagnin guadagnin.eleon...@gmail.com A: histonet@lists.utsouthwestern.edu Inviato: Martedì 14 Maggio 2013 21:33 Oggetto: [Histonet] Gomori Trichrome Staining Hello everybody, I am performing the Gomori Trichrome staining on frozen muscle sections (10um) but I have couple of issues: 1. The green staining is TOO dark; 2. When the green is not too dark, all my muscle fibers are red. I checked the pH of the Gomori solution, is 2.15. If anyone has some advice, please Thanks a lot! Eleonora ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mohs histology hourly rate
Good Question. I am interested in this too, as I wil lbe starting to do MOHS soon. I am located in Minnesota. On Tue, May 14, 2013 at 8:10 PM, rook...@comcast.net wrote: Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- *Jaclyn Pitts, HT(ASCP)CM* 218-454-3520 Dermatology Professionals, PA 15167 Edgewood Dr. Suite 200 Baxter, MN 56425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mohs histology hourly rate
I would expect around $25 in twin cities and between $20 and $23 outside. For MOHs with 10-15 years experience. You could find higher wages, but those would be few and far between. Sent from my iPhone On May 15, 2013, at 5:16 AM, Jaclyn Pitts pitts.jac...@gmail.com wrote: Good Question. I am interested in this too, as I wil lbe starting to do MOHS soon. I am located in Minnesota. On Tue, May 14, 2013 at 8:10 PM, rook...@comcast.net wrote: Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- *Jaclyn Pitts, HT(ASCP)CM* 218-454-3520 Dermatology Professionals, PA 15167 Edgewood Dr. Suite 200 Baxter, MN 56425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Mohs histology hourly rate
From my experience, if your new to mohs and located in the midwest (Minnesota) the typical starting wage is between $18.50-$20. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana fluorescent IHC users
Dear colleagues, If you are doing Immunofluorescence on the Ventana instruments (Alexafluor labeling), please contact me. Bonus points if you are doing them on the Discovery XT. Teri Johnson Manager, Histology GNF - San Diego, CA 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 114, Issue 14
Hi Eleanora We do trichrome on muscle but ph of solution we keep at 3.4 and first hematoxylin then trichrome for 10 minutes and then wash in water 2-3 times and then two changes of 95 percent alcohol then DCM Naveeda On 2013-05-15, at 1:02 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Lysol IC for Ventana equipmetn cleaner (Tom McNemar) 2. Re: [IHCRG] p40 (Richard Cartun) 3. RE: Gross Room cleaner (Sarah Dysart) 4. RE: Re: [IHCRG] p40 (Patsy Ruegg) 5. Bone marrow aspirate iron stain (Renz, Jason) 6. Gomori Trichrome Staining (Eleonora Guadagnin) 7. Re: Gross Room cleaner (Paula Sicurello) 8. RE: Bone marrow aspirate iron stain (Tony Henwood (SCHN)) 9. RE: Lysol IC for Ventana equipmetn cleaner (Sebree Linda A) 10. HPV In situ hybridization protocol (FISH or CISH) (Mesru T) 11. Mohs histology hourly rate (rook...@comcast.net) 12. Re: Mohs histology hourly rate (Will Chappell) 13. Re: Gomori Trichrome Staining (Massimo) 14. Re: Mohs histology hourly rate (Jaclyn Pitts) 15. Re: Mohs histology hourly rate (Will Chappell) -- Message: 1 Date: Tue, 14 May 2013 13:15:20 -0400 From: Tom McNemar tmcne...@lmhealth.org Subject: [Histonet] RE: Lysol IC for Ventana equipmetn cleaner To: 'Amber McKenzie' amber.mcken...@gastrodocs.net, Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: E9A90E28259D2F4E84308C5E8EA8F7B4014717F5E8CB@lmhs-exchange Content-Type: text/plain; charset=us-ascii We use it but we only make it up 1 gallon at a time. We use 20ml to 1 gallon of distilled per Ventana. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, May 14, 2013 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. -- Message: 2 Date: Tue, 14 May 2013 13:33:21 -0400 From: Richard Cartun rcar...@harthosp.org Subject: [Histonet] Re: [IHCRG] p40 To: 'ih...@googlegroups.com' ih...@googlegroups.com, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu, Jean Taylor jtay...@meriter.com Message-ID: 51923d2102770003a...@gwmail3.harthosp.org Content-Type: text/plain; charset=utf-8 I have been very impressed with BioCare's p40 monoclonal predilute. We are able to use it a 1:5 dilution on our Bond Max platform. Excellent immunoreactivity so far! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax Taylor, Jean
RE: [Histonet] Mohs histology hourly rate
Here in San Antonio, Texas..I hear it is a lot more than that . Maybe it is a supply and demand thing.. starting wages for a bench Histology Tech here can run between 19.00 and 21.00 dollars. I have heard that it is a lot more for a MOHS tech..like maybe around 30.00 dollars and up. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of rook...@comcast.net Sent: Tuesday, May 14, 2013 8:11 PM To: . Subject: [Histonet] Mohs histology hourly rate Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a job doing Mohs histology. Thanks in advance for any feed back, Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
