Re: [Histonet] Unsubscribe
2013/05/22 12:26 Lee Peggy Wenk lpw...@sbcglobal.net: For everyone needing to unsubscribe over the summer months while on vacation, or just because . . . keep this email handy. Go to the bottom of any email. The last line is an internet address - starts with http://has the word mailman in it. Click on the link. Scroll to the bottom of the page in the new link. type in you email address where you receive the Histonet Click on unsubscribe. Follow any other directions. Peggy Wenk -Original Message- From: Thomas Jasper Sent: Tuesday, May 21, 2013 8:28 PM To: histonet@lists.utsouthwestern.**edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Unsubscribe ___ Histonet mailing list Histonet@lists.utsouthwest... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Xylene substitutes for tissue processor clean cycle
We have two Excelsiors (had Three). We use Americlear in the cleaning cycle. We have never had issues with our cleaning cycle or the operation of our processors. Our oldest processor was 12 years old before the oven cracked and started leaking. The repair was too expensive for an older processor so we replaced it with a new one. Americlear can only be purchased through Allegiance (Baxter). -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos Sent: Tuesday, May 21, 2013 8:01 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Xylene substitutes for tissue processor clean cycle I use a excelsior and was told to always use Xylene in the cleaning cycle. Since sub is not as pure as xylene ( obviously) you could be at risk of developing paraffin clogs that the sub may not get. Using one xylene in the cleaning has not effected the odor at all in the lab. Good Luck. V.Avalos ADS, INC Fax:602-277-2134 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla Sent: Tuesday, May 21, 2013 8:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene substitutes for tissue processor clean cycle Hello all, The technician who does preventive maintenance on our LX-120 tissue processor recommended that we use xylene as the solvent in our clean cycle. We are considering using a xylene substitue in the clean cycle and would appreciate any recommendations. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for a Leica Bond or Dako Autostainer
Hi Netters, We are looking for a used Leica Bond or Dako Autostainer. If anyone out there is upgrading or has one for sale please respond to this email.. Thank you, Jill ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oven recommendations
Hi All, I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a little one since I'm in research and I don't need a big one due to the lack of volume. Does anyone have suggestions or can point me in the right direction, Please? Thanks so much and have a great day!!!Minnie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oven recommendations
Since you state you are in reasearch, consider air drying your slides. You only need to remove the water between the paraffin section and the glass slide to allow adhesion of the proteins in the tissue sample to the glass. Melting the paraffin in not necessary, your deparaffinization steps in the routine and special staining protocols will adequately remove the paraffin from the tissue section. Using an oven that was not designed to melt paraffin off the glass slide can be very hazardous. The parafin can drop don into the heating elementsand cause an ignition and fire. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: minnies...@hotmail.com To: histonet@lists.utsouthwestern.edu Date: Wed, 22 May 2013 13:55:46 + Subject: [Histonet] Oven recommendations Hi All, I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a little one since I'm in research and I don't need a big one due to the lack of volume. Does anyone have suggestions or can point me in the right direction, Please? Thanks so much and have a great day!!!Minnie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Safranin O cartilage staining
Brett Safranin O can be a bit tricky at times. There are a few tips that we have used through the years. 1. Fresh reagents are key. 2. For proteoglycan staining you can cut the sections a bit thicker and get better staining we typically cut the joint sections for Saf O at 6 to 7 microns in thickness, there are papers out there that recommend up to 8 microns in thickness. 3. Limit excess time in decal for some reason this particular stain may not work as well if the samples are in decal for an extended period of time, we have not seen this with toluidine blue which is another stain for proteoglycan. 4. We increase times in the safranin O reagent on occasion for the murine joints. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brett Tonkin Sent: Tuesday, May 21, 2013 11:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Safranin O cartilage staining Hi, We're having trouble staining murine articular cartilage with Safranin O. We first started using this stain last year and it was working nicely, with strong staining of both the articular cartilage and growth plate. After a while, the stain stopped working, with staining only visible in the growth plate. We replaced all solutions (including c/stain of fast green) and the staining worked. Yet again, it has stopped working, and this is only the second time the solutions have been used. Has anyone come across this before? Any help or advice would be greatly appreciated! Brett Tonkin Research Assistant Arthritis Research Laboratory Bone Cell Biology and Disease Unit St. Vincent's Institute Fitzroy, Victoria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Miraca Life Sciences Position's in KY
Great opportunity for Histotechnician's in Crestview Hills, KY! Miraca Life Sciences is looking to fill 2 Full Time Histologist positions in Northern Kentucky. The position will be with Miraca Life Sciences and will include benefits. The candidates must meet and have documentation to support the following requirements: * Meet CLIA Grossing Requirements : CFR 493.1489, http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens * Supervisor experience preferred * HT/HTL ASCP Certified * Experience with CLIA and CAP * Experience writing and maintaining policies and procedures * Prior laboratory start up experience is preferred * Ability to work independently Duties include: * Grossing * Embedding * Microtomy * Staining; routine and special stains only * Maintain supply orders and laboratory budget * Ability to be flexible and take on additional duties' as needed * Ability to work independently * Maintenance of laboratory for inspections * Maintenance of quality records Interested applicants should contact Meredith Hale; phone 214-596-2219 or through email mh...@miracals.commailto:mh...@miracals.com Meredith Hale HT (ASCP)cm Director External Sales Support Miraca Life Sciences 6655 North MacArthur Blvd. Irving , Texas 75039 Office: 214-596-2219 Cell: 469-648-8253 Fax: 1-866-688-3280 mh...@miracals.commailto:mh...@miracals.commailto:mh...@miracals.com%3cmailto:mh...@miracals.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Oven recommendations
Minnie This is what I have used for years as a dryer. You can go onto the TBS website : SD-II-120 Slide Dryer II, forced air, holds 2 conventional slide stainer racks, overheat protection switch, ambient to 75°C. Specifications: 100-120VAC, 50/60Hz, 2A; SD-II-220: 220-240VAC, 50/60Hz, 2A. WxDxH: (11x10x5) (26x28x13)(in)(cm). Weight: 11lbs, 5kg As far as fire goes, I do know someone that happen to and it could have been a disaster had it not been for quick thinking on the techs part. Kate Mendell Histopathology/Lab Manager HOWARD S. GOLDBERG, M.D., INC 990 Paradise Road Swampscott, MA 01907 TEL: 781.595.0151 FAX: 781.592.6780 kmend...@goldbergmd.net www.cosmesticdermcenter.com PRIVACY NOTICE: This e-mail message may contain confidential patient or other information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or authorized entity named above. The authorized recipient of this patient or other confidential information is prohibited from disclosing the information to any other party. If you have received this message in error, please notify the sender immediately and delete. Please keep any information you may have viewed confidential. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of WILLIAM DESALVO [wdesalvo@outlook.com] Sent: Wednesday, May 22, 2013 10:08 AM To: Maribel Santiago; histonet Subject: RE: [Histonet] Oven recommendations Since you state you are in reasearch, consider air drying your slides. You only need to remove the water between the paraffin section and the glass slide to allow adhesion of the proteins in the tissue sample to the glass. Melting the paraffin in not necessary, your deparaffinization steps in the routine and special staining protocols will adequately remove the paraffin from the tissue section. Using an oven that was not designed to melt paraffin off the glass slide can be very hazardous. The parafin can drop don into the heating elementsand cause an ignition and fire. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: minnies...@hotmail.com To: histonet@lists.utsouthwestern.edu Date: Wed, 22 May 2013 13:55:46 + Subject: [Histonet] Oven recommendations Hi All, I had just received a letter from Boekel (models 107800-107801 or 107905 in either 120v or 230v) that my oven can not be used to melt wax of any kind. as this is our bread and butter and need some suggestions to what kind or brand is used that is good for us (histologists). I need a little one since I'm in research and I don't need a big one due to the lack of volume. Does anyone have suggestions or can point me in the right direction, Please? Thanks so much and have a great day!!!Minnie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for mouse antibody of LTa for use in IHC
Hello Histonetters, Does anyone know of a antibody to mouse LTa (Lymphotoxin alpha (TNF Superfamily, Member 1) (LTA)) that works in IHC. A colleague is pulling her hair out trying various clones in formalin and frozen sections of mouse spleen but with no luck, please help her.. Is there one that works or is she chasing a ghost... Thanks for any help you can provide.. Jamie Jamie Erickson Abbvie laboratories Scientist II HTL (ASCP),MS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CHARLESTON, SC HISTOTECHS-I need a PRN HT
I have a PRN Ht position available for early morning fill-ins and/or set days during the week. Upcoming maternity leave also. 4:00 am until you have to go to your other job or work part time for us. GREAT crew and Pathologist to work for and opportunities to multitask, i.e., embedding, cutting, labeling, Special Stains and IHC. Position is posted on the TridentHealthSystem.com website, position #11816. Call or email me with questions. Thanks, Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Movat Pentachrome - Can't Remove Woodstain Scarlet-Acid Fuchsin from Collagen
Thank you all very much for you help. I really appreciate it. Gudrun: I tried the PTA before the WSAF and it worked beautifully (1 min PTA, dH2O, 5 min WSAF, dH20, AA, 10 min PTA). Red muscle and clean collagen.Thank-you Liz: I didn't get to trying the 60*C Bouin's but I will make a note for next time I am optimizing. Thanks again Krista On Sat, May 11, 2013 at 4:04 AM, Gudrun Lang gu.l...@gmx.at wrote: Try to stain first in PTA/PMA solution to impregnate the collagen fibers - perhaps testing with different times. Then follow up with red stain and the usual procedure. We use a stain called SFOG, that first impregnates 2 min with PMA and afterwards with the mixture of Chromotrop, Acidfuchsin and Anilinblue for 10 min. The longer we do the Polyacid-step the more intensive are the fibres and less intensive is the cytoplasma. I think, if after this trial the collagen is still red, that there are binding-sites in the collagen, that can't be influenced. Maybe acidfuchsin is here the main partner and a pure solution of Scarlet Red may help. Gudrun Lang -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Krista Sider Gesendet: Freitag, 10. Mai 2013 23:13 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Movat Pentachrome - Can’t Remove Woodstain Scarlet-Acid Fuchsin from Collagen Hello All, I have successfully stained porcine and mouse paraffin embedded heart tissues with Movat’s Pentachrome (MP), using EMS’ MP solutions and protocol (http://www.emsdiasum.com/microscopy/technical/datasheet/26385.aspx), with some optimization on times. However, now I am working with archival human tissue that has been fixed for much longer and is much older than my other samples (Human Aorta Aortic Valve, formalin fixed 2-6 months or 2-4 years, embedded in paraffin and stored at RT for ~6 years). I cannot seem to get the Woodstain Scarlet-Acid Fuchsin (WSAF) to pull out of the collagen with the 5% Phosphotungstic Acid (PA) or 1% Phosphomolybdic acid (PM) Acid. I have tried increasing the Bouin’s initial fixation (max 2 hrs 50*C), diluting the standard WSAF solution by 50% (1 dip) and taking the PA or PM up to 1 hr, yet there is always a lot of red still left in the collagen, in some regions as strong as the muscle (which I am sure are not muscle or dense cells). I see virtually no change with increased PA or PM time. As I have aorta in my samples I can’t just leave out the muscle stain. I would be very grateful for your insights into anything I could try to get clean collagen and stained muscle in the MP stain. Why might my method not be working? Is there something I can substitute for the WSAF that might be appropriate? Thank you very much for you help, Krista ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD200(OX2) that works in FFPE mouse samples
To all the histonetters, IS anyone doing CD200 staining in FFPE mouse samples with good results? If so, please share your information. Thanks in advance...your help is always appreciated!! Colleen Forster U of MN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
