RE: [Histonet] picric acid paranoia
Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. Bulk picric acid, where there is no moderator between the crystals, is another story. - Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Monday, June 03, 2013 2:30 PM To: histonet Subject: [Histonet] picric acid paranoia Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] picric acid paranoia
Ship them as you would any biological test samples. No problems here. Sent from my iPhone On Jun 4, 2013, at 5:52 AM, Smith, Allen asm...@mail.barry.edu wrote: Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric acid to the point of making it shockproof. Most of the picric acid in a fixative ends up in the hazmat bottle rather than in the tissue. Thus even putting 50 or so blocks of tissue fixed in picric acid into a hot fire would create less blast than a hearing aid battery. Bulk picric acid, where there is no moderator between the crystals, is another story. - Allen A. Smith, Ph.D. Barry University School of Podiatric Medicine -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tyrone Genade Sent: Monday, June 03, 2013 2:30 PM To: histonet Subject: [Histonet] picric acid paranoia Hello, I am moving to the USA from sunny South Africa. I would like to bring my wax blocks with me but the fish inside them were fixed with Bouin's fluid. I'm worried the picric acid could draw the wrong sort of attention. Courier companies and US Customs (which never got back to me) haven't been able to give me an answer if they are safe to travel. The blocks have sat under my lab bench for 4 years without blowing up so I guess they are perfectly safe. Anyone have an opinion on the issues or some advice on an expert (at US customs?) to contact? I would probably ship them by surface post as it just more cost effective. Thanks Tyrone Genade PhD Department of Human Biology University of Cape Town ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available?
Hi all, I have been searching for a Kupffer cell antibody. I read a paper that KCA-1 and KCA-2 are two monoclonal antibodies that are specific for Kupffer cells (PMCID: PMC1877398) . I am looking for a vendor that may have these antibodies available commercially. Alternatively, if anyone knows of other antibodies that are specific for Kupffer cells that would also be helpful! Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available?
Thanks James and Liz! I'll look into the Iba1, this is an antibody I have not looked at yet! Christina -Original Message- From: James Watson [mailto:jwat...@gnf.org] Sent: Tuesday, June 04, 2013 2:09 PM To: 'Elizabeth Chlipala'; Thurby, Christina; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? We are in the process of working up the Iba1 on mouse and it does stain the Kupffer cells, macrophages, and microglial James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, June 04, 2013 12:03 PM To: Thurby, Christina; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? Christy This is not necessarily specific for kupffer cells it will stain other macrophages but F4/80 does stain Kupffer cells. I guess I should ask which species, ED-1 will work in rat, the F4/80 in mouse and I have heard that Iba-1 stains macrophages in mice nicely that's a microglia marker, but we have not tried it on any other tissue than brain so far. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet- boun...@lists.utsouthwestern.edu] On Behalf Of Thurby, Christina Sent: Tuesday, June 04, 2013 12:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anyone know if commercial antibodies to KCA-1 and/or KCA-2 (Kupffer cell antibodies) are available? Hi all, I have been searching for a Kupffer cell antibody. I read a paper that KCA-1 and KCA-2 are two monoclonal antibodies that are specific for Kupffer cells (PMCID: PMC1877398) . I am looking for a vendor that may have these antibodies available commercially. Alternatively, if anyone knows of other antibodies that are specific for Kupffer cells that would also be helpful! Thanks! Christina Thurby Bristol Myers Squibb 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question - MDM
Is anyone using a commercially available antibody (not RUO) for the immunohistochemical detection of MDM in liposarcoma? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
