Re: [Histonet] RE: Competency for Anatomic and Clinical Pathology

2013-07-18 Thread Lee Peggy Wenk
On Wednesday (yesterday), there was a CAP teleconference on the 
to-be-updated (to be posted end of July 2013) AP checklist.


Someone asked a question about this, mentioning that someone from CAP had 
said competency assessment does not apply to histology.


The reply from the presenter and the CAP office people who could also 
respond was that competency assess does apply to histology, but that some of 
the 6 components that have to be checked for may not apply for some or most 
of the histotech jobs. So if a component of competency doesn't apply, then 
it doesn't have to be evaluated.


Below is part of the standard:

GEN.55500 Competency Assessment Phase II
The competency of each person to perform his/her assigned duties is 
assessed.
NOTE: The competency of each person to perform the duties assigned must be 
assessed following
training before the person performs patient testing.Thereafter, during the 
first year of an individual's
duties, competency must be assessed at least semiannually. After an 
individual has performed
his/her duties for one year, competency must be assessed annually. 
Retraining and reassessment
of employee competency must occur when problems are identified with employee 
performance.

Elements of competency assessment include but are not limited to:
1. Direct observations of routine patient test performance, including, as 
applicable, patient
identification and preparation; and specimen collection, handling, 
processing and testing
2. Monitoring the recording and reporting of test results, including, as 
applicable, reporting

critical results
3. Review of intermediate test results or worksheets, quality control 
records, proficiency

testing results, and preventive maintenance records
4. Direct observation of performance of instrument maintenance and function 
checks
5. Assessment of test performance through testing previously analyzed 
specimens, internal

blind testing samples or external proficiency testing samples; and
6. Evaluation of problem-solving skills

These are the 6 components, all of which must be assessed for every task 
done by histotech - except if it doesn't apply. So, for example, if you are 
assessing the competency of sectioning, then #2 - reporting of critical 
values, and #5 - blind testing samples - doesn't apply, so you don't need to 
assess via #2 and #5. But you would have to assess the person microtoming 
via the other 4 types of assessment. (But if you are participating in 
HistoQIP, microtomy is being assess via external proficiency testing sample, 
so if some of your people's slides were evaluated, you actually have #5 
covered for microtomy).


So every aspect of the histotech's job (and the lab assistant) must be 
assessed by as many of the 6 elements above as apply to each task.


CAP says we must assess competency, Joint Commission says we must assess 
competency, and CLIA says we must assess competency, and the 6 elements come 
from CLIA. So we must assess competency.


Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

The opinions expressed are mine, and do not reflect on my hospital.

-Original Message- 
From: Michelle Lamphere

Sent: Wednesday, July 17, 2013 9:10 AM
To: 'histonet@lists.utsouthwestern.edu'
Cc: Elma Cortinas
Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology

I think a few people might find this interesting

I recently attended a class about Competency Assessments in the lab.  The 
class was given by Ken Byrd (fairly certain that is how you spell his name), 
a Senior Inspector at CAP. When this particular question came up, I asked 
him to give examples of how the histology lab was supposed to use the 6 
elements to assess competency.  He informed the entire class that the 
competency assessment question with the six elements did not apply to the 
histology lab because histology did not report test results.  It is the one 
question on the Gen Lab checklist that did not apply to ANP.  Kinda 
shocking, I know.


It does not mean that we scrapped our entire competency program, we simply 
removed some of the six elements.




Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
Children's Medical Center
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamph...@childrens.com
1935 Medical District Drive | B1.06  | Dallas, Texas  75235



Message: 9
Date: Tue, 16 Jul 2013 07:14:08 -0400
From: Histology hi...@pathlab.us
Subject: [Histonet] Competency for Anatomic and Clinical Pathology at
To: histonet@lists.utsouthwestern.edu
Message-ID:
9C0B151B30AC5F4ABFD1B69D915F84CF33E6D4@dc01.DominionPath.local
Content-Type: text/plain; charset=us-ascii

Hi,



I would love to see your competency spreadsheet for histology.  We just 
finished our first CAP inspection and got a deficiency here.  He said the 
direct observation was great, but that we need to have all 6 elements.  I am 
having trouble trying to come up with a way to evaluate some of these and 
would love any help.





RE: [Histonet] RE: Competency for Anatomic and Clinical Pathology

2013-07-18 Thread joelle weaver
Thanks Peggy. I appreciate this, I hate to think we were taking a step back... 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: lpw...@sbcglobal.net
 To: michelle.lamph...@childrens.com; histonet@lists.utsouthwestern.edu
 Date: Thu, 18 Jul 2013 06:14:16 -0400
 Subject: Re: [Histonet] RE: Competency for Anatomic and Clinical Pathology
 CC: elma.corti...@childrens.com
 
 On Wednesday (yesterday), there was a CAP teleconference on the 
 to-be-updated (to be posted end of July 2013) AP checklist.
 
 Someone asked a question about this, mentioning that someone from CAP had 
 said competency assessment does not apply to histology.
 
 The reply from the presenter and the CAP office people who could also 
 respond was that competency assess does apply to histology, but that some of 
 the 6 components that have to be checked for may not apply for some or most 
 of the histotech jobs. So if a component of competency doesn't apply, then 
 it doesn't have to be evaluated.
 
 Below is part of the standard:
 
 GEN.55500 Competency Assessment Phase II
 The competency of each person to perform his/her assigned duties is 
 assessed.
 NOTE: The competency of each person to perform the duties assigned must be 
 assessed following
 training before the person performs patient testing.Thereafter, during the 
 first year of an individual's
 duties, competency must be assessed at least semiannually. After an 
 individual has performed
 his/her duties for one year, competency must be assessed annually. 
 Retraining and reassessment
 of employee competency must occur when problems are identified with employee 
 performance.
 Elements of competency assessment include but are not limited to:
 1. Direct observations of routine patient test performance, including, as 
 applicable, patient
 identification and preparation; and specimen collection, handling, 
 processing and testing
 2. Monitoring the recording and reporting of test results, including, as 
 applicable, reporting
 critical results
 3. Review of intermediate test results or worksheets, quality control 
 records, proficiency
 testing results, and preventive maintenance records
 4. Direct observation of performance of instrument maintenance and function 
 checks
 5. Assessment of test performance through testing previously analyzed 
 specimens, internal
 blind testing samples or external proficiency testing samples; and
 6. Evaluation of problem-solving skills
 
 These are the 6 components, all of which must be assessed for every task 
 done by histotech - except if it doesn't apply. So, for example, if you are 
 assessing the competency of sectioning, then #2 - reporting of critical 
 values, and #5 - blind testing samples - doesn't apply, so you don't need to 
 assess via #2 and #5. But you would have to assess the person microtoming 
 via the other 4 types of assessment. (But if you are participating in 
 HistoQIP, microtomy is being assess via external proficiency testing sample, 
 so if some of your people's slides were evaluated, you actually have #5 
 covered for microtomy).
 
 So every aspect of the histotech's job (and the lab assistant) must be 
 assessed by as many of the 6 elements above as apply to each task.
 
 CAP says we must assess competency, Joint Commission says we must assess 
 competency, and CLIA says we must assess competency, and the 6 elements come 
 from CLIA. So we must assess competency.
 
 Peggy A. Wenk, HTL(ASCP)SLS
 William Beaumont Hospital
 Royal Oak, MI 48073
 
 The opinions expressed are mine, and do not reflect on my hospital.
 
 -Original Message- 
 From: Michelle Lamphere
 Sent: Wednesday, July 17, 2013 9:10 AM
 To: 'histonet@lists.utsouthwestern.edu'
 Cc: Elma Cortinas
 Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology
 
 I think a few people might find this interesting
 
 I recently attended a class about Competency Assessments in the lab.  The 
 class was given by Ken Byrd (fairly certain that is how you spell his name), 
 a Senior Inspector at CAP. When this particular question came up, I asked 
 him to give examples of how the histology lab was supposed to use the 6 
 elements to assess competency.  He informed the entire class that the 
 competency assessment question with the six elements did not apply to the 
 histology lab because histology did not report test results.  It is the one 
 question on the Gen Lab checklist that did not apply to ANP.  Kinda 
 shocking, I know.
 
 It does not mean that we scrapped our entire competency program, we simply 
 removed some of the six elements.
 
 
 
 Michelle Lamphere
 Senior Tech, Histology
 Anatomic Pathology
 Children's Medical Center
 O: 214.456.2318 | Fax: 214.456.0779
 E: michelle.lamph...@childrens.com
 1935 Medical District Drive | B1.06  | Dallas, Texas  75235
 
 
 
 Message: 9
 Date: Tue, 16 Jul 2013 07:14:08 -0400
 From: Histology hi...@pathlab.us
 Subject: [Histonet] Competency for Anatomic and Clinical Pathology at
 To: 

[Histonet] Microtome advise...

2013-07-18 Thread Tom McNemar
Hello all.  I am preparing my budget and am seeking recommendations on 
automated microtomes.  I am specifically interested in brands other than Leica 
(I already have two).  I would appreciate any thoughts you may have.  Thanks.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org


This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
accept liability for any errors or omissions in the contents of this message, 
which arise as a result of e-mail transmission. Thank you.
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RE: [Histonet] RE: Competency for Anatomic and Clinical Pathology

2013-07-18 Thread Bernice Frederick
We do the blinded sample part. Our manager has us look at an HE stained slide 
and we identify the tissue (basic histo) and we are asked what stain is 
appropriate for the tissue (special stain or IHC)

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee  Peggy Wenk
Sent: Thursday, July 18, 2013 5:14 AM
To: Michelle Lamphere; histonet@lists.utsouthwestern.edu
Cc: Elma Cortinas
Subject: Re: [Histonet] RE: Competency for Anatomic and Clinical Pathology

On Wednesday (yesterday), there was a CAP teleconference on the to-be-updated 
(to be posted end of July 2013) AP checklist.

Someone asked a question about this, mentioning that someone from CAP had 
said competency assessment does not apply to histology.

The reply from the presenter and the CAP office people who could also respond 
was that competency assess does apply to histology, but that some of the 6 
components that have to be checked for may not apply for some or most of the 
histotech jobs. So if a component of competency doesn't apply, then it doesn't 
have to be evaluated.

Below is part of the standard:

GEN.55500 Competency Assessment Phase II The competency of each person to 
perform his/her assigned duties is assessed.
NOTE: The competency of each person to perform the duties assigned must be 
assessed following training before the person performs patient 
testing.Thereafter, during the first year of an individual's duties, competency 
must be assessed at least semiannually. After an individual has performed 
his/her duties for one year, competency must be assessed annually. 
Retraining and reassessment
of employee competency must occur when problems are identified with employee 
performance.
Elements of competency assessment include but are not limited to:
1. Direct observations of routine patient test performance, including, as 
applicable, patient identification and preparation; and specimen collection, 
handling, processing and testing 2. Monitoring the recording and reporting of 
test results, including, as applicable, reporting critical results 3. Review of 
intermediate test results or worksheets, quality control records, proficiency 
testing results, and preventive maintenance records 4. Direct observation of 
performance of instrument maintenance and function checks 5. Assessment of test 
performance through testing previously analyzed specimens, internal blind 
testing samples or external proficiency testing samples; and 6. Evaluation of 
problem-solving skills

These are the 6 components, all of which must be assessed for every task done 
by histotech - except if it doesn't apply. So, for example, if you are 
assessing the competency of sectioning, then #2 - reporting of critical values, 
and #5 - blind testing samples - doesn't apply, so you don't need to assess via 
#2 and #5. But you would have to assess the person microtoming via the other 4 
types of assessment. (But if you are participating in HistoQIP, microtomy is 
being assess via external proficiency testing sample, so if some of your 
people's slides were evaluated, you actually have #5 covered for microtomy).

So every aspect of the histotech's job (and the lab assistant) must be assessed 
by as many of the 6 elements above as apply to each task.

CAP says we must assess competency, Joint Commission says we must assess 
competency, and CLIA says we must assess competency, and the 6 elements come 
from CLIA. So we must assess competency.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

The opinions expressed are mine, and do not reflect on my hospital.

-Original Message-
From: Michelle Lamphere
Sent: Wednesday, July 17, 2013 9:10 AM
To: 'histonet@lists.utsouthwestern.edu'
Cc: Elma Cortinas
Subject: [Histonet] RE: Competency for Anatomic and Clinical Pathology

I think a few people might find this interesting

I recently attended a class about Competency Assessments in the lab.  The class 
was given by Ken Byrd (fairly certain that is how you spell his name), a Senior 
Inspector at CAP. When this particular question came up, I asked him to give 
examples of how the histology lab was supposed to use the 6 elements to assess 
competency.  He informed the entire class that the competency assessment 
question with the six elements did not apply to the histology lab because 
histology did not report test results.  It is the one question on the Gen Lab 
checklist that did not apply to ANP.  Kinda shocking, I know.

It does not mean that we scrapped our entire competency program, we simply 
removed some of the six elements.



Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
Children's Medical Center
O: 

[Histonet] Hepatocyte Nuclear Factor (HNF-1B)

2013-07-18 Thread Sebree Linda A
Good morning all,

One of our pathologists has requested this antibody and we don't have it in our 
inventory.  So I'm asking if there are any reference labs offering HNF-1B for 
FFPE human tissue?

Thanks for any/all responses.

Linda A. Sebree

University of Wisconsin Hospital  Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792

(608)265-6596
FAX: (608)262-7174


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Re: [Histonet] Microtome advise...

2013-07-18 Thread Jack Ratliff
How about the new Sakura with the auto orientation feature! I saw it on display 
at the Missouri Society for Histotechnology meeting and it was quite 
impressive! Of course if you have the budget, there's alway the non-contact 
laser microtome from Rowiak! I have seen and used that unit to cut fresh soft 
tissues (brain) and resin embedded bone and biomaterial implants!

Jack


On Jul 18, 2013, at 7:57 AM, Tom McNemar tmcne...@lmhealth.org wrote:

 Hello all.  I am preparing my budget and am seeking recommendations on 
 automated microtomes.  I am specifically interested in brands other than 
 Leica (I already have two).  I would appreciate any thoughts you may have.  
 Thanks.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you have 
 received this in error, please advise the sender by reply e-mail and delete 
 the message immediately. You may also contact the LMH Process Improvement 
 Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be 
 secure or error-free as information could be intercepted, corrupted, lost, 
 destroyed, arrive late or incomplete, or contain viruses. The sender 
 therefore does not accept liability for any errors or omissions in the 
 contents of this message, which arise as a result of e-mail transmission. 
 Thank you.
 ___
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 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 

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Re: [Histonet] Microtome advise...

2013-07-18 Thread Jack Ratliff
BTW. I'm certain that the Sakura unit will be on display at the NSH S/C meeting 
in Providence, RI (20-25 Sept 2013) and I know for a fact that the laser 
microtome will be on display there as well because it will be demonstrated and 
used, along with a Leica rotary microtome for part of a workshop I am 
co-presenting with Bob Skinner (WS # 61 - A Detailed Examination of Working 
With Decalcified and Undecalcified Bone In Support of Preclinical and Clinical 
Research).

Jack


On Jul 18, 2013, at 8:55 AM, Jack Ratliff ratliffj...@hotmail.com wrote:

 How about the new Sakura with the auto orientation feature! I saw it on 
 display at the Missouri Society for Histotechnology meeting and it was quite 
 impressive! Of course if you have the budget, there's alway the non-contact 
 laser microtome from Rowiak! I have seen and used that unit to cut fresh soft 
 tissues (brain) and resin embedded bone and biomaterial implants!
 
 Jack
 
 
 On Jul 18, 2013, at 7:57 AM, Tom McNemar tmcne...@lmhealth.org wrote:
 
 Hello all.  I am preparing my budget and am seeking recommendations on 
 automated microtomes.  I am specifically interested in brands other than 
 Leica (I already have two).  I would appreciate any thoughts you may have.  
 Thanks.
 
 Tom McNemar, HT(ASCP)
 Histology Co-ordinator
 Licking Memorial Health Systems
 (740) 348-4163
 (740) 348-4166
 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org
 www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
 
 This e-mail, including attachments, is intended for the sole use of the 
 individual and/or entity to whom it is addressed, and contains information 
 from Licking Memorial Health Systems which is confidential or privileged. If 
 you are not the intended recipient, nor authorized to receive for the 
 intended recipient, be aware that any disclosure, copying, distribution or 
 use of the contents of this e-mail and attachments is prohibited. If you 
 have received this in error, please advise the sender by reply e-mail and 
 delete the message immediately. You may also contact the LMH Process 
 Improvement Center at 740-348-4641. E-mail transmissions cannot be 
 guaranteed to be secure or error-free as information could be intercepted, 
 corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. 
 The sender therefore does not accept liability for any errors or omissions 
 in the contents of this message, which arise as a result of e-mail 
 transmission. Thank you.
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[Histonet] CLIA Compliance Regulations for histology staff coverage

2013-07-18 Thread Akemi Allison
Good morning to all in Histoland!
 
Hopefully, one of you can help me with a question which was proposed to me by a 
fellow histologist working in a very small GI histology lab. 
 
Here is what information she gave me:  She is training a histology assistant in 
house to do all the histology technical responsibilities at the request of the 
GI doctors.  She said her OJT assistant is doing a great job technically, but 
she has not yet registered in school to finish her  AA Degree so she can sit 
for the HT exam.  She has been working under her instruction for 1 year.  Her 
lab is California State and CLIA licensed.
 
The GI doctors want the assistant to cover for her while she is on vacation.  
The pathologist who is the medical director does  not think that CLIA 
Regulations allows this and wants the specimens sent out during her absence.  
She needs the CLIA Regulations stating what the guidelines are so her lab is 
complying to regulations.  She didn’t want to call CLIA because it would send a 
RED FLAG up.  I also didn’t want to call them because they may ask me what the 
name of the lab was that was proposing this question.  
 
I told her that this was most likely illegal, but she needs it in black and 
white.  Do any of you have that information or a web link to go to at your 
finger tips? She needs the regulations by next week.
 
Thank you in advance for your help,
Akemi
 
Akemi Allison-Tacha, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com
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Re: [Histonet] My perfusion problem

2013-07-18 Thread Ignacio Ruz Caracuel
Hi!

When I used to do whole body perfusion, it tooks me around 10 minutes to
perfuse with 0,9% salin and another 10 minutes with PFA. (Sorry, I haven´t
with me the rpm) but it was around 300 ml of each.

I think you have to increase the amount of 0,9% salin you perfuse (because
you have increased the circuit) to avoid clot formation.

I hope you find it useful and I wish you good luck!

Ignacio Ruz-Caracuel
Medical Student, Histology Intern Student
Faculty of Medicine,
University of Córdoba
SPAIN



2013/7/17 Leila Etemadi leila.etem...@med.lu.se

 Hi,

  I am desperately looking for an answer to my problem in perfusion
 procedure.

 What is my problem?

 To explain that I thought first I may clear what is my usual routin.

 I used to perfuse rat's brain through this procedure:

 1- Anesthetise animal with sufficient amount of pentobarbital.

 2- Clamp the vessel lying beside vertebra in the back, and then using a
 perfusion pump with an 17 or 18 gauge blunted needle inserted into the
 ascending aorta.

 3- Washout blood with 0.9% salin ( room temperature),  150-200 ml, 76 rpm
 speed ( usually I wait to see the out put solution from the heart is not
 looking so red/ blood look like).

 4- Switch to the 4% cold paraformaldehyde ( with buffer, 7.4 pH) ( on ice
 cold), 150-200 ml, 46 rpm speed. ( some times I can see the formalin dance
 but not always !).

 5- after using this amount of PFA normally the neck part is quite stiff,
 then animal will be decapitated. I extract the brain right away, postfix in
 PFA over the night ( 4°c in the refrigerator).

 6- Move to the sucrose 20%. When I saw brain is sinking in the solution (
 which normally it happens in one and half day), I freeze the brain on dry
 ice quickly.

 The results of this procedure is good enough histology after dissection
 with cryostat ( 12-20µm).



 For my recent project I need brain and spinal cord both so basically I
 skip clamping the back

 along vertebra to circulate solution through whole body ( I didn't change
 any other steps, so I do

 exactly whatever I mentioned above except for spinal cord post fixation in
 PFA is about 2 hours).

 After sectioning ( 16µm), I've got a proper tissue from spinal cord but
 when it comes to the

 brain result is quite different !!, when I replaced my sections on the
 slide ( with plus charge),

 suddenly a lot of hole began to shaped in the tissue which practically
 ruined the entire

 piece..!!!,

  First I had no clue for what I could see but then I noticed during post
 fixation procedure, when I

 transfer brain from PFA solution to the sucrose, brain is sinking right
 away ( it is not

 floating at all, as it suppose to do..) !

 So I've run another perfusion procedure but this time when I reached to
 the washing out by cold

 PFA I didn't decreased the pump speed ( as normally I decrease it from 76
 rpm to 46 rpm,

 this time I run whole procedure with the speed of 76 rpm), on the other
 hand I used more PFA

 solution ( about 350 ml PFA). After extracting brain I noticed brain was
 looking dried up.

 during post fixation and cryoprotection procedure (sucrose solution),
 sinking was looking normal,

 but after sectioning of course brain tissue was destroyed again!!!

 Now, I need your expert advices for my problem. I apologise for my naivety
 on this subject in advance.


 Humbly appreciate for your great time and attention in advance. I severely
 looking forward to your help and valuable tips.


 With the best,

 Leila









 Leila Etemadi
 M.Sc., Ph.D Candidate
 Neuronano Research Center (NRC)
 Lund University, BMC F10
 Sweden

 Tel: +46-46-2221503tel:+46-46-2221503
 E-mail: leila.etem...@med.lu.semailto:leila.etem...@med.lu.se











 Sent from my iPad

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[Histonet] RE: Histology Video

2013-07-18 Thread Sanders, Jeanine (CDC/OID/NCEZID)
Let's make one!

Actually, I probably am not allowed to...but the rest of you could!  Go for it!

Jeanine H. Sanders
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Thursday, July 18, 2013 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Video

Does anyone know of any Histology parody videos out there on YouTube?  Just 
curious!

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
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you have received this document in error.  Any further review, dissemination, 
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[Histonet] RE: Histology Video

2013-07-18 Thread Harris, Diana
Thrift Lab - UF Pathology

Diana Harris
QC  Method Development Technologist
Dept. Of Laboratory Medicine
Anatomical Pathology
Royal Jubilee Hospital - VIHA
250-370-8402



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Thursday, July 18, 2013 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Video

Does anyone know of any Histology parody videos out there on YouTube?  Just 
curious!

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
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recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
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received this communication in error, please notify us  immediately by reply 
email.  Thank you.



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[Histonet] RE: Histology Video

2013-07-18 Thread Bitting, Angela K.
I saw a few a while back. Pretty funny. It looks like they were made by some 
Pathology residents and techs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, 
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, July 18, 2013 1:02 PM
To: Heckford, Karen - SMMC-SF; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Histology Video

Let's make one!

Actually, I probably am not allowed to...but the rest of you could!  Go for it!

Jeanine H. Sanders
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Thursday, July 18, 2013 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Video

Does anyone know of any Histology parody videos out there on YouTube?  Just 
curious!

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you.



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Re: [Histonet] CLIA Compliance Regulations for histology staff coverage

2013-07-18 Thread Jennifer MacDonald
California does not have licensure for histotechs.  To my knowledge 
California does not have any regulations concerning who can cut biopsies. 
There are many labs that have uncertified people working without the 
supervision of certified people.  That being said, is this the only reason 
the Pathologist wants the cases sent our during her absence?  He is the 
one that has to sign his name to the cases.



From:   Akemi Allison akemiat3...@yahoo.com
To: Histonet Histonet@lists.utsouthwestern.edu
Date:   07/18/2013 08:34 AM
Subject:[Histonet] CLIA Compliance Regulations for histology staff 
coverage
Sent by:histonet-boun...@lists.utsouthwestern.edu



Good morning to all in Histoland!
 
Hopefully, one of you can help me with a question which was proposed to me 
by a fellow histologist working in a very small GI histology lab. 
 
Here is what information she gave me:  She is training a histology 
assistant in house to do all the histology technical responsibilities at 
the request of the GI doctors.  She said her OJT assistant is doing a 
great job technically, but she has not yet registered in school to finish 
her  AA Degree so she can sit for the HT exam.  She has been working under 
her instruction for 1 year.  Her lab is California State and CLIA 
licensed.
 
The GI doctors want the assistant to cover for her while she is on 
vacation.  The pathologist who is the medical director does  not think 
that CLIA Regulations allows this and wants the specimens sent out during 
her absence.  She needs the CLIA Regulations stating what the guidelines 
are so her lab is complying to regulations.  She didn’t want to call CLIA 
because it would send a RED FLAG up.  I also didn’t want to call them 
because they may ask me what the name of the lab was that was proposing 
this question.  
 
I told her that this was most likely illegal, but she needs it in black 
and white.  Do any of you have that information or a web link to go to at 
your finger tips? She needs the regulations by next week.
 
Thank you in advance for your help,
Akemi
 
Akemi Allison-Tacha, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com
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[Histonet] Histology Training and Competency checklist

2013-07-18 Thread Akemi Allison
Hi Again in Histoland. I have another favor to ask.  I was going to give my 
friend a Histology Training and Compentency Checklist, but I had it on my old 
computor and didn't transfer it to my new MAC.  I only have my Compentency 
checklist for Client Services from PhenoPath.  Would anyone like to share 
theirs with me? 

  
Thank you in advance for your assistance,
Akemi
 
Akemi Allison-Tacha, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com

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[Histonet] RE: Histology Video

2013-07-18 Thread Bernice Frederick
In Providence?

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen 
- SMMC-SF
Sent: Thursday, July 18, 2013 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histology Video

Does anyone know of any Histology parody videos out there on YouTube?  Just 
curious!

Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckf...@dignityhealth.org

  Caution:  This email message, including all content and attachments, is 
CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
information contained in this email message is intended only for the use of the 
recipient(s) named above. If the reader of this message is not the intended 
recipient or an agent responsible for delivering it to the intended recipient, 
you have received this document in error.  Any further review, dissemination, 
distribution, or copying of this message is strictly prohibited.  If you have 
received this communication in error, please notify us  immediately by reply 
email.  Thank you.



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[Histonet] Carnoys solution

2013-07-18 Thread Scott, Allison D
Hello to all in histoland.  Does anyone have a carnoys recipe that does not 
have call for chloroform?  I had a recipe for one, but now I can't find it.
Any and all help will be appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
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[Histonet] Histotech openings 2nd and 3rd shifts

2013-07-18 Thread Brannon Owens
ASCP certified Histotechs with IHC experience needed for a Florida
laboratory.  Two shifts available.  Email me for full job description!

Brannon Owens
Recruitment Manager
Allied Search Partners
LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823

http://www.alliedsearchpartners.com

T: 888.388.7571 ext. 106

F: 888.388.7572




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[Histonet] voice recognition

2013-07-18 Thread Hutton, Allison
We are currently working on implementing voice recognition for grossing (and 
reading in the future).  I understand how it all works but am having a hard 
time figuring it into my workflow.  If there is anyone in the eastern PA area 
that is doing this (we are using Dragon) could you please email me offline.
Thanks
Allison
ahut...@dh.org



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Re: [Histonet] RE: Histology Video

2013-07-18 Thread Cristi Rigazio
If one is organized for NSH, count me in!!

Sent from my iPhone

On Jul 18, 2013, at 11:09 AM, Bernice Frederick b-freder...@northwestern.edu 
wrote:

 In Providence?
 
 Bernice Frederick HTL (ASCP)
 Senior Research Tech
 Pathology Core Facility
 ECOGPCO-RL
 Robert. H. Lurie Cancer Center
 Northwestern University
 710 N Fairbanks Court
 Olson 8-421
 Chicago,IL 60611
 312-503-3723
 b-freder...@northwestern.edu
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heckford, 
 Karen - SMMC-SF
 Sent: Thursday, July 18, 2013 12:00 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Histology Video
 
 Does anyone know of any Histology parody videos out there on YouTube?  Just 
 curious!
 
 Karen Heckford HT ASCP CE
 Lead Histology Technician
 St. Mary's Medical Center
 450 Stanyan St.
 San Francisco, Ca. 94117
 415-668-1000 ext. 6167
 karen.heckf...@dignityhealth.org
   
Caution:  This email message, including all content and attachments, is 
 CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
 information contained in this email message is intended only for the use of 
 the recipient(s) named above. If the reader of this message is not the 
 intended recipient or an agent responsible for delivering it to the intended 
 recipient, you have received this document in error.  Any further review, 
 dissemination, distribution, or copying of this message is strictly 
 prohibited.  If you have received this communication in error, please notify 
 us  immediately by reply email.  Thank you.
 
 
 
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 Histonet@lists.utsouthwestern.edu
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[Histonet] FW: Carnoys solution

2013-07-18 Thread Clifford, Taylor


Clarke's fixative has been known to work as a replacement to Carnoy's, it is 
said to be the original Carnoy's I, whereas the Carnoy's as it is known today 
with chloroform is Carnoy's II. 

http://stainsfile.info/StainsFile/prepare/fix/fixatives/clarke.htm



Taylor CM Clifford
Research Associate
Albany Research Institute
Albany Stratton VA Medical Center
113 Holland Avenue
Albany, NY 12208
Room A610
518-626-5474
taylor.cliff...@va.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, July 18, 2013 2:25 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Carnoys solution

Hello to all in histoland.  Does anyone have a carnoys recipe that does not 
have call for chloroform?  I had a recipe for one, but now I can't find it.
Any and all help will be appreciated.



Allison Scott HT(ASCP)
Supervisor, Histology Lab
LBJ Hospital
Harris Health System
Office: 713-566-2148
Lab: 713-566-5287

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender 
by return e-mail and delete this e-mail and any attachments from your computer 
system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability and 
Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or 
Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under Texas 
law.  The e-mail is for the use of only the individual or entity named above.  
If you are not the intended recipient, or any authorized representative of the 
intended recipient, you are hereby notified that any review, dissemination or 
copying of this e-mail and its attachments is strictly prohibited.

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Re: [Histonet] CLIA Compliance Regulations for histology staff coverage

2013-07-18 Thread Kim Donadio
CLIA only regulated labs can have uncertified or licensed personel doing task 
such as embedding, microtomy and standard HE staining as CLIA does not 
regulate those task as Jennifer mentioned. That said, who is going to gross in 
the biopsies( High complexity task) in the other persons absence? Because that 
is a CLIA regulated task and needs to be done by someone who meets CLIA 1489, 
which is the education, AS degree that includes the required biology and 
chemistry, or 60 hrs of education that includes the required biology and 
chemistry. 
 
http://www.mass.gov/eohhs/docs/dph/clinical-lab/clia-lab-qualifications.pdf
 
 
Kim D 



From: Jennifer MacDonald jmacdon...@mtsac.edu
To: Akemi Allison akemiat3...@yahoo.com 
Cc: Histonet Histonet@lists.utsouthwestern.edu; 
histonet-boun...@lists.utsouthwestern.edu 
Sent: Thursday, July 18, 2013 1:27 PM
Subject: Re: [Histonet] CLIA Compliance Regulations for histology staff coverage


California does not have licensure for histotechs.  To my knowledge 
California does not have any regulations concerning who can cut biopsies. 
There are many labs that have uncertified people working without the 
supervision of certified people.  That being said, is this the only reason 
the Pathologist wants the cases sent our during her absence?  He is the 
one that has to sign his name to the cases.



From:  Akemi Allison akemiat3...@yahoo.com
To:    Histonet Histonet@lists.utsouthwestern.edu
Date:  07/18/2013 08:34 AM
Subject:        [Histonet] CLIA Compliance Regulations for histology staff 
coverage
Sent by:        histonet-boun...@lists.utsouthwestern.edu



Good morning to all in Histoland!

Hopefully, one of you can help me with a question which was proposed to me 
by a fellow histologist working in a very small GI histology lab. 

Here is what information she gave me:  She is training a histology 
assistant in house to do all the histology technical responsibilities at 
the request of the GI doctors.  She said her OJT assistant is doing a 
great job technically, but she has not yet registered in school to finish 
her  AA Degree so she can sit for the HT exam.  She has been working under 
her instruction for 1 year.  Her lab is California State and CLIA 
licensed.

The GI doctors want the assistant to cover for her while she is on 
vacation.  The pathologist who is the medical director does  not think 
that CLIA Regulations allows this and wants the specimens sent out during 
her absence.  She needs the CLIA Regulations stating what the guidelines 
are so her lab is complying to regulations.  She didn’t want to call CLIA 
because it would send a RED FLAG up.  I also didn’t want to call them 
because they may ask me what the name of the lab was that was proposing 
this question.  

I told her that this was most likely illegal, but she needs it in black 
and white.  Do any of you have that information or a web link to go to at 
your finger tips? She needs the regulations by next week.

Thank you in advance for your help,
Akemi

Akemi Allison-Tacha, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com
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[Histonet] QA forms

2013-07-18 Thread Shea's
Our state requires us to keep them for 4 years..

Before we write retention policies, we are required to look at CMS, State and 
CAP requirements. I have these attachment charts in my retention policy (as 
references). The policy states that we will retain for a minimum of  
__(most astringent). Like most, if space allows, I can keep longer, 
however, not obligatedsee attached 

Stated from CAP
Some state regulations as well as other federal mandates may require retention 
of records and/or materials for a longer time period than that specified in the 
CLIA 88 regulations; therefore any applicable state or federal laws should be 
reviewed carefully when individual laboratories develop their record retention 
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