[Histonet] Unsubscribe for NSH Symposium
For those going to the NSH Symposium, PLEASE unsubscribe, rather than having everyone on the email list read your return message of “I am out of the office” for every single Histonet email sent to you. Reminder on how: - Go to the bottom on any Histonet email - Click on the internet link (the one that starts http://) - Scroll to the bottom, fill in the information in the last “rectangle” Peggy A. Wenk, HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nail specimens
Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nail specimens
Use + charged slide and microwave prior to routine drying/melting in your slide drying oven.Also, Nair can help soften nails for cutting. Face in, coat cut surface with it and let stand for ?(1/2 hr.), wipe off Nair, chill block and cut as routine. From: casp...@yahoo.com Date: Tue, 17 Sep 2013 06:58:33 -0700 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Preventative Maintenance on reagent and formalin recyclers
I am wondering if people have a pm annually or are some stretching it out to do a pm every two years? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nail specimens
Cindy: Nails need special treatment: 1- fix the nails for 24 hours 2- place them in a 10% aq. sol. of either sodium hydroxide or potassium hydroxide until when you touch them they are flexible. Wash them in water before you touch them. Softening the keratin in the nail will take about 30-45 minutes. 3- wash them in running water until when you touch them your fingers do not have a soapy feeling. 4- process and embed as usual 5- use (+) charged slides for the sections 6- heat the sections for 5-10 nim. at 60ºC in an oven before staining. If you want I can send you a more detailed procedure. René J. From: Cindy casp...@yahoo.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 9:58 AM Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Processing Problem
Sandra Sandra We section large porcine skin samples all of the time. When there are lesions present we normally like to bisect after fixation, that way we get a nice even edge to section. You want to bisect slightly uneven (1mm) so once you trim in you are at the center of the lesion. It's very important that you try to capture sections that are at the center of the lesion, so great care must be taken in grossing, embedding and trimming in samples like these. These samples heal from the outside in so if sectioning is not consistently in the center you can bias/skew the results. Are you sure that that samples are 2mm x 2mm x 1mm or did you mean to say 2cm x 2 cm x 1cm that makes more sense to me. These samples will need to remain in fixative for at least 24 hours prior to you bisecting and trimming, after you bisect them I would continue to fix for an additional 24 to 48 hours. If the lesion is a punch it will be about 8 mm in diameter, your trimmed piece of tissue should be 2cm x 5-6mm x 1cm (LxWxD). We process these types of samples on a longer processing cycle. 1.5 to 2 hours per station with additional absolute and xylenes steps so there are 3 absolute and 3 xylenes and 4 paraffins . If they are processed well they should stay on the slide when they are sectioned when using a good plus slide. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sandra Cheasty Sent: Tuesday, September 17, 2013 9:26 AM To: histonet@lists.utsouthwestern.edu Cc: Sandra Cheasty Subject: [Histonet] Processing Problem Hi All, I need help with two issues. I have a researcher who is sending us porcine skin, about 2mm x 2mm x 1mm tall. Issue 1: There is a lesion in the center, and although he wants the skin sectioned through the lesion eventually, he says if he bisects the chunk of skin before fixation, the lesion becomes distorted. So, he is fixing them in the 2x2x1 chunks, and the 10% formalin (and subsequent processing reagents) are not penetrating. Does anyone know of either a more penetrating fixative or a less distorting one so we can bisect the skin before fixation? Issue 2: Even on smaller sections that fixed and processed well, we are having issues with the porcine skin sections staying on the slides. We use Superfrost Plus slides, drain them, air dry them overnight, and then they go on the Extra Oven program on the stainer. (25 minutes in the oven.) Any suggestions on other slides, drying techniques to try? My background is that of a certified Histologist for 30 years, with experience in many labs in various parts of the country. This research project has me stymied! Thanks for your help! Sandy UW Madison School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flammable Liquid
Is this on the CLIA website? I agree that it's difficult to find anything on there. If you're able to provide a direct link that would be great! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 17, 2013 11:56 AM To: Anne Murvosh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquid The standard is 1 gallon per every 24 square feet of lab area, so if your lab has 240 square feet surface, you can store 10 gals of flammable liquids. René J. From: Anne Murvosh amurv...@advancederm.net To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 11:23 AM Subject: [Histonet] Flammable Liquid Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations. There website is useless. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Preventative Maintenance on reagent and formalin recyclers
James We PM and calibrate annually, except for one piece of equipment - we PM the recycler every two years.We are GLP compliant and not clinical. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Laboratory Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 Work (303) 682-3949 Fax (303) 682-9060 Cell (303) 881-0763 l...@premierlab.com www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 17, 2013 8:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers I am wondering if people have a pm annually or are some stretching it out to do a pm every two years? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flammable Liquid
https://www.osha.gov/dte/library/flammable_liquids/flammable_liquids.html Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni [trathbo...@somerset-healthcare.com] Sent: Tuesday, September 17, 2013 12:14 PM To: 'Rene J Buesa'; Anne Murvosh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Flammable Liquid Is this on the CLIA website? I agree that it's difficult to find anything on there. If you're able to provide a direct link that would be great! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, September 17, 2013 11:56 AM To: Anne Murvosh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Flammable Liquid The standard is 1 gallon per every 24 square feet of lab area, so if your lab has 240 square feet surface, you can store 10 gals of flammable liquids. René J. From: Anne Murvosh amurv...@advancederm.net To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 11:23 AM Subject: [Histonet] Flammable Liquid Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations. There website is useless. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing Problem
A sample 2mmx2mmx1mm is quite small and there should not be any problems with either fixation of processing but there is where the problem my reside. 1- fixing in 10% formalin: is it neutral buffered? For such a small piece 24 hours will be enough but to be absolutely sure try leaving the pieces for 48 hours. 2- sectioning should not be a problem but I advise that you check the times in the reagents. Such small pieces should be less time in the alcohols. Since it is pig skin try using more time in the ante-medium. What are you using, xylene? And give extra time in the infiltration steps (melted paraffin wax). With these precautions you should have no problems sectioning the samples. René J. From: Sandra Cheasty cheas...@svm.vetmed.wisc.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Cc: Sandra Cheasty cheas...@svm.vetmed.wisc.edu Sent: Tuesday, September 17, 2013 11:26 AM Subject: [Histonet] Processing Problem Hi All, I need help with two issues. I have a researcher who is sending us porcine skin, about 2mm x 2mm x 1mm tall. Issue 1: There is a lesion in the center, and although he wants the skin sectioned through the lesion eventually, he says if he bisects the chunk of skin before fixation, the lesion becomes distorted. So, he is fixing them in the 2x2x1 chunks, and the 10% formalin (and subsequent processing reagents) are not penetrating. Does anyone know of either a more penetrating fixative or a less distorting one so we can bisect the skin before fixation? Issue 2: Even on smaller sections that fixed and processed well, we are having issues with the porcine skin sections staying on the slides. We use Superfrost Plus slides, drain them, air dry them overnight, and then they go on the Extra Oven program on the stainer. (25 minutes in the oven.) Any suggestions on other slides, drying techniques to try? My background is that of a certified Histologist for 30 years, with experience in many labs in various parts of the country. This research project has me stymied! Thanks for your help! Sandy UW Madison School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Flammable Liquid
The standard is 1 gallon per every 24 square feet of lab area, so if your lab has 240 square feet surface, you can store 10 gals of flammable liquids. René J. From: Anne Murvosh amurv...@advancederm.net To: histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 11:23 AM Subject: [Histonet] Flammable Liquid Can anyone tell me how many gallons of Flammable liquid can be stored in a space per CLIA regulations. There website is useless. Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Preventative Maintenance on reagent and formalin recyclers
Annually. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 17, 2013 7:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Preventative Maintenance on reagent and formalin recyclers I am wondering if people have a pm annually or are some stretching it out to do a pm every two years? Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Punch Biopsies
Regardless of the size I always bisected punch biopsies and then embedded them side-by-side resting on their flat surfaces. You have to realize that otherwise you were going to lose half the biopsy during the trimming step. Also, if the biopsy is bisected processing is facilitated. Place the skin stratum facing the blade, if the dermis is in front of the blade, the skin will be teared off. René J. From: Laurie Colbert lcolb...@pathmdlabs.com To: Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Sent: Tuesday, September 17, 2013 3:27 PM Subject: [Histonet] Punch Biopsies We are having trouble cutting punch bx's. Depending on their size, they are either left whole, bisected, or trisected. We process them on a 9 hour run with other big tissue (skin excisions, tonsils, nasal tissue, breast tissue, etc). Most of the time, the punches seemed to not be processed well - they are hard to section and we get incomplete sections. For the most part, all of the other tissue is fine to cut. Is there a trick to cutting/processing punches? We have never had so much trouble with them! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Punch Biopsies
We are having trouble cutting punch bx's. Depending on their size, they are either left whole, bisected, or trisected. We process them on a 9 hour run with other big tissue (skin excisions, tonsils, nasal tissue, breast tissue, etc). Most of the time, the punches seemed to not be processed well - they are hard to section and we get incomplete sections. For the most part, all of the other tissue is fine to cut. Is there a trick to cutting/processing punches? We have never had so much trouble with them! Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Nail specimens
From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cindy Sent: Tuesday, September 17, 2013 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Cindy, We soak faced nail blocks in 1N sodium hydroxide for about 20 mins, then section @ 5um (pathologist preference), mount on charged slides and dry @ 65°C overnight. Cut beautifully and have very few wash offs, even after DPAS staining. Good luck! Wanda Shotsberger HT/HTL Sent with AquaMail for Android http://www.aqua-mail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nail specimens
with all the answers, r u sorry u asked the question?.lol From: lowenj...@hotmail.com To: casp...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nail specimens Date: Tue, 17 Sep 2013 07:27:04 -0700 Use + charged slide and microwave prior to routine drying/melting in your slide drying oven.Also, Nair can help soften nails for cutting. Face in, coat cut surface with it and let stand for ?(1/2 hr.), wipe off Nair, chill block and cut as routine. From: casp...@yahoo.com Date: Tue, 17 Sep 2013 06:58:33 -0700 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Nail specimens
and one more thing, go with the answer from the guy who works in Dermpath at UCSF, if he doesn't know, well ? From: lowenj...@hotmail.com To: casp...@yahoo.com; histonet@lists.utsouthwestern.edu Date: Tue, 17 Sep 2013 15:33:03 -0700 Subject: RE: [Histonet] Nail specimens CC: with all the answers, r u sorry u asked the question?.lol From: lowenj...@hotmail.com To: casp...@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Nail specimens Date: Tue, 17 Sep 2013 07:27:04 -0700 Use + charged slide and microwave prior to routine drying/melting in your slide drying oven.Also, Nair can help soften nails for cutting. Face in, coat cut surface with it and let stand for ?(1/2 hr.), wipe off Nair, chill block and cut as routine. From: casp...@yahoo.com Date: Tue, 17 Sep 2013 06:58:33 -0700 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nail specimens Does anyone have a procedure for cutting nails and getting them to stay on the slide? Help please! Thanks, Cindy Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] longterm storage of tissue samples in 70% Ethanol
Dear all, I have a question about longterm storage of histology samples and was wondering if someone can help me. I am not sure if my current sample storage system is optimal in terms of antigen- and histology preservation. Do you think it is okay to store 4% paraformaldehyde fixated samples before paraffin embedding for approx 2-3 years in 70% EtOH at 4 degree celsius? Or do I risk a decrease in specimen quality? Any help will be highly appreciated. Thanks, Klaus Klaus Kruttwig Department of Cell and Tissue Biology University of California - San Francisco San Franscisco, CA, USA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gastro Tissue for Validation of Processing Protocol
Our lab is looking to acquire actual (gastric) or alternate similar tissue to validate a gastro tissue processing protocol. Any suggestions on obtaining endoscopic biopsy specimens or alternate tissue types to validate the gastric biopsy protocol? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MYC antibody
Dear People from Histonet I need to know if somebody is using Anti Myc Protein Antibody in the Panel for Large Cell Lymphomas. I will appreciate any advice on the brand of the antibody and the protocol to make it work. I do not use any Automated Stainer, only Manual Immunohistochemistry. Thank You All in advance. Sincerely yours Cesar Romero Buenos Aires Argentina Dear Cesar We are also using the Myc from Epitomics for routine diagnostic lymphoma panels on humans. Dilution is 1/50. We are running Ventana Benchmark Ultras. Happy to suggest a protocol if you are using same. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia ** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MYC antibody
Epitomics has a good rabbit mAb. We do not do this routinely. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Gary Gracie [ggra...@stvincents.com.au] Sent: Tuesday, September 17, 2013 8:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MYC antibody Dear People from Histonet I need to know if somebody is using Anti Myc Protein Antibody in the Panel for Large Cell Lymphomas. I will appreciate any advice on the brand of the antibody and the protocol to make it work. I do not use any Automated Stainer, only Manual Immunohistochemistry. Thank You All in advance. Sincerely yours Cesar Romero Buenos Aires Argentina Dear Cesar We are also using the Myc from Epitomics for routine diagnostic lymphoma panels on humans. Dilution is 1/50. We are running Ventana Benchmark Ultras. Happy to suggest a protocol if you are using same. Regards Gary Gracie Senior Technical Officer IHC Laboratory Anatomical Pathology St Vincents Hospital Sydney, Australia ** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been virus scanned and although no viruses were detected by the system, St Vincents Mater Health Sydney accepts no liability for any consequential damage resulting from email containing any computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
