[Histonet] rat's hind paw skin
Hello every body, First I would like to thank you all for your great feedbacks and attention which is such a big improvement in my work, Thank you all! Then, I was wonder if any one has experience on this tissue: rat hind paw skin ( which is hairless). Any inputs will be appreciated, whether is about any specific source where I can find technical information about fixation procedure..., or about sectioning,staining procedure… I am looking for melanin pigments and have no clue if it is possible to see it there or not?!? Thanks in advance, Cheers Leila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Labeling Error Policies
Our laboratories are currently working on policies to address specimen identification/labeling errors. This is to be a laboratory policy and will include pathology. Would anyone be willing to share policies that they have that address this and any punitive action that is mentioned? I know this has been discussed before, and continues to be a hot topic. Thanks in advance for your help regarding this. Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804) 228-6564 fax: (877)699-3895 mailto:beth@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Rate of microtome injuries in Histotechnology schools.
If anyone wants to cut down on cutting injuries. Teach the new techs to use two forceps instead of your fingers. I'm too old to change but I know a tech who cuts this way and has never had an injury. It works really well on automated microtomes. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom Wells Sent: Wednesday, November 27, 2013 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rate of microtome injuries in Histotechnology schools. Hi, I am curious about the rate of microtome cuts that students receive in Histotechnology schools. We have a rather large program and take in approximately 60 (or more) students a year. Our Histotechnology semester is around 20 weeks long. During that time our students cut approximately 300 - 400 blocks per student. We typically have approximately 10 minor cuts per semester. How does this compare to other schools? Please email or call me directly to discuss this further. Thank you very much. Tom Tom Wells BSc, MEd, MLT, ART Faculty Department of Medical Laboratory Sciences, School of Health British Columbia Institute of Technology SW03-3088, 3700 Willingdon Avenue Burnaby, BC, V5G 3H2 E tom_we...@bcit.ca T (604) 412-7594 C (778) 228 -4102 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Embedding cells in Paraffin
Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] embedding cells in parrafin #2
Making cell tissue blocks Cells: . Harvest and fix in formalin* for at least 3hrs or overnight (10 fold more formalin) . HAVE A NICE VISIBLE SIZE PELLET (1 confluent T75 minimum or 1 T175) . Remove formalin* and wash 2x's in cold 1x PBS . Cover cells with some 1x PBS and refrigerate Materials for blocks: 1. Toothpick or 20ul pipette tip 2. Agarose (2% solution agarose with 1xPBS) ultra pure agarose cat#15510-027 from core 3. Cells in 1x PBS 4. 0.5ml microtubes (sterile) 5. Tissue cassettes (1 or 6 compartiment cassettes -Electron Microscopy Science 800-523-5874 cat.# 70078-W) 6. New razor blades 7. Clean cutting board 8. Container to submerge cassette into with enough 1x PBS to cover cassettes Method: . Suspend cells in 1xPBS - use just enough to pipette cells (1:1 dilution is MAX) . Make 2% agarose solution - keep in water bath (42C) so doesn't solidify . Make 200ul agarose plug in 0.5ml tube and wait 3 minutes to solidify . Add *120ul of cell solution to .5ml tube and then add *150ul of agarose and pipette up/down ~3 times to mixed well - CUT END OF PIPETTE TIP OFF JUST A LITTLE SO YOU DON'T DESTROY CELL IF NECESSARY . Add toothpick and let sit 5 minutes . Remove plug with toothpick and cut into sections (if necessary) with new blade on clean surface and add to cassette . If you have extra cell plugs then store then in 1x PBS in frig. * Varies according to how much cell suspension you have. ALWAYS add at least 30ul more of agarose in order for it to make a nice plug. IMPORTANT NOTES: . ALWAYS be sure to label your cassette with an experiment number because that's what the slides will be labeled with. . Be SURE to write on BOTH the top of the cassette and down the sides what cell type is in each compartment (if using a 6 compartment cassette) . Tell the histologist what you fixed your cells in - if fixed in formalin, ethanol, paraformaldehyde, etc. - this is vital for the processing/embedding process . ALWAYS put control cell types in the block - +/- . ALWAYS prepare your block in an asymmetric pattern THIS IS A MUST!! or you will not be able to identify which cell type is placed where on the slide Helen L. Fedor Prostate Tissue Bank, Manager Oncology Tissue Services, Manager Johns Hopkins University 600 N. Wolfe St, | Marburg Room 406 Baltimore, MD | 21287-7065 410.614.1660 http://tmalab.jhmi.edu/ http://prostatebiorepository.org/ -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Monday, December 02, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Embedding cells in Paraffin Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Purple/Blue haze on ISH
Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller. That's the only real difference from before and after. Protocols have not changed and they've checked everything else... buffers and such. Any ideas on why this would happen? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Purple/Blue haze on ISH
Karen, We experienced a similar problem with the ISHs at our laboratory a few months ago with the Kappa, Lambda, Eber and HPV. Our pathologists were complaining of a blue crystal-like precipitate on all ISH slides. Our Ventana Technical Specialist recommended that we try the NO ALCOHOL dehydrating procedure. (The same one we currently use for the Red-Alkaline Phosphatase detection). Once you remove the slides from the Ultra or XT rinse throughly in soapy water and put in the 60 Degrees oven for 15 minutes. Dehydrate in the Symphony using a NO DEHYDRATION coverslip program. We have noticed a signficant decrease in this precipitate using this procedure. Hope if works for you too. Thank you, Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 Ph: 954-265-5317 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Bauer, Karen L. [bauer.ka...@mayo.edu] Sent: Monday, December 02, 2013 12:08 PM To: 'histonet' Subject: [Histonet] Purple/Blue haze on ISH Hi all... sending out for a colleague... After implementing the Vantage system, a lot of their ISH slides have a purplish to bluish haze on the slides. Not all of them, but some and it seems to be consistent. No problems before Vantage. They were using the longer labels from Ventana for labeling prior to implementation, but now with Vantage, the slide labels are smaller. That's the only real difference from before and after. Protocols have not changed and they've checked everything else... buffers and such. Any ideas on why this would happen? Thank you, Karen Karen L. Bauer HTL/HT (ASCP) | Histology Supervisor | Pathology | MOHS Lab Supervisor | Dermatology | Phone: 715-838-3205 | bauer.ka...@mayo.edumailto:bauer.ka...@mayo.edu | Mayo Clinic Health System | 1221 Whipple Street | Eau Claire, WI 54702 | mayoclinichealthsystem.orghttp://www.mayoclinichealthsystem.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding cells in Paraffin
I spun down the cultured cells and then used a product called histogel. It kept them protected through the VIP, paraffin embedded like cell block buttons and was able to cut regular paraffin sections. I believe that I purchased the histogel from American Mastertech. Joelle Weaver MAOM, HTL (ASCP) QIHC From: mti...@trudeauinstitute.org To: histonet@lists.utsouthwestern.edu Date: Mon, 2 Dec 2013 17:06:24 + Subject: [Histonet] Embedding cells in Paraffin Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Embedding cells in Paraffin
Mike, For EM work we fix, scrape the plate into a microtube, centrifuge at 1800 rpm for 10 min and then embed in Histogel or agar. Processing after that is as usual. They look fine by EM. I'm sure they would look fine by paraffin processing as well. Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe Sent: Monday, December 02, 2013 9:06 AM To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Embedding cells in Paraffin Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Donations Needed
Dear Histo Folks, I am in the process of setting up a brand new histology/IHC laboratory on the campus of the Uganda Cancer Institute in Kampala, Uganda in conjunction with the Fred Hutchinson Cancer Research Center. As you can imagine, we need anything and everything as supplies and small equipment are extremely hard to obtain in Uganda. If anyone is doing any end-of-year cleaning and would like to donate to a very worthy endeavor, please let me know. We are in need of everything from paraffin pots to weigh boats, beakers to vortex machines, dyes to amber bottles, and forceps to staining jars. Please don't hesitate to contact me with any questions. Thanks so much! Andrea Andrea Stritmatter, HTL (ASCP) QA Manager UCI/Hutchinson Center Cancer Alliance Fred Hutchinson Cancer Research Center 1100 Fairview Ave N, M1-B140 Seattle, WA 98109-1024 Office phone: +1-206-667-2913 Cell phone: +1-206-495-7081 Fax: +1-206-667-1965 Skype address: andrea.stritmatter Email: astri...@fhcrc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti-Human CD20
Thanks for all the great replies!! While I have you all worried about my cell pellets ;-) Does anyone have a good anti-HuCD20 that works well for FFPE tissues (cells). Thanks! Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: See you Soon
Congratulations Sarah Sincerely, Toysha N. Mayer, MBA, HT(ASCP) tnma...@mdanderson.org Instructor/Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center 713-563.3481 Message: 1 Date: Wed, 27 Nov 2013 19:13:05 + From: Sarah Dysart sdys...@mirnarx.com Subject: [Histonet] See You Soon To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 488dd7f2012c47899d4a47bfba5c9...@by2pr07mb106.namprd07.prod.outlook.com Content-Type: text/plain; charset=us-ascii Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -- Message: 2 Date: Wed, 27 Nov 2013 11:32:22 -0800 From: Heather Marlatt hmarlat...@gmail.com Subject: Re: [Histonet] See You Soon To: Sarah Dysart sdys...@mirnarx.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: calavvk6ywzb8kqxt4s+veodxcdftpwzo4vdldaq1zxp8nmp...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 CONGRATULATIONS SARAH! I hope you love the new position! :-) On Wed, Nov 27, 2013 at 11:13 AM, Sarah Dysart sdys...@mirnarx.com wrote: Hey guys and gals...I have taken a new position as Pathology Supervisor at North Austin Medical Center here in Austin...so I am unsubscribing for now, but have no fear...I will be back ASAP with my new email address. Hope everyone has a great Turkey Day!! Signing off for now... Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Grossing tables
I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Grossing tables
I think that's the best - you can charge for rides - up and down!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, December 02, 2013 4:20 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Grossing tables I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Grossing tables
http://www.thermoscientific.com/en/products/pathology-work-stations.html Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, December 02, 2013 4:24 PM To: 'Amber McKenzie'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Grossing tables I think that's the best - you can charge for rides - up and down!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Monday, December 02, 2013 4:20 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Grossing tables I'm currently designing a new GI lab. Any advice on grossing stations? The only name I know is Mopec. Is that the best one to go with or are there better ones out there? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: [IHCRG] macrophage eq markers?
In which animals works also this antibody ? Thanks On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers shive...@umn.edu wrote: Dako's MAC 387 (cat. #: M0747) On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone ferra...@vet.upenn.eduwrote: Hi All, Looking for good macrophage markers for equine tissue, FFPE IHC .? Thanks Jackie Ferracone, B.Sc. Research Specialist Dept. of Pathobiology University of Pennsylvania School of Veterinary Medicine 382 West Street Road Kennett Square, PA 19348-1692 610-925-6391 610-350-8860 (cell) 610-925-6810 (fax) -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chris van der Loos
Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: [IHCRG] macrophage eq markers?
I've had it to work in cat, cow, dog, fox, horse, otter, pig, and raccoon in my lab. According to vendor - it's supposed to also work in guinea pig, monkey, rabbit, and rat, but I have not tried it in those animals Needs either enzyme digestion or heat antigen retrieval. Best regards, Jan Shivers On Mon, Dec 2, 2013 at 3:37 PM, Mehmet Fatih BOZKURT fbozk...@gmail.comwrote: In which animals works also this antibody ? Thanks On Mon, Dec 2, 2013 at 11:20 PM, Jan Shivers shive...@umn.edu wrote: Dako's MAC 387 (cat. #: M0747) On Mon, Dec 2, 2013 at 2:09 PM, Jackie Ferracone ferra...@vet.upenn.eduwrote: Hi All, Looking for good macrophage markers for equine tissue, FFPE IHC .? Thanks Jackie Ferracone, B.Sc. Research Specialist Dept. of Pathobiology University of Pennsylvania School of Veterinary Medicine 382 West Street Road Kennett Square, PA 19348-1692 610-925-6391 610-350-8860 (cell) 610-925-6810 (fax) -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu -- -- You received this message because you are subscribed to the Google Groups ihcrg group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: histo@nsh.orgor call 443.535.4060. --- You received this message because you are subscribed to the Google Groups ihcrg group. To unsubscribe from this group and stop receiving emails from it, send an email to ihcrg+unsubscr...@googlegroups.com. For more options, visit https://groups.google.com/groups/opt_out. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Chris van der Loos
Very sad news indeed. He will be missed dearly! Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Monday, December 02, 2013 1:46 PM To: Histonet Subject: [Histonet] Chris van der Loos Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Chris van der Loos
This is very sad. Chris was knowledgeable, with a wonderful sense of humour and always had time for his colleagues. He will be missed Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Peggy Wenk Sent: Tuesday, 3 December 2013 8:46 AM To: Histonet Subject: [Histonet] Chris van der Loos Sad news. I received an email over the weekend from a histotech, that Chris van der Loos died on Nov. 26, 2013. I was able to talk with the NSH office today, and got it confirmed. There is information about Chris on the NSH webpage as of later this morning. http://www.nsh.org/content/remembering-dr-chris-van-der-loos-august-2-1955-%E2%80%93-november-26-2013 Peggy A. Wenk. HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Chris van der Loos
I had the privilege and good fortune of getting to know and swap ideas with Chris after spending a few weeks with him in Breda back in 2001. Never short of advice and always willing to help and learn from others. To use an Australian expression he was an absolute Cracker of a guy. Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases SA Pathology, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.mana...@imvs.sa.gov.au Disclaimer: Not this little black duck! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Chris van der Loos
I agree Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manavis, Jim (Health) Sent: Tuesday, 3 December 2013 10:37 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Chris van der Loos I had the privilege and good fortune of getting to know and swap ideas with Chris after spending a few weeks with him in Breda back in 2001. Never short of advice and always willing to help and learn from others. To use an Australian expression he was an absolute Cracker of a guy. Cheers Jim Jim Manavis Laboratory Manager Hanson Institute Centre for Neurological Diseases SA Pathology, Adelaide, SA, 5000 Australia Phone: 61-08-8222-3668 / 0401120697 FAX: 61-08-8222 3392 email: jim.mana...@imvs.sa.gov.au Disclaimer: Not this little black duck! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] rat's hind paw skin
Hi Leila As no one seems to have responded to your email I will add a few items. I used to do some work on wound healing on mouse footpads so circumstances are the same. If you are looking for melanin routine fixation and processing should be finem, melanin will remain. Only thing is that the epidermis is tough and there is not much dermis in this region. Need to make sure that you are careful in cutting sections or the epidermis will separate. Hope this helps if you need any more details please email me. Barry On Mon, Dec 2, 2013 at 3:44 AM, Leila Etemadi leila.etem...@med.lu.sewrote: Hello every body, First I would like to thank you all for your great feedbacks and attention which is such a big improvement in my work, Thank you all! Then, I was wonder if any one has experience on this tissue: rat hind paw skin ( which is hairless). Any inputs will be appreciated, whether is about any specific source where I can find technical information about fixation procedure..., or about sectioning,staining procedure… I am looking for melanin pigments and have no clue if it is possible to see it there or not?!? Thanks in advance, Cheers Leila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hi, There are a few ways of doing this. Perhaps the easiest is scraping the cells off while it is still in the media. Transfer it to a centrifuge tube and spin it down. Pour off the supernatant add formalism vortex it and centrifuge it again and pour off the supernatant again and add Histogel or agar. Then process as usual. This is about as close as you are going to get to treating it like normal tissue. Amos Message: 4 Date: Mon, 2 Dec 2013 17:06:24 + From: Mike Tighe mti...@trudeauinstitute.org Subject: [Histonet] Embedding cells in Paraffin To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Message-ID: 46d5589df0864870848089e6780fb...@by2pr07mb469.namprd07.prod.outlook.com Content-Type: text/plain; charset=us-ascii Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair. Thanks for any ideas!___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
