RE: [Histonet] Re: Staining on Alcohol Fixed Smears
Thanks for the clarification. I appreciate the info! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Monday, December 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Staining on Alcohol Fixed Smears I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a then, do nothing approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech -- Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemartmcne...@lmhealth.org Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox'bethc...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,levi.fr...@gmail.com levi.fr...@gmail.com Message-ID: E9A90E28259D2F4E84308C5E8EA8F7B4016951E94252@lmhs-exchange Content-Type: text/plain; charset=us-ascii Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors
RE: [Histonet] Re: Staining on Alcohol Fixed Smears
What info do you guys have on acetone fixed smears? Specifically, having trouble getting PAX5 (nuclear) to show good signal without chewing up the cells during antigen retrieval. Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, December 17, 2013 5:48 AM To: 'Beth Cox'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears Thanks for the clarification. I appreciate the info! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Monday, December 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Staining on Alcohol Fixed Smears I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a then, do nothing approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech -- Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemartmcne...@lmhealth.org Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox'bethc...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,levi.fr...@gmail.com levi.fr...@gmail.com Message-ID: E9A90E28259D2F4E84308C5E8EA8F7B4016951E94252@lmhs-exchange Content-Type: text/plain; charset=us-ascii Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended
[Histonet] Re: Staining on Alcohol Fixed Smears
I'm sorry, I have no experience with acetone fixed smears. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/17/2013 8:40 AM, Susan Foreman wrote: What info do you guys have on acetone fixed smears? Specifically, having trouble getting PAX5 (nuclear) to show good signal without chewing up the cells during antigen retrieval. Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive Knoxville, TN 37919 (865)584-1933 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, December 17, 2013 5:48 AM To: 'Beth Cox'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears Thanks for the clarification. I appreciate the info! Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Monday, December 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Staining on Alcohol Fixed Smears I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a then, do nothing approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech -- Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemartmcne...@lmhealth.org Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox'bethc...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,levi.fr...@gmail.com levi.fr...@gmail.com Message-ID: E9A90E28259D2F4E84308C5E8EA8F7B4016951E94252@lmhs-exchange Content-Type: text/plain; charset=us-ascii Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking
[Histonet] slides with flimsy coverslip coming off
We received some slides (being used in a school program) - they have flexible plastic coverslips that are coming off and the tissue is adhered to the coverslip. Does anyone have any experience or thoughts on how we might get the tissue to adhere back to the slide? Thank you! Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] slides with flimsy coverslip coming off
This can happen with the older plastic coverslipping material. I have heard that they have since fixed it though. We had several years worth of slides that this happened to, the only way we could reliably fix it was add coverslipping media below the coverslip and put something flat and heavy on top. Since the coverslips dissolve in acetone, we tried several things with attaching the tissue, using acetone to dissolve the coverslip, but it never worked very well. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 17, 2013 at 12:26 PM, Nancy Schmitt nancy_schm...@pa-ucl.comwrote: We received some slides (being used in a school program) - they have flexible plastic coverslips that are coming off and the tissue is adhered to the coverslip. Does anyone have any experience or thoughts on how we might get the tissue to adhere back to the slide? Thank you! Nancy Schmitt HT, MLT(ASCP) Histology Coordinator United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin sections or formalin fixed tissue for EM - looking for reference lab
Hi Carol, We can do that here; I am copying an internal e-mail I got from the pathologist that runs our EM. Hope this helps: Regards, Mike *** Mike, Can you follow up on this and give me some idea of projected number of cases? I would be interested in talking with Carol Wilson if you have her phone number. Thanks. Sheldon Sheldon Bastacky, MD Director, Nephropathology and Electron Microscopy Laboratory University of Pittsburgh Medical Center - Presbyterian University Hospital Department of Pathology (Room A610) 200 Lothrop Street Pittsburgh, PA 15213-2582 Phone: (412) 647-9612 Fax: (412) 647-3399 Pager: (412) 392-7205 E-mail: bastack...@upmc.edumailto:bastack...@upmc.edu https://pathconsult.upmc.com/ ** Michael A. Nalesnik, M.D. Professor of Pathology Division of Transplantation and Hepatic Pathology UPMC Montefiore Rm E738 phone 412-647-7645 fax 412-647-5237 Confidential UPMC Health System information. Any unauthorized or improper disclosure, copying, distribution, or use of the contents of this e-mail and attached document(s) is prohibited. The information contained in this e-mail and attached document(s) is intended only for the personal and confidential use of the recipient(s) named above. If you have received this communication in error, please notify the sender immediately by e-mail and delete the original e-mail and attached document(s). By communicating with UPMC staff through e-mail, you agree to comply with UPMC's e-mail terms of use found at http://www.upmc.comhttp://www.upmc.com/. Should you decide that you do not want to comply with these terms, it is your obligation to reply to those UPMC staff members with whom you are corresponding to indicate you do not agree to comply with these terms and cease further communication with UPMC by e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] grandfathered histotech
Just wondering what others have found to be the case with CAP and techs that are grandfathered in. When your labs are inspected is there a problem ? Thanks so much, hope all have a wonderful Christmas!! Anita Dudley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] grandfathered histotech
Different states with histotech licenses always provided a grand-father clause for those histotechs with years of experience lacking the theoretical training and gran-fathered them with the license at the same level to those with the studies and without much training. Consequently, a licensed HT is a licensed HT regardless of how they got their license. So the very short answer to your question is: NO! René J. From: anita azdud...@hotmail.com To: Histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, December 17, 2013 12:55 PM Subject: [Histonet] grandfathered histotech Just wondering what others have found to be the case with CAP and techs that are grandfathered in. When your labs are inspected is there a problem ? Thanks so much, hope all have a wonderful Christmas!! Anita Dudley ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job postings
Hello all, Where does everyone find the most success when advertising job openings for your histology labs? We currently use Careerbuilder, Monster and also do some advertising with NSH and a few other places. We want to make sure we're hitting the places where the most people are looking. Any thoughts are appreciated. Thanks, Lee Loss Lab Manager Dermatology Associates | Forefront Dermatology ll...@dermwisconsin.commailto:ll...@dermwisconsin.com The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New code 88343
Hello, Can someone shed some light for me on this new code for IHC? I think the code refers to double stain antibodies? Thanks in advance : ) Paula Lucas Lab Manager Bio-Path Medical Group ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: job postings
I found both of my current jobs in ADVANCE magazine. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, December 17, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] job postings Hello all, Where does everyone find the most success when advertising job openings for your histology labs? We currently use Careerbuilder, Monster and also do some advertising with NSH and a few other places. We want to make sure we're hitting the places where the most people are looking. Any thoughts are appreciated. Thanks, Lee Loss Lab Manager Dermatology Associates | Forefront Dermatology ll...@dermwisconsin.commailto:ll...@dermwisconsin.com The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: job postings
www.histosearch.com has a histology job page so that you can list or acquire jobs. Shirley Powell -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee Loss Sent: Tuesday, December 17, 2013 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] job postings Hello all, Where does everyone find the most success when advertising job openings for your histology labs? We currently use Careerbuilder, Monster and also do some advertising with NSH and a few other places. We want to make sure we're hitting the places where the most people are looking. Any thoughts are appreciated. Thanks, Lee Loss Lab Manager Dermatology Associates | Forefront Dermatology ll...@dermwisconsin.commailto:ll...@dermwisconsin.com The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] beautiful microscope photos
Just for fun. This year’s top 10 Olympus Bioscapes digital imaging competition. Most through microscopes. http://www.wired.com/wiredscience/2013/12/olympus-bioscapes-microscope-photography/#slideid-395121 Peggy A. Wenk, HTL(ASCP)SLS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Discontinuing Negative Reagent Controls for IHC
Below is the list of Histonet responses representing the labs that have discontinued the use of negative reagent controls for IHC's. Thanks to everyone who responded. Roger Maywood, IL Molecular Diagnostics Lab, Winston-Salem, NC Saint Joseph's Hospital, Atlanta, GA Hartford Hospital, Hartford, CT UC San Francisco Medical Center, San Francisco, CA UCSD Medical Center, San Diego, CA Central Vermont Medical Center, Berlin, VT Beaumont Health System, Royal Oak, MI Saint Francis Medical Center, Cape Girardeau, MO Dominion Pathology Laboratories, Norfolk, VA CSI Laboratories, Alpharetta, GA Unitypoint Health-Methodist, Peoria, IL Rutland Regional Medical Center, Rutland, VT Vanderbilt University, Nashville, TN Physicians Reference Laboratory, LLC, Overland Park, KS Regions Hospital, St. Paul, MN United Clinical Laboratories, Dubuque, IA Holy Redeemer Hospital Laboratory, Meadowbrook, PA St. Luke's Hospital, Duluth, MN PRHS, Naples, FL Roger Heyna rhe...@lumc.edu 12/9/2013 2:47 PM I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
