[Histonet] Re: Histogel
Hi Jennifer, We use low melt point agarose to pre-embed and it works consistently well with our processing. Material: * 10% Neutral Buffered Formalin * 1X Dulbeccos PBS * NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080 Solutions: 1% Neutral Buffered Formalin 10% Neutral Buffered Formalin...10 ml 1X DPBS...90 ml 3.0% Agarose NuSieve Low Melting Point Agarose powder..3.0 gm 1% Neutral Buffer Formalin...100.0 ml 1.In a fume hood, warm up the mixture until the agarose completely dissolves and maintain the solution at approximately 40 degrees C. 2.Aliquot into tubes and store at room temperature. 3.Prior to use, warm the agarose to 40 degrees C until completely melted. Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thermo Scientific Embedding Centre Histostar
We are looking at purchasing a Histostar and would like to know if you have had any trouble with the touch screen? I had to replace the touch screen on our Histocenter 3 because we could no longer navigate the menu with all the lines going thru it. Wanted to know if it was just our bad luck or if anyone else has had the same experience with the touch screen. Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue-Tek TEC5
We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tissue-Tek TEC5
No. I have had 3 Tissue Tek embedding stations and never felt it was cold enough. It is in such close proximity to all that heat that I just assume that it is what it is. Other than that, no problems. I have found them to be very reliable. Tom McNemar, HT(ASCP) Histology Supervisor Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 1:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue-Tek TEC5 We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Thermo Scientific Embedding Centre Histostar
I have the Histostar embedding center (purchased 9/2012) and have not had any problems with the touch screen. Lynne Bell, HT (ASCP) Histology Team Leader Central Vermont Medical Center 130 Fisher Road Berlin, VT 05641 802-371-4923 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 1:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Thermo Scientific Embedding Centre Histostar We are looking at purchasing a Histostar and would like to know if you have had any trouble with the touch screen? I had to replace the touch screen on our Histocenter 3 because we could no longer navigate the menu with all the lines going thru it. Wanted to know if it was just our bad luck or if anyone else has had the same experience with the touch screen. Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tissue-Tek TEC5
It doesn't seem cold but in relation to how hot the other areas are it seems to be sufficient. It has been very reliable. We have had three in the 22 years I have used them and two are still in use. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gregoire, Rhonda (MAFRI) Sent: Tuesday, January 21, 2014 12:09 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Tissue-Tek TEC5 We are looking to purchase a new embedding centre. If you are using the Tissue-Tek embedding centre do you find that the cold spot is cold enough? Any other pros/cons? Thanks Rhonda Gregoire, MLT Supervisor, Clinical Pathology Veterinary Diagnostic Services Manitoba Agriculture, Food and Rural Development 545 University Crescent Winnipeg, MB R3T 5S6 phone 204-945-7641 fax 204-945-7646 email rhonda.grego...@gov.mb.camailto:rhonda.grego...@gov.mb.ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EBV EBNA3A antibody (ab16126, Abcam)
Hello to everyone!!! Has anyone used this antibody ? Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam) Could I please get a copy of your protocols? Thank you so much Aldana Vistarop ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] [Fwd: EBV EBNA3A antibody (ab16126, Abcam)]
Mensaje original Asunto: EBV EBNA3A antibody (ab16126, Abcam) De: avista...@ffyb.uba.ar Fecha: Mar, 21 de Enero de 2014, 5:45 pm Para: Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu -- Hello to everyone!!! Has anyone used this antibody ? Sheep polyclonal to EBV EBNA3A antibody (ab16126, Abcam) This antibody is used on FFPE tissue. Could I please get a copy of your protocols? Thank you so much Aldana Vistarop ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cap on number of antibodies that can be ordered per patient/case.
Hi Everyone I was approached by an administrator today who wanted to know if a limit (cap) existed on the number of antibodies which can be ordered on a patient. They currently run a minimum of two antibodies on all Esophagus cases as part of a panel. Now they would like to increase this by an additional 3. These cases are referenced based and are on cell blocks although a few of them would be small biopsies. I was to understand that doing a one size fits all panel was sort of iffy unless there is an established protocol that proves the necessity for reimbursement. I had heard about a cap on the number but can't find the documentation. Any and all input is greatly appreciated. Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histogel
The document at http://lists.utsouthwestern.edu/mailman/listinfo/histonet(http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf;http://www.thermo.com/eThermo/CMA/PDFs/Various/File_9759.pdf gives a thorough description of Histogel, and even says what it is - hydroxyethyl agarose. In the detailed instructions for various uses, the only confusing thing is the requirement for non-porous filter paper! John Kiernan Anatomy, UWO London, Canada = = = On 20/01/14, Elizabeth Chlipala l...@premierlab.com wrote: Esther I agree with Dusko, I fix before I put in histogel and again after the sample is placed in histogel, we have no formalin on our tissue processor, we start in 50% alcohol. I also process on a longer processing cycle, 1 hour per station and similar to Dusko's - denatured ethanol, xylene and paraplast and paraplast extra to embed. I've never had a problem (such as overprocessed tissue) with the histogel or the sample embedded in the histogel with the longer processing cycle. Most of the samples we process are cell blocks or tissue fragments such as micronized tissue constructs, which are like powder when we receive them. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] histonet-boun...@lists.utsouthwestern.edu] On Behalf Of dusko trajkovic Sent: Monday, January 20, 2014 12:47 PM To: Esther C Peters; jennifer.arcand-john...@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Esther, I mainly process cells, which have been spun down into a small pellet. Also mouse DRG's and other very small tissues. I would consider this delicate, so do not be afraid to use a longer processing program. Histogel/Agurose is what needs longer dehydrating steps. We do not use any substitute reagents, so in that aspect I cannot tell you how they will affect the processing. Our lab uses ethanol, xylene, and Paraplast paraffin. Try a test run and let me know if you were able to get successful results. Have a good Monday! Dusko From: Esther C Peters epete...@gmu.edu To: jennifer.arcand-john...@genzyme.com jennifer.arcand-john...@genzyme.com; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu; dusko trajkovic dunat...@sbcglobal.net Sent: Monday, January 20, 2014 11:15 AM Subject: RE: [Histonet] Histogel Thank you, Dusko! I have had the same problem with 1.5% agarose, and I tried starting the dehydration with 30% to 50% to 70% ethanol, and using different xylene substitutes. It appears that the variable whitening and shrinking happens after 100% reagent alcohol and in the xylene substitute (now using Richard-Allan Clear-Rite3). I've wondered if slow infiltration was the issue. I guess we'll try this longer processing, but I also work with delicate tissues that normally would be a short run (15 min in each reagent). Are your tissues thin/delicate/biopsy or cell preps or organ samples? No effect on them? Esther Esther C. Peters, Ph.D. Assistant Professor Environmental Science Policy George Mason University 4400 University Drive, MS 5F2 Fairfax, VA 22030- From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of dusko trajkovic dunat...@sbcglobal.net Sent: Monday, January 20, 2014 1:58 PM To: jennifer.arcand-john...@genzyme.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histogel Jennifer, You might have seen one of my posts from 2-3 years ago. I had the exact same problems you described. Could not get anyone to come up with a solution. I ran various programs on our VIP and finally came up with a solution. Fix your specimens as you normally would do. Drain of the fixative add your histogel (dissolved in hot water, which you have been doing), fill the mold with the histogel. Let solidify on ice or 4C in fridge. Place the solid histogel in a cassette and process on a 12 hour program. Since I have instituted this procedure, have not had one bad block to date. Longer processing is the answer, and nothing else. Good Luck. Dusko Trajkovic Pfizer Inc. La Jolla 858-638-6202 From: jennifer.arcand-john...@genzyme.com jennifer.arcand-john...@genzyme.com To: histonet@lists.utsouthwestern.edu Sent: Monday, January 20, 2014 7:56 AM Subject: [Histonet] Histogel Dear Histonetters, I have been reading up on the archives for info on Histogel. Previous posts discuss how they had
