[Histonet] Replacing VIP?
Hello there, I currently working with VIP-antirabbit marker to trace pain in PNS and CNS ( Alexa 488 secondary) in rat. Since it is expressing many areas in spinal cord and brain, I am running to huge difficulties to detect its up regulation with ordinary immune fluorescence techniques . I couldn’t find any colourful pictures in literatures also, that can help me to compare my results with others! Does any one has a suggestion for replacing my trouble maker with another antibody which is easier to detect? Cheers, Leila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Dako's Omnis
We are in the process of having the Omnis installed at our facility. We did a fairly extensive (side by side, as much as was possible) comparison of the Ventana Ultra, the Leica Bond, and the Dako Omnis instruments before deciding on the Omnis. I will be happy to share our experiences about the Omnis with you as we are discovering them. Michelle Lamphere, HT(ASCP) Lead Tech, Histology Department of Anatomic Pathology 1935 Medical District Dr. Dallas, TX 75235 214.456.2318 Message: 3 Date: Wed, 29 Jan 2014 17:17:16 -0800 From: joe joe powderhoun...@hotmail.com Subject: [Histonet] Dako's Omnis To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: col130-w274931d0c3fb83a214c58eab...@phx.gbl Content-Type: text/plain; charset=iso-8859-1 Can anyone share their experiences with me about Dako's new IHC / FISH instrument? Thanks in advance! Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?
which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abt...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking tiny specimens
Eyelashes are pointed at the end. Other body hair is not. I would re-use my tiny paintbrush over and over. No need to pull out an eyelash every day! Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: John Kiernan jkier...@uwo.ca Sent: Friday, January 31, 2014 4:21 AM To: Barbara Tibbs; Cheryl Crowder; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] marking tiny specimens Dedication to duty! Ouch! Did you ever run out of eyelashes? Why not a short eyebrow or forearm hair? JK = = = On 30/01/14, Barbara Tibbs barbara.ti...@accuratediagnosticlabs.com wrote: Are you mixing Eosin in the last two alcohols of your processor? That should work. If you are dotting the specimens with Eosin before placing in a cassette, the dye will wash out early in the processing. Also, I don't know if this helps you but when I had to ink mouse arteries I would glue one of my eyelashes to the end of a wood applicator stick and use the eyelash as a tiny paint brush dipped in India ink. I would do this under a dissecting microscope. Maybe you could do that with the eggs? Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu on behalf of Cheryl Crowder ccrow...@vetmed.lsu.edu Sent: Thursday, January 30, 2014 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking tiny specimens I am processing some extremely small specimens - pin tip size. These are eggs with a protein covering. I have tried using eosin to color the tissues before processing but the color came out before paraffin. The coating on the eggs will not absorb the dye. Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed. Thanks in advance, Cheryl Cheryl Crowder, BA, HTL(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?
Hi Dorothy, I buy Methyl Methacrylate from Fisher Scientific. -Damien L. Sent from my iPhone On Jan 31, 2014, at 12:42 PM, Dorothy Hu abt...@gmail.com wrote: which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abt...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gross lab seniors
We have several gross lab senior grossing stations that are vented outside. Our engineering asked today whether the airflow should be checked yearly like other exhaust hoods. Problem is there is not a door like other hoods of course and how would you measure the airflow? Recommended airflow is 500cfm however clearly the airflow at the working surface is not anything close to that. I wondered how anybody else monitors the gross lab seniors or do they at all. CAP used to ask about documentation for checking hoods however I can't recall them ever checking on grossing stations. We change filters annually only since they are vented outside. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gross lab seniors
We use a company called C-Scan Technologies, Phoenix, AZ. The way they test all our gross dissection stations is by testing for directional or smoke containment and face velocity. We also check th they external pathway is clear and if the unit has a filtering system, the filters are changed regularly. The air flow measurement is Feet per minute (FPM) for face velocity and includes width, height, depth and total square ft for the working area. They exhaust flow in CFM. Face velocity minimum requirement is 100 fpm, exhaust flow requirement is 500 cfm. Face velocity fluctuates depending on the room and the air exchange rate for the area. I have always felt the face velocity is most important to gross dissection personnel. There needs to be adequate draw away from the employee, no matter the physical conditions of the room. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: vickroy@mhsil.com To: histonet@lists.utsouthwestern.edu Date: Fri, 31 Jan 2014 12:46:08 -0600 Subject: [Histonet] Gross lab seniors We have several gross lab senior grossing stations that are vented outside. Our engineering asked today whether the airflow should be checked yearly like other exhaust hoods. Problem is there is not a door like other hoods of course and how would you measure the airflow? Recommended airflow is 500cfm however clearly the airflow at the working surface is not anything close to that. I wondered how anybody else monitors the gross lab seniors or do they at all. CAP used to ask about documentation for checking hoods however I can't recall them ever checking on grossing stations. We change filters annually only since they are vented outside. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?
Dorothy, I personally order from Sigma and I have to tell you that in all my years of working with methyl methacrylate, they have been the most reliable source. I must admit that I did once use Fisher for a brief period of time due to pricing concerns from the purchasing department as there was something of an issue with the hazardous shipping from Sigma being almost as much as the MMA price, but not the case with Fisher. Honestly though I only used Fisher for about a year to a year and a half and switched back because of an issue with a particular lot that I encountered. During that particular issue my MMA blocks had polymerized with a sort of amber tint and with a pleasant orange fruity smell. It was alarming to me because I was completely used to having made crystal clear resin blocks and now I was starting to really love this orange smell during block preparation at the grinder that used to be a pungent MMA smell thats not really good for you under prolonged or excessive exposure. As time went by I then started to notice a change in polymerization rates and even sometimes an incomplete polymerization with a rubbery surface. While I did not recall any serious issue with staining, I did notice that cutting thin sections became a bit inconsistent and troublesome at times. It was then that I needed to discover if it was something that I was now doing differently than in the several years past of consistency or the chemicals that I was using. I ordered a new bottle off MMA from Sigma, changing only one variable, and ran a test. Turned out that I was back instantly to the clear blocks that I was accustomed to creating and that the issue had to be the Fisher bottle/lot of MMA. I want to point out that I am NOT saying that the Fisher MMA is an unreliable source of MMA. It was in my opinion clearly an issue of a bad lot/batch of product. However, given my OCD tendencies within the laboratory, I quickly reminded myself that if it's not broke, don't fix it! Basically, I shouldn't let pricing sacrifice the quality and consistency that I was accustomed to experiencing as I only switched suppliers due to a request for a cheaper alternative by the purchasing department. Oh and one more thing, I found out later that the orange smell was due to the presence of either solution or residue from the orange cleaning solution that the glass bottles are subjected to prior to filling with chemicals so like I said, could have been a one in a million experience but my mind told me I could not take any more chances! :) Best Regards, Jack From: dml...@gmail.com Date: Fri, 31 Jan 2014 12:56:55 -0500 To: abt...@gmail.com Subject: Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? CC: histonet@lists.utsouthwestern.edu Hi Dorothy, I buy Methyl Methacrylate from Fisher Scientific. -Damien L. Sent from my iPhone On Jan 31, 2014, at 12:42 PM, Dorothy Hu abt...@gmail.com wrote: which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abt...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ventana LEAN team
Don't expect much. It is more of a way to market their products. Robert A. Eytalis Laboratory Manager robert-eyta...@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mcken...@gastrodocs.net] Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ventana LEAN team
I had a different experience with the workflow design team. A lot of good ideas. Good presentation provided to our upper management team. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Friday, January 31, 2014 2:16 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana LEAN team Don't expect much. It is more of a way to market their products. Robert A. Eytalis Laboratory Manager robert-eyta...@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.riversidemc.net%252Fk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=ea1b568633a08afeb5425493a27a6db54a0d23bb8cb8243d5b8a243a2cf24fb4 | https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.facebook.com%252FRiversideMCk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=d9259289ca3bda8d72d66f8c847c386ff6120657a75ddeb820dc3127051e2d8f From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mcken...@gastrodocs.net] Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonetk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonetk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HISTOPALOOZA April 25 - April 27
It's that time of the year again! The Georgia Society for Histotechnology will be having its annual symposium at The Lodge and Spa at Callaway Gardens located in Pine Mountain, Georgia. This is a Marriott property so you can expect the accommodations to be excellent. Please go to the GSH website and click on the link for the Lodge. www.histosearch.com/gshhttp://www.histosearch.com/gsh In the coming weeks I'll be offering little tidbits of information regarding the list of speakers and topics. Of course, I plan to give you my spin on things, too. Shhh hehe Billie Zimmerman MT(ASCP)QIHC GSH Secretary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ventana LEAN team
Whenever fresh eyes look at a process there can be new idea. I would have to agree. It's free. Robert A. Eytalis Laboratory Manager robert-eyta...@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.riversidemc.net%2f | http://mail.riversidehealthcare.net/owa/redir.aspx?C=qIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.URL=http%3a%2f%2fwww.facebook.com%2fRiversideMC From: Willis, Donna G. [donna.wil...@baylorhealth.edu] Sent: Friday, January 31, 2014 2:24 PM To: Eytalis, Robert A; Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: RE: Ventana LEAN team I had a different experience with the workflow design team. A lot of good ideas. Good presentation provided to our upper management team. Donna Willis, HT/HTL (ASCP) Anatomic Pathology Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eytalis, Robert A Sent: Friday, January 31, 2014 2:16 PM To: Amber McKenzie; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Ventana LEAN team Don't expect much. It is more of a way to market their products. Robert A. Eytalis Laboratory Manager robert-eyta...@riversidehealthcare.net Phone: (815) 935-7256 ext. 5186 (815) 935-7535 Fax (815) 935-7068 Riverside Medical Center 350 N. Wall Street - Kankakee, IL 60901 https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.riversidemc.net%252Fk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=ea1b568633a08afeb5425493a27a6db54a0d23bb8cb8243d5b8a243a2cf24fb4 | https://urldefense.proofpoint.com/v1/url?u=http://mail.riversidehealthcare.net/owa/redir.aspx?C%3DqIzWpCGMNkq2SuiIt1v_2X7GmC1aOtAI7VlOiEQ974hhCQzwyDH6yJklmjoBIK92OWDFwivhIZs.%26URL%3Dhttp%253A%252F%252Fwww.facebook.com%252FRiversideMCk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=d9259289ca3bda8d72d66f8c847c386ff6120657a75ddeb820dc3127051e2d8f From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mcken...@gastrodocs.net] Sent: Thursday, January 30, 2014 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LEAN team Has anyone had experience with Ventana's Lean team? I am correctly designing a new lab and they are coming out next tues to look at my workflow. Just wondering what to expect and how much they may change my current layout of my future lab :) Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonetk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v1/url?u=http://lists.utsouthwestern.edu/mailman/listinfo/histonetk=eEbcMSXJ22m%2Bwpc%2B3zaDpA%3D%3D%0Ar=tcwXtHSgC9tIe4wFOXToKDTqAni3dMiqUwIdlw6Tfz0%3D%0Am=Y%2BBGGpnrJHgfSEpRSmZ6zE7NHNx6wN%2Br3UlWUcp4E%2Bo%3D%0As=0e52ddbc65dba9078e38143111a399ffecbd263d55ae80f465e8f5b4d87abfbc ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing
Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?
Oh,that's awful! Fortunately,I've never had a problem with product from either company;comparable results. -Damien Sent from my iPhone On Jan 31, 2014, at 2:15 PM, Jack Ratliff ratliffj...@hotmail.com wrote: Dorothy, I personally order from Sigma and I have to tell you that in all my years of working with methyl methacrylate, they have been the most reliable source. I must admit that I did once use Fisher for a brief period of time due to pricing concerns from the purchasing department as there was something of an issue with the hazardous shipping from Sigma being almost as much as the MMA price, but not the case with Fisher. Honestly though I only used Fisher for about a year to a year and a half and switched back because of an issue with a particular lot that I encountered. During that particular issue my MMA blocks had polymerized with a sort of amber tint and with a pleasant orange fruity smell. It was alarming to me because I was completely used to having made crystal clear resin blocks and now I was starting to really love this orange smell during block preparation at the grinder that used to be a pungent MMA smell thats not really good for you under prolonged or excessive exposure. As time went by I then started to notice a change in polymerization rates and even sometimes an incomplete polymerization with a rubbery surface. While I did not recall any serious issue with staining, I did notice that cutting thin sections became a bit inconsistent and troublesome at times. It was then that I needed to discover if it was something that I was now doing differently than in the several years past of consistency or the chemicals that I was using. I ordered a new bottle off MMA from Sigma, changing only one variable, and ran a test. Turned out that I was back instantly to the clear blocks that I was accustomed to creating and that the issue had to be the Fisher bottle/lot of MMA. I want to point out that I am NOT saying that the Fisher MMA is an unreliable source of MMA. It was in my opinion clearly an issue of a bad lot/batch of product. However, given my OCD tendencies within the laboratory, I quickly reminded myself that if it's not broke, don't fix it! Basically, I shouldn't let pricing sacrifice the quality and consistency that I was accustomed to experiencing as I only switched suppliers due to a request for a cheaper alternative by the purchasing department. Oh and one more thing, I found out later that the orange smell was due to the presence of either solution or residue from the orange cleaning solution that the glass bottles are subjected to prior to filling with chemicals so like I said, could have been a one in a million experience but my mind told me I could not take any more chances! :) Best Regards, Jack From: dml...@gmail.com Date: Fri, 31 Jan 2014 12:56:55 -0500 To: abt...@gmail.com Subject: Re: [Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone? CC: histonet@lists.utsouthwestern.edu Hi Dorothy, I buy Methyl Methacrylate from Fisher Scientific. -Damien L. Sent from my iPhone On Jan 31, 2014, at 12:42 PM, Dorothy Hu abt...@gmail.com wrote: which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abt...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
