[Histonet] new IHC billing and Sunquest CoPathPlus
Besides manually entering IHC charges, has anyone worked out a way to automatically bill for 88342 and 88343 on cases in Sunquest CoPathPlus? Thanks Ronnie Houston, MS HT(ASCP)QIHC Anatomic Pathology Manager ChildLab, a Division of Nationwide Children's Hospital www.childlab.com 700 Children's Drive Columbus, OH 43205 (P) 614-722-5450 (F) 614-722-2899 ronald.hous...@nationwidechildrens.orgmailto:ronald.hous...@nationwidechildrens.org www.NationwideChildrens.orghttp://www.nationwidechildrens.org/ One person with passion is better than forty people merely interested. ~ E.M. Forster ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
We still run negative controls. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] cryosectioning, bubbles between slide and section
Hello, This may be a little late, but we use a mix of half 30% sucrose and half OCT to section. It works really well with 14 to 25 micron sections and no cooling or heating of the slide. Our tissue is very small though--embryonic spinal cord or brain from chick or mouse. I just change the way I pick it up to get rid of bubbles. Have you tried rolling the slide upwards when you pick it up (if that makes sense) as opposed to rolling it from left to right (or the opposite)? Also as our cryostat gets older, it tends to get more static and we cannot get rid of it. This causes the tissue to adhere to the slide faster, which definitely causes bubbles. That may the case, but I hope not!! Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Fri, Apr 25, 2014 at 2:58 AM, Christina Kreutzer christina.kreutze...@gmail.com wrote: Hello members, I would need some help regarding cryosectioning of spinal cord. I am currently establishing cryosectioning on the cryostat (Leica CM1950) for this tissue and am having some problems. I am cutting 4% PFA fixed and succrose infiltrated spinal cords, embedded in Tissue Tek at 10µm and a temperature of approximately -16°C . I get more or less smoth slices but once I let them adhere to the slide -directly from the knife, after straightening it carefully with a brush - I get massive air bubbles between the slide and the slice. I have experience cutting on cryostats and with different tissues and never have had this problem before. I tried to change the temperature of the chamber and/or chunk and tried to warm the slide before adhering the slice and I tried to cool the slide. But it didn't help. Do you think changing from 30% succrose to a mixture of Tissue Tek/Succrose or even Tissue Tek/PBS would help? Does anybody have an advice? Regards Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
I just wanted to clarify there are two types of negative controls, one is a negative reagent control and one is a tissue that is known negative. A lot of time, we tend to focus on the negative reagent control and forget about the negative tissue control. I know that a lot of smart folks out there on histonet will be able to give you some direction as far as how to write a policy to cover both but just wanted to clarify that the two types of negative controls are different and the negative reagent control may be abandoned if you are running a polymer detection kit but the known negative tissue controls are still required. Thanks Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.436.1369 dsi...@statlab.com | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
This is what I put in my procedure shortly after Richard sent his email. There wasn't a problem at inspection. Following his announcement at NSH/ISH Forum in Windsor, CT, Dr. Richard Carten sent an email to Histonet on July 17, 2102, regarding CAP guidelines for negative controls. The checklist will be changed to reflect that negative controls will no longer be required for polymer based procedures. The new wording contains the following statement: “Immunohistochemical tests using polymer-based systems (biotin-free) are sufficiently free of background reactivity to obviate the need for a negative reagent control and such controls may be omitted at the discretion of the laboratory director”. Dr. Stargel has approved the discontinuation of negative controls as of July 20, 2012. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 5:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cryosectioning - still bubbles between slide and section, spinal cord
Dear members, first I wanna thank all the people who responded to my first email (Andrea, Mesru and Emily).Your tips were very helpful. Unfortunately I am still fighting agains bubbles/ wrinkles/bubble-shaped wrinkles between my slices and the slide. During the last couple of days I tried: i) to adjust the temperature of the chamber and the sample, seems like -16°C brings the best slices ii) to cool the slide by putting it into the chamber and warming it with my thumb just the second I held it towards the slice to adher iii) to warm the slides by putting them on a 37°C warm heating-plate after attachement iv) to moisten the slides with a little bit of bidest before letting the slices attach and put these slides on a 37°C heating plate afterwards...that was kind of desperate try to straighten the slices slowely v) 3 different intermedia before embedding the sample in pure Tissue Tek, a) 30% Succrose, b) half 30% succrose and half Tissue Tek, c) half Tissue Tek half PBS but...nothing really helped. I still have wrinkles aka bubbles. Tomorrow I am going the cut perfusion fixed rat spinal cord, hoping that this makes a difference. Anyway, I think I have some options left 1) trying to cut on a different mikrotome and 2) Leica promised to send me some slide/embedding solution/blade samples that worked for other groups. Can anybody think of something I havent tried yet? Besides of raising slide thickness and cutting for freefloating procedure? I would be so thankful for any ideas/suggestions/tips! Regards Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Your experience with barcoding and materials tracking in grossing and histology
Hi all, I am giving a talk at NSH about implementing barcoding and materials tracking in histology. I have that experience with Cerner Copath ABT, but not other systems. I'd like to hear from others, especially with other systems, about their experiences in implementing barcoding in their lab with their own LIS, third party vendors (ie, Vantanage, Cerebro, Lab Lion, Dako, others). Pros and cons, lessons learned, etc that would help others starting down that path. The point of the talk is the generalities of implementation, not a marketing talk on any particular system, though I would like to know why you chose a given system, whether you were able to use your own LIS for barcoding or had to use a third party vendor. Thanks in advance for any and all information. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Negative Controls
On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Seventh Wave Pathology or Other Contract Pathology Labs!
Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs!
Here is contact person: Kim Sagartz President Chief Operating Officer Senior Director, Client Services Seventh Wave Laboratories LLC 743 Spirit 40 Park Drive, Suite 209 Chesterfield MO 63005 Telephone: 636-519-4885 Facsimile: 636-519-4886 www.7thwavelabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Oskins Sent: Tuesday, April 29, 2014 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs!
Jennifer, You can send your samples to us. We'd be happy to evaluate them for you. My contact information is below. I guess I should ask what kind of samples you have first though...! Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Oskins Sent: Tuesday, April 29, 2014 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4577 / Virus Database: 3931/7411 - Release Date: 04/28/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs!
Jennifer, If your samples happen to be of a non-human nature, let me suggest the following lab: Amy S. Porter, HT(ASCP) QIHC Michigan State University Investigative HistoPathology Laboratory William S. Spielman, Ph.D. - Director Department of Physiology / Human Pathology Biomedical Physical Sciences Building 567 Wilson Road - Room 2133 East Lansing, MI 48824-3320 Phone: 517-884-5026 Fax: 517-432-1368 port...@msu.edu www.humanpathology.msu.edu Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) doug.por...@caplab.org www.caplab.org The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, copying, forwarding or capture of this communication is strictly prohibited. If you have received this communication in error, please notify me immediately by return e-mail and delete this and all copies. Thank-you. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Oskins Sent: Tuesday, April 29, 2014 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Seventh Wave Pathology or Other Contract Pathology Labs! Histonetters- I am trying to help a co-worker find contact information for Seventh Wave Pathology. Does anyone have information for this Lab? Are there any other Histology/Pathology Labs that are recommended for processing, staining and evaluating tissue samples? Thank you so much in advance! Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4577 / Virus Database: 3931/7411 - Release Date: 04/28/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job Opening in East Tennessee
Blount Memorial Hospital in Maryville, TN will have a histology technician position opening in May. One of our histotechnician's is retiring after a long career for some well deserved time to do as she wants. We are located in east Tennessee just minutes from the beautiful Smoky Mountains National Park and experience 4 wonderful seasons! Applicants must meet the educational and training requirements necessary for certification by the American Society of Clinical Pathology as a Histology Technician, HTL, or have experience equal to certification. Salary commiserate with experience. Please respond via email with resume attached to: rg...@bmnet.com . Thank you Renee Grow, BA., HT (ASCP) rg...@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Tennessee Laws. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete this message from your computer. Direct questions to the Blount Memorial Hospital Privacy Officer at 865-977-4675. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP question ANP.22978 - Her2 assay validation
Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%).We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP question ANP.22978 - Her2 assay validation
From what I have read and understand you should be able to do a write up the retroactive review based on the PT results. What we did here was bought a microarray slide that came with the HER2/ER/PR results, ran them on our machine, and compared our results to the ones that came with the slide. Vanessa Perez Garcia Pathology Reference Lab 210-892-3746 210-892-3732 vpe...@pathreflab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Tuesday, April 29, 2014 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP question ANP.22978 - Her2 assay validation Hello all, I have been reading through the most recent revisions and want to see how others are handling this question. The explanation states that it is for new and existing assays and that if your validation does not meet current standards that you must supplement and bring it into compliance. Furthermore if you do not have any documentation from the initial validation the assay must be fully revalidated and documented. Our lab has been performing the Herceptest from Dako (FDA approved) since before 2008 and participating in the HER2 proficiency testing since it was first offered. We have our statistical results comparing our IHC patient results to FISH Her2 results since 2008 and we have always done well on our CAP proficiency testing (95%-100%).We do inter-pathologist result comparisons, using know CAP slides and have 95% to 100% agreements. What I do not have however is the original results of the slides that were stained to set up the original assay. Under these circumstances will we need to completely revalidate the assay, using the mandated 20+/20- cases, or can we simply do a retroactive formal review and write up of our past performances on our proficiency testing challenges? Thanks in advance for your help with this! Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Your experience with barcoding and materials tracking in grossing and histology
So we have the Vantage system with CoPath ABT. With CoPath you cannot use Vantage unless you get ABT. I actually like Vantage alot. There are some issues that make it a little difficult to use and I would love to talk to you directly since trying to put it all in an e-mail is crazy. If you want you can call my cell 856-905-1549. Sue Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about high complexity testing for histotechnologists
Does anyone know if there is a regulatory agency that requires high complexity testing certification for histotechnologists. Catherine Susan Delia, BS. HT. ASCP Chief Technologist, Anatomic Pathology University Hospital 150 Bergen Street E-151 Newark, New Jersey 07103 Phone: 973-972-5717 Cell: 908-391-1060 Fax: 973-972-5724 deli...@uhnj.orgmailto:deli...@uhnj.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
