RE: [Histonet] Slide oven temperature
60-68 degrees C ranges are preferred, above that temperature one can begin to cause many problems with; staining, nuclear bubbling, antigen retrieval to name a few problems that have been recorded. Peggy Wink does a great 30 min CEU presentation on some of these problems through the NSH Webinar Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian Sent: Monday, May 05, 2014 11:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide oven temperature Can someone tell me the range you set your slide drying oven at and what you base it on. thanks, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Wrinkles on Glutaraldehyde Fixed Tissue
Why do you use glutaraldehyde for eyes? Davidson's is a much better fixative for eyes. With Davidson's you have two fast penetrating ingredients and one slow acting fixative. Glut is only a fast fixative. Besides, the best institutions who deal with eyes only use Davidson's. Just my thoughts. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ricardo Leyva Sent: Tuesday, May 06, 2014 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue Dear Histonetter, I am having an issue getting rid of wrinkles on sections from 4% glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's tissue of interest is the retina, and I use glut to avoid retinal detachment (this is mainly on tissue that won't be immunostained). I normally section at 5µm, and fix the tissue 1 or 2 days. I set the water bath at a temperature that is 5-10 C below the wax melting point. I have tried adding ethyl alcohol to the water and the wrinkles remain-or takes very long for most of them to be gone. I have tried placing the sections in a 1:15 mix of ethanol and water-and have also tried 30% ethanol in water, both at RT, before placing the sections on the water bath; when I do this, the sections separate abruptly (once in the water bath) from the slide I use for transferring the tissue, causing tissue layer separation. When I work with 10% formalin, the section wrinkles disappear nicely in the water bath that contains water and a bit ethanol. Nonetheless, when it comes to 4% glut it just does not work. Any input in troubleshooting this is greatly appreciated. Best, Ricardo Leyva (858) 822-1629 rle...@ucsd.edu UCSD Shiley Eye Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Edit protocol for Shandon Gemini Varistainer
Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
My name is Cynthia James; I work in a histopathology lab in Melrose Park Illinois. We are told to change the solution(2-Methyl Butane) which we use in the Histobath to freeze tissues for frozen sections to something less flammable. I remember a solution was discussed that can be used instead of the which is less flammable than the IsoPentane that we are currently using. Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about this solution.I am available from 5:00 am to 1:30 pm monday-friday central time, if you know the company that is selling this solution please let me know. Thank you in advance Cynthia James P.S I can also be reached at this email address naje1...@yahoo.com or cynthia_haynes-ja...@luhs.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Award Spotlight - Ann Preece Scholarship
Awards Spotlight - Ann Preece Scholarship PolySciences, Inc. sponsors this $1000 award on a reimbursement basis. Polysciences has been sponsoring this award since 2004, Ann Preece passed in 2003. She was born November 12, 1923 in Boston, Massachusetts. She worked at the pathology lab at Scripps Memorial Hospital and authored A Manual for Histological Technician which was published in three editions by Little, Brown Co. I'm old and lucky enough to have a copy of her book in my lab and I'm sure many others out there may also have a copy. Award Criteria: This award will be given annually to a qualified applicant who has excelled in working with calcified/decalcified bone, other materials such as stents in vascular research or bioengineering development. The specimens can be human or animal in origin. This award will support educational or professional certification programs that are intended to: 1. Enhance techniques and methods presently utilized in the nominee's institution, and/or 2. Support the study of new techniques under consideration for adoption in the nominee's laboratory, and/or 3. Support continuing education or certification. The past recipients of this award are Sarah Mack, Carol Bain, Damien Laudier, Robert Skinner, Vicki Kalscheur, Linda Jenkins, Jack Radcliff, Helen Wimer, Gayle Callis and Nancy Troiano. I know anyone who reads this list will know that these individuals are recognized as the experts in the field with respects to bioengineered constructs, stents, undecalcifed and decalcified bone. If you know of anyone who excels and is passionate in this specialized area of histology then nominate them for this award. http://nsh.org/scholarships-awards/education/individual%20scholarships Happy Hump Day! Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.commailto:l...@premierlab.com www.premierlab.comhttp://www.premierlab.com/ March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Edit protocol for Shandon Gemini Varistainer
Lol, I had the same problem. Yes the manual does not thoroughly explain this. U have to delete the protocol from the active stains list, edit the protocol and make sure reagents are linked to the correct buckets, then add the protocol back to the active stain list. I'm not sitting in front of my stainer right now to walk you through screen by screen but that is the whole idea behind it. Delete it, edit it, then add it back. (When you delete it, it's not deleted from the stainer its only deleted from being an active stain protocol.) I hope this helps a little. If you need further help, let me know I'll go to my machine and get the step by step info for you. You can also call their customer service # and they should be able to walk u thru it. Thanks, Sheryl Stephenson Histotechnologist NJ Department of Agriculture Animal Health Diagnostic Labs -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Job Opportunity: Pathology Associates Of Greenville Texas is seeking a full-time Histotehnologist/Histotechnichian, to join out practice. We are a free standing anatomic pathology laboratory with hospital affiliations, staffed by five pathologists and two full-time Histotechnologists/Histotechnicians. Our laboratory is fully automated. We preform routine surgical pathology, few non-gyn cytology, special stains (Histochemistry) and Immunohistchemistry (IHC). Requirements for the successful applicant: Strong work ethics- Excellent Interpersonal Skills and willing to assist in Frozen sections and Maintain Policies and Procedures of The Histology Laboratory. Candidates are preferably ASCP Certified/Eligible. Interested applicants may fax their resume at 903-454-1716 E-mail address: eb...@yahoo.com Sent from Windows Mail___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Ovens
Does anyone use the Thermo High-Capacity Section Dryer Item number B3120202? I've never seen it in person and I was debating on getting it vs the Cardinal one: M7289-20 SD-II-120 Slide dryer II. Any advice? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Edit protocol for Shandon Gemini Varistainer
Call Thermo and talk to Sherry Anderson at x562. She is awesome! She just walked me through some programming issues this morning. Thermo (800) 522-7270 x562 Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Wednesday, May 07, 2014 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Edit protocol for Shandon Gemini Varistainer Can anyone tell me how to edit a program on the Gemini Varistainer? I need to add heat steps to our deparaffinization protocol, but the manual is not very helpful. Thanks, Deloris Carter, HT(ASCP) Shawnee Mission Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Hercept Kits
Hello everyone, Has anyone had a problem with recent lots of Dako Hercept Kits? Thank you, James E. Nutter Jr. BS, HT QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | james.e.nut...@questdiagnostics.com mailto:james.e.nut...@questdiagnostics.com | www.NicholsInstitute.com http://www.nicholsinstitute.com/ __ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unsubscribe from emails
Can you unsubscribe me from all the emails or tell me where to go to do so, please. Sent from Yahoo Mail on Android ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cryo Macrotome
Hi List, We are looking at doing whole mouse sectioning for imaging on a beta imaging system. Does anyone know of any other cryo macrotome systems other than the Leica CM3600 (http://www.leicabiosystems.com/specimen-preparation/sectioning/cryosectioning/details/product/leica-cm3600-xp/) ? It is way too large for what we need (only mice/rats) and way too expensive. Alternatively is there a standard macrotome out there for doing this sort of sectioning? What do people use for cutting full slices of human brain? Cheers Cam Cameron J. Nowell Research Facilities Manager Monash Institute of Pharmaceutical Sciences Monash University 399 Royal Parade (Mail address: 381 Royal Parade) Parkville, VIC, 3052 Australia Email: cameron.now...@monash.edumailto:cameron.now...@monash.edu Mobile: +61 422882700 Office: +61 9903 9587 LinkedIn: Profilehttp://au.linkedin.com/pub/cameron-nowell/23/57/884/ Research Gate: Profilehttp://www.researchgate.net/profile/Cameron_Nowell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
