[Histonet] cryoprotection and anti.freeze
Dear Members, I was wondering what cryoprotection protocols you are using for perfusion fixed tissues (30% succrose, 1:1 Tissue Tek/PBS etc). Why are you using what you are using and did you ever have any problems. Second, I wanted your opinion concerning leaving tisse in cryoprotection solution for several months and antibody reactivity and/or if somebody ever had problems with antibody reactivity on CPS (Glycerin, Ethylene Glycol, PO4 buffer and water). Thanks in advance Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Service Supervisory Position open
We have an opening for a Histology Service Supervisor position. Please see the posting below. Supervisor, Histology Services The Histology Service Supervisor is responsible for day-to-day oversight of the Histology Service. This includes supervision of facility staff, work flow oversight and contributing to the completion of the overall workload in the Histology Service. This includes supervision of facility staff, providing training and guidance as needed. This position also includes carrying out routine and advanced histological procedures and contributing to completion of the overall workload in the department. Required Skills: * Excellent verbal and written communication skills. * Must be able to communicate effectively with others verbally and in writing to clearly and effectively exchange information specifically in the development of protocols and interpretation of data. * Current computer skills including use of spreadsheet, word processing, e-mail and internet tools. Must be able to utilize electronic databases and information management systems to record, monitor and analyze workflow. * Must be able to function effectively in a team environment and work with frequent changes in schedules and priorities. Required Experience: * Bachelor's degree in a biological science with HT (ASCP) or HTL (ASCP) certification and eight to ten (8-10) years of histology experience as a certified Histotechnologist. * Experience with histological techniques using animal models is desirable. * Certification in immunohistochemistry (QIHC) is required although equivalent experience (2 or more years) in immunohistochemical techniques may be considered. * Three to five (3-5) years of supervisory or lead experience. * Supervisory or lead experience in a clinical or pharmaceutical setting is desirable. Interested applicants should apply on line at www.jax.org/careershttp://www.jax.org/careers, referring to job posting #4377 with a cover letter and resume. Lesley S. Bechtold Senior Manager, Histopathology Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 (phone) 207-288-6325 (fax) The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Position open in Boston Suburb in Pharma/Biotech
Hi. I am a scientific/pharma recruiter. One of my pharmaceutical/biotech customers is seeking to hire a contract IHC specialist. This is great opportunity to move out of a hospital setting or a processing lab (LabCorp, Quest, etc.) and in to a research/industry environment. Your efforts will support research and development of cancer and infectious disease drug candidates. This position is full-time, M-F, 9 to 5 (approx) and located in Waltham, MA just off of 95. If you are interested please send me a copy of your resumé (in MS Word format) to jbla...@astrixinc.com. Please also include your desired compensation. If this position is not right for you but you know of colleagues that may be more appropriate please forward them this information. Thanks! Below is a list of responsibilities: * Performs microtomy of formalin fixed paraffin embedded (FFPE) tissues. * Provides tissue processing and embedding support to all projects and programs including Discovery, Biologics, Safety Assessment, Cancer and Infectious Diseases. * Reviews and interprets Immunohistochemical and HE stained tissue sections by light microscopy and fluorescence microscopy for Cancer and Safety Assessment projects. * Provides biomarker support by performing routine staining of tissue specimens utilizing automated and manual IHC procedures. * Implement quantitative image analysis for dual stained IHC slides. * Primary work interactions with the pathologist as well as the in vivo bioscientists * Prepares data presentations to project meetings * Work with histopathology management to lead the implementation of the quantitative analysis of image data for IHC including localization and expression patterns in rodent preclnical tissue. * Document preparation including IHC validation reports and SOPs Jason Blaine Managing Director Email: jbla...@astrixinc.com Creating Value and Trust in the Business of Science™ CONFIDENTIALITY NOTICE: The information contained in this message may be CONFIDENTIAL and is for the intended addressee only. Any unauthorized use, dissemination of the information, or copying of this message is prohibited. If you are not the intended addressee, please notify the sender immediately and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP at NU
Oh yay! CAP is visiting us today. Why do they send an MT to do histo? And a snarky one at that.. Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 126, Issue 9
I'm a medical technologist working in anatomic pathology and I take offense to your post, Bernice. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, May 08, 2014 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 126, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. (no subject) (cynthia haynes) 2. Award Spotlight - Ann Preece Scholarship (Elizabeth Chlipala) 3. RE: Edit protocol for Shandon Gemini Varistainer (Stephenson, Sheryl) 4. (no subject) (histogi...@gmail.com) 5. Slide Ovens (Amber McKenzie) 6. RE: Edit protocol for Shandon Gemini Varistainer (Laurie Colbert) 7. Dako Hercept Kits (Nutter, James E) 8. Unsubscribe from emails (awill.imdpath...@yahoo.com) 9. Cryo Macrotome (Cameron Nowell) 10. cryoprotection and anti.freeze (Christina Kreutzer) 11. Histology Service Supervisory Position open (Lesley Bechtold) 12. Fisher Embedding Meda (scott munday) 13. Position open in Boston Suburb in Pharma/Biotech (Jason Blaine) 14. CAP at NU (Bernice Frederick) -- Message: 1 Date: Wed, 7 May 2014 09:52:41 -0700 (PDT) From: cynthia haynes naje1...@yahoo.com Subject: [Histonet] (no subject) To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 1399481561.31655.yahoomail...@web160604.mail.bf1.yahoo.com Content-Type: text/plain; charset=iso-8859-1 My name is Cynthia James; I work in a histopathology lab?in Melrose Park Illinois. We are told to change the solution(2-Methyl Butane) which?we use in the Histobath to freeze tissues for frozen sections to something less flammable. I remember a solution was?discussed that can be used instead of the?which is less flammable than the ?IsoPentane that we are currently using. Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about this solution.I am available from 5:00 am to 1:30 pm monday-friday central time, if you know the company that is selling this solution please let me know. ? Thank you in advance Cynthia James ? P.S I can also be reached at this email address naje1...@yahoo.com or cynthia_haynes-ja...@luhs.org -- Message: 2 Date: Wed, 7 May 2014 11:34:43 -0600 From: Elizabeth Chlipala l...@premierlab.com Subject: [Histonet] Award Spotlight - Ann Preece Scholarship To: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Message-ID: 14E2C6176416974295479C64A11CB9AE019C79E05A9E@SBS2K8.premierlab.local Content-Type: text/plain; charset=us-ascii Awards Spotlight - Ann Preece Scholarship PolySciences, Inc. sponsors this $1000 award on a reimbursement basis. Polysciences has been sponsoring this award since 2004, Ann Preece passed in 2003. She was born November 12, 1923 in Boston, Massachusetts. She worked at the pathology lab at Scripps Memorial Hospital and authored A Manual for Histological Technician which was published in three editions by Little, Brown Co. I'm old and lucky enough to have a copy of her book in my lab and I'm sure many others out there may also have a copy. Award Criteria: This award will be given annually to a qualified applicant who has excelled in working with calcified/decalcified bone, other materials such as stents in vascular research or bioengineering development. The specimens can be human or animal in origin. This award will support educational or professional certification programs that are intended to: 1. Enhance techniques and methods presently utilized in the nominee's institution, and/or 2. Support the study of new techniques under consideration for adoption in the nominee's laboratory, and/or 3. Support continuing education or certification. The past recipients of this award are Sarah Mack, Carol Bain, Damien Laudier, Robert Skinner, Vicki Kalscheur, Linda Jenkins, Jack Radcliff, Helen Wimer, Gayle Callis and Nancy Troiano. I know anyone who reads this list will know that these individuals are recognized as the experts in the field with respects to bioengineered constructs, stents, undecalcifed and decalcified bone. If you know of anyone who excels and is passionate in this specialized area of histology then nominate them for this award.
Re: [Histonet] Dako Hercept Kits
I'm using Dako Hercept Kit with lot number 20003874 and exp.date of 9-2014 and it is fine. Our previous kit was lot number 20003728, exp. date 8-2014 and it was fine. Hope this helps everyone. Cindy Cynthia Bulmer HT(ASCP)QIHC IHC Supervisor, CTPL Waco, TX On Wednesday, May 7, 2014 4:33 PM, Nutter, James E james.e.nut...@questdiagnostics.com wrote: Hello everyone, Has anyone had a problem with recent lots of Dako Hercept Kits? Thank you, James E. Nutter Jr. BS, HT QIHC(ASCP) Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.| Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| | james.e.nut...@questdiagnostics.com mailto:james.e.nut...@questdiagnostics.com | www.NicholsInstitute.com http://www.nicholsinstitute.com/ __ The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ann Preece Award
Hello Histonetters If you are keeping up with my Award Spotlight posts you'll know that the last award I focused on was the Ann Preece Scholarship. I just got off the phone with Ken Urban and we had a great chat, apparently prior to Polysciences sponsoring this award through the NSH, Ann Preece herself used to sponsor this award. Ken remembers receiving this award in the mid to late 1960's. He submitted a picture of a Movats Pentachrome performed on an undecalcifed bone sample. The award at that time consisted of a certificate, a copy of Ann's book; A Manual for Histologic Technicians and a $100.00. I just searched and what do you know -- this book is still available on line, just check out this link - http://www.amazon.com/Manual-Histologic-Technicians-Ann-Preece/dp/0316717657 that's just amazing. Well the reason for this post is that I wanted to update everyone with what I found out and that I would like to get some more info on this award just to fill in the blanks, so if there is anyone out there that can remember anyone else who received this award, or the dates it was given, etc. it would be great if you could e-mail me. Any information or stories about Ann would be appreciated. It's nice to know where these awards came from. Thanks and have a great day. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.commailto:l...@premierlab.com www.premierlab.comhttp://www.premierlab.com/ March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue
Hi Bea, Thank you for your response. The reason to 4% Glut use is just a PI request (really a matter of preference), and it is only on tissue that will not be immunostained. The ocular tissue I work with is rabbit. One thing I noticed is that the tissue that is fixed for 24h takes longer to become wrinkle-free, whereas the 48h (or more) fixed tissue seems to spread out in the waterbath with better consistency. I am aware of the use of Davidson's‹in fact you are the one who so patiently gave me recommendations on it, and for that I am grateful. Nonetheless, something I'd encounter with Davidson's each time is that tissue would shrink a bit too much after processing (all of my reagents‹EtOH, Xylenes, and Wax are set for about 1h20min each); I tried decreasing the processing time but the issue remains. Perhaps the processing time needs to be increased on my end to get better results. Ricardo On 5/7/14 7:13 AM, Bea DeBrosse-Serra bdebrosse-se...@isisph.com wrote: Why do you use glutaraldehyde for eyes? Davidson's is a much better fixative for eyes. With Davidson's you have two fast penetrating ingredients and one slow acting fixative. Glut is only a fast fixative. Besides, the best institutions who deal with eyes only use Davidson's. Just my thoughts. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ricardo Leyva Sent: Tuesday, May 06, 2014 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue Dear Histonetter, I am having an issue getting rid of wrinkles on sections from 4% glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's tissue of interest is the retina, and I use glut to avoid retinal detachment (this is mainly on tissue that won't be immunostained). I normally section at 5µm, and fix the tissue 1 or 2 days. I set the water bath at a temperature that is 5-10 C below the wax melting point. I have tried adding ethyl alcohol to the water and the wrinkles remain-or takes very long for most of them to be gone. I have tried placing the sections in a 1:15 mix of ethanol and water-and have also tried 30% ethanol in water, both at RT, before placing the sections on the water bath; when I do this, the sections separate abruptly (once in the water bath) from the slide I use for transferring the tissue, causing tissue layer separation. When I work with 10% formalin, the section wrinkles disappear nicely in the water bath that contains water and a bit ethanol. Nonetheless, when it comes to 4% glut it just does not work. Any input in troubleshooting this is greatly appreciated. Best, Ricardo Leyva (858) 822-1629 rle...@ucsd.edu UCSD Shiley Eye Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ihc billing
Would anybody be able to shed some light on the 2014 updated IHC billing. I understand the 88342 and 88343 codes but am looking for some clarification on the G0461 and G0462 codes. Do these pertain to multiple antibodies on one slide as the 88343 does or is the G0461 for the 1st antibody and G0462 for all remaining antibodies performed for one specimen site? Thank you in advance Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Employee Evaluation
Hello Histonet, I am a relatively new supervisor and have been tasked with conducting my first employee evaluation. Does anyone have any documentation or checklists they would like to share with me to help me get started please? Thanks, Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: ihc billing
The G codes are for Medicare/Medicaid. G0461 - the first and G0462 - each additional per SPECIMEN. 88342 is per BLOCK. And you understand 88343... Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hutton, Allison Sent: Thursday, May 08, 2014 2:21 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ihc billing Would anybody be able to shed some light on the 2014 updated IHC billing. I understand the 88342 and 88343 codes but am looking for some clarification on the G0461 and G0462 codes. Do these pertain to multiple antibodies on one slide as the 88343 does or is the G0461 for the 1st antibody and G0462 for all remaining antibodies performed for one specimen site? Thank you in advance Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryo Macrotome
Dear Cam, I can help you on this as we have supplied the Bright 5040 OTF/AS, fitted with large section conversion kit Tungsten Carbide tipped knife for this type of application. It has also been used for undecalcifiedadult Rat head. We also manufacture the Bright 8250 cryostat for larger sections in whole human brain and full size rats. See our website www.brightinstruments.com Regards Alan Bright Sent from my iPhone On 8 May 2014, at 03:52, Cameron Nowell cameron.now...@monash.edu wrote: Hi List, We are looking at doing whole mouse sectioning for imaging on a beta imaging system. Does anyone know of any other cryo macrotome systems other than the Leica CM3600 (http://www.leicabiosystems.com/specimen-preparation/sectioning/cryosectioning/details/product/leica-cm3600-xp/) ? It is way too large for what we need (only mice/rats) and way too expensive. Alternatively is there a standard macrotome out there for doing this sort of sectioning? What do people use for cutting full slices of human brain? Cheers Cam Cameron J. Nowell Research Facilities Manager Monash Institute of Pharmaceutical Sciences Monash University 399 Royal Parade (Mail address: 381 Royal Parade) Parkville, VIC, 3052 Australia Email: cameron.now...@monash.edumailto:cameron.now...@monash.edu Mobile: +61 422882700 Office: +61 9903 9587 LinkedIn: Profilehttp://au.linkedin.com/pub/cameron-nowell/23/57/884/ Research Gate: Profilehttp://www.researchgate.net/profile/Cameron_Nowell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Senior Histotech Position
We have a position open for a Senior HT/HTL, preferably with grossing experience for our Las Cruces lab. Interested parties can contact me directly for details. Joanne Clark, BAAS, HT(ASCP) Director of Histology Pathology Consultants of New Mexico 575-317-6403 jcl...@pcnm.com Disclaimer: This electronic message may contain information that is proprietary, confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Wrinkles on Glutaraldehyde Fixed Tissue
Hi Bea The problem with Glutaraldehyde is not its fixation but it's penetration rate. It is very slow to penetrate which is why EM tissue specimens must be diced to such small pieces. If you want to use Glut you will need to cut the tissue very thin and use the extended fixation time. Regards Tony Tony Reilly B.App.Sc, M.Sc Chief Scientist Anatomical Pathology Pathology Queensland PAH _ Health Services Support Agency| Department of Health Building 15, Level 1, 199 Ipswich Road WOOLLOONGABBA Queensland 4102 Ph: 07 3176 2412 Mob: 0402139411 Fax: 07 3176 2930 Email: tony.reil...@health.qld.gov.au | www.health.qld.gov.au -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ricardo Leyva Sent: Friday, 9 May 2014 4:16 AM To: Tony Reilly; Bea DeBrosse-Serra; histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wrinkles on Glutaraldehyde Fixed Tissue Hi Bea, Thank you for your response. The reason to 4% Glut use is just a PI request (really a matter of preference), and it is only on tissue that will not be immunostained. The ocular tissue I work with is rabbit. One thing I noticed is that the tissue that is fixed for 24h takes longer to become wrinkle-free, whereas the 48h (or more) fixed tissue seems to spread out in the waterbath with better consistency. I am aware of the use of Davidson'sin fact you are the one who so patiently gave me recommendations on it, and for that I am grateful. Nonetheless, something I'd encounter with Davidson's each time is that tissue would shrink a bit too much after processing (all of my reagentsEtOH, Xylenes, and Wax are set for about 1h20min each); I tried decreasing the processing time but the issue remains. Perhaps the processing time needs to be increased on my end to get better results. Ricardo On 5/7/14 7:13 AM, Bea DeBrosse-Serra bdebrosse-se...@isisph.com wrote: Why do you use glutaraldehyde for eyes? Davidson's is a much better fixative for eyes. With Davidson's you have two fast penetrating ingredients and one slow acting fixative. Glut is only a fast fixative. Besides, the best institutions who deal with eyes only use Davidson's. Just my thoughts. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ricardo Leyva Sent: Tuesday, May 06, 2014 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wrinkles on Glutaraldehyde Fixed Tissue Dear Histonetter, I am having an issue getting rid of wrinkles on sections from 4% glutaraldehyde fixed paraffin embedded ocular tissue. The laboratory's tissue of interest is the retina, and I use glut to avoid retinal detachment (this is mainly on tissue that won't be immunostained). I normally section at 5µm, and fix the tissue 1 or 2 days. I set the water bath at a temperature that is 5-10 C below the wax melting point. I have tried adding ethyl alcohol to the water and the wrinkles remain-or takes very long for most of them to be gone. I have tried placing the sections in a 1:15 mix of ethanol and water-and have also tried 30% ethanol in water, both at RT, before placing the sections on the water bath; when I do this, the sections separate abruptly (once in the water bath) from the slide I use for transferring the tissue, causing tissue layer separation. When I work with 10% formalin, the section wrinkles disappear nicely in the water bath that contains water and a bit ethanol. Nonetheless, when it comes to 4% glut it just does not work. Any input in troubleshooting this is greatly appreciated. Best, Ricardo Leyva (858) 822-1629 rle...@ucsd.edu UCSD Shiley Eye Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in
