[Histonet] open source NIS ELEMENTS
Hello, we have a Nikon Eclipse E600 with a DS 2Mv camera, it was a present from another lab and the dongle for the Software is missing. So before I am contacting Nikon if it is possible to get a free/cheap additional dongle, I wanted to ask you if somebody knows a capable open source equivalent to the NIS ELEMENTS softare. Regards Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin Leaching
Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin Leaching
We haven’t changed our mounting medium, but I’ll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it’s very possible I didn’t make that a point. I’ll try that. Any more tips are welcome still - thank you all! Adrienne On Jun 20, 2014, at 8:11 AM, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote: Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin Leaching
I'd advise checking your dehydration alcohols with a hydrometer. This bleeding happened to me once and it turned out the alcohols after the Eosin were not graded. My lab aide accidently used 100% only instead of 70%, 95% and then 100%. Anita Anita -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Friday, June 20, 2014 8:15 AM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin Leaching We haven't changed our mounting medium, but I'll have to check on the alcohol levels. When I stain (we hand stain only - very small lab), I always make sure the alcohol is higher than the eosin, but I have a lab assistant now, and it's very possible I didn't make that a point. I'll try that. Any more tips are welcome still - thank you all! Adrienne On Jun 20, 2014, at 8:11 AM, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote: Is your alcohol level as high as or higher than the level of eosin in the containers? If there is residual eosin left at the upper portion of the slide, that could be causing the problem. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ The information contained in, or attached to, this e-mail, may contain confidential information and is intended solely for the use of the individual or entity to whom it is addressed and may be subject to legal privilege. If you have received this e-mail in error you should notify the sender immediately by reply e-mail, delete the message from your system and notify your system manager. Please do not copy it for any purpose, or disclose its contents to any other person. The views or opinions presented in this e-mail are solely those of the author and do not necessarily represent those of the company. The recipient should check this e-mail and any attachments for the presence of viruses. The company accepts no liability for any damage caused, directly or indirectly, by any virus transmitted in this email. ___ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EM question
I have a question for those labs still performing Electron Microscopy for diagnosis. What do you do if you have a specimen that turns out to be unsatisfactory for evaluation after you have done all the prep work? Can you bill an 88348 (technical charge) without billing the professional fee (88348-26)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Eosin Leaching
We had the same problem. Increase your Alcohol/Reagents (following Eosin) times as well as using fresh ones. I use 80% for about 20 sec and (2)100% for a minute each and (3) Xylene Subs for 2 min each. I also use a fresh 80% and rotate the 100% each day. If using a Xylene Sub it could also be a possibility that your mounting medium is not compatible. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in advance for your help! Adrienne Adrienne Anderson, BS, HTL(ASCP) Histotechnologist Phylogeny, Inc. 1476 Manning Pkwy, Powell, Ohio 43605 Phone: (614) 846-6161 Fax: (877) 591-1815 This message, including any attachments, is confidential and may be privileged or may contain health information protected by state and federal law. Information and opinions expressed in this message and/or attachments are those of the author and are not necessarily those of the company. If you are not the intended recipient, please notify the sender and delete this message from your system. Any use of this information by individuals other than the intended recipient is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Bruce Palmatier is out of the office (returning 06/23/2014)
I am out of the office until 06/23/2014. I will be traveling on June 20th and will have limited access to email. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmat...@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: healthcareserv...@vwr.com Note: This is an automated response to your message Histonet Digest, Vol 127, Issue 25 sent on 6/20/2014 10:54:03 AM. This is the only notification you will receive while this person is away. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
