RE: [Histonet] please remove me
Hi Carolyn, You must go to this website http://lists.utsouthwestern.edu/mailman/listinfo/histonet and do this yourself. Best, Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolyn Thompson Sent: Saturday, July 12, 2014 5:14 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please remove me Carol Thompson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Storage of HER2 neu control slides
Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA
Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Derm Lab. This is a permanent full time position with benefits (medical/401k/vacation). ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com if interested. *full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *VERY proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone ksime...@leavittmgt.commailto:ksime...@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Storage of HER2 neu control slides
We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, July 14, 2014 8:44 AM To: Histonet (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Storage of HER2 neu control slides Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Storage of HER2 neu control slides
I do the complete drain dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC From: richard.car...@hhchealth.org To: lseb...@uwhealth.org; Histonet@lists.utsouthwestern.edu Date: Mon, 14 Jul 2014 14:22:43 + CC: Subject: [Histonet] RE: Storage of HER2 neu control slides We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, July 14, 2014 8:44 AM To: Histonet (Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Storage of HER2 neu control slides Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] please remove me
please remove me, thanks This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: please remove me
Please remove me, thanks Lisa LaVigne CT, MB(ASCP) Pathology Manager, St. Peter's Health Partners 315 S. Manning Blvd. Albany, NY 12208 518-525-5274 lisa.lavi...@sphp.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Huimin Wang Sent: Monday, July 14, 2014 10:39 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] please remove me please remove me, thanks This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Storage of HER2 neu control slides
Thanks Joelle. I'm surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30 @ 60 degrees vs 10 @ 60 degrees. I was testing some blocks that I had 3 cores of varying degrees of intensity. All showed much decreased reactivity at 30 vs 10. In fact, the 2 weaker cores were virtually negative. I can only assume you're using a different clone than we are and that there is different epitope stability between clones. Thanks for your response, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From: Joelle Weaver [mailto:joellewea...@hotmail.com] Sent: Monday, July 14, 2014 9:25 AM To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Storage of HER2 neu control slides I do the complete drain dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC From: richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org To: lseb...@uwhealth.orgmailto:lseb...@uwhealth.org; Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu Date: Mon, 14 Jul 2014 14:22:43 + CC: Subject: [Histonet] RE: Storage of HER2 neu control slides We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, July 14, 2014 8:44 AM To: Histonet (Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Storage of HER2 neu control slides Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Histology Opportunity - Chicago
Good morning! We have had a top company open a new Field Histology Specialist to cover the Chicago and Midwest Region. The company is a world leading histology manufacturer and this opportunity is perfect for a histology professional who is looking to break out of the laboratory and into the field. The position offers a competitive salary + bonus, company car, paid expenses, and full benefits. If you or someone you may know would be interested in learning more, please contact me directly at m...@personifysearch.com. Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 http://www.personifysearch.com/ www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Validation
Does anyone have a sample IHC Validation worksheet that they used in their lab for review. I know that the requirements have changed since our last inspection. I want to make sure that I am including all of the criteria, and would like to see how other labs are documenting this process. Leslie Graves Technical Specialist Cape Fear Valley Medical Center 1638 Owen Drive Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Marrow Trephine Cases
Over the past several years we have had some questions by our hematopathologists regarding the quality of the trephine blocks. The biggest concern has been that the sections seem to have a fragmented appearance which we have determined in the past to be areas where the lipid cell borders break. We have found that the artifact is diminished when we pick the sections up as quickly as we can which led us to believe that the cell borders break when they are left on the warm water in the waterbath. Some of the pathologists still believe the artifact is caused by the decalcification process. I have reviewed protocols before and obviously found that labs use different decalcifying agents from a harsh HCL solution to a weaker formic acid solution. We currently use Immunocal which I believe is a formic acid solution. I have also been introduced to some new instrumentation available such as the Milestone Bone Station which uses a EDTA solution with some added heat and agitation. I realize that EDTA yields more DNA and RNA which is obviously an advantage however the length of time for the decal process with EDTA is much longer. I'm not sure that using EDTA for decalcification will however reduce the artifact that the pathologists see (fragmentation and breaking up of the lipid cells in the sections. I would appreciate any information that others might be able to share from their experiences and if they have dealt with a similar problem. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 217-415-7505 Cell This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC for Adrenal hormones?
Is anyone doing IHC for adrenal corticosteroid and androgen hormones? We have an adrenal tumor in a 10 y.o. patient and the clinicians want to see what the tumor is producing. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Used Benchmark
I have a used Ventana Benchmark that is collecting dust in our storeroom. Would anyone be interested in this piece of equipment? I have pics and would be willing to answer any questions. When we put it in the store room it was in working order. Thanks, Travis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
