[Histonet] Consult for taking abs from RUO to IVD

[Patsy Ruegg]

Colleagues,

I am consulting with an antibody vendor who needs a consultant to help them 
take their abs from RUO to IVD.  If you know anyone who does this type of work 
you could recommend would you please have them contact me directly and I will 
pass their contact info on.

Best regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net
  
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[Histonet] Re: Weight Loss/Weight Gain decalcification endpoint test

[gayle callis]

Trevor and Jennifer, 

 

This method originally came from Mawhinney et al.  Control of rapid nitric
acid decalcification J Clin Pathol 1984 37:1409-1415 and was cited by Cathy
Sanderson (Mayton)in a publication using EDTA, found in Biotechnic and
Histochemistry.  Mawhinney acutally did a chemical test on final acid change
to see that calcium was not present, but we never had to do that.   If you
end up with a bit of residual calcium in block, I would surface decalcify at
microtomy.   I used it for years when we downsized and gave away our
FAXITRON.   Radiography is still the most accurate, and if you had either a
micro CT or digital FAXITRON available, it would be a better test. I
used Cathy's method and will be happy to send the original publication for
anyone's files/future referencing.   A chemical test for EDTA is a pain to
do if a FAXITRON is not available.   

 

I have put the full method below, with a bit more detail.   

 

WEIGHT LOSS/WEIGHT GAIN ENDPOINT TEST

 

This is a method of choice for EDTA decalcification although it was
originally used by Mawhinney et al for testing nitric acid decalcification.

Many samples can be decalcified together in one container, i.e. 25 mouse
femurs in 1 liter of 10% formic acid.   If all the samples are the same
size, i.e. mouse femurs, tibia, paws, choose several as representative
samples and test only those to save time.   Always suspend bones in the
decalcifier. 

Requires a balance that reads in milligrams to 3 places for greatest
accuracy.  Specimen must be blotted free of fluid for accurate weighing each
time you weigh the sample.  We suspend bones in nylon specimen bags for easy
removal to weigh.  Bags can be marked with pencil too. 

Technique:

 

1.  Rinse NBF off bone, blot with paper towel, WEIGH BONE, RECORD BEGINNING
WEIGHT.  Suspend bone in acid or EDTA decalcifier.  During acid
decalcification CO2 bubbles are given off, so stir during decalcification to
release bubbles or small samples will float.   EDTA does not create CO2
bubbles, only acids.  Large bones can be started at end of day in acid
decalcifier and sit overnight with testing the next morning.   

 

2. After 4 to 5 hours in acid or overnight in EDTA, remove bone, rinse with
water, BLOT, weigh.  RECORD WEIGHT.  If bone shows loss of weight, change
acid decalcifier to refresh acid.  Return samples to resume decalcification,
and repeat as many times as necessary.  EDTA should be changed but not as
often as acid.   Always use a large volume of decalcifier i.e. 20:1 or more.
Remove bone from specimen bag, and place in weighing boat to protect balance
from acids/EDTA. 

 

3.  When bone begins to GAIN WEIGHT, the bone is decalcified.  Once calcium
is removed, water is taken on and the weight increases.  This water does not
affect the bone.  

 

4.  Rinse bone with running tap water for an hour or longer to remove these
decalcifiers. Either store in 70% alcohol or process.Store endpoint
tested decalcified bones in 70% alcohol while waiting for other samples to
finish decalcifying and mass processing run.  

 

Reminders:  For EDTA, one can suspend bones and check every day for accuracy
but bones can be left in the EDTA over a weekend or several days without
damage as long as the bones were well fixed. Acid decalcified bones cannot
be left over a weekend, remove from acid, put in NBF to stop
decalcification. Bones should be endpoint tested before stopping
decalcification so you can resume decalcification on the next working day.
Rinse off NBF briefly before resuming decalcification.   Do not overexpose
bones to acids or you will damage antigens and nuclear staining.   

 

Enjoy the method, as it truly is fast and easy.

 

Gayle M. Callis

HTL/HT/MT(ASCP) 

 

 

 

 

 


_

Ha Wow...that's almost too easy. Thank you for this!

 

 

Trevor Jordan Wait

University of Texas Health Science Center, San Antonio

Class of 2017 MD Candidate

Abilene Christian University Class of 2013 Graduate

B.S.  Biochemistry



From: Jennifer MacDonald <
 JMacDonald <@t>
mtsac.edu>

Sent: Monday, August 11, 2014 2:33 PM

To: Wait, Trevor Jordan

Cc:   histonet
<@t> lists.utsouthwestern.edu;
 histonet-bounces
<@t> lists.utsouthwestern.edu

Subject: Re: [Histonet] Weight Loss/Weight Gain Decal

 

I believe this was originally from Patsy Ruegg

 

Decalcification End Point: Weight Loss, Weight Gain

 

 

1.Blot sample to remove excess fixative

2.Weigh bone in mg, record as beginning weight

3.Next day, rinse bone, blot and weigh bone daily, record weight.
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh
decalcifying solution.

4.When bone begins to GAIN weig

Re: [Histonet] RE:On the lighter side...

[Barry Rittman]

Well fixed in the histological sense I hope.
Barry


On Mon, Aug 11, 2014 at 2:52 PM, Mayer,Toysha N 
wrote:

> Tim,
>
> Just saw your post about the 'marketable skill'.  Funny those were my
> mother's exact words while I was in college.  She didn't care what I
> majored in, as long as I got a marketable skill along the way.
> The best advice I've ever gotten.
> Thanks Maria!!! (my mother)
>
> Sincerely,
>
> Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
> Instructor/Education Coordinator
> Program in Histotechnology
> School of Health Professions
> UT M.D. Anderson Cancer Center
> 713.563-3481
>
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
> Sent: 08 August 2014 19:26
> To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On the lighter side...
>
> Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369,
> 1992. Electron Microscopy Technologist, #604, 1982.
>
> Like most, I never heard of "histology" until I walked into a hospital lab
> on my first day as  an EM tech. I had seen slides made in college, but no
> one ever mentioned it could be an actual profession. I was more taken with
> the electron microscope, and there was (is) a 2-year program at the
> community college in the town I grew up in (Delta College, Stockton, CA).
> So AFTER getting a BA in Zoology, I went there to get a marketable skill.
> At that time EM was still used for tumor dx, so when I started it was about
> half tumor, half kidney. I was lucky enough to get involved in histology
> and set up the IHC lab at the small community hospital I worked at (as an
> EM tech) and so ended up phasing myself almost out of an EM job. The IHC
> took over all the tumor dx from EM. Later I left EM altogether and did IHC
> exclusively for 15 years. But, like most, I learned Histotechnology on the
> job but was lucky enough to work for a pathologist who believed in
> developing his techs - to the point of paying for meetings out of his own
> pocket. Only now do I know how fortunate I was to work for someone like
> that. Because of him we had developed a culture in the small histo lab (4
> men!!) of learning. We studied together one night a week for the HT exam
> and all passed (and the practical!). Again, that was a fortunate
> experience, not very often seen in labs.
>
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special
> Studies UC San Francisco Medical Center San Francisco, CA
>
>
>
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[Histonet] RE:On the lighter side...

[Mayer,Toysha N]

Tim,

Just saw your post about the 'marketable skill'.  Funny those were my mother's 
exact words while I was in college.  She didn't care what I majored in, as long 
as I got a marketable skill along the way.
The best advice I've ever gotten.  
Thanks Maria!!! (my mother)

Sincerely,

Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: 08 August 2014 19:26
To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 
1992. Electron Microscopy Technologist, #604, 1982. 

Like most, I never heard of "histology" until I walked into a hospital lab on 
my first day as  an EM tech. I had seen slides made in college, but no one ever 
mentioned it could be an actual profession. I was more taken with the electron 
microscope, and there was (is) a 2-year program at the community college in the 
town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in 
Zoology, I went there to get a marketable skill. At that time EM was still used 
for tumor dx, so when I started it was about half tumor, half kidney. I was 
lucky enough to get involved in histology and set up the IHC lab at the small 
community hospital I worked at (as an EM tech) and so ended up phasing myself 
almost out of an EM job. The IHC took over all the tumor dx from EM. Later I 
left EM altogether and did IHC exclusively for 15 years. But, like most, I 
learned Histotechnology on the job but was lucky enough to work for a 
pathologist who believed in developing his techs - to the point of paying for 
meetings out of his own pocket. Only now do I know how fortunate I was to work 
for someone like that. Because of him we had developed a culture in the small 
histo lab (4 men!!) of learning. We studied together one night a week for the 
HT exam and all passed (and the practical!). Again, that was a fortunate 
experience, not very often seen in labs. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA



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[Histonet] RE: Histonet Digest, Vol 129, Issue 17

[Mayer,Toysha N]

Egads, I feel like a baby!
20 years registered, 3 yrs unregistered.
By the way, I did see a former colleague with her ASCP certificate in her 
office, and the oldest sticker is from the year I was born.


Sincerely,

Dr.Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481


 


Message: 2
Date: Sun, 10 Aug 2014 21:50:58 +
From: Sebree Linda A 
Subject: RE: [Histonet] On the lighter side...
To: Jamal , 'Vincent Rivera'
, 'Douglas Porter' ,
"histonet@lists.utsouthwestern.edu"

Message-ID:
<77dd817201982748bc67d7960f2f76af0c7...@uwhc-mbx12.uwhis.hosp.wisc.edu>

Content-Type: text/plain; charset="iso-8859-1"

38 years and looking forward to retirement in a few years.

Linda A. Sebree

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RE: [Histonet] Weight Loss/Weight Gain Decal

[Wait, Trevor Jordan]

Ha Wow...that's almost too easy. Thank you for this!


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S.  Biochemistry

From: Jennifer MacDonald 
Sent: Monday, August 11, 2014 2:33 PM
To: Wait, Trevor Jordan
Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: Re: [Histonet] Weight Loss/Weight Gain Decal

I believe this was originally from Patsy Ruegg

Decalcification End Point: Weight Loss, Weight Gain


1.Blot sample to remove excess fixative
2.Weigh bone in mg, record as beginning weight
3.Next day, rinse bone, blot and weigh bone daily, record weight.  
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh 
decalcifying solution.
4.When bone begins to GAIN weight, remove from decalcifying solution, 
rinse and process.

YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY

Once calcium is totally removed (bone loses weight as this happens), water 
replaces the calcium and weight begins to go up.  This is the point at which 
calcium should be totally gone.  The original method used a chemical test at 
the end to insure no calcium was in the decalcifying solution.  If you do this, 
you cannot stir the solution during decalcification.  Be sure to suspend bone 
in the solution to insure all sides of bone are in contact with decalcification 
solution.



From:"Wait, Trevor Jordan" 
To:"histonet@lists.utsouthwestern.edu" 

Date:08/11/2014 12:29 PM
Subject:[Histonet] Weight Loss/Weight Gain Decal
Sent by:histonet-boun...@lists.utsouthwestern.edu




Hello all! I'm currently doing some decalcification and was curious if anyone 
had some particular advice about the weight loss/weight gain method. I 
understand that when the decalcification process is complete, the tissue block 
will begin to increase in weight. However, I'm confused when I should record 
the weight for the block once they have been taken out of the EDTA solution. 
You see, for the times I weighed the blocks before... the weights were a little 
skewed because there were differing amounts of solution on the blocks while 
they were sitting on the balance. I just want to standardize my protocol a 
little more so that I can be sure the block is actually gaining weight due to 
the calcium loss rather than just extra solution sitting on the outside of the 
block. Would letting the blocks sit out of solution for about 30 minutes before 
being weighed help with the matter? I know that the blocks take on water once 
they are completely decalcified so I'm not sure how much this will affect that.


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S.  Biochemistry
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Re: [Histonet] Weight Loss/Weight Gain Decal

[Jennifer MacDonald]

I believe this was originally from Patsy Ruegg

Decalcification End Point: Weight Loss, Weight Gain


1.  Blot sample to remove excess fixative
2.  Weigh bone in mg, record as beginning weight
3.  Next day, rinse bone, blot and weigh bone daily, record weight. 
Change decalcifying solution to refresh acid OR EDTA. Return bone to fresh 
decalcifying solution.
4.  When bone begins to GAIN weight, remove from decalcifying 
solution, rinse and process.

YOU MUST WEIGH IN MILLIGRAMS FOR ACCURACY

Once calcium is totally removed (bone loses weight as this happens), water 
replaces the calcium and weight begins to go up.  This is the point at 
which calcium should be totally gone.  The original method used a chemical 
test at the end to insure no calcium was in the decalcifying solution.  If 
you do this, you cannot stir the solution during decalcification.  Be sure 
to suspend bone in the solution to insure all sides of bone are in contact 
with decalcification solution. 



From:   "Wait, Trevor Jordan" 
To: "histonet@lists.utsouthwestern.edu" 

Date:   08/11/2014 12:29 PM
Subject:[Histonet] Weight Loss/Weight Gain Decal
Sent by:histonet-boun...@lists.utsouthwestern.edu



Hello all! I'm currently doing some decalcification and was curious if 
anyone had some particular advice about the weight loss/weight gain 
method. I understand that when the decalcification process is complete, 
the tissue block will begin to increase in weight. However, I'm confused 
when I should record the weight for the block once they have been taken 
out of the EDTA solution. You see, for the times I weighed the blocks 
before... the weights were a little skewed because there were differing 
amounts of solution on the blocks while they were sitting on the balance. 
I just want to standardize my protocol a little more so that I can be sure 
the block is actually gaining weight due to the calcium loss rather than 
just extra solution sitting on the outside of the block. Would letting the 
blocks sit out of solution for about 30 minutes before being weighed help 
with the matter? I know that the blocks take on water once they are 
completely decalcified so I'm not sure how much this will affect that.


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S.  Biochemistry
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[Histonet] Weight Loss/Weight Gain Decal

[Wait, Trevor Jordan]

Hello all! I'm currently doing some decalcification and was curious if anyone 
had some particular advice about the weight loss/weight gain method. I 
understand that when the decalcification process is complete, the tissue block 
will begin to increase in weight. However, I'm confused when I should record 
the weight for the block once they have been taken out of the EDTA solution. 
You see, for the times I weighed the blocks before... the weights were a little 
skewed because there were differing amounts of solution on the blocks while 
they were sitting on the balance. I just want to standardize my protocol a 
little more so that I can be sure the block is actually gaining weight due to 
the calcium loss rather than just extra solution sitting on the outside of the 
block. Would letting the blocks sit out of solution for about 30 minutes before 
being weighed help with the matter? I know that the blocks take on water once 
they are completely decalcified so I'm not sure how much this will affect that.


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
Abilene Christian University Class of 2013 Graduate
B.S.  Biochemistry
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RE: [Histonet] On the lighter side...

[Bernice Frederick]

Or we'll live forever.

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner
Sent: Monday, August 11, 2014 12:49 PM
To: Shirley A. Powell; Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

Saves on embalming costs

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Monday, August 11, 2014 1:04 PM
To: Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

I agree. :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...


Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, ("what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) 
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, "Edwards, Richard"  wrote:

>Sniffed my  first formalin and  saw  first post-mortem November 1965.
>
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RE: [Histonet] On the lighter side...

[Mark Turner]

Saves on embalming costs

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shirley A. 
Powell
Sent: Monday, August 11, 2014 1:04 PM
To: Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

I agree. :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...


Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, ("what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) 
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, "Edwards, Richard"  wrote:

>Sniffed my  first formalin and  saw  first post-mortem November 1965.
>
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RE: [Histonet] Are Paraffin Blocks Biohazard

[Michael LaFriniere]

Dawn,

The only study I know of is on CJD crutsfeldt-Jacobs Disease (know to survive 
formalin fixation and routine processing protocols, the CDC web site has 
additional information, In my laboratories I put all blocks in hazardous waste 
for incineration disposal.  It is not that costly just to be on the safe side. 



Michael R. LaFriniere, HT (ASCP) 
Executive Director
 

Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A * Silver Spring, MD 20904  
P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844
michael.lafrini...@ccplab.com
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawn Bugge
Sent: Friday, August 08, 2014 2:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Are Paraffin Blocks Biohazard

Hello Histo World!

Our pathologist for our private GI lab would like me to find out if anyone
has done a study to determine if the paraffin blocks, once they have been
processed, are considered biohazard.  I have searched high and low and can
find many people stating that the blocks are not bioharzard, with the
exception of neurological tissue, but they don't state how they know this.
He would like me to reference an actual study to prove that someone has
actually looked into this.

Any one know of something like this?  I know common sense would say that
once the tissues have been in formalin for hours, than on the processor for
hours that the tissue would be non biohazard and completely safe.

Thanks for your help :)

-- 
Dawn R Bugge HT(ASCP), Lab Manager
Seattle Histology
Dawns Usborne Books Website 
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[Histonet] Bcl-2

[Heather Knight]

Hi everyone-

Just wondering if anyone has a working protocol for Bcl-2 in FFPE mouse
tissue?  If so, can you share both the protocol and the antibody
information?

We have tried numerous antibodies over the years with very limited success.
 Thank you for your help!!

Best,
Heather Knight
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[Histonet] IHC Steam Strips:

[Jb]

Does anyone use IHC steam strips in their decloaking chamber?  The question is 
do you run a new steam strip each run and log each individual run w/it's own 
steam strip?  

Thank you,

Sent from my iPhone
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RE: [Histonet] On the lighter side...

[Shirley A. Powell]

I agree. :)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Monday, August 11, 2014 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...


Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, ("what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP) 
Histology Manager Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, "Edwards, Richard"  wrote:

>Sniffed my  first formalin and  saw  first post-mortem November 1965.
>
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[Histonet] Need microvascular (MVD) staining and quantification of tumro vasculature.

[Hans B Snyder]

Hello All,

I have a customer who needs microvascular (MVD) staining and
quantification of tumro vasculature, where staining is typically done
using CD31.  Is there anyone who can do this and willing to forward
their information to me?

Thank you

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com

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[Histonet] Ventana Symphony

[Fawn Bomar]

Can anyone using the Ventana Symphony share its pros and cons with me?  Would 
you recommend this stainer?



Thank you

Fawn
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Re: [Histonet] On the lighter side...

[Michael Ann Jones]

Oh,
I ment to say “Lots of tenure here and lots of brain cells here” meaning
this website and all histotechs here, not myself.
Obviously I don’t have many brain cells left If I made it look like I was
talking about myself. . .sheesh. .

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 10:18 AM, "Bernice Frederick" 
wrote:

>It was Friday,I was getting goofy doing paperwork,...
>
>Bernice Frederick HTL (ASCP)
>Senior Research Tech
>Pathology Core Facility
>ECOGPCO-RL
>Robert. H. Lurie Cancer Center
>Northwestern University
>710 N Fairbanks Court
>Olson 8-421
>Chicago,IL 60611
>312-503-3723
>b-freder...@northwestern.edu
>
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
>Ann Jones
>Sent: Monday, August 11, 2014 9:07 AM
>To: Edwards, Richard; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] On the lighter side...
>
>25 years, ("what¹s that in micron¹s?²) Bernice, you are too funny!!
>(lots of tenure here . . .lotsa brain cells) Michael Ann Jones, HT (ASCP)
>Histology Manager Metropath
>7444 W. Alaska Dr. #250
>Lakewood, CO 80226
>303.634.2511
>mjo...@metropath.com
>
>
>
>
>On 8/11/14, 5:17 AM, "Edwards, Richard"  wrote:
>
>>Sniffed my  first formalin and  saw  first post-mortem November 1965.
>>
>>
>> 
>>  Richard  Edwards
>>
>> 
>>   Leicester   U.K.
>>
>>-Original Message-
>>From: histonet-boun...@lists.utsouthwestern.edu
>>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
>>Timothy
>>Sent: 08 August 2014 19:26
>>To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
>>Subject: RE: [Histonet] On the lighter side...
>>
>>Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL
>>1369, 1992. Electron Microscopy Technologist, #604, 1982.
>>
>>Like most, I never heard of "histology" until I walked into a hospital
>>lab on my first day as  an EM tech. I had seen slides made in college,
>>but no one ever mentioned it could be an actual profession. I was more
>>taken with the electron microscope, and there was (is) a 2-year program
>>at the community college in the town I grew up in (Delta College,
>>Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a
>>marketable skill. At that time EM was still used for tumor dx, so when
>>I started it was about half tumor, half kidney. I was lucky enough to
>>get involved in histology and set up the IHC lab at the small community
>>hospital I worked at (as an EM tech) and so ended up phasing myself
>>almost out of an EM job. The IHC took over all the tumor dx from EM.
>>Later I left EM altogether and did IHC exclusively for 15 years. But,
>>like most, I learned Histotechnology on the job but was lucky enough to
>>work for a pathologist who believed in developing his techs - to the
>>point of paying for meetings out of his own pocket. Only now do I know
>>how fortunate I was to work for someone like that. Because of him we
>>had developed a culture in the small histo lab (4 men!!) of learning.
>>We studied together one night a week for the HT exam and all passed
>>(and the practical!). Again, that was a fortunate experience, not very
>>often seen in labs.
>>
>>Tim Morken
>>Supervisor, Histology, Electron Microscopy and Neuromuscular Special
>>Studies UC San Francisco Medical Center San Francisco, CA
>>
>>
>>-Original Message-
>>From: histonet-boun...@lists.utsouthwestern.edu
>>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas
>>Porter
>>Sent: Thursday, August 07, 2014 11:39 AM
>>To: histonet@lists.utsouthwestern.edu
>>Subject: [Histonet] On the lighter side...
>>
>>How long have you been a registered histotech?  36 years here.  You???
>>
>> 
>>
>>Douglas A. Porter, HT (ASCP)
>>Grossing Technician
>>IT Coordinator
>>
>>Cancer Registrar
>>
>>
>>CAP-Lab, PLC
>>2508 South Cedar Street
>>Lansing, MI 48910-3138
>>
>>517-372-5520 (phone)
>>517-372-5540 (fax)
>>
>>  doug.por...@caplab.org
>>
>>  www.caplab.org
>>
>> 
>>
>>___
>>Histonet mailing list
>>Histonet@lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>___
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>>
>>___
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>
>
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RE: [Histonet] On the lighter side...

[Ingles Claire]

Old histologists never die, they are just well fixed...
Claire

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones 
[mjo...@metropath.com]
Sent: Monday, August 11, 2014 9:07 AM
To: Edwards, Richard; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] On the lighter side...

25 years, ("what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, "Edwards, Richard"  wrote:

>Sniffed my  first formalin and  saw  first post-mortem November 1965.
>
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[Histonet] Anti MMP-9

[Johns, Laura [ETHUS]]

Hello,

Can anyone recommend an antibody for MMP-9 that works in FFPE porcine tissue?

Thanks,

Laura
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Re: [Histonet] On the lighter side...

[Michael Ann Jones]

25 years, ("what¹s that in micron¹s?²) Bernice, you are too funny!!
(lots of tenure here . . .lotsa brain cells)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/11/14, 5:17 AM, "Edwards, Richard"  wrote:

>Sniffed my  first formalin and  saw  first post-mortem November 1965.
>
>
>  
>  Richard  Edwards
>
>  
>   Leicester   U.K.
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken,
>Timothy
>Sent: 08 August 2014 19:26
>To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
>Subject: RE: [Histonet] On the lighter side...
>
>Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL
>1369, 1992. Electron Microscopy Technologist, #604, 1982.
>
>Like most, I never heard of "histology" until I walked into a hospital
>lab on my first day as  an EM tech. I had seen slides made in college,
>but no one ever mentioned it could be an actual profession. I was more
>taken with the electron microscope, and there was (is) a 2-year program
>at the community college in the town I grew up in (Delta College,
>Stockton, CA). So AFTER getting a BA in Zoology, I went there to get a
>marketable skill. At that time EM was still used for tumor dx, so when I
>started it was about half tumor, half kidney. I was lucky enough to get
>involved in histology and set up the IHC lab at the small community
>hospital I worked at (as an EM tech) and so ended up phasing myself
>almost out of an EM job. The IHC took over all the tumor dx from EM.
>Later I left EM altogether and did IHC exclusively for 15 years. But,
>like most, I learned Histotechnology on the job but was lucky enough to
>work for a pathologist who believed in developing his techs - to the
>point of paying for meetings out of his own pocket. Only now do I know
>how fortunate I was to work for someone like that. Because of him we had
>developed a culture in the small histo lab (4 men!!) of learning. We
>studied together one night a week for the HT exam and all passed (and the
>practical!). Again, that was a fortunate experience, not very often seen
>in labs. 
>
>Tim Morken
>Supervisor, Histology, Electron Microscopy and Neuromuscular Special
>Studies UC San Francisco Medical Center San Francisco, CA
>
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas
>Porter
>Sent: Thursday, August 07, 2014 11:39 AM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] On the lighter side...
>
>How long have you been a registered histotech?  36 years here.  You???
>
> 
>
>Douglas A. Porter, HT (ASCP)
>Grossing Technician
>IT Coordinator
>
>Cancer Registrar 
>
>
>CAP-Lab, PLC 
>2508 South Cedar Street
>Lansing, MI 48910-3138
>
>517-372-5520 (phone)
>517-372-5540 (fax)
>
>  doug.por...@caplab.org
>
>  www.caplab.org
>
> 
>
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>
>
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[Histonet] Laboratory Refrigerator w glass doors

[Susan Foreman]

We are looking at a new laboratory refrigerator >23ft for our IHC
department.  What models would you recommend and which to avoid?  

 

It's a Monday!

 

Susan Foreman, HT (ASCP)
KDL Pathology

315 Erin Drive

Knoxville, TN 37919

(865)584-1933

 

 

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RE: [Histonet] On the lighter side...

[Edwards, Richard]

Sniffed my  first formalin and  saw  first post-mortem November 1965.



Richard  Edwards


 Leicester   U.K.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: 08 August 2014 19:26
To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the lighter side...

Wow, I feel like a newbie! 28 years, registered. HT 13663, 1988, HTL 1369, 
1992. Electron Microscopy Technologist, #604, 1982. 

Like most, I never heard of "histology" until I walked into a hospital lab on 
my first day as  an EM tech. I had seen slides made in college, but no one ever 
mentioned it could be an actual profession. I was more taken with the electron 
microscope, and there was (is) a 2-year program at the community college in the 
town I grew up in (Delta College, Stockton, CA). So AFTER getting a BA in 
Zoology, I went there to get a marketable skill. At that time EM was still used 
for tumor dx, so when I started it was about half tumor, half kidney. I was 
lucky enough to get involved in histology and set up the IHC lab at the small 
community hospital I worked at (as an EM tech) and so ended up phasing myself 
almost out of an EM job. The IHC took over all the tumor dx from EM. Later I 
left EM altogether and did IHC exclusively for 15 years. But, like most, I 
learned Histotechnology on the job but was lucky enough to work for a 
pathologist who believed in developing his techs - to the point of paying for 
meetings out of his own pocket. Only now do I know how fortunate I was to work 
for someone like that. Because of him we had developed a culture in the small 
histo lab (4 men!!) of learning. We studied together one night a week for the 
HT exam and all passed (and the practical!). Again, that was a fortunate 
experience, not very often seen in labs. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
Sent: Thursday, August 07, 2014 11:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] On the lighter side...

How long have you been a registered histotech?  36 years here.  You???

 

Douglas A. Porter, HT (ASCP)
Grossing Technician
IT Coordinator

Cancer Registrar 


CAP-Lab, PLC 
2508 South Cedar Street
Lansing, MI 48910-3138 

517-372-5520 (phone)
517-372-5540 (fax) 

  doug.por...@caplab.org 

  www.caplab.org   

 

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[Histonet] Cytospin validation

[Jamal]

Hi

I need to do validation for my Shandon Cytospin 4, can anyone send me the
validation procedure and form.

 

 

Best Regards,

 

 

Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
 j.rowa...@alborglaboratories.com 

Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
 www.alborglaboratories.com

 

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RE: [Histonet] On the lighter side...

[Susan.Walzer]

For me 36years..will not be 37, I retire in 3 weeks! Off to visit my son 
and family in England!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar
Sent: Friday, August 08, 2014 11:01 AM
To: Elizabeth Chlipala
Cc: histonet@lists.utsouthwestern.edu; Cartun, Richard
Subject: Re: [Histonet] On the lighter side...

3 years in histology, 2 years registered!

Beth Brinegar HTL (ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403

Beth Brinegar HTL(ASCP)
Anatomic Pathology Supervisor
Mercy Medical Center
Cedar Rapids, IA 52403


On Fri, Aug 8, 2014 at 9:53 AM, Elizabeth Chlipala 
wrote:

> Too funny Richard - you would pass with flying colors
>
> For me its 31 years - HTL-930
>
> I have really been blessed, I love my job and I have really enjoyed my 
> career.  Every day there is something new to learn or to work on, for 
> example the lab is putting the final touches on a poster that will be 
> displayed at NSH this year, working on that has been exciting and a 
> lot of fun too.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO 
> Box 18592 Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> l...@premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> From: histonet-boun...@lists.utsouthwestern.edu [ 
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, 
> Richard [ richard.car...@hhchealth.org]
> Sent: Friday, August 08, 2014 8:32 AM
> To: Douglas Porter; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] On the lighter side...
>
> "36" years; however, not registered.   Hopefully, I can take the QIHC
> someday and pass.
>
> Richard
>
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs Assistant Director, Anatomic 
> Pathology Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 Office
> (860) 545-2204 Fax
> richard.car...@hhchealth.org
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter
> Sent: Thursday, August 07, 2014 2:39 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] On the lighter side...
>
> How long have you been a registered histotech?  36 years here.  You???
>
>
>
> Douglas A. Porter, HT (ASCP)
> Grossing Technician
> IT Coordinator
>
> Cancer Registrar
>
>
> CAP-Lab, PLC
> 2508 South Cedar Street
> Lansing, MI 48910-3138
>
> 517-372-5520 (phone)
> 517-372-5540 (fax)
>
>   doug.por...@caplab.org
>
>   www.caplab.org
>
>
>
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