[Histonet] A sticky subject....
Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: A sticky subject....
I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: A sticky subject....
I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu wrote: I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: A sticky subject....
Before I start cutting I spray StaticGard on the arms of my lab coat. I don't know what lotion you use but a non lanolin based moisturizer helped me (corn huskers lotion - it is glycerin based). This works for some but it comes down to trying different things to see what will work for you. Vikki On Dec 30, 2014 8:53 AM, Patrick Laurie foreig...@gmail.com wrote: I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu wrote: I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: A sticky subject....
Try Google for anti-static brush. There are several listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie Sent: Tuesday, December 30, 2014 7:54 AM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; Davis, Cassie Subject: Re: [Histonet] RE: A sticky subject I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu wrote: I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: QC daily HEs
Teri, our AM techs (ASCP) check the QC slide and sign off on it. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Monday, December 29, 2014 10:09 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] QC daily HEs What are the CLIA/CAP labs doing for daily HE QC? Does a pathologist need to review the HE control slide daily, or do you have an ASCP certified tech delegated to doing this? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sticky subject responses...Thank you
To all you wonderful folks who responded, a big thanks! I've been doing histo since 1990 and NEVER had static like today, it's not usually that big of a problem in this mid-Atlantic state. When it does occur the tricks I named earlier usually help but I am always up for learning something new. I will try the low tech option of moist something in my clean-up tray tomorrow, first. I usually can't stand anything in my tray after facing but if given a choice I'll suck it up. I will keep you posted. Thanks again! Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: A sticky subject....
A humidifier and a cool room temp does the job for us. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Victoria Baker [bakevicto...@gmail.com] Sent: Tuesday, December 30, 2014 8:58 AM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Davis, Cassie; Marcum, Pamela A Subject: Re: [Histonet] RE: A sticky subject Before I start cutting I spray StaticGard on the arms of my lab coat. I don't know what lotion you use but a non lanolin based moisturizer helped me (corn huskers lotion - it is glycerin based). This works for some but it comes down to trying different things to see what will work for you. Vikki On Dec 30, 2014 8:53 AM, Patrick Laurie foreig...@gmail.com wrote: I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu wrote: I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing
[Histonet] Re: Histonet Digest, Vol 133, Issue 30
I use Anti-Static Static-Away brush from Ted Pella Inc. Product no: 51922 5.7cm Handle length www.tedpella.com it works very well, I clean the metal surfaces of the paraffin microtome and do not wet the brush. Good Luck, Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: A sticky subject....
They sell water based anti static sprays for computer clean rooms and such. https://shop.eis-inc.com/sap(bD1lbiZjPTAwMQ==)/bc/bsp/sap/zeis2/index.htm?prod_area=371 They work great. Just be sure to spray the floor and the soles of your shoes, as well as the microtome and benchtop. Also, some microtomes have a grounding strap or a place to attach a grounding strap. Or you can wear a ground strap on your wrist, although this is a little restrictive. The best solution IMHO, ground your microtome and use professional anti-static spray (Not from the grocery store.) Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Dec 30, 2014 at 9:36 AM, Boyd, Debbie M dkb...@chs.net wrote: A humidifier and a cool room temp does the job for us. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] on behalf of Victoria Baker [ bakevicto...@gmail.com] Sent: Tuesday, December 30, 2014 8:58 AM To: Patrick Laurie Cc: histonet@lists.utsouthwestern.edu; Davis, Cassie; Marcum, Pamela A Subject: Re: [Histonet] RE: A sticky subject Before I start cutting I spray StaticGard on the arms of my lab coat. I don't know what lotion you use but a non lanolin based moisturizer helped me (corn huskers lotion - it is glycerin based). This works for some but it comes down to trying different things to see what will work for you. Vikki On Dec 30, 2014 8:53 AM, Patrick Laurie foreig...@gmail.com wrote: I used to have one of those anti-static brushes, it had a small radioactive piece of metal that discharged the static. It worked sometimes. Try putting moistened gauze, or the kimwipes used to clean your waterbath in your microtome wax waste tray. That seems to help me. Good luck. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Dec 30, 2014 at 8:45 AM, Marcum, Pamela A pamar...@uams.edu wrote: I am not sure where you live so this may or may not apply. I have lived in several placed and one I learned no matter where I am when they turn on the heat and you wear anything with polyester or that can become electrostatic we had issues with static. I am not sure how to fit it as I have made sure I touch something metal to discharge the static. It does not cure the problem only humidity will and being careful not to start to cut fully charged so to say. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Tuesday, December 30, 2014 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A sticky subject Good morning Histo folks, I am hoping somebody can help me. Today was a horrid microtomy day, the static was brutal. I tried putting lotion on my hands, the fabric softener sheet, and wearing gloves but to no avail. I heard rumors somebody used to manufactor an anti-static brush but have yet to find one. Does anybody have any other tricks that could be shared? Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___
RE: [Histonet] formaldehyde concentration
Thank you I should give more details -I am not actually doing histology but trying to find a method to preserve tiny plant shoot apical meristems (approximately 0.05mm cubed in volume) so I can then measure the amount of light organic elements (CONPS) in a section by using x-ray dispersive spectroscopy with scanning electron microscopy. I thought a variant of Karnovskys fixative with no buffer may do it-mix equal amounts of formaldehyde (say 16%% pure with no methanol and not 10% buffered formalin) and glutaraldehyde (25% EM grade)-so the formaldehyde penetrates the tiny sample very quickly with fast initial fixation and then glutaraldehyde gets in more slowly but gives more extensive stronger fixation. Am I wrong-will the formaldehyde initially harden the sample so the glutaraldehyde may not get in ?. Should I use the lowest formaldehyde concentration that will give initial fixation ( say 8% which will end up as 4% when diluted with equal amounts of glut.). Please let me know what you think. Regards Peter -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Wednesday, 31 December 2014 12:51 AM To: 'Peter Noyce' Subject: RE: [Histonet] formaldehyde concentration Peter, While 20% sounds like it should be faster it is not. It hardens the outside of the tissue quickly and can slow penetration to the remainder of the tissue. We use 4% which if you start full strength liquid formaldehyde actually with 1 part formaldehyde and 9 parts buffer or water is 3.7% that we can 10% formalin. It is a real issue as we are basing the 10% number on the amount of formaldehyde (37 - 40%) added to buffer or water not the actual amounts. Sorry it is confusing about amounts. Let me know if you need more information or this sounds dodgy. Pam -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Peter Noyce Sent: Monday, December 29, 2014 6:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] formaldehyde concentration I am using pure formaldehyde with no methanol and unbuffered as a first fixative. It comes in concentrations from 20% to 4%. From my reading; 1)it seems the higher strength penetrates faster ?. 2)4% is the minimum strength to get good penetration ?. Peter Noyce PhD student Australia. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 'Help'
Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
