[Histonet] Re: acid fast control tissue
Best acid fast control tissue I ever saw came from rhesus monkeys imported from India for research in the 1960s. A lot of them had active tuberculosis and were put down and autopsied (by the veterinary pathologists at Johns Hopkins). This was human tuberculosis, from tissue very similar to human lung. There must be some way to get this material today. Something I've long wanted to know: does Mycobacterium avium-intracellulare (MAI) infected tissue have the same acid-fast staining properties as Myco. tuberculosis? - We all know that Myco. leprae doesn't. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for 2 Gram pos/neg blocks
Hello All, I'm almost out of my gram pos/neg block. Would anyone be willing to trade for other types of tissue? I have AFB, iron, copper, amyloid. Thank you Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com ha...@histologistics.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cutting honey bees
Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting honey bees
I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP! Need some old fashioned histology advice
First, the very best of holidays to everyone. Now for the histology part. Our lab's focus is on the early stages of Alzheimer's Disease in the Brainstem using celloidin processing embedding for IHC staining. This year, our lab will be receiving 6 post-mortem whole human brains (1 every other month). After fixation, processing celloidin embedding, the whole brain will be serially cut at 100um thick. Each brain section will be 5 inches x 4.5 inches in size. I will given 250 of these whole brain sections to stain for tau IHC...that's 1500 whole brain sections/year!!! 1) Does anyone have experience doing manual IHC staining of large free-floating brain sections? 2) What type of staining tools, dishes or other essential equipment can anyone recommend? 3) What's the most efficient way to stain 250 sections for batch IHC staining - such as transferring batch sections (maybe 5-10) from reagent to reagent? 4) What type of batch apparatus to use? As for the antibody ABC steps, I was thinking of placing each section inside a large glass cigar tube (yep, people use large glass tubes with fitted cap to store cigars), with 5ml of antibody or ABC reagent gently agitate on a shaker/rotator at room temp during the incubation. Does anyone have ideas on this? Please, any ideas, suggestions or recommendation anyone can provide will be most greatly appreciated. Best regards Maria Mejia UCSF Department of Neurology San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
