Re: [Histonet] peach stone (pit) sectioning?
If you are just sectioning the (relatively softer) seed, then you might consider softening it in 4% EDT ethylene diamine, a.k.a. 1,2-diaminoethane. That _might_ work for the rather harder shell surrounding the seed, if you need to section that. Steaming the shell, or boiling it, night be necessary. You might find it worthwhile to consult _A Guide to Wood Microtomy_ by Ernie Ives (self-published, ISBN 0-9540551-0-1). A bit more information on your objective here would be helpful. Mike Andrews, W5EGO WWME Oklahoma area executive team On Feb 3, 2015, at 3:20 PM, Preiszner, Johanna preis...@mail.etsu.edu wrote: Hi Netters, does anybody know how I could soften up a hard fruit pit for sectioning? Thanks, Hanna Preiszner ETSU/QCOM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Know Error
We have many clients who perform Know Error for their patients. What we do is send out only one site with malignancy (preferably the highest grade) instead of all sites. This cuts down on a lot of unnecessary work to be done by the tech and cuts costs for you. I find it easiest and most cost effective to send them the vial of tissue instead of the slides. I also believe that if you contact a Know Error representative you should be able to develop a contract to ensure reimbursement. Hope this helps! Casey Arnold Histology Supervisor 1450 Elm Hill Pike Nashville, TN 37210 Office: 615.874.0410 Cell: 615.714.2940 Fax: 615.345.4595 carn...@opko.com www.opko.com NOTICE: This electronic mail message and any files transmitted with it are intended exclusively for the individual or entity to which it is addressed. The message, together with any attachment, may contain confidential and/or privileged information. Any unauthorized review, use, printing, saving, copying, disclosure or distribution is strictly prohibited. If you have received this message in error, please immediately advise the sender by reply email and delete all copies. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, February 02, 2015 12:11 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH (Marcum, Pamela A) 2. Know Error (Cartun, Richard) -- Message: 1 Date: Mon, 2 Feb 2015 14:22:58 + From: Marcum, Pamela A pamar...@uams.edu Subject: [Histonet] ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 56d5892a005f4deaaf2cfed127d97...@mail13m2n2.ad.uams.edu Content-Type: text/plain; charset=us-ascii Good Morning All, THE ARKANSAS SOCIETY FOR HISTOTECHNOLOGY - SPRING MEETING - MARCH 7TH, 2015 IN LITTLE ROCK, ARKANSAS PLEASE JOU US!! The meeting is being held at the Baptist Schools of Health in Little Rock and is open to anyone in Histology and related or interested fields. The following list is for the speakers, who will all be presenting 90 minute seminars for the meeting. We will have two rooms of speakers so you have a choice in classes and topics for the day. 7:30AM Registration 8AM to 9:30AM Seminars Dr Ericka Olgaard from UAMS Diagnostic Challenges of Breast Cancer Dr Daisy Alapat UAMS Methods and Basics of Protein Detection in Hematopathology 9:30AM to 10:00AM BREAK 10:00AM to 11:30AM Seminars Dr Shree Sharma Nephropath Integrating Mobile Technology With Anatomic Pathology Debbie Siena Technical Specialist Statlab Controlling Your Special Stains 11:30AM to 1:00PM Lunch 1:00PM to 2:30PM Seminars Dr Sara Shalin UAMS How and Why Skin Is Different Jennifer Feldman Ventana Antibody Production 2:30PM to 3:00PM Break 3:00PM to 4:30PM Seminars Dr Jennifer Forsythe ME Little Rock Autopsy in America Pamela Marcum HT UAMS Reagent Alcohol - Can't Drink It - What Is It? Thank You, Pam Marcum -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -- Message: 2 Date: Mon, 2 Feb 2015 16:52:40 + From: Cartun, Richard richard.car...@hhchealth.org Subject: [Histonet] Know Error To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 9215bd4b0ba1b44d962a71c758b68d2e3e0e2...@hhcexchmb03.hhcsystem.org Content-Type: text/plain; charset=us-ascii Our laboratory has a new urology group who is demanding that we send-out every Positive (with CA) prostate biopsy for the Know Error DNA identity test. The test is used to confirm the identity of the patient diagnosed with cancer by matching the DNA in the cancer cells to that obtained from a buccal swab
[Histonet] RE: Storage of paraffin blocks
To comply with Joint Commission standards, ours are stored 6 off the floor. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Tuesday, February 03, 2015 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage of paraffin blocks Hi everyone, I hope all is going well in the new year. I was wondering if everyone that stores their paraffin blocks in the plastic tissue tek block filing cabinets, stores these cabinets directly on the floor or do you all place them on some sort of stand? Thank you Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] time study on preparation of block and H E slide
Hi Histonetters, Has anyone has some information about time studies done of how long it takes to make a paraffin block and HE slide. Thanks Mala Holy Name Medical Center is ranked among the top hospitals in the nation for patient care, clinical performance and workplace excellence. Click here to learn more. Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problems with Universal Cassette Clamp
I have been using RM2255 for almost 10 years and never had a clamp problem. We have six in the lab with three of them more than 8 years old and other than routine PMs or the occasional human err problem these are great workhorses. We keep contracts on them so we have great service. I used one for about 4 years and cut plastic on it. The clamp was universal just not the same as the one on paraffin microtomes. Pam Marcum UAMS -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Rorick Sent: Tuesday, February 03, 2015 11:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with Universal Cassette Clamp Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS
Shane Jones will be presenting a one day class for HT Readiness for anyone who needs review for the exam this spring or early summer. The cost will be $60.00 for the day and includes lunch and breaks. Please let me know if you need further information about the class or if you would like to attend. Thank You, Pam Marcum ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problems with Universal Cassette Clamp
Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problems with Universal Cassette Clamp
I think we have had several break over the years... Is this where they say you have to buy the whole assembly for over $1000 or $1400 too?? We took a clamp off another older instrument. Rena Bouchal, M.S. Manager, Anatomic Pathology 304-293-7765 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Rorick Sent: Tuesday, February 03, 2015 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with Universal Cassette Clamp Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 135, Issue 2
Message-ID: D36CEBFE29A5AD4DA7CC6E2E7F8AF64442A129@SERV2012.aipathology.local Content-Type: text/plain; charset=us-ascii I am looking for some great programs for lean management in a laboratory setting (both cytology and histology). Can anyone help me out? Thank you, LeAnn Hi Leann, I reached out to our primary vendors (Leica, Ventana and Sakura) to come in and do a lean assessment. These companies provide free lean services you can ask for. Mary J. Leslie, BSMT(ASCP),MS(CPM) Manager, Core Laboratories Operations Tricore Reference Laboratories Office: 505-938-8409 Cellphone: 505-264-5506 mary.les...@tricore.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, February 03, 2015 11:01 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Know Error (Teri Johnson) 2. QC for IHC bulk solutions (Davis, Cassie) 3. Benchmark Special Stainers (Bitting, Angela K.) 4. Re: Benchmark Special Stainers (STACEY) 5. RE: Benchmark Special Stainers (Bitting, Angela K.) 6. Lean Management (LeAnn Lang) 7. QIHC (Algeo, Lacie A) 8. RE: QIHC (Bea DeBrosse-Serra) 9. RE: Know Error (Cesar Francisco Romero) 10. Storage of paraffin blocks (Fawn Bomar) 11. RE: Storage of paraffin blocks (Rathborne, Toni) 12. RE: Know Error (Casey Arnold) 13. ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS (Pam Marcum) 14. Problems with Universal Cassette Clamp (Anna Rorick) -- Message: 1 Date: Mon, 2 Feb 2015 18:13:33 + From: Teri Johnson tejohn...@genoptix.com Subject: [Histonet] RE: Know Error To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: f418ac77cd7e4a61954fa6e75b7f3...@phuscb-sp37mb03.genoptix.org Content-Type: text/plain; charset=us-ascii That sounds like a valid concern, Richard. Thank you for being an advocate for good utilization of patient testing. What is behind their concern? Do they think there has been a mistake with patient identification in your lab? Or are they trying to reconcile low PSA or other discordant screening test results with a diagnosis of prostate CA? I would offer to send whatever they need from the lab (paraffin section curls, unstained slides, etc) back to their office, so long as they provide patient consent. The urologist's office can then package that material with the buccal swabs they get from the patient. The onus can be on them to manage the cost of the testing, and they can then try to justify it with the patient when insurance will not reimburse for it. Is this option feasible? Teri CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. -- Message: 2 Date: Mon, 2 Feb 2015 13:24:02 -0500 From: Davis, Cassie cda...@che-east.org Subject: [Histonet] QC for IHC bulk solutions To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: be7af02009c3a74f9b8344e70f3655cb16a438d...@chexcms01.one.ads.che.org Content-Type: text/plain; charset=iso-8859-1 Good afternoon Histoland folks, other than documenting the ph of each Ventana bulk solution after diluting and visual inspections of slides run with that solution lot what other way is there to QC the EZ Prep, Reaction Buffer, and Liquid Coverslip bulks solutions? (we currently have a lively dialog going on about this) There is always something to do and something new in Histology... Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended
[Histonet] ThermoShandon Gemini AS Stainer w/ heat
Hello everyone, My director has asked if I can find the average lifespan of this stainer, and your experience. We are trying to justify a new stainer. Many thanks, Pam Pamela S. Younes MHS, HTL(ASCP), CPC, PA(ASCP) Assistant Professor UT Medical School Houston, Department of Pathology and Laboratory Medicine 6431 Fannin, MSB 2.136, Houston, TX 77030 (713) 566-5046 pamela.s.you...@uth.tmc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problems with Universal Cassette Clamp
Yes, unfortunately I am very familiar with this problem. We have probably had at least 10 clamp handles break off over the last 3 years. The problem is the soft metal they are using for the clamp post. The post fits into a hole in the bottom portion of the clamp and that is actually what pulls the clamp open so that you can insert a cassette. I have talked to our Leica rep numerous times about this issue. They first tried to blame our techs for the problem (they must be using it too aggressively) but they eventually took a limited amount of ownership for the inferior materials. At one point they thickened the clamp handle itself but the real problem is that post at the end of the handle. I have suggested that they make the handle (or at least the post) out of titanium or some other material that can stand up to the stress put on it by the clamp. I have not seen any additional improvements over the past two years and we still have clamp handles break off on occasion. Your Leica rep is well aware of this problem so contact them and ask for some new clamps (they will hopefully offer a free one or discounted one). I try to keep a couple of new spare clamps on hand as I have yet to figure out a way to repair that handle once it breaks off. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Rorick Sent: Tuesday, February 03, 2015 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Problems with Universal Cassette Clamp Hi Everyone, I am using a Leica RM2255 microtome with a universal cassette clamp. Two of the clamps have broken over the past year. Has anyone else had a problem with these? Anna Rorick Anatomic Pathology Technician Battelle Memorial Institute Columbus, Ohio Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Know Error
This may sound like a silly question, Rich, (or maybe not), but how do you know the buccal swab is properly labeled? My experience is that when there's a mix-up in patients' tissue, it often occurs in the doctor's office. A lot of pathology labs are barcoding, but all the tracking in the world can't prevent a mix up at the doctor's office. How are they ensuring that their DNA swabs haven't gotten switched? Sounds like frivolous spending to me...and where is the line drawn? When are we sending out DNA testing on all specimens, not just prostate biopsies? Good luck! Clare Clare J. Thornton, HTL(ASCP),QIHC Lead Immunohistochemistry Tech Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, February 03, 2015 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 135, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://cp.mcafee.com/d/5fHCNASyMCCqenD7PhOedTdETpo7fKcKfCQrII3DT66rCQrIecKc9T76QrII3xOqab9KQOtoMDm0agQCvoDJfVsSxFc-NfqvOVJRx2Wa33_nVUsCUPRXB-h7nppoVqWdAklrEEYG7DR8OJMddECPhOrKrKr01DOFeDdPFlKpcNZ9_67uwTwCHIcfBisEeRMDaAWwQSjBitgqn8lrxrW0E-l9QVKtaJP9CfFfUMXQ6PqpEVsvKr2YGjG3jh07i8_io1Cy0cRfd42bbU1Cy0dFhLPh0CrppdWvc9jYdQ or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Know Error (Teri Johnson) 2. QC for IHC bulk solutions (Davis, Cassie) 3. Benchmark Special Stainers (Bitting, Angela K.) 4. Re: Benchmark Special Stainers (STACEY) 5. RE: Benchmark Special Stainers (Bitting, Angela K.) 6. Lean Management (LeAnn Lang) 7. QIHC (Algeo, Lacie A) 8. RE: QIHC (Bea DeBrosse-Serra) 9. RE: Know Error (Cesar Francisco Romero) 10. Storage of paraffin blocks (Fawn Bomar) 11. RE: Storage of paraffin blocks (Rathborne, Toni) 12. RE: Know Error (Casey Arnold) 13. ARKANSAS SPRING MEETING INCLUDES 1 DAY HT READINESS (Pam Marcum) 14. Problems with Universal Cassette Clamp (Anna Rorick) -- Message: 1 Date: Mon, 2 Feb 2015 18:13:33 + From: Teri Johnson tejohn...@genoptix.com Subject: [Histonet] RE: Know Error To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: f418ac77cd7e4a61954fa6e75b7f3...@phuscb-sp37mb03.genoptix.org Content-Type: text/plain; charset=us-ascii That sounds like a valid concern, Richard. Thank you for being an advocate for good utilization of patient testing. What is behind their concern? Do they think there has been a mistake with patient identification in your lab? Or are they trying to reconcile low PSA or other discordant screening test results with a diagnosis of prostate CA? I would offer to send whatever they need from the lab (paraffin section curls, unstained slides, etc) back to their office, so long as they provide patient consent. The urologist's office can then package that material with the buccal swabs they get from the patient. The onus can be on them to manage the cost of the testing, and they can then try to justify it with the patient when insurance will not reimburse for it. Is this option feasible? Teri CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. -- Message: 2 Date: Mon, 2 Feb 2015 13:24:02 -0500 From: Davis, Cassie cda...@che-east.org Subject: [Histonet] QC for IHC bulk solutions To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: be7af02009c3a74f9b8344e70f3655cb16a438d...@chexcms01.one.ads.che.org Content-Type: text/plain; charset=iso-8859-1 Good afternoon Histoland folks, other than documenting the ph of each Ventana bulk solution after diluting and visual inspections of slides run with that solution lot what other way is there to QC the EZ Prep, Reaction Buffer, and Liquid Coverslip bulks solutions? (we currently have a lively dialog going on about this) There is always something to do and something new in
[Histonet] C1q FITC
Does anyone have a vendor with C1q FITC availability? We typically order Dako product #F0254 which is a polyclonal rabbit anti-human C1q complement, but are told they are on backorder for an undetermined amount of time (possibly May 2015). Thanks for your help! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: C1q FITC
http://www.ventana.com/product/180?type=174 http://www.thermoscientific.com/content/tfs/en/product/c1q-complement-fitc-labeled-antibody.html http://www.abcam.com/c1q-antibody-fitc-ab4223.html Walter Benton HT(ASCP)QIHC Lab Operations Manager Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) Chesapeakeurology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laudon, Heather M Sent: Tuesday, February 03, 2015 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C1q FITC Does anyone have a vendor with C1q FITC availability? We typically order Dako product #F0254 which is a polyclonal rabbit anti-human C1q complement, but are told they are on backorder for an undetermined amount of time (possibly May 2015). Thanks for your help! Heather Laudon, BS, HTL (ASCP)cm Histology Technician Surgical Pathology Laboratory Gundersen Health System 1900 South Avenue Lacrosse, WI 54601 Mail Stop: H04-008 (608)775-3139 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Know Error
Most problems are with the physician offices, not the lab, because they have bad practices like pre-labeling specimens. How does this fix that problem? You could have a buccal specimen and biopsy that match up fine but are on the wrong patient. Seems like collecting a buccal smear just adds even more variables and opportunities for error. Spend that time properly labeling the ACTUAL specimen(s) and problem solved. There is at least one lab that is offering Know Error testing on abnormal pap tests believe it or not. I didn't realize an abnormal pap test lead immediately to a hysterectomy.. http://manhattanlabs.com/for-doctors/mypap/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] best practices for HIER in electric pressure cookers
Hi Histonetters, I am doing some research into HIER in electric pressure cookers, are there any known CAP/CLIA regs about the cooker itself? Also what is the consensus on best practices for using electric pressure cooker these are my questions: should the amount of slides be standardized per run? should the amount of water each run be standardized how about the amount of containers each run? Temperature strips required each run? How about releasing the steam early vs letting pressure subside by itself prior to opening? Should HIER times be optimized as well as different pH solutions, for example should I test at different time intervals per retrieval solution for best results? Sorry for all the questions but it has been awhile since I have done manual retrieval so just want to find out what the guru's are saying. Thanks in advance, if you wish to reply off line that is good and I will publish the final results to the histonet. Thanks again Debbie Siena 800.442.3573 ext. 229 | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Know Error
This sounds like marketing scheme directly to the clinicians and expecting the lab to assume the reponsiblity for the costs. Confidentiality Statement: This email and any files transmitted with it may contain confidential and/or privileged material and is intended only for the person or entity to which it is addressed. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you have received this email in error, please notify the sender immediately and delete this material from all known records. Thank you Confidentiality Statement: This email and any files transmitted with it may contain confidential and/or privileged material and is intended only for the person or entity to which it is addressed. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you have received this email in error, please notify the sender immediately and delete this material from all known records. Thank you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webster, Thomas S. Sent: Tuesday, February 03, 2015 2:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Know Error Most problems are with the physician offices, not the lab, because they have bad practices like pre-labeling specimens. How does this fix that problem? You could have a buccal specimen and biopsy that match up fine but are on the wrong patient. Seems like collecting a buccal smear just adds even more variables and opportunities for error. Spend that time properly labeling the ACTUAL specimen(s) and problem solved. There is at least one lab that is offering Know Error testing on abnormal pap tests believe it or not. I didn't realize an abnormal pap test lead immediately to a hysterectomy.. http://manhattanlabs.com/for-doctors/mypap/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Know Error
I am not disagreeing nor am I sticking up for the company and not sure I'd even agree with the company but I think there is much more to this, from what little I know of them, than just mixing up two specimens at a physicians office. I believe their point of view of the company is besides patient ID errors that in making a cancer diagnosis, that the cancer material came EXCLUSIVELY from that one patient and is not a contaminant from another real cancer patient. Think floaters upon cutting, think leftover friable material from an embedding well that ends up in a cassette because embedder didn't clean forceps. You PCR up a V600E BRAF mutation from a melanoma slide and tube and how do you know 100% it is not DNA from a different patient who really needs the therapeutic but the first patient doesn't. Is the mutation really representative of the first patient or just a contaminant from the second? Again, I truly wonder about this being useful but you have to admit, there is more than only patient mis-identification but there are floaters and contaminations that must be paid attention to. Just my opinion. Ray Seattle, WA - Original Message - From: Thomas S. Webster twebs...@crh.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, February 3, 2015 11:52:13 AM Subject: [Histonet] RE: Know Error Most problems are with the physician offices, not the lab, because they have bad practices like pre-labeling specimens. How does this fix that problem? You could have a buccal specimen and biopsy that match up fine but are on the wrong patient. Seems like collecting a buccal smear just adds even more variables and opportunities for error. Spend that time properly labeling the ACTUAL specimen(s) and problem solved. There is at least one lab that is offering Know Error testing on abnormal pap tests believe it or not. I didn't realize an abnormal pap test lead immediately to a hysterectomy.. http://manhattanlabs.com/for-doctors/mypap/ CONFIDENTIALITY NOTICE: This e-mail message, including all attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. You may NOT use, disclose, copy or disseminate this information. If you are not the intended recipient, please contact the sender by reply e-mail immediately. Please destroy all copies of the original message and all attachments. Your cooperation is greatly appreciated. Columbus Regional Hospital 2400 East 17th Street Columbus, Indiana 47201___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FW: Iron control tissue or blocks
Spleen is a good, sensitive control to use for iron. From: Montana, Maria mtmont...@primecare.org To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 01/20/2015 08:39 AM Subject:[Histonet] FW: Iron control tissue or blocks Sent by:histonet-boun...@lists.utsouthwestern.edu From: Montana, Maria Sent: Wednesday, January 07, 2015 1:50 PM To: hiso...@lists.utsouthwestern.edu Subject: Iron control tissue or blocks Hi, Histoneters, I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested. Thanks, Maria Montana HTL (ASCP) CHI St Alexius Health (701)530-6732 This email may include confidential and privileged information. If this is not intended for your use, please destroy immediately and contact the sender of the message. - This message was secured by ZixCorp(R). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] peach stone (pit) sectioning?
Hi Netters, does anybody know how I could soften up a hard fruit pit for sectioning? Thanks, Hanna Preiszner ETSU/QCOM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
