[Histonet] RE: cleaning glassware
Also check the gelatine - is it a new batch? From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Anne Murvosh [amurv...@advancederm.net] Sent: Friday, 3 April 2015 6:01 AM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C3d?
Indirect IF Joelle Weaver MAOM, HTL (ASCP) QIHC From: lseb...@uwhealth.org To: pat...@gmail.com; histonet@lists.utsouthwestern.edu Date: Thu, 2 Apr 2015 12:25:05 + Subject: RE: [Histonet] C3d? CC: Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
We always acid clean before doing any silver stain. No metal Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Thursday, April 02, 2015 2:36 PM To: Jeff Halstead; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: cleaning glassware I work in a small veterinary lab and when I do the Warthin Starry stain I mix all the reagents in never used before disposable plastic beakers and I stain the slides in an un-used slide mailer. Roberta Horner Animal Diagnostic Lab Penn State University -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 2:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: TRAP staining on formic acid decalcified bone reference
The reference within a reference from Ray is A Chimeric Form of Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1β, TNF-α, PTH, PTHrP, and 1,25(OH)2D3 . Sean Morony et al . J Bone Mineral Res V 14, pp 1478-1485. However, the formic acid decalcification method is not described in detail and merely says formic acid but whether this is buffered formic acid or just dilute formic acid in water only is not stated. Ray might elaborate on what specific formic acid recipe he used as many in research don't always use buffered formic acid decalcifiying solutions. I would assume Morony et all used a buffered formic acid with either sodium formate or sodium citrate and controlled so as to not overexpose TRAP to acids longer than necessary. One publication, i.e., Eggert and Germain. Stable Acid Phosphatase I. Demonstration and Distribution. Histochem 66, pp 301-317, 1980) discussed in detail the rapid demineralization in acidic buffers i.e. buffered formic acid for staining of stable forms of acid phosphatase. I have both of these publications on file and will forward privately. I would err on the side of using a buffered formic acid with either sodium formate or sodium citrate for doing this and use decalcification endpoint testing to avoid over exposure to acid i.e. over decalcification. Take care Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Another question: C4d and preeclamptic placentas
Hello Netters, plus Matt and Keith ;-) I'm picking the collective brain of the Histonet... I've found some literature that suggests that up to 50% of preeclamptic placentas that resulted in a negative outcome have diffuse C4d staining when using IHC. Has anyone tried C4d IHC or IF on preeclamptic placentas? Tomorrow's Fridayso have a nice weekend everyone! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: TRAP staining on formic acid decalcified bone reference
Hi all, as usual Gayle was right on. Use a buffered (more gentle) formic acid; not just formic acid per se of any water diluted concentration. For end point testing, critical, we used a radiograph machine instead of chemical endpoints which is also fine;we just had access to a lot of equipment. Ray Washington - Original Message - From: Gayle Callis gayle.cal...@bresnan.net To: Histonet histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2015 3:12:55 PM Subject: [Histonet] Re: TRAP staining on formic acid decalcified bone reference The reference within a reference from Ray is A Chimeric Form of Osteoprotegerin Inhibits Hypercalcemia and Bone Resorption Induced by IL-1β, TNF-α, PTH, PTHrP, and 1,25(OH)2D3 . Sean Morony et al . J Bone Mineral Res V 14, pp 1478-1485. However, the formic acid decalcification method is not described in detail and merely says formic acid but whether this is buffered formic acid or just dilute formic acid in water only is not stated. Ray might elaborate on what specific formic acid recipe he used as many in research don't always use buffered formic acid decalcifiying solutions. I would assume Morony et all used a buffered formic acid with either sodium formate or sodium citrate and controlled so as to not overexpose TRAP to acids longer than necessary. One publication, i.e., Eggert and Germain. Stable Acid Phosphatase I. Demonstration and Distribution. Histochem 66, pp 301-317, 1980) discussed in detail the rapid demineralization in acidic buffers i.e. buffered formic acid for staining of stable forms of acid phosphatase. I have both of these publications on file and will forward privately. I would err on the side of using a buffered formic acid with either sodium formate or sodium citrate for doing this and use decalcification endpoint testing to avoid over exposure to acid i.e. over decalcification. Take care Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C3d?
Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: cleaning glassware
I always used to rinse the containers I used with alcohol and let them dry before doing the stain. Some people use an acid alcohol rinse. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeff Halstead Sent: Thursday, April 02, 2015 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cleaning glassware Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] Need Antibody (Bartonella/ Cat-Scratch) update
Hi Sheila- I received a reply from BioCare so I am passing the information along. Jeff. -Original Message- From: Dax Arguello [mailto:dargue...@biocare.net] Sent: Thursday, April 02, 2015 11:46 AM To: Jeffrey Robinson Subject: RE: [Histonet] Need Antibody Jeff, We do have the Bartonella Henselea antibody listed under Cat Scratch if you wanted to pass that info on to Sheila. It is now IVD as well. http://biocare.net/product/cat-scratch-antibody/ Best regards, Dax Arguello Account Executive Biocare Medical, LLC Mobile: (925) 586-2591 Fax: (925) 603-8080 E-mail: dargue...@biocare.net www.biocare.net ٠4040 Pike Lane٠Concord, CA 94520٠(800) 799-9499 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Thursday, April 02, 2015 11:40 AM To: Sheila Fonner; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Need Antibody Hi Sheila- I received some information from BioCare last year that they were releasing a Bartonella (ASR) antibody. I just checked their website and I did not see it listed but you might want to check with them (www.biocare.net). Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, April 02, 2015 10:48 AM To: Histonet Subject: [Histonet] Need Antibody Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about Formic acid decal and TRAP stain
Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsi...@statlab.com mailto:bbro...@statlab.com%7C | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] interface between Bond and Cerner CoPath Plus
Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? How does it work? Cost? Thank you Nancy Schmitt MLT, HT(ASCP) NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Question about Formic acid decal and TRAP stain
Debbie In our hands it has not worked on formic acid decaled samples, EDTA decal only. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Debra Siena Sent: Thursday, April 02, 2015 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsi...@statlab.com mailto:bbro...@statlab.com%7C | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Paraffin Waste Disposal
Hello All, I know each state's EPA laws regulate what can be done with the waste paraffin generated during embedding and processing, but I was wondering: how do you dispose of your paraffin waste? Ours is thrown away in biohazard bags and incinerated, though by our state law (OH), paraffin with fixed tissue can be thrown in the regular trash as long as no additives qualify it as a hazardous waste. We are performing a cost analysis and investigating the more green options that exist for paraffin disposal/recycling - I thought a poll of all you histo lab experts would be a great starting point. Thank you for your help! -Kelsey === Please consider the environment before printing this e-mail Cleveland Clinic is ranked as one of the top hospitals in America by U.S.News World Report (2014). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Deb - TRAP stain
I agree with Liz. EDTA. -- Vicki L. Kalscheur School of Veterinary Medicine Comparative Orthopaedic Research Laboratory 2015 Linden Drive Madison, WI 53706-1100 Phone: 608-262-8534 kalsc...@vetmed.wisc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Question about Formic acid decal and TRAP stain
In our hands we have never had TRAP success on Formic acid decalcified tissue. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, April 02, 2015 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 137, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=AwIDaQc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLEs=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzge= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Allowable temperature range (Linda Prasad (SCHN)) 2. Positive Control for IF? (Paula Sicurello) 3. C3d? (Paula Sicurello) 4. Re: Positive Control for IF? (Mark Tarango) 5. Re: Positive Control for IF? (Jim Burchette) 6. RE: C3d? (Sebree Linda A) 7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt) 8. Question about Formic acid decal and TRAP stain (Debra Siena) 9. RE: Question about Formic acid decal and TRAP stain (Elizabeth Chlipala) -- Message: 1 Date: Wed, 1 Apr 2015 22:39:00 + From: Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au Subject: RE: [Histonet] Allowable temperature range To: 'Tim H' thiggin...@msn.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1217ddb3d7de5e418e3d560a268eabd0e0e88...@xmdb03.nch.kids Content-Type: text/plain; charset=utf-8 If it's in formalin it can just stay at room temperature. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.schn.health.nsw.gov.aud=AwIDaQc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLEs=9Qb6_bEDCJKheqNhwxTb8wBpygzmxDAIDXa9byoCD2ke= Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim H Sent: Thursday, 2 April 2015 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Allowable temperature range What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=AwIDaQc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLEs=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzge= * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * -- Message: 2 Date: Wed, 1 Apr 2015 16:12:22 -0700 From: Paula Sicurello pat...@gmail.com Subject: [Histonet] Positive Control for IF? To: HistoNet histonet@lists.utsouthwestern.edu Message-ID: CAPSjddaNxLOoPhtQ0y4=ahu2qg7nnzgbhwfwomfvfyfngym...@mail.gmail.com Content-Type:
Re: [Histonet] RE:Question about Formic acid decal and TRAP stain
Debra, it appears most of the histology world disagrees with me but I stand by my post. If TRAP didn't work with formic acid in our hands, a major pharmaceutical treatment wouldn't be ready to help women with post-menopausal osteoporosis. Ray - Original Message - From: Sarah Mack sarah_m...@urmc.rochester.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2015 10:21:21 AM Subject: [Histonet] RE:Question about Formic acid decal and TRAP stain In our hands we have never had TRAP success on Formic acid decalcified tissue. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, April 02, 2015 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 137, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=AwIDaQc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLEs=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzge= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Allowable temperature range (Linda Prasad (SCHN)) 2. Positive Control for IF? (Paula Sicurello) 3. C3d? (Paula Sicurello) 4. Re: Positive Control for IF? (Mark Tarango) 5. Re: Positive Control for IF? (Jim Burchette) 6. RE: C3d? (Sebree Linda A) 7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt) 8. Question about Formic acid decal and TRAP stain (Debra Siena) 9. RE: Question about Formic acid decal and TRAP stain (Elizabeth Chlipala) -- Message: 1 Date: Wed, 1 Apr 2015 22:39:00 + From: Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au Subject: RE: [Histonet] Allowable temperature range To: 'Tim H' thiggin...@msn.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 1217ddb3d7de5e418e3d560a268eabd0e0e88...@xmdb03.nch.kids Content-Type: text/plain; charset=utf-8 If it's in formalin it can just stay at room temperature. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.schn.health.nsw.gov.aud=AwIDaQc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLEs=9Qb6_bEDCJKheqNhwxTb8wBpygzmxDAIDXa9byoCD2ke= Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim H Sent: Thursday, 2 April 2015 1:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Allowable temperature range What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage? Any ideas? Tim ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=AwIDaQc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLEs=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzge= * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were
Re: [Histonet] Question about Formic acid decal and TRAP stain
Hi Debra, My experience differs from Elizabeth and others. Indeed have made thousands of mouse bone preparations with formic acid decaled sections. Here is an article that didn't copy paste so well: RANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolism Ji Li*, Ildiko Sarosi † , Xiao-Qiang Yan † , Sean Morony † , Casey Capparelli † , Hong-Lin Tan † , Susan McCabe*, Robin Elliott*, Sheila Scully Gwyneth Van † , Stephen Kaufman † , Shao-Chieh Juan † , Yu Sun † , John Tarpley † , Laura Martin † , Kathleen Christensen James McCabe † , Paul Kostenuik † , Hailing Hsu*, Frederick Fletcher † , Colin R. Dunstan † , David L. Lacey , and William J. Boyle* Departments of *Cell Biology and Pathology, Amgen Inc., One Amgen Center Drive, Thousand Oaks, CA 91320 Edited by David V. Goeddel, Tularik, Inc., South San Francisco, CA, and approved December 20, 1999 (received for review September 30, 1999) PNAS u February 15, 2000 u vol. 97 u no. 4 u 1571 Is in PNAS and while the methods are not here in this article, look in the reference section to materials and methods used and see beautiful pictures of TRAP from formic acid decaled bones. I did them for years after that. Complete fixation and gentle formic acid (or immunocal) and indeed this can be done. Ray (retired in Lake Forest Park, WA, golf and science education outreach for K-12 busy) - Original Message - From: Debra Siena dsi...@statlab.com To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 2, 2015 9:00:25 AM Subject: [Histonet] Question about Formic acid decal and TRAP stain Hi Histonetters, I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help. Debbie Siena dsi...@statlab.com mailto:bbro...@statlab.com%7C | www.statlab.comhttp://www.statlab.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need Antibody
http://www.novusbio.com/Bartonella-henselae-Antibody-H2A10_NB600-1208.html CN -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, April 02, 2015 10:48 AM To: Histonet Subject: [Histonet] Need Antibody Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need Antibody
Hi Sheila- I received some information from BioCare last year that they were releasing a Bartonella (ASR) antibody. I just checked their website and I did not see it listed but you might want to check with them (www.biocare.net). Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Fonner Sent: Thursday, April 02, 2015 10:48 AM To: Histonet Subject: [Histonet] Need Antibody Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cleaning glassware
Hi, All-I am currently experiencing problems with our warthin-stary silver stain. The finished product has silver particulates all over the empty slide and deposited on the tissue making the stain very difficult to interpet. Any ideas would be very helpful. thanx ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need Antibody
Good afternoon Histonetters! Does anyone out there know of a good antibody for Bartonella? I would appreciate any information you could give me. Thank you for your knowledge and willingness to share information. SheilaKnoxville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
