[Histonet] HT jobs in Eureka, CA
I have a graduate looking for histotechnician jobs in the Eureka, CA area. Please contact me if you know of any. Thanks, Jennifer MacDonald ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] B-gal positive control
Anna, Don't know if you are talking human or non-human control or if makes a difference and I don't want to get into that whole discussion again. But if a mouse control is OK, one of the cleanest and nicest systems I used for B-gal was to get a transgenic mouse, easily obtainable with a Tie-2/lacZ promoter/reporter. B-gal expressed only on vascular endothelium so your assay can easily be tweaked for strength and cleanliness of signal. Have all the frozen/FFPE blocks you could ever need. Ray, Lake Forest Park, WA - Original Message - From: Anna Coffey (NIH/NCI) [C] anna.cof...@nih.gov To: histonet@lists.utsouthwestern.edu Sent: Friday, May 8, 2015 7:50:42 AM Subject: [Histonet] B-gal positive control Hello Histonet, Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.cof...@nih.gov 301-846-1730 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday Trivia Question: Most specimen on a single case
227 blocks on a bone tumor. Resident grossed it of couse! It was a nightmare. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, May 08, 2015 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday Trivia Question: Most specimen on a single case
I did ask about specimen/container, but knowing block count is interesting as well. Guys, I'm just the LIS vendor and I'm just happy that our LIS can handle this. Since it's been asked, I have asked what 'kind' of specimen these are. Oh, and as far as billing is concerned, since this is hospital based, I believe the billing will based upon admitting icd code... Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: Young, Henry O III CIV USN NAVHOSP CLNC (US) [mailto:henry.o.young@mail.mil] Sent: Friday, May 08, 2015 10:19 AM To: Marcum, Pamela A; 'Michael Mihalik'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Trivia Question: Most specimen on a single case Read the question, it is a 100 containers on one case. Not block count. HY -Original Message- From: Marcum, Pamela A [mailto:pamar...@uams.edu] Sent: Friday, May 08, 2015 12:50 PM To: 'Michael Mihalik'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a single case 157 blocks on one breast case - resident grossed it. -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, May 08, 2015 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing question
From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: Vickroy, James [mailto:jvick...@springfieldclinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an A specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday Trivia Question: Most specimen on a single case
157 blocks on one breast case - resident grossed it. -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, May 08, 2015 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Friday Trivia Question: Most specimen on a single case
Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday Trivia Question: Most specimen on a single case
I've never seen that many on one case. The most I've seen is ~30 (parathyroid) and its been many years. What types of specimens are they? Stacy -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, May 08, 2015 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing question
This is incorrect. The response from Joyce Weems is correct. And yes, it's confusing to everyone. Special stains are billed per block IHC is billed per specimen So a GMS on blocks A1 and A2 is GMS charge x 2 If you are not charging special stains per block you are losing revenue you are entitled to for 2015 Vinnie Della Speranza | Manager for Anatomic Pathology Services| Medical University of South Carolina | 165 Ashley Avenue MSC 908 | Charleston, South Carolina 29425 | Office: 843.792.6353 | Fax: 843.792.8974 | del...@musc.edu -Original Message- From: Campbell, Tasha M. [mailto:tmcam...@fmh.org] Sent: Friday, May 08, 2015 7:51 AM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing question From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: Vickroy, James [mailto:jvick...@springfieldclinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an A specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com%3cmailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday Trivia Question: Most specimen on a single case
I once saw an entire breast submitted--it was something like 100+ blocks. Thanks, Brian -Original Message- From: Stacy McLaughlin [mailto:stacy_mclaugh...@cooley-dickinson.org] Sent: Friday, May 08, 2015 9:49 AM To: Michael Mihalik; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Trivia Question: Most specimen on a single case I've never seen that many on one case. The most I've seen is ~30 (parathyroid) and its been many years. What types of specimens are they? Stacy -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, May 08, 2015 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] B-gal positive control
Hello Histonet, Has anyone out there come across a good FFPE positive control for B-gal? If so, please let me know! We would like to purchase a block or unstained slides if at all possible. Thanks! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.cof...@nih.gov 301-846-1730 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Billing question
Special stains are charged per block. If you have blocks A1, A2, and A3, and do AFB and GMS on all three that would be 6 charges. Sh... this will probably change next year!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Campbell, Tasha M. [mailto:tmcam...@fmh.org] Sent: Friday, May 08, 2015 7:51 AM To: Vickroy, James; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Billing question From my understanding its just one charge. Its per specimen such as A, B, C, etc. Someone else said that it would be 2 charges so am I wrong on this?? If you did GMS on A1 and AFB on A2 then that would be 2 charges because its two different stains. Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: Vickroy, James [mailto:jvick...@springfieldclinic.com] Sent: Thursday, May 07, 2015 4:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing question If I have two sections on an A specimen A1 and A2 and both had a GMS stain, do I charge 1 - 88312 or 2 - 88312? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Friday Trivia Question: Most specimen on a single case
Blocks, yes. Parts of a case (specimens), no. -Original Message- From: Michael Mihalik [mailto:m...@pathview.com] Sent: Friday, May 08, 2015 9:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Trivia Question: Most specimen on a single case Please excuse the trivia query, but we've got a client who somewhat regularly creates cases with 100+ specimen. I think the most I have ever seen is 127. I'm curious how common this is. What's the most specimen on a single case you've ever seen? Thanks for your patience and experience. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology Lab Assistant / Medical Assistant
Acadiana Gastroenterology Associates' of Lafayette Louisiana is looking for a Part-time (25-30 hour/ week) Histology Lab Assistant for our small GI Laboratory. Hours are flexible and Monday thru Friday only. The Ideal candidate would have previous Laboratory Assistant experience in an Anatomical Pathology Laboratory. This should include working with reagents and chemicals, filing blocks, working with staining and coverslip equipment. Strong knowledge of Safety Regulations and OSHA Standards. Must have a good understanding of Medical Terminology and must have High School diploma or GED equivalent and must be legally employable in the state of Louisiana. Apply on line at careerbuilders.com. Brent Adams - BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Short Term travel Tech Agencies
Does anyone know of an agency that hires Histology Techs for short term assignments, less than 3 month assignments? Marsha Price Sent from my iPad On May 8, 2015, at 12:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Still having Issues with Acetone fixation. (Lewis, Patrick) 2. Re: Histonet Digest, Vol 138, Issue 9 (Myra Huth) 3. Final Week to Register for FSH (John Shelley) 4. Program in Interactive tissue microarray and quantitative digital pathology workshop announcement (Linda Margraf) 5. Billing question (Vickroy, James) 6. Re: Billing question (Weems, Joyce K.) 7. HT jobs in Eureka, CA (Jennifer MacDonald) 8. Re: Billing question (Campbell, Tasha M.) 9. B-gal positive control (Coffey, Anna (NIH/NCI) [C]) 10. Re: Billing question (della Speranza, Vinnie) 11. Re: B-gal positive control (koelli...@comcast.net) 12. Re: Billing question (Weems, Joyce K.) 13. Friday Trivia Question: Most specimen on a single case (Michael Mihalik) 14. Re: Friday Trivia Question: Most specimen on a single case (Stacy McLaughlin) 15. Re: Friday Trivia Question: Most specimen on a singlecase (Marcum, Pamela A) 16. Re: Friday Trivia Question: Most specimen on a singlecase (Cooper, Brian) 17. Re: Friday Trivia Question: Most specimen on a single case (Morken, Timothy) -- Message: 1 Date: Thu, 7 May 2015 17:01:46 + From: Lewis, Patrick patrick.le...@seattlechildrens.org To: (Histonet@lists.utsouthwestern.edu) Histonet@lists.utsouthwestern.edu,16S-CSF Study 16s-csfst...@seattlechildrens.org Subject: [Histonet] Still having Issues with Acetone fixation. Message-ID: 3903be18914f4440834f0e620415ffca3cb6d...@ppwexd01d.childrens.sea.kids Content-Type: text/plain; charset=us-ascii Hi everyone, Sorry to keep posting about this, But I am still having Acetone issues. I am doing IHC for Cell surface markers that are lost when fixing with etoh or methanol. When I fix in 100% acetone my epitopes have great signal. However, when I fix in 100% acetone, my tissues all damaged by the acetone beyond all recognition. I can lose up to 90% of my tissue when I fix in 100% acetone for 10 minutes. But, I get good epitope staining if I have some tissue left on the slide. When I fix in anything else, I lose 90% or more of my epitope staining, but my tissue morphology looks great. -- What's the least amount of time I can fix in 100% Acetone for a 5uM section and still have it be fixed? Is drying after the 100% acetone fixing essential? or Bad for protecting tissue morphology? -- I am doing IHC on human tonsils cut to 5 uM with a 24 dry after sectioning. When I fix in 100% acetone, I fix it at 4C for 10 minutes. Then dry for 1 hour in the fume hood Should it go straight into buffer? Should it be for less time in the acetone? Should the acetone be Room temp or -20C instead of 4C. If I was diluting the acetone with buffer, (or etoh) then I could see going straight into buffer afterwards, but because I am using 100% I think that going into liquid right after fixing is too much of a change and my tissues go BOOM. Please help. Patrick Lewis Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. -- Message: 2 Date: Thu, 7 May 2015 17:34:33 + From: Myra Huth mh...@baymedical.org To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 9 Message-ID: 6b3280e0cd2c394894743e076dc66c59e...@bmcmsexchmb2.corp.baymedical.org Content-Type: text/plain; charset=us-ascii For some reason histonet is being delivered blank. -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Thursday, May 07, 2015 12:00
[Histonet] 2015 CAP Inspection
Another successful AP CAP Inspection with zero discrepancies/zero recommendations! V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2015) USAF, MSgt (Retired) Anatomic Pathology Technical Supervisor 10th Medical Group 210-687-7540 Cell mailto:ian.bern...@comcast.net ian.bern...@comcast.net mailto:ian.bernar...@us.af.mil ian.bernar...@us.af.mil University of Alabama Birmingham- Alumni. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bleach as Cleaning Agent or Decontamination-
Our facility is moving towards standardization of decontaminants or disinfectants. They prefer all areas use a Sani wipes that kills most pathogens. However, we contend for Anatomic pathology we need our liquid bleach not only as disinfectant or decontaminant but as a cleaning agent for stained lab-ware. You thoughts? Also, what concentration of Bleach (5.25 or 10%) is acceptable for use as both a disinfectant and cleaning agent or should we keep them separate? We used to buy the hospital grade premade bleach at a 5.25% concentration but now they want us instead to buy the 99.9% commercial Bleach and dilute from there. Any suggestions on opaque containers for us to purchase since bleach break down after a time period, at least for disinfecting? V/r IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Bleach as Cleaning Agent or Decontamination-
You need to be clear about what the concentration means. Household bleach (such as Javex or Clorox) is a 5% aqueous solution of sodium hypochlorite (NaClO). Sometimes it also contains a polymer (not named on the label) to increase the viscosity. Solutions for adding to swimming pools are 10% aqueous sodium hypochlorite and are cheaper (per gm of NaClO) than household bleach. These pool disinfectants are often labelled liquid chlorine, a deceptive name that fosters ignorance among those who didn't pay attention at school. Chlorine boils at -34C at atmospheric pressure and exists as a liquid at ambient temperatures only when compressed in railroad tanks or in the (much smaller) gas shells used as weapons by both sides in the First World War. Dakin's solution, used for cleaning dirty wounds, is 0.45-0.50% sodium hypochlorite, made by dilution with a carbonate-bicarbonate buffer to reduce alkalinity. A stronger solution could be used for non-living surfaces. Aqueous solutions of NaClO are remarkably stable but the solid compound is extremely unstable. The crystaline pentahydrate melts at 18C and is decomposed by reaction with carbon dioxide from the air;. Anhydrous NaClO is obtainable only by freeze-drying and is very explosive. Nobody uses the solid. The solutions are made by reaction of chlorine (gas) with aqueous sodium hydroxide solutions. This can also be done by electrolysis of a sodium chloride solution between inert electrodes. My sources of information are bleach bottle labels (small print) and the Merck Index 12th edition, 1996, checked today. Every lab should have a Merck Index on the shelf of reference books! The phrase 99.9% commercial bleach could not possibly mean 99.9% of either NaOCl or its pentahydrate (solubility 29%). 5.25% (absurd precision!) and 10% bleach probably mean volume dilutions (1:20 or 1:10) of a household hypochlorite bleach without added thickener. A swimming pool 10% sodium hypochlorite solution probably is the cheapest source of chlorine bleach disinfectant without unwanted additives. Dilute it 10-20X with water to swab your possibly infected surfaces. Beware of lab supply companies selling household products at greatly inflated prices. Anyone purporting to sell hospital grade premade bleach needs to be viewed with much suspicion. Think before you buy. John Kiernan London, Canada = = = On 08/05/15, ian bernard ian.bern...@comcast.net wrote: Our facility is moving towards standardization of decontaminants or disinfectants. They prefer all areas use a Sani wipes that kills most pathogens. However, we contend for Anatomic pathology we need our liquid bleach not only as disinfectant or decontaminant but as a cleaning agent for stained lab-ware. You thoughts? Also, what concentration of Bleach (5.25 or 10%) is acceptable for use as both a disinfectant and cleaning agent or should we keep them separate? We used to buy the hospital grade premade bleach at a 5.25% concentration but now they want us instead to buy the 99.9% commercial Bleach and dilute from there. Any suggestions on opaque containers for us to purchase since bleach break down after a time period, at least for disinfecting? V/r IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
