[Histonet] THICK AND THIN SECTIONS ?
Do you have one of the following microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA/JUNG 2035 LEICA 2025 LEICA 2030 Biocut LEICARM 2125/2125 RT LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome. ( No parts availability ) Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. Klaus Dern Phone: 706 635-8840 E-Mail: klaus.der...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] dehydration time for a relatively large sample
Hi, Thanks for prompt response. We unfortunately do not have a processor in our lab at university..The protocol i wrote was working in quail embryonic samples (just before hatching). I process the tissue manually and cannot process the tissue with strict time schedule. Thus, I need to leave a sample overnight at some point. I cut the zebra finch head into anterior (beak) and posterior (brain) region and mid-sagittally in both. so each tissue sample is about 0.5 X 1 X 1cm cube at maximum. I have also tried short time schedule compared the one i wrote in previous email, for similar sized sample (zebra finch beak). However, it never worked. Thus i prolonged the each step for the latest sample. It would be great if you could provide me the proper time schedule. rui From: k...@regionsjaelland.dk To: ru...@hotmail.com Subject: SV: [Histonet] dehydration time for a relatively large sample Date: Wed, 20 May 2015 06:18:24 + Hi Rui Which processor unit do you use to me it seems like a wrong protocol. Maybe I can help you set up a better protocol - if you want - but then I need to know size og your tissue, processor unit and what kind of clearens you use. Kind regards Karen Supervisor tissue processing Denmark -Oprindelig meddelelse- Fra: Rui TAHARA [mailto:ru...@hotmail.com] Sendt: Wednesday, May 20, 2015 7:11 AM Til: histonet@lists.utsouthwestern.edu Emne: [Histonet] dehydration time for a relatively large sample Hi, I am wondering if prolong dehydration time with 95 and 100% ethanol would brittle the sample for paraffin sectioning. I have been trying to section adult zebra finch beak and processed several samples, however, i failed to obtain a good section. It appears that the paraffin did not penetrated the tissue. This may be derived from incomplete dehydration and clearing before paraffin. Because i have read somewhere that if the sample was sitting in 95 and 100% ethanol too long it would be brittle and be teared when sectioned. I have processed only a beak (about 1 cmX0.5 mm). Also when is an appropriate time to use vacuum? I am afraid that if i used it at 100% ethanol, the ethanol would evaporate so far I have tried; fix overnight decalcified few days walk from water to 70% ethanol; overnight 85%, 95% (2times change) and 100 % (2 times change) ethanol; overnight clearing; 2 days paraffin (2 times change); overnight Any suggestion would be appreciated. Thank you in advance, rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Florida NSH members
Hello Florida NSH Histonetters, I am in need of those who would like to be part of the NSH House of Delegates for the Florida Society. We are entitled to a certain number delegates who are NSH active members from the state of Florida. The annual meeting of the House of Delegates of the National Society for Histotechnology will convene on September 2, 2015 at 7:00 PM in Washington, DC. I need to submit this information as soon as possible and would ask if you contact me off-line in response to this request. Thanks!!! Kind Regards! John J Shelley 2014-2016 FSH President ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] THICK AND THIN SECTIONS ?
I see that advertising is now allowed on the Histonet? James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: Klaus Dern [mailto:klaus.der...@gmail.com] Sent: Wednesday, May 20, 2015 6:16 AM To: histonet Subject: [Histonet] THICK AND THIN SECTIONS ? Do you have one of the following microtomes and the advance mechanism is worn out. ( too much play between spindle and spindlenut ) REICHERT/JUNG 2030 LEICA/JUNG 2035 LEICA 2025 LEICA 2030 Biocut LEICARM 2125/2125 RT LEICA - CM 1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome. ( No parts availability ) Rather than replacing these excellent Instruments, I have a PERMANENT solution to fix this problem. Klaus Dern Phone: 706 635-8840 E-Mail: klaus.der...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microscope Problems?
I see that advertising is now allowed on the Histonet? James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: scott munday [mailto:mundaysc...@gmavt.net] Sent: Tuesday, May 19, 2015 11:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microscope Problems? *We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. * *All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email with questions.* -- Munday Scientific Instrument Service 90 Misha Lane Sanford, NC 27330 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] training log
Hi, Actually that would be cool for comparison purposes for me too. Perhaps it might be easiest to put them on dropbox and post a link to the histonet. But please share. Thanks, Amos Brooks On Tue, May 19, 2015 at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 12 Date: Tue, 19 May 2015 14:42:23 + (UTC) From: Histology Technician histology81...@att.net To: Finlay Finlay finlay.fin...@glasgow.ac.uk, 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] training log Message-ID: 610982354.946524.1432046543103.javamail.ya...@mail.yahoo.com Content-Type: text/plain; charset=UTF-8 I'd be interested in this too if anyone is willing to share.Thanks! On Tuesday, May 19, 2015 9:03 AM, Finlay Finlay finlay.fin...@glasgow.ac.uk wrote: Hello all, Does anyone have a training log or competency list that they would be willing to share with me for an absolute beginner to hands on histology. I have previously trained people with limited experience but never from scratch (straight from university). I am keen not to miss the basic lab skills that I've previously taken for granted. Thank you in advance Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] What kind of undecalcified bone, mama or frozen, human or mouse?
Message: 1 Date: Tue, 19 May 2015 17:01:04 + From: Hernandez, Jesus Willibaldo hernande...@uthscsa.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: d49247f053184d45ba34bffee794d2fa26e5c...@ex-ctrc-mbx3.win.uthscsa.edu Content-Type: text/plain; charset=us-ascii Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. Regards, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 138, Issue 24
Where are the photos? Can we upload photo? On May 20, 2015, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Need help reviewing undecalcified bone slides (Hernandez, Jesus Willibaldo) 2. OJT Histotechs/Training (Coffey, Anna (NIH/NCI) [C]) 3. Microscope Problems? (scott munday) 4. Re: OJT Histotechs/Training (Elizabeth Chlipala) 5. Re: OJT Histotechs/Training (Loralei Dewe) 6. Re: training log (Joelle Weaver) 7. Co-path users logging Slides in (Histology Technician) 8. Re: Co-path users logging Slides in (Weems, Joyce K.) 9. Re: Co-path users logging Slides in (Sue) 10. Re: Co-path users logging Slides in (Whitaker, Bonnie) 11. Re: Co-path users logging Slides in (Morken, Timothy) 12. dehydration time for a relatively large sample (Rui TAHARA) 13. THICK AND THIN SECTIONS ? (Klaus Dern) 14. Re: dehydration time for a relatively large sample (Rui TAHARA) 15. Re: THICK AND THIN SECTIONS ? (James Watson) 16. Re: Microscope Problems? (James Watson) -- Message: 1 Date: Tue, 19 May 2015 17:01:04 + From: Hernandez, Jesus Willibaldo hernande...@uthscsa.edu To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: d49247f053184d45ba34bffee794d2fa26e5c...@ex-ctrc-mbx3.win.uthscsa.edu Content-Type: text/plain; charset=us-ascii Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. Regards, ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Uploading images
The hyperlinks to Jesse's images got cut off in my email. If those links don't work, try these. Image 1 http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-8-micr ons/ Image 2 http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-5-micr ons-3/ Marvin -Original Message- From: mha...@histosearch.com [mailto:mha...@histosearch.com] Sent: Wednesday, May 20, 2015 10:19 PM To: abt...@gmail.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Uploading images You may upload images at http://histosearch.com/imageupload/ http://histosearch.com/imageupload/. Attachments are not allowed on the Histonet list server. The images on undecalcified bone sections that Jesse uploaded are at: http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-8-micr o http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-8-micro ns/ http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-5-micr o http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-5-micro ns-3/ Let me know if you have any questions or problems. Marvin -Original Message- From: mailto:abt...@gmail.com abt...@gmail.com [ mailto:abt...@gmail.com mailto:abt...@gmail.com] Sent: Wednesday, May 20, 2015 6:33 PM To: mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 24 Where are the photos? Can we upload photo? On May 20, 2015, at 1:00 PM, mailto:histonet-requ...@lists.utsouthwestern.edu mailto:histonet-requ...@lists.utsouthwestern.edu mailto:histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to mailto:histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to mailto:histonet-requ...@lists.utsouthwestern.edu mailto:histonet-requ...@lists.utsouthwestern.edu mailto:histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at mailto:histonet-ow...@lists.utsouthwestern.edu mailto:histonet-ow...@lists.utsouthwestern.edu mailto:histonet-ow...@lists.utsouthwestern.edu histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Need help reviewing undecalcified bone slides (Hernandez, Jesus Willibaldo) 2. OJT Histotechs/Training (Coffey, Anna (NIH/NCI) [C]) 3. Microscope Problems? (scott munday) 4. Re: OJT Histotechs/Training (Elizabeth Chlipala) 5. Re: OJT Histotechs/Training (Loralei Dewe) 6. Re: training log (Joelle Weaver) 7. Co-path users logging Slides in (Histology Technician) 8. Re: Co-path users logging Slides in (Weems, Joyce K.) 9. Re: Co-path users logging Slides in (Sue) 10. Re: Co-path users logging Slides in (Whitaker, Bonnie) 11. Re: Co-path users logging Slides in (Morken, Timothy) 12. dehydration time for a relatively large sample (Rui TAHARA) 13. THICK AND THIN SECTIONS ? (Klaus Dern) 14. Re: dehydration time for a relatively large sample (Rui TAHARA) 15. Re: THICK AND THIN SECTIONS ? (James Watson) 16. Re: Microscope Problems? (James Watson) -- Message: 1 Date: Tue, 19 May 2015 17:01:04 + From: Hernandez, Jesus Willibaldo mailto:hernande...@uthscsa.edu mailto:hernande...@uthscsa.edu mailto:hernande...@uthscsa.edu hernande...@uthscsa.edu To: mailto:histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: d49247f053184d45ba34bffee794d2fa26e5c...@ex-ctrc-mbx3.win.uthscsa.edu Content-Type: text/plain; charset=us-ascii Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is
Re: [Histonet] Uploading images
You may upload images at http://histosearch.com/imageupload/. Attachments are not allowed on the Histonet list server. The images on undecalcified bone sections that Jesse uploaded are at: http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-8-micro ns/ http://histosearch.com/imageupload/undecalcified-human-bone-he-stain-5-micro ns-3/ Let me know if you have any questions or problems. Marvin -Original Message- From: abt...@gmail.com [mailto:abt...@gmail.com] Sent: Wednesday, May 20, 2015 6:33 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 138, Issue 24 Where are the photos? Can we upload photo? On May 20, 2015, at 1:00 PM, mailto:histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to mailto:histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at mailto:histonet-ow...@lists.utsouthwestern.edu histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Need help reviewing undecalcified bone slides (Hernandez, Jesus Willibaldo) 2. OJT Histotechs/Training (Coffey, Anna (NIH/NCI) [C]) 3. Microscope Problems? (scott munday) 4. Re: OJT Histotechs/Training (Elizabeth Chlipala) 5. Re: OJT Histotechs/Training (Loralei Dewe) 6. Re: training log (Joelle Weaver) 7. Co-path users logging Slides in (Histology Technician) 8. Re: Co-path users logging Slides in (Weems, Joyce K.) 9. Re: Co-path users logging Slides in (Sue) 10. Re: Co-path users logging Slides in (Whitaker, Bonnie) 11. Re: Co-path users logging Slides in (Morken, Timothy) 12. dehydration time for a relatively large sample (Rui TAHARA) 13. THICK AND THIN SECTIONS ? (Klaus Dern) 14. Re: dehydration time for a relatively large sample (Rui TAHARA) 15. Re: THICK AND THIN SECTIONS ? (James Watson) 16. Re: Microscope Problems? (James Watson) -- Message: 1 Date: Tue, 19 May 2015 17:01:04 + From: Hernandez, Jesus Willibaldo mailto:hernande...@uthscsa.edu hernande...@uthscsa.edu To: mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Need help reviewing undecalcified bone slides Message-ID: d49247f053184d45ba34bffee794d2fa26e5c...@ex-ctrc-mbx3.win.uthscsa.edu Content-Type: text/plain; charset=us-ascii Hi all, I just wanted to know if anyone out there had experience reviewing undecalcified bone sections. I still feel like the morphology is not showing up on my sections so I am in need of assistance in troubleshooting this issue. I just uploaded two pictures on the histonet server for review. You can also reply to me for more pictures or my methodology. Thank you once again. Regards, ___ Histonet mailing list mailto:Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
