[Histonet] Searching for Horizontal Slide Racks
Hi Histonet- Does anyone have any slide racks that hold the slides horizontally that they no longer need? Like this but for maybe 50-60 slides? http://grale.com.au/products/view/297 I am willing to purchase and pay for shipping if anyone has any they are getting rid of. Thank you! Erin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Trichrome troubleshooting
Actually, I have microwaved the Bouins (and still do) for small numbers of slides and the results are the same. I ran a liver bx both ways as well as larger tissue to compare. I use the 10 slide plastic coplin jar and generally have 5 or less slides when I do this. One microwaves the Bouins for 30 seconds on power level 7 in a lab grade microwave. A higher level will cause the Bouin's to spill. Slides are then added and left for 5 minutes. Excess rinsed out and then proceed as per your SOP. As for the blue- I rinse out excess and do 1 dip in 1% GAA. Rinse and dehydrate (10 dips each solution) Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: Elizabeth Chlipala [mailto:l...@premierlab.com] Sent: Tuesday, June 30, 2015 3:30 PM To: Suzanne Martin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome troubleshooting Suzanne How many times have you used the kit and reagents, I did look up how the kit works but the trichrome stain can be tricky. First of all you need to make sure that the mordant (bouins solution) is at 60C prior to placing your slides in them. We normally heat up our bouins for at least an hour prior to placing the slides in the solution. We leave in bouins for an hour and a half rather than an hour. I see that this is a microwave protocol I cannot comment on that but I don't think that the hematoxylin is the issue, if you leave longer in 1% acetic acid that may pull some of the blue stain out or I would try dehydrating with lower alcohol percentage that can pull some of the blue stain out. I would also try leaving it a bit longer in the bouins after you microwave it - that might help. Trichrome staining works best with fresh reagents so if you have used these reagents too much that could cause problems. I'm also not a big fan of the one step trichromes, they are quicker but sometimes not as good as the two steps, just my opinion. FYI - to evaluate your staining look for a smaller vessel, the smooth muscle should be nice a red, if its greyish or blue you have not done the stain properly. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Suzanne Martin [mailto:smar...@lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Unsubscribe
Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email lea.almi...@tuhs.temple.edu This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. You can direct questions concerning PHI or HIPAA to the Corporate Compliance and Privacy Officer at (215) 707-5605. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Trichrome troubleshooting
Please clarify if you are referring to the Aniline blue, or the Hematoxylin blue. I, also, have never heard of using Gills in association with any Trichrome stain, but as a long time user and educator of using a one-step (Gomori's) trichrome stain (which is the Leica blue collagen stain), if you rinse with any water between the stain step and the acetic acid step, you will end up with a stain that is overpoweringly blue. Here are the steps copied from the Leica blue collagen kit instructions. Notice NO WATER RINSE between the stain and Acetic Acid Solution. It is a common mistake. 10. Gently mix the Gomori's Trichrome Blue Solution by swirling and allow to stand for 6 minutes. 11. Place sections in 1% Acetic Acid solution for 1 minute. I hope this helps. Sincerely, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Trichrome troubleshooting (Suzanne Martin) From: Suzanne Martin smar...@lcpath.com Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the last acid step, even lessening time and adding time in the Weigerts. Thoughts? Thank you. Suzanne HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RELIA Histology Careers Bulletin 7-02-2015 Have a Safe and Happy 4th of July Holiday!!
Hi Histonetters!!! I hope you Have a safe and Happy July 4th Weekend. I Have A Fun Idea to Share for Pool Parties And Cookouts. Sprinkle Red Pop Rocks And Blue Sprinkles On Iced Sugar Cookies To Make Edible Firecrackers!! I have some exciting opportunities that I want to pass along as well. Maybe they will look good to you or someone you know. RELIA'S Hot Histology Opportunities!! Supervisor of IHC - Long Island, NY A RELIA EXCLUSIVE! Lead Histotech - Great Falls, MT A RELIA Exclusive! Histotech - Bellevue, WA Histotechnician - Norfolk, VA $15K Sign-on bonus Histotechnician - Atlanta, GA Histology Tech - Asheville, NC IHC Tech - Waynesboro, VA Histotechnician - Nashville, TN Histology Equipment Repair - Tampa Bay, FL All of these jobs are full time permanent positions with some of the finest facilities in the country. My clients offer excellent compensation and benefits and most offer relocation assistance or sign- on bonuses. For more information for you or a friend please call me at the office at 866-607-3542 or on my cell at 407-353-5070 or shoot me at email at rel...@earthlink.net Have a Safe and Happy 4th of July!! Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Suggestions for staining ground substances in the Heart
Movat's pentachrome is unnecessarily complicated when you want to look only at one component of a tissue. Most ground substance (extracellular material between cells, collagen fibres etc) is weakly stainable with PAS, alcian blue pH2.5 or both. It's usual to stain with alcian blue first (Mowry's method). If you do the PAS first, the PAS-positive materials will also stain with alcian blue and be darker. For the reasons, see: Johannes, M.-L. and Klessen, C. (1984). Alcian blue/PAS or PAS/alcian blue? Remarks on a classical technique used in carbohydrate histochemistry. Histochemistry 80:129-132. Yamabayashi, S. (1987). Periodic acid-Schiff-alcian blue: a method for the differential staining of glycoproteins. Histochemical Journal 19:565-571. Reid, P.E. and Owen, D.A. (1988). Some comments on the mechanism of the periodic acid-Schiff-Alcian blue method. Histochemical Journal 20:651-654. If you can predict the PAS-AB effect without further reading, you already have a good understanding of how the traditional methods of carbohydrate histochemistry work! John Kiernan London, Canada = = = On 30/06/15, Wolfe, Christina christina.wo...@bms.com wrote: Hi all, We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Unsubscribe
Hello Lea, You must go to the website listed at the bottom of the email and unsubscribe. Here it is... http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best wishes, Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Alminde, Lea S [mailto:lea.almi...@tuhs.temple.edu] Sent: Wednesday, July 01, 2015 5:51 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Unsubscribe Lea S. Alminde Anatomic Pathology Supervisor Jeanes Hospital 215-728-2034 email lea.almi...@tuhs.temple.edu This electronic message is intended to be for the use of the named recipient, and may contain information that is confidential or privileged. This communication may contain protected health information (PHI) that is legally protected from inappropriate disclosure by the Privacy Standards of the Health Insurance Portability and Accountability Act (HIPAA) and relevant Pennsylvania Laws. You can direct questions concerning PHI or HIPAA to the Corporate Compliance and Privacy Officer at (215) 707-5605. If you are not the intended recipient, please note that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this message in error, you should notify the sender immediately by telephone or by return e-mail and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
