[Histonet] Alcian blue (was Suggestions for staining ground substances ...)
Dear Histonetters, I recently replied to a question about staining extracellular ground substance with alcian blue. Here is some more information. With the alcian blue-PAS sequence it is necessary to have a good alcian blue dye - the same as or functionally equivalent to the original alcian blue 8G. For the PAS-alcian blue sequence, other alcian blues may also be OK. Alcian blue from a batch certified (or rechecked) by the Biological Stain Commission (BSC) 5 or fewer years ago should be good for either procedure. Of the dyes commonly used to make up staining solutions for microscopy, alcian blue is the only only one that sometimes deteriorates, unpredictably and often quite quickly, as a dry powder in the bottle. Some alcian blue powders from the 1950s are still OK, but some very much younger ones are no good 5+ years after passing the BSC's analytical and staining tests. John Kiernan London, Canada http://biostain.com = = = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana LCS (Liquid Cover Slip)
Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Suggestions for staining ground substances in the Heart
Kristie, For heart you could try the elastic Trichrome, it will stain the ground substances and is faster than the Movat. If your ground substance is devoid of staining it could be the phosphotungstic acid. Is it 5%, and fresh? Are you using two changes at 5 min each? Continue with the 4micron sections, and use fresh solutions. If you can make the phosphotungstic yourself, do that. Commercial solutions sometimes do not store well. Hope this helps. Sincerely, Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP) Instructor/Education Coordinator Program in Histotechnology School of Health Professions UT M.D. Anderson Cancer Center 713.563-3481 -Original Message- From: Wolfe, Christina [mailto:christina.wo...@bms.com] Sent: Wednesday, 1 July 2015 1:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Suggestions for staining ground substances in the Heart Hi all, We are interested in staining ground substances in the heart. Are there other stains that will work beside the Pentachrome? We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain. In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining. We have tried sections cut at 4 and 6 microns. Any thoughts/suggestions? Kristie Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept. 812-307-2093 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotech position in Tampa, FL!!
Our state-of-the-art dermatologic surgery practice in New Tampa is seeking a motivated candidate with professionalism, attention to detail, and a strong work ethic to join our team! Beautiful work environment and great benefits. Responsibilities include Mohs specimen (frozen section) preparation as well as permanent section preparation. Experience with Mohs and/or immunohistochemistry isn't required, but is a plus! Monday through Friday position with hours worked falling between 8am and 6pm Please send resumes to ju...@seascapesurgerycenter.com Julie The Bowman Institute Seascape Surgery Center Phone: (813) 977-2040 Fax: (813) 977-3886 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Opportunity in Canada
Hi, I hope you guys are doing well. I am looking for a job opportunity in Canada and I will really appreciate it if you guys could help me. I am an Histotech. With both HT(ASCP)and HTL(ASCP)Certifications. I also have both QIHC(ASCP) and QLS(ASCP)Qualifications. Thanks, Isaac ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Distain Masson Trichrome
Could you please let me know what % of Ammonia water is used for distain Masson Trichrome? Or what % is most common used? Thank you. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana LCS (Liquid Cover Slip)
You certainly can air/oven dry your slides instead of running them down and coverslipping; we do that with the slides we take off in the morning when we get in. As to your problem with water/oil on your slides, something is not right in your dawn water rinsing, dehydration and/or clearing steps...but I'm sure I'm not telling you anything you don't know already. I would change out all the graded alcohols and xylenes so you know the right reagents are in your containers. Next, I would make sure that whomever is rinsing the slides, that they are using warm or even hot Dawn water and that they are rinsing long enough for your DAB slides. If you're also running APR you may want to do the air/oven drying to xylene method to maintain the chromogen binding. Other than these steps, I don't know what else to suggest. A long, long time ago when I worked in an old lab with water chilled pipes for air conditioning, I ran into a lot of humidity in the summer and actually had to add molecular sieve to my 100% alcohol in my dehydration set-up or my xylenes would get contaminated with water but we're talking the mid 70's so I'm hoping you're not running into something similar. Hope this helps, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Burnett, Brandy [mailto:bburn...@capecodhealth.org] Sent: Thursday, July 02, 2015 12:07 PM To: Sebree Linda A Subject: RE: Ventana LCS (Liquid Cover Slip) For IHC..We are rinsing them with dawn before running them down. Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Thursday, July 02, 2015 10:17 AM To: Burnett, Brandy Subject: RE: Ventana LCS (Liquid Cover Slip) Are you talking about IHC/ISH or Specials Brandy? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Burnett, Brandy [mailto:bburn...@capecodhealth.org] Sent: Thursday, July 02, 2015 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Distain Masson Trichrome
Usually the strong 28% is used but I always prefer using a diluted solution so I have more control on the distaining.René On Thursday, July 2, 2015 12:10 PM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: Could you please let me know what % of Ammonia water is used for distain Masson Trichrome? Or what % is most common used? Thank you. Lin S. Bustamante, B.S., H.T.(ASCP) VIBS Histology Laboratory Supervisor College Of Veterinary Medicine Texas AM University College Station, Texas 77843-4458 Phone: (979) 845-3177 Fax: (979) 458-3499 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cytologic and Frozen Specimens - IHC Techniques
Is anyone able to recommend a good reference on preparation techniques for immunostaining of cytologic and frozen specimens? I'm studying for the qIHC exam and I'm striking out with my usual sources (Carson, DAKO guide, etc.) on some of the more specific questions in these areas. Thanks! Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinical Research Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Bld 539, 224 Frederick, Maryland 21702 anna.cof...@nih.gov 301-846-1730 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Humidity
I have had an issue on a couple of occasions now. Our lab gets really humid and when I have a block that needs re-embedded, the tissue becomes saturated with water and difficult to cut. The tissue seemed properly processed before, but after embedding, it almost peels out of the block. Has anyone else had this problem and is so, do you have a solution. Thanks, Travis Troyer Peterson Laboratory Services Manhattan, KS ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
