Re: [Histonet] Tissue processing validation

[Tony Henwood (SCHN) via Histonet]

The validation would already have been done.

You only need to check if the re-location has affected any equipment components.

I would take a few cassettes of spare tissue and process it on your most used 
program and await the results.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: Ann Specian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, 22 July 2015 1:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue processing validation

We are moving out Peloris tissue processors to another room, so we have to 
validate.  I was wondering what other labs do as a validation for this type of 
move?  Do you run tissue on every progam utilizing each processor for at least 
one of the protocols?  Any help would be appreciated.
thanks,
Ann
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Re: [Histonet] Bone histology charges

[Shruti Shah via Histonet]

Hi All,

Can any one let me know general price for mouse bone decalcification, 
processing, microtome charges for per block or per slide.

it will great help.

Many thanks,


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[Histonet] PLA2R on paraffin sections- IHC versus indirect immunofluorescence

[Linda Margraf via Histonet]

Here is a message I am posting for Martha...


My renal pathologists want to add this antibody and I want to see what 
methodologies everyone is using.   The article I was reading mentioned both as 
an option.I would prefer to do IHC and run it on our Bond 3, as opposed to 
the very manual indirect IF method.   Any feedback would be appreciated.

Thanks in advance for your help!

Martha Ward, MT (ASCP) QIHC
Manager

Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC
mw...@wakehealth.edu

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[Histonet] Tissue processing validation (Ann Specian)

[Steve McClain via Histonet]

This seems like overkill. Just moving a processor previously validated should 
not require re-validation, just verify the programming and make certain the 
paraffins are melted completely. 
This may not apply to all processors, but for all Sakura machines manufactured 
since 1980,I would not revalidate.

Steve A. McClain, MD
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[Histonet] Decalcification and Neutralization (Huynh,Thomas)

[Steve McClain via Histonet]

I have not found the need for neutralization.
With sufficient washing, common histochemical stains including H&E Gram 
PAS-fungus and trichrome are spectacular.
Please explain your protocol for neutralization.

Steve A. McClain, MD

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[Histonet] email issues

[Linda Margraf via Histonet]

Dear Histonetters,
I have heard from a couple of list members that they have been removed from the 
list from excess bounces and once they have gotten back on the listserv 
membership list, they no longer receive a copy of any message they post to the 
list. They use Yahoo and AOL.

I contacted the IT experts at the university who investigated. It appears Yahoo 
and probably AOL have changed settings to improve spam filtering and it is 
blocking or bouncing mail. The IT guys changed some settings on the server. The 
bouncing issue seems to be resolved but for people using those email systems, 
they may not get a copy of any messages they post to the list any longer.  
Apparently all the other mail from the list comes through just fine. If that 
happens to you, you can check the archive 
(http://www.histosearch.com/histonet.html) to see if your message is there. 
There is also a setting for your Histonet subscription, that you can turn on, 
that will send an acknowledgement when you post to the list. I am testing this 
now as I don't think we have used it before. Yahoo and AOL (or other email 
providers) customer service may also be able to help.

To change or look at your personal listserv settings on the list visit 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Remember, if you get a message about being a non-member if you try to post a 
message, it means your email address has likely changed from when you first 
subscribed and it needs updating, which you can also do at this site.
Thanks,
Linda M
Histonet administrator
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[Histonet] Tissue processing validation

[Ann Specian via Histonet]

We are moving out Peloris tissue processors to another room, so we have to 
validate.  I was wondering what other labs do as a validation for this type of 
move?  Do you run tissue on every progam utilizing each processor for at least 
one of the protocols?  Any help would be appreciated.
thanks,
Ann
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