Re: [Histonet] Handling paraffin

[John Kiernan via Histonet]

Gloves and "Paraffin hazards?"

1. Flammable. White candles are made of paraffin wax. We light them ungloved. 
2. Hot when melted, but not hot enough to cause even a first degree burn from a 
small amount on the skin. 
3. Liquid enters clothing and solidifies therein. This is a real annoyance, but 
the safety experts couldn't care less. Wax is removable by soap & water at >60C 
(thank you, René Buesa) or by dry-cleaning. 
4. Solid wax has to be trimmed with a sharp knife to prepare for microtomy. 
Gloves thick enough to prevent a cut finger probably would impede this skilled 
task.
5. Every microtome has a dangerously sharp knife, less so in recent decades 
with short disposable blades. This is a hazard of microtomy, not of paraffin 
wax. It might be possible to cut, recover and mount sections while wearing very 
thin (surgical) rubber gloves, but why? 

John Kiernan
London, Canada
= = = 

On 03/08/15, "Johnson, Carole via Histonet"  
 wrote:
> This is kind of an odd question, but I was asked by a pathologist for any 
> SOPs or references for the necessity of wearing gloves when embedding and 
> working with paraffin. I am not aware of sources other than the MSDS for the 
> different formulations. Does anyone require gloves to be worn during 
> embedding, specifically related to paraffin hazards?
> 
> Carole Johnson
> Carole Johnson, HT(ASCP)cm
> New Mexico Department of Agriculture
> Veterinary Diagnostic Services
> 505.383.9299
> 
> To understand is to stand under, which is to look up, which is a good way to 
> understand
> 
> 
> 
> (http://lists.utsouthwestern.edu/mailman/listinfo/histonet)
> 
> 
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Re: [Histonet] Handline paraffin

[Esther C Peters via Histonet]

However, I would be concerned if using Paraplast Plus (+) because it contains 
dimethyl sulfoxide (DMSO) to infiltrate the tissue faster. It can be absorbed 
through bare skin and you get a garlic taste in your mouth, making you wonder 
what else was absorbed into your body (DMSO is used for transdermal drug 
administration). We wear gloves when working with molten Paraplast +, but not 
for microtomy of the solidified blocks.

Esther

Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epete...@gmu.edu
https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu



From: Rene J Buesa via Histonet 
Sent: Monday, August 3, 2015 12:52 PM
To: Johnson, Carole; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Handline paraffin

It is absolutely NOT necessary to wear gloves when working with paraffin. This 
is NOT a harmful or "irritating" substance. It is just an oil of high molecular 
weight (mineral oil)René


 On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" 
 wrote:


 This is kind of an odd question, but I was asked by a pathologist for any SOPs 
or references for the necessity of wearing gloves when embedding and working 
with paraffin. I am not aware of sources other than the MSDS for the different 
formulations. Does anyone require gloves to be worn during embedding, 
specifically related to paraffin hazards?

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

To understand is to stand under, which is to look up, which is a good way to 
understand




Confidentiality Notice: New Mexico has a very broad public records law. Most 
written communications to or from state employees are public records. Your 
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Re: [Histonet] Handline paraffin

[Karen Stephanson via Histonet]

Is there any written documentation?
On Aug 3, 2015 11:10 AM, Rene J Buesa via Histonet 
 wrote:
It is absolutely NOT necessary to wear gloves when working with paraffin. This 
is NOT a harmful or "irritating" substance. It is just an oil of high molecular 
weight (mineral oil)René


 On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" 
 wrote:


 This is kind of an odd question, but I was asked by a pathologist for any SOPs 
or references for the necessity of wearing gloves when embedding and working 
with paraffin. I am not aware of sources other than the MSDS for the different 
formulations. Does anyone require gloves to be worn during embedding, 
specifically related to paraffin hazards?

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

To understand is to stand under, which is to look up, which is a good way to 
understand




Confidentiality Notice: New Mexico has a very broad public records law. Most 
written communications to or from state employees are public records. Your 
e-mail communications may therefore be subject to public disclosure. This 
e-mail, including all attachments is for the sole use of the intended 
recipients. Any unauthorized review, use, disclosure or distribution is 
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Re: [Histonet] Average number of slides reviewed by a surgical pathologist

[Rene J Buesa via Histonet]

Please go to:
http://www.histosearch.com/rene.htmland download my article on "Staffing". The 
answer to your question is there.René 


 On Monday, August 3, 2015 5:20 PM, "Vickroy, James via Histonet" 
 wrote:
   

 I realize this is a question that may be impossible to answer given all of the 
variables in pathology labs, specimens, physicians, etc.  However here goes:  
For those that work in a lab that primarily does biopsies ( mainly gi biopsies) 
what is the average number of slides that a pathologist reads each day.    I 
have looked online for parameters recommended however I haven't had a whole lot 
of luck.  There are plenty of figures on numbers of blocks and slides for 
histotechs but just wondered if anyone was using any either national benchmarks 
or local practices.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
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taken on the contents of this information is strictly prohibited. If you have 
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Re: [Histonet] Histonet Digest, Vol 141, Issue 1

[Subash Govender via Histonet]

 Original Message 
Subject: Histonet Digest, Vol 141, Issue 1
From: histonet-requ...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
CC:

Send Histonet mailing list submissions to
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Today's Topics:

   1. Re: Ventana H.Pylori antibody (White, Marcia)
   2. (no subject) (Teresa Harris)
   3. P16 (kalkhenaizi .)
   4. Re: Breast fixation (Cartun, Richard)
   5. Re: Ventana H.Pylori antibody (Cates, Julia)


--

Message: 1
Date: Fri, 31 Jul 2015 17:09:00 +
From: "White, Marcia" 
To: "'Jeffrey Robinson'" , "Cates,
Julia"  , "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Ventana H.Pylori antibody
Message-ID:
<8b4b5bf3fcb6d24cbeaa63aca5fdad3b51490...@mhsexmb04.mhs.net>
Content-Type: text/plain; charset="us-ascii"

We also are having the same problem with the Ventana H.Pylori antibody on our 
Ultras, I have tried the CellMarque product in the past and as mentioned still 
not ideal. Will try the Biocare product and see if the results improve for us 
also.


Marcia M White
Pathology Manager
Memorial Regional Hospital
954-265-5371-office
954-967-7627-fax
mwh...@mhs.net







-Original Message-
From: Jeffrey Robinson [mailto:jrobin...@pathology-associates.com]
Sent: Thursday, July 30, 2015 1:06 PM
To: Cates, Julia; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana H.Pylori antibody

Hi Julia-  I have had a lot of experience trying to get a clean H. pylori.  I 
don't recall using the Ventana H. pylori but I was using the CellMarque H. 
pylori for years.  It would often have a lot of background as you describe.  I 
run Ventana Benchmarks (XT and Ultra) and a Leica Bond.  The slides I would run 
on the Benchmarks were usually cleaner than those run on the Bond but still not 
as clean as I would like. I finally switched over to BioCare's H. pylori after 
many complaints from my pathologists.  It has been much cleaner on all of my 
IHC instruments.  Actually, the ones I run on the Ultra are the cleanest I have 
ever seen.  The slides run on the Bond may still have a little background but 
it is still much cleaner than the ones I used to run with the CellMarque 
antibody.  One of my pathologists who used to complain about the excessive 
background now calls it his "go to" stain and is very happy with how crisp it 
is. The organisms stand out very nicely.  BioCare will send you a 
 free sample if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-Original Message-
From: Cates, Julia via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody

Good day Histonet,

I have seen in the past conversations regarding Ventana's H.Pylori antibody and 
how dirty looking the stain can be.  We have been using this antibody for 
several years now and have never really been happy with the quality.  We have 
tried the recommended methods to "clean up" the stain and still we run into 
repeating the stain and/or complaints from the pathologists.  We are 
considering using a different vendor but my concern is that my efforts will be 
a lateral move.  Is anyone using a product that produces a clean stain or is 
this something inherent to this antibody?  Are the pathologists happy with it 
and not just tolerating it?

Thank you for your suggestions,

Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253- ext.4346 | Fax: (352) 253-3592

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[Histonet] Average number of slides reviewed by a surgical pathologist

[Vickroy, James via Histonet]

I realize this is a question that may be impossible to answer given all of the 
variables in pathology labs, specimens, physicians, etc.  However here goes:  
For those that work in a lab that primarily does biopsies ( mainly gi biopsies) 
what is the average number of slides that a pathologist reads each day.I 
have looked online for parameters recommended however I haven't had a whole lot 
of luck.  There are plenty of figures on numbers of blocks and slides for 
histotechs but just wondered if anyone was using any either national benchmarks 
or local practices.

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
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taken on the contents of this information is strictly prohibited. If you have 
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immediately, by electronic mail, so that arrangements may be made for the 
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[Histonet] Glutamine Synthetase

[Sebree Linda A via Histonet]

Good afternoon,

Our lab is looking for a reference lab to send FFPE  slides for this antibody.  
The last place we sent them to was unsatisfactory.

Thank you in advance,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174

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Re: [Histonet] Handline paraffin

[Rene J Buesa via Histonet]

It is absolutely NOT necessary to wear gloves when working with paraffin. This 
is NOT a harmful or "irritating" substance. It is just an oil of high molecular 
weight (mineral oil)René 


 On Monday, August 3, 2015 12:48 PM, "Johnson, Carole via Histonet" 
 wrote:
   

 This is kind of an odd question, but I was asked by a pathologist for any SOPs 
or references for the necessity of wearing gloves when embedding and working 
with paraffin. I am not aware of sources other than the MSDS for the different 
formulations. Does anyone require gloves to be worn during embedding, 
specifically related to paraffin hazards?

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

To understand is to stand under, which is to look up, which is a good way to 
understand




Confidentiality Notice: New Mexico has a very broad public records law. Most 
written communications to or from state employees are public records. Your 
e-mail communications may therefore be subject to public disclosure. This 
e-mail, including all attachments is for the sole use of the intended 
recipients. Any unauthorized review, use, disclosure or distribution is 
prohibited unless specifically provided under the New Mexico Inspection of 
Public Records Act.
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[Histonet] Handline paraffin

[Johnson, Carole via Histonet]

This is kind of an odd question, but I was asked by a pathologist for any SOPs 
or references for the necessity of wearing gloves when embedding and working 
with paraffin. I am not aware of sources other than the MSDS for the different 
formulations. Does anyone require gloves to be worn during embedding, 
specifically related to paraffin hazards?

Carole Johnson
Carole Johnson, HT(ASCP)cm
New Mexico Department of Agriculture
Veterinary Diagnostic Services
505.383.9299

To understand is to stand under, which is to look up, which is a good way to 
understand




Confidentiality Notice: New Mexico has a very broad public records law. Most 
written communications to or from state employees are public records. Your 
e-mail communications may therefore be subject to public disclosure. This 
e-mail, including all attachments is for the sole use of the intended 
recipients. Any unauthorized review, use, disclosure or distribution is 
prohibited unless specifically provided under the New Mexico Inspection of 
Public Records Act.
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[Histonet] RELIA Histology Careers Bulletin Special Edition for Managers and Supervisors - 8-3-2015

[Pam Barker via Histonet]

Hi Histonetters!!
What would make the perfect management position?
Is it the location?
Perhaps the type of lab?
How about the size of the staff/lab?
Maybe it's the Hours? The Money and/or benefits?
Could it be that a particular situation is the next logical step in your
career?

More than likely the answer is all of the above in different degrees.
That is why I am contacting you with this special bulletin.  
I am getting more and more management opportunities every day and 
I wanted to touch base and let you know.  
Your next opportunity might be just around the corner and it could be right
here with me!!  
If you are looking for a position right now please contact me right away.
We can talk about my current positions OR about a customized search on your
behalf. 
 If you aren't looking right away but want to let me know what would make a
perfect job for you so that I could keep an eye out,  shoot me an email at
rel...@earthlink.net or call me toll free at 866-607-3542 and let me know!!


Here is a list of my current managerial opportunities:
Anatomic Pathology Manager - Hammond, IN
Histology Supervisor Macon, GA
Histology Supervisor - Dallas, TX
Histology Supervisor - Flagstaff, AZ
IHC Supervisor - Long Island, NY
IHC Supervisor - Waynesboro, VA
Lead Histotech/Supervisor - Great Falls, MT
Lead Histotech/Supervisor - Waco, TX
Lead Histotech - Atlanta, GA

All of these clients offer autonomy in your position and histology staff
eager to welcome their new manager.

I really appreciate you taking time out of your busy day to read my e-mail 
 

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia


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[Histonet] Cerner Computer System

[Davis, Cassie via Histonet]

Good Morning Histonet,

 I am hoping to touch base with anybody using Cerner. We have just 
been told we are switching and are wondering if it works with CoPath and 
Vantage. Any input would be appreciated.

Cassandra Davis
Histology Technician
Anatomical Pathology Laboratory
Saint Francis Healthcare
701 N. Clayton Street
Wilmington,DE 19805
Office:  302-575-8095
Email:  cda...@che-east.org
www.saintfrancishealthcare.org


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Re: [Histonet] Breast fixation

[Victoria Baker via Histonet]

Karen
We take ours off on Sunday.  The tissue processor is set up with 6 hours of
fixation the minimum time.  We have VIP 5's so on Friday the tissue goes on
a delay run that is set to come out of formalin at 12:30 AM Sunday
morning.  The end of the process is at 9 AM Sunday morning.  Just take the
tissue off and let it solidify.   When your next tech comes in they can put
the cassette in the embedding center to heat up again and embed it.  You
will follow your same protocol and record your times accordingly.  It might
mean having to ask someone to come in for the 5 minutes it takes to do this
but it works.  If needed you can adjust your times to fit your schedule for
someone being available to take them off.
I hope this will help.
Vikki
On Jul 29, 2015 11:33 AM, "Heckford, Karen - SMMC-SF via Histonet" <
histonet@lists.utsouthwestern.edu> wrote:

> Good Morning,
> I have a question about breast fixation.   I am in a little bit of a
> pickle with the 6-72 hour rule for the fixation on breast tissue.   Friday
> I am getting 2 breast cases in the afternoon and both will not have the
> required minimum 6 hour formalin fixation for my per diem to cut early
> Saturday morning.   He will not be able to make it in again until Monday
> night.  The tissue will be about 3-4 hours (this includes time on the
> processor)  over the 72 hour maximum.Does anyone have any suggestions
> on what can be done?  We are a one person show here.
>
> Thanks,
>
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckf...@dignityhealth.org
>
> Caution:  This email message, including all content and
> attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY
> PRIVILEGED.  The information contained in this email message is intended
> only for the use of the recipient(s) named above. If the reader of this
> message is not the intended recipient or an agent responsible for
> delivering it to the intended recipient, you have received this document in
> error.  Any further review, dissemination, distribution, or copying of this
> message is strictly prohibited.  If you have received this communication in
> error, please notify us  immediately by reply email.  Thank you."
>
>
>
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[Histonet] Position Maryland

[Michael LaFriniere via Histonet]

Our laboratory has Full Time Supervisory HT (ASCP)  job position opening for 
right candidate in Silver Spring Maryland, large independent AP/lab with 
hospitals, surgery centers and physician office clientele. Further information 
please  contact me directly.


**Not interested in job placement companies, head hunters or 
placement service  calls or contacts ***

Michael
Michael R. LaFriniere, HT (ASCP)
Executive Director


Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904  
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844
michael.lafrini...@ccplab.com






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