Re: [Histonet] Tissue request....

[SALLY A DREW via Histonet]

Just a suggestion...Is this need for materials for review for formalin-fixed, 
paraffin-embedded tissues? Is this for human tissue? These are just two 
questions that need to be answered in order before any willing source can speak 
up.



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 Original message 
From: Rachel M Gonzalez via Histonet 
Date: 09/18/2015 6:20 PM (GMT-06:00)
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Tissue request

Hi

Looking for control tissue for reviewing antibody specificity this is for
R project. I do not know if that will make a difference in the tissue
availability but I thought I put that out there.  If you know a good tissue
source can you let me know.

I need three of each type

normal bone marrow (hematopoietic cell and lymphocytes, and etc.),

normal brain/cerebrum,

normal cerebellum,

normal parathyroid glands,

normal hypophysis/Pituitary Gland

normal thymus.



Thank you in advance for help.

Rachel Gonzalez PhD

Senior Scientist
.
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[Histonet] Retics on Ventana Benchmark Special Stainer

[Jeffrey Robinson via Histonet]

Good Morning to all Histotechs-  There have been many questions over the past 
several months regarding poor retic staining on the Ventana Benchmark Special 
Stainer.  With the help of my histotech friends, I have now achieved successful 
staining on both liver and bone marrow specimens utilizing this instrument.
My primary source of protocol information has been Heather Nowacki at the Mayo 
Clinic.  Heather says that they use B-5 fixative and RDO Gold decalcifier on 
their bone marrow cores.  We use B Plus fixative and Immunocal  decalcifier so 
the choice of an appropriate fixative and decalcifier does not seem to be a 
concern in the ability of this instrument to achieve the desired retic staining 
intensity.

Here is Heather's protocol for retic:  Heat:  45 degrees C.
 
Oxidizer:  4 minutes
 
Decolorizer: 4 minutes
 
Sensitizer:  8 minutes
 
Silver:  12 minutes
 
Nuclear Fast Red Counterstain:  4 minutes

I did not need to modify any of Heather's protocol options.  I had previously 
been unable to achieve any satisfactory staining on bone marrow retic staining 
on the Benchmark Special Stainer.  I showed my bone marrow retic test slides to 
one of my pathologists and he rated them comparable to the retic slides we are 
currently staining on the old Ventana Nexes stainer.

Notes:  It seems that the primary issue affecting proper retic staining on the 
Benchmark Special Stainer has been the lack of sufficient heat applied to the 
slides during the staining run.  Ventana has released a software upgrade that 
allows for a much wider range of heating temperature options during retic 
staining.  You will need to have the new software upgrade installed by Ventana 
before you will be able to use this protocol.  Ventana also discussed an 
enhanced decontamination protocol for the Benchmark Special Stainer that 
requires some additional reagents but the Ventana technical rep who installed 
my upgrade determined that the enhanced decontamination protocol was not 
necessary in our lab.  She felt that this enhanced protocol would only be 
beneficial in labs that have had major contamination issues (including Special 
Stains Wash pH changes) in the past.  We cut all of our retic slides at 5 (even 
for the Nexes) and I have been quite happy with the staining intensity on slides
  cut at that thickness.  Slides cut at 3 or 4 stain too light for my taste.

Acknowledgements:  I would like to thank Tim Morken for spotting the Mayo 
Clinic poster on Ventana Benchmark Special Stainer Retic staining at NSH last 
month.  I would also like to thank the team at the Mayo Clinic who worked on 
this project allowing the rest of us in the field to benefit from their hard 
work.  The Mayo team includes:  Heather Nowacki, HTL;  Mark Keith, HTL;  Scott 
Antilla, HTL;  and Joaquin Garcia, M.D.

I hope this information is of use to my fellow histotechs who have the Ventana 
Benchmark Special Stainer in their labs.

Jeff Robinson, HT, HTL, Senior Histotechnologist, Sierra Pathology Lab, Clovis, 
CA.


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[Histonet] H. pylori control tissue

[Cartun, Richard via Histonet]

Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori.  I 
have tried e-mailing her, but my e-mail was returned as "Undeliverable".  
Nancy, please contact me.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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[Histonet] Tissue request....

[Rachel M Gonzalez via Histonet]

Hi

Looking for control tissue for reviewing antibody specificity this is for
R project. I do not know if that will make a difference in the tissue
availability but I thought I put that out there.  If you know a good tissue
source can you let me know.

I need three of each type

normal bone marrow (hematopoietic cell and lymphocytes, and etc.),

normal brain/cerebrum,

normal cerebellum,

normal parathyroid glands,

normal hypophysis/Pituitary Gland

normal thymus.



Thank you in advance for help.

Rachel Gonzalez PhD

Senior Scientist
.
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[Histonet] Validation

[Erskine Husbands via Histonet]

Does anyone in Histoland have any information on validation of H.pylori 
immunohistochemical staining,
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[Histonet] CD34

[Bernice Frederick via Histonet]

Been looking around a bit and seem to have it a roadblock. Does anyone know of 
a vendor for CD34 that will cross react with rabbit? On frozen sections, mind 
you.
Thanks,
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

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[Histonet] H. Pylori

[Nancy Schmitt via Histonet]

Good Morning-

I am looking for H. Pylori control - does anyone have any extra lying about?  
What are you currently using?  Purchasing?
I appreciate any and all feedback!

Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA  52001
Check us out at www.uclaccess.com





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[Histonet] IHC specialist

[Richardson, Pam K via Histonet]

Would anyone be able to provide a job description for an IHC specialists?

Cordially,

Pam ~
+++
Pam Richardson
Clinical Manager
Gundersen Health System Laboratory Services
Email: pkric...@gundersenhealth.org
Phone: 608 775-4133
Fax: 608 775-6136
Interdepartmental Mail Stop: H04-007
E-visit us at: http://www.gundersenhealth.org

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[Histonet] Tissue Processors Reviews

[ODea, Elise via Histonet]

I am looking for experiences and opinions on Sakura VIP6 and Excelsior 
processors?

Thank you,
Elise
Elise T. O'Dea, MT, ASCP
Histology Supervisor
Highland Hospital
1000 South Ave.
Rochester, New York 14620
office 585.341.6596
lab 585.341.8314
elise_o...@urmc.rochester.edu


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[Histonet] Source Codes

[Arrington, Karla A via Histonet]

To All:

Our hospital is going over their specimen source codes and updating them. I am 
asking for suggestions in finding out if there is an already
list out there that includes all the specimen sources and CPT codes.  Also, if 
one gets a multiple organs specimen received in one container, can
one code the organs individually, or is just simply one code because it came in 
one container?

Thanks,

Karla Arrington, HT(ASCP) HIT(AHIMA)
Pathology Supervisor
ANMC
4315 Diplomacy Drive
Anchorage, AK 99508
Phone: 907-729-1810
Fax: 907-729-1226
kaarring...@anthc.org

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