[Histonet] P16 alternative

[Charles Riley via Histonet]

Does anyone know an alternative to Ventana's P16 antibody for testing for
viral inclusions like HPV using IHC?

-- 

Charles Riley HT(ASCP)CM
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[Histonet] ALK-1

[raestask--- via Histonet]

Does anyone out there have a protocol for ALK-1, using the Benchmark Ultra and 
Ventana Ultraview detection kit? If so I would greatly appreciate it. Thanks in 
advance. 

Rae Staskiewicz 
Unitypoint Health-Methodist Peoria 

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[Histonet] Job opening in Abilene TX

[Armstrong, Karoleigh T via Histonet]

Hello Histonet,

There is a job opening for a Histologist in Abilene Tx. We are a 231 bed 
hospitial in Central West Texas. We have 3 Universities and 2 colleges, a 
livley arts and culteral life. Home of the 10 minute rush hour!

Please contact our website for more info. at, wwwabileneregional.com

KK Armstrong H.T.(ASCP) Sup. of Histology


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[Histonet] DIF on paraffin embedded tissue

[Morken, Timothy via Histonet]

The response to a problem like this is that your lab has not validated such a 
procedure for diagnostic use so the results are completely unreliable with your 
current protocols. Sure, you can "try it" but a negative result would mean 
nothing at all. Or you might get massive background that also means nothing. To 
validate it would take days to weeks depending the number of stains, not to 
mention getting some the reagents necessary to get a good signal, and the many, 
many hours you would spend working it up. The ideal solution would be to find 
another lab that does have this validated and let them do it.

Your lab could validate it and have it ready for the next time, but it would 
probably be a rare procedure and many of the reagents would expire between uses.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143

(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.mor...@ucsfmedctr.org



Maryann Deathridge madeathridge at pastnashville.com 

Wed Nov 25 09:37:36 CST 2015


We have a tissue sample that was processed and paraffin embedded.  We

URGENTLY need to recover the tissue and perform Immunofluorescence on the

sample.

 Does anyone have a procedure.   HELP



 madeathridge at 
pastnashville.com


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[Histonet] Histotech II-Athens Regional Medical Center, Athens, GA

[Pam DeFazio via Histonet]

*Job Description: *

Summary:
Accurately registers and accessions specimens into the information system
and resolves specimen integrity and identification issues. Responsible for
the proper control and processing of tissue and fluid specimens. Performs
routine and specialized histology/cytology techniques and staining;
troubleshoots and resolves stain performance. Performs all routine and
unscheduled maintenance and operation of automated Histology equipment,
facilitates repair, and uninterrupted section operation; Completes clerical
tasks in the Histology section.



*Skills or Responsibilities required by position:  *

Responsibilities:
Able to embed and perform microtomy on all tissue specimen types. Able to
properly process all fluid types for cytologic examination. Operates
biomedical instrumentation and equipment, troubleshoots, and resolves
problems. Organizes workload and information efficiently. Strong verbal,
written, and interpersonal skills. Able to perform day to day section
responsibilities and tasks independently and resolve problems. Works
successfully as part of a team to provide section coverage and to manage a
dynamic workload. Efficiently uses information systems.



*License or Credentials required by position:*



 Education: High School Diploma
Hours:  3:30pm- 12:00


www.athenshealth.org
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[Histonet] CAP and Special Stains Question

[Morken, Timothy via Histonet]

Diana,

Obviously there are no negative controls for some special stains because they 
just stain tissue elements (ie, trichrome) that are often in all tissues and we 
are just looking at the morphology changes in a disease state. A negative would 
have you apply the stain to tissue that could not be stained by the stain, or 
not apply the specific stain to show there is no interference from other 
reagents used. The question is whether that is useful for tinctorial stains.

The solution to these is to put some verbiage in your procedure that this does 
not require a negative control and why. You should have clear description, and 
pictures of what an acceptable stain looks like, and even more important, what 
a failed stain looks like.

However, a negative should be used for organism stains, and could be useful for 
some specific tissue elements. For example,  congo red or iron, you can leave 
off the specific stain and just use the counter stain (it could be useful - if 
the control is cut thru for instance the positive and negative would look the 
same).

I know well that this is not the normal practice going back many decades, and 
we argued with the Joint Commission about this as well, but regulatory agencies 
are becoming stricter about validations and controls, so it could be a good 
thing to prove your systems work as intended and to catch the rare failure.

Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


Good day,



I have a question that hopefully I can get help:







We are preparing for our CAP Inspection for 2016. The ANP: 21395 requirements 
states:







For special stains, including histochemical stains, and studies using 
immunologic and FISH/ISH methodology, positive and negative controls are 
verified and recorded as acceptable prior to or concurrent with the reporting 
of patient results and records maintained.







Evidence of Compliance:



Records for verification of control acceptability (prior to completion of 
associated cases)



What I understand from this requirement is not only do they want a positive 
control compliance but also a negative control compliance for special stains, 
so if we need a negative controls for special stains how do you go around doing 
a negative control? Do we use a tissue not specific for the special stain such 
as an Congo Red using a tissue without amyloid or eliminate a reagent from the 
protocol? Please help! Thanks!







Diana Martinez-Longoria



Bachelors of Science in Biology



 Histotechnician (ASCP)cm



El Centro Regional Medical Center



Phone: 760-339-7267



Fax: 760-339-4570



Email:dmlongoria at 
ecrmc.org



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