Re: [Histonet] embalmed cadaver tissue

[Bob Richmond via Histonet]

Nancy Schmitt at pa-ucl.com asks: >>Does anyone work with embalmed cadaver
tissue?  How is this handled for successful processing?<<

Funeral directors embalm bodies using a solution of formaldehyde, alcohol,
and dyes. I've autopsied a number of embalmed bodies. Tissue is sort of
half-fixed, and will usually finish fixing quite satisfactorily if the
pathologist puts pieces of tissue of the customary size into neutral
buffered formalin overnight.

If an embalmed body is to be autopsied, only the arterial embalming should
be done first. If they do the "cavity embalming" as well they make a mess
of the organs. This is something that needs to be worked out with the
embalmer in advance.

You can't do microbiological cultures on an embalmed body - that's the
major disadvantage.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] contents of Histonet Digest, Vol 145, Issue 10

[Hans B Snyder via Histonet]

Message: 3
Date: Wed, 9 Dec 2015 19:49:37 +
From: "Vickroy, Timothy" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] Use of microarrays or tissue rolls for immuno
controls
Message-ID: <32332277dfb64786a26649d55d2ec9f5@EXCHSRVR15.mshmc.local>
Content-Type: text/plain; charset="us-ascii"

We are looking at the possible use of microarrays or tissue rolls for
immuno controls on a routine basis. I would like to talk to people who are
currently doing their immunos this way.
We run about 120 slides a day with about 170 different primaries. Anyone
with previous experience in this area (good or bad) who has any
suggestions, I would be glad to hear about them.
 Thank you,
Tim Vickroy
Hershey Medical Center
Penn State University


Hello Tim,

We do not use TMA's but are affiliated with a company (
http://jmdhistology.com/) that makes them.  They have fast turnaround times
of 2 weeks at $5.00 per core.  They use a new proprietary system that
allows us to make them very fast and can have multiple different sizes in
the same block.

Just in case you need them.

Thank you

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com 

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On Thu, Dec 10, 2015 at 1:00 PM, 
wrote:

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> Today's Topics:
>
>1. Cork-covered beakers (Morken, Timothy)
>2. embalmed cadaver tissue (Nancy Schmitt)
>3. Use of microarrays or tissue rolls for immuno controls
>   (Vickroy, Timothy)
>4. Blog post--not work related (Lester Raff MD)
>5. A bad link--again (Lester Raff MD)
>
>
> --
>
> Message: 1
> Date: Wed, 9 Dec 2015 18:29:26 +
> From: "Morken, Timothy" 
> To: Histonet 
> Subject: [Histonet] Cork-covered beakers
> Message-ID:
> <761e2b5697f795489c8710bcc72141ff60335...@ex07.net.ucsf.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all, we have some old beakers that we use for hot solutions that have
> cork covering over the vertical portion of the beaker The cork insulates
> for easy handling. I have not been able to find replacements for these.
> Does anyone have a source for these beakers? Or a method to cover them with
> the cork?
>
> Thanks!
>
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
>
> --
>
> Message: 2
> Date: Wed, 9 Dec 2015 18:34:07 +
> From: Nancy Schmitt 
> To: "'histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] embalmed cadaver tissue
> Message-ID: <6d1a978a1371471b80d67453f278d...@mercury.wad.pa-ucl.com>
> Content-Type: text/plain; charset="us-ascii"
>
> Good Afternoon!
> Does anyone work with embalmed cadaver tissue?  How is this handled for
> successful processing?
> Any advice appreciated.
> Nancy
>
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> --
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> Message: 3
> Date: Wed, 9 Dec 2015 19:49:37 +
> From: "Vickroy, Timothy" 
> To: 

[Histonet] curling of kidney sections in water path

[mohamed abd el razik via Histonet]

dear all, i'm facing an issue that trouble us when we process tissue samples of 
different organs and cutting them; all organs are ok and spread fine and 
somoothly on ater path except for kidney sections (looks wrinkled and curled as 
if the praffin fram around it prevent it from spread!!!). i have tried using 
alcohol befor path and elevating the water temprature but no change in the 
sctions happened even after stay in the incubator) any suggestions please 

thank you for your help
Mohamed 
Cairo University
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[Histonet] A bad link--again!!!!

[Lester Raff MD via Histonet]

I apologisze-gotta do something about this site!

Here is the correct link!

http://www.chicagonow.com/downsize-maybe/2015/12/what-i-learned-on-the-it-hurts-too-much-to-eat-diet/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Blog post--not work related

[Lester Raff MD via Histonet]

Just in time for the holidays!


http://www.chicagonow.com/downsize-maybe/2015/12/what-i-learned-on-the-it-hurts-too-much-to-eat-diet/


Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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