Re: [Histonet] Price gun

[Dessoye, Michael via Histonet]

Check this out: 
http://www.staples.com/Garvey-reg-1-Line-Pricemarker/product_69516

Lots of types available at Staples, Grainger, etc.  It will save you a lot of 
writing.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre 
General Hospital | An Affiliate of Commonwealth Health | 
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 
| Tel: 570-552-1432 | Fax: 570-552-1486


-Original Message-
From: Histology Technician [mailto:histology81...@att.net] 
Sent: Friday, January 08, 2016 9:43 AM
To: Histonet List; histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] Price gun

  I was thinking of using a price gun to date my supplies when they come in, 
but I can't find one to buy.  Any ideas where to search for one?What do you 
guys use to date your supplies?  I've always used a pen to write the rcd date 
and my initials but thought a price gun would be quicker and neater :) Thanks!


--
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[Histonet] Price Gun

[Brent Adams via Histonet]

I ordered mine on ULINE.com and got it for $128.00 they also supply
the sticker rolls. This helped me as I get standing orders once a month.
I apply the received sticker next to the containers exp: date. When I open
the container and only use a portion. I circle the EXP: Date, and Received date 
sticker
in Red and hand write the date it was opened.

This has expedited being able to put supplies away and looks a lot cleaner
during inspections.

good luck

Brent Adams – BS, LPN, HT


www.acadianagastro.com

Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA  70503

tel:  (337) 269-1126
fax:  (337) 269-1476


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Friday, January 8, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 146, Issue 6

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Today's Topics:

   1. NSH Financial and Tax Statements (WILLIAM DESALVO)
   2. H and E Staining in IHC (Steven Weston)
   3. Re: NSH Financial and Tax Statements (Walter Benton)
   4. Re: IHC with H & E staining (Amos Brooks)
   5. Re: NSH Financial and Tax Statements (Chad L McMahan)
   6. Re: Histonet Digest, Vol 146, Issue 4 validation suggestions
  for short cycle processing with conventional equipment (Steve McClain)
   7. lab humidifiers (Sanders, Jeanine (CDC/OID/NCEZID))
   8. Thanks for assistance (Lester Raff MD)
   9. Price gun (Histology Technician)
  10. Re: lab humidifiers (Michael Ann Jones)
  11. HPV 16/18 background (Edison Narvaez)
  12. Happy New Year!  Here's to a Fantastic 2016! (Pam Barker)
  13. Re: lab humidifiers (Sanders, Jeanine (CDC/OID/NCEZID))


--

Message: 1
Date: Thu, 7 Jan 2016 14:31:44 -0700
From: WILLIAM DESALVO 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] NSH Financial and Tax Statements
Message-ID: 
Content-Type: text/plain; charset="Windows-1252"

As a due paying member of NSH, I ask all members and anyone interested in 
Histotechnology request from NSH office to see the Financial and Tax Statements 
for 2013 / 2014. This is public information. We should all know where our dues 
money, money from educational activities and vendor money from the convention 
is being spent.

Sent from my Windows Phone

--

Message: 2
Date: Thu, 7 Jan 2016 22:46:59 +
From: Steven Weston 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] H and E Staining in IHC
Message-ID: 
Content-Type: text/plain; charset="us-ascii"

Hi Marcus,
As stated previously by others there should be no problem putting the Eosin 
into your sections but I would suggest that you do not leave out the endogenous 
quenching so you can show your red blood cells. The reason being that there are 
many other cells that may then show up as positive. Instead I suggest using an 
Eosin/Phloxine mixture which nicely highlights the red cells differently to the 
rest of the general matrix material.
regards
Steve Weston
Breathe-Well CRE
Lab Manager
0408990859




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the intended recipient organisation is prohibited and may be a criminal 
offence. Please delete if obtained in error and email confirmation to the 
sender. The views expressed in this email are not necessarily the views of the 
University of Tasmania, unless clearly intended otherwise.


--

Message: 3
Date: Thu, 7 Jan 2016 22:59:00 +
From: Walter Benton 
To: WILLIAM DESALVO , Histonet

Subject: Re: [Histonet] NSH Financial and Tax Statements
Message-ID: <1452207540684.14...@cua.md>
Content-Type: text/plain; charset="iso-8859-1"

The website has some of the information you are speaking about, but I don't 
think tax statements are usually provided. They have the financials there for 
your review and I have included the link as well.

Re: [Histonet] Pricing Gun

[Dunn, Terry via Histonet]

Staples and Office Depot carry them.   They seem to run $100-200.

Terry Dunn
Sr. Manager, Workflow Consulting
Roche Diagnostics
Direct (520) 730-9596
terry.d...@roche.com
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Re: [Histonet] better than a price gun

[Terri Braud via Histonet]

UAL (United Ad Label) has a label guns, similar to the old paper labeler which 
puts wonderful labels, your choice of rec'vd date, date open, date exp...or any 
combination of the 3. 

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
   9. Price gun (Histology Technician)

Message: 9
Date: Fri, 8 Jan 2016 14:42:33 + (UTC)

? I was thinking of using a price gun to date my supplies when they come in, 
but I can't find one to buy.? Any ideas where to search for one?What do you 
guys use to date your supplies?? I've always used a pen to write the rcd date 
and my initials but thought a price gun would be quicker and neater :) Thanks!

--

Message: 10
Date: Fri, 8 Jan 2016 15:01:08 +
From: Michael Ann Jones 
To: "Sanders, Jeanine (CDC/OID/NCEZID)" ,
"histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] lab humidifiers
Message-ID: 
Content-Type: text/plain; charset="iso-8859-1"

We are struggling with our humidity constantly.
Colorado is dry in the winter.
We use at least two humidifiers to try to keep humidity at minimum 30% due to 
equipment manufacturer?s specs.
We have log sheets and thermometer/humidifier measurers to keep track of 
humidity.
Standard for equipment seems to be 30 - 80% humidity per manufacturer?s 
specifications of processors, etc.
Good luck 


Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

Providing collaborative diagnostic services, saving lives today and tomorrow.






On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet"
 wrote:

>Morning everyone!
>
>What is the standard in humidity levels that most histology labs strive 
>for? And to achieve this goal, do any of you use a commercial-grade 
>humidifier?
>
>Thanks for your help and have a nice weekend!
>
>
>Jeanine H. Sanders
>Centers for Disease Control and Prevention
>1600 Clifton Rd., NE MS-G32
>Atlanta, GA 30329
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet




--

Message: 11
Date: Fri, 8 Jan 2016 15:35:17 +
From: Edison Narvaez 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] HPV 16/18 background
Message-ID: <10d75e4b1c4f450ca1a4686118969...@ramexchange.hps.LOCAL>
Content-Type: text/plain; charset="us-ascii"

Hello,
I'm running HPV probes from Enzo on my Leica instruments, and we are noticing 
background episomal pattern on 16/18 staining of cases that are strongly 
positive for HPV 6/11, but not on cases that are 6/11 or 16/18 negative.
Is anybody experiencing this same issue and are there any suggestions.

I'm using Enzo probes, Enzo Stringency wash, Enzo mouse-antibiotin, and a 
polymer detection kit.

Thank you










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confidential and/or privileged information. Further, any medical information 
herein is confidential and protected by law. It is unlawful for unauthorized 
persons to use, review, copy, disclose, or disseminate confidential medical 
information. If you are not the intended recipient, immediately advise the 
sender and delete this message and any attachments. Any distribution, or 
copying of this message, or any attachment, is prohibited.


--

Message: 12
Date: Fri, 8 Jan 2016 10:31:34 -0500
From: "Pam Barker" 
To: "Histonet" 
Subject: [Histonet] Happy New Year!  Here's to a Fantastic 2016!
Message-ID: <00b601d14a29$a87eeca0$f97cc5e0$@earthlink.net>
Content-Type: text/plain;   charset="us-ascii"

Hi Histonetters!
Happy New Year
Here's to a terrific 2016!!!
How did you ring in the New Year? 
I hope you had fun ushering out 2015 and welcoming in 2016.  
I know I am looking forward to an exciting new year.
 
I wanted to drop you this quick line to let you know that I have been chatting 
with clients and have some exciting new opportunities.  
Here is a list of my current open positions:
Leadership:
Histology Lab Manager - Modesto, CA
Histology Supervisor (Dermpath) - Fayetteville, AR Senior Histotech - Norfolk, 
VA  B.S. required

Histotechnicians/Histotechnologists:
Histology Tech - Norfolk, VA 15K Sign on Bonus Histology Tech - Modesto, CA 
Mohs Tech - Naples, FL HT/HTL - Milwaukee, WI Dermpath Histotech - Kansas City, 
KS Histotech - Louisville, KY (learn electron microscopy) Dermpath 

[Histonet] Androgen receptor antibody raised against mouse

[Swartwood, Steven J via Histonet]

Hello all,

Does anyone have a recommendation for an anti-mouse Androgen Receptor antibody 
for Immunohistochemistry on formalin fixed paraffin embedded mouse tissues? We 
have some mouse prostate tumor models that we're working with. Again we are 
trying to stain for Androgen Receptor in a mouse, and not the human AR. I have 
reviewed a few papers and have some decent options, but I'd like to see if the 
histonet community has any expertise with this.

Thank you for all your time and efforts as you always help me out,

Steven Swartwood HT (ASCP)
Cedars-Sinai Medical Center
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responsible for delivering it to the intended recipient, you are hereby 
notified that any dissemination, distribution or copying of this information is 
strictly prohibited. Thank you for your cooperation.
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[Histonet] job in Boston area

[Caitlin Hesketh via Histonet]

Hi everyone!

I'm currently looking for a Histotech  - two shifts available, both early
morning, 8 hour, Tues-Saturday. Primarily someone to embed and cut,
opportunities to do specials and IHC staining as well. We're a small but
busy lab just outside Boston that does exclusively derm specimens. If
you're interested or know anyone who might be please email
caithesk...@gmail.com. Thank you!
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[Histonet] Thanks for assistance

[Lester Raff MD via Histonet]

Thanks for spreading the word about block disposal. I have been inundated with 
requests, will have to sort through them all!



For anyone interested Friday blog post 
http://www.chicagonow.com/downsize-maybe/2016/01/what-is-the-last-sound-you-will-hear/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] Histonet Digest, Vol 146, Issue 4 validation suggestions for short cycle processing with conventional equipment

[Steve McClain via Histonet]

I posted our 7 year experience on this topic previously on histonet.
Remember, this machine (K3000, VIP5) was not designed specifically for this 
purpose, but it can be made to work admirably.  
IOW Short processing on VIP is unreliable UNLESS you are willing to accept or 
control certain conditions, 
1) only process well-fixed tissue 
2) take formalin off this processor, making 14 changes beginning in 50%. If you 
want to keep station 1 formalin and standardize fixation with time X in 
formalin at temp Y, that may work. I prefer to change the tissues to 50% 
alcohol at the grossing bench so that all tissues are not only fixed, but 
rinsed in alcohol and started processing at the growing bench. This simplifies 
processing to dehydration, clearing and paraffin infiltration.  Simple is 
reliable and short processing cycles on this machine leave little room for 
error.
2) Program heat to 50C in all alcohols and xylene. Mixing pressure and vacuum 
yes yes yes 
4) Keep all solutions fresh, rotate after 200- 400 blocks.
5) Lay blocks out not more than 2 cassettes back to back, max 100 for VIP5.
4 hour is reliable for tissues up to 3mm thickness such as punch biopsy. 
2 hour is reliable for up to 1.5 mm tissue thickness such as core biopsies and 
shave biopsies of skin.

Method of processor validation for short cycle in machine previously validated 
in your lab.

To validate create your evaluation form and find a set of cases with sufficient 
tissue small tissue to divide into 2 equal blocks. This can (and will) be done 
on clinical cases if you are careful. 
Print duplicate sets of cassettes. 
Mark one half of case 1 yellow and one half blue (tissues will be processed 
separately, 1 regular and one short cycle and then recombined into 1 final 
block at embedding).
We held the short processed tissue cassettes in formalin and timed the short 
cycle to end when the regular program ends.
Then the two blocks are recombined or embedded together so that direct 
comparisons/measures can be made on one H and one on your most common IHC for 
that tissue. If the H and IHC both work, chances are everything else will 
also.
Modify your regular validation form or Create a form for scoring shrinkage, 
measured thickness and length, staining reactions, shrinkage, holes and flaws, 
have your path score.   photo graph yellow and blue tissues under H and IHC. 
IF you do observe shrinkage or other undesirable artifact, be certain that your 
tissues are completely fixed at grossing and repeat.

If they are comparable in the oculars and to the camera, then show your work 
and voila, there is your validation. 

Steve A. McClain, MD

> 

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[Histonet] lab humidifiers

[Sanders, Jeanine (CDC/OID/NCEZID) via Histonet]

Morning everyone!

What is the standard in humidity levels that most histology labs strive for? 
And to achieve this goal, do any of you use a commercial-grade humidifier?

Thanks for your help and have a nice weekend!


Jeanine H. Sanders
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS-G32
Atlanta, GA 30329

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[Histonet] Price gun

[Histology Technician via Histonet]

  I was thinking of using a price gun to date my supplies when they come in, 
but I can't find one to buy.  Any ideas where to search for one?What do you 
guys use to date your supplies?  I've always used a pen to write the rcd date 
and my initials but thought a price gun would be quicker and neater :)
Thanks!
___
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[Histonet] HPV 16/18 background

[Edison Narvaez via Histonet]

Hello,
I'm running HPV probes from Enzo on my Leica instruments, and we are noticing 
background episomal pattern on 16/18 staining of cases that are strongly 
positive for HPV 6/11, but not on cases that are 6/11 or 16/18 negative.
Is anybody experiencing this same issue and are there any suggestions.

I'm using Enzo probes, Enzo Stringency wash, Enzo mouse-antibiotin, and a 
polymer detection kit.

Thank you










The contents of this message, together with any attachments, are intended only 
for the use of the person(s) to which they are addressed and may contain 
confidential and/or privileged information. Further, any medical information 
herein is confidential and protected by law. It is unlawful for unauthorized 
persons to use, review, copy, disclose, or disseminate confidential medical 
information. If you are not the intended recipient, immediately advise the 
sender and delete this message and any attachments. Any distribution, or 
copying of this message, or any attachment, is prohibited.
___
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Re: [Histonet] lab humidifiers

[Michael Ann Jones via Histonet]

We are struggling with our humidity constantly.
Colorado is dry in the winter.
We use at least two humidifiers to try to keep humidity at minimum 30% due
to equipment manufacturer¹s specs.
We have log sheets and thermometer/humidifier measurers to keep track of
humidity.
Standard for equipment seems to be 30 - 80% humidity per manufacturer¹s
specifications of processors, etc.
Good luck 


Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

Providing collaborative diagnostic services,
saving lives today and tomorrow.






On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet"
 wrote:

>Morning everyone!
>
>What is the standard in humidity levels that most histology labs strive
>for? And to achieve this goal, do any of you use a commercial-grade
>humidifier?
>
>Thanks for your help and have a nice weekend!
>
>
>Jeanine H. Sanders
>Centers for Disease Control and Prevention
>1600 Clifton Rd., NE MS-G32
>Atlanta, GA 30329
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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[Histonet] Happy New Year! Here's to a Fantastic 2016!

[Pam Barker via Histonet]

Hi Histonetters!
Happy New Year
Here's to a terrific 2016!!!
How did you ring in the New Year? 
I hope you had fun ushering out 2015 and welcoming in 2016.  
I know I am looking forward to an exciting new year.
 
I wanted to drop you this quick line to let you know that I have been
chatting with clients and have some exciting new opportunities.  
Here is a list of my current open positions:
Leadership:
Histology Lab Manager - Modesto, CA
Histology Supervisor (Dermpath) - Fayetteville, AR
Senior Histotech - Norfolk, VA  B.S. required

Histotechnicians/Histotechnologists:
Histology Tech - Norfolk, VA 15K Sign on Bonus
Histology Tech - Modesto, CA
Mohs Tech - Naples, FL
HT/HTL - Milwaukee, WI 
Dermpath Histotech - Kansas City, KS
Histotech - Louisville, KY (learn electron microscopy) 
Dermpath Histotech - Tyler, TX
Histotech - San Diego, CA 
Tech Support - Los Angeles, CA 
 
All of these are full time positions and my clients offer excellent
compensation, benefits and in most cases relocation assistance and or a sign
on bonus.  
For more information please contact me by e-mail: rel...@earthlink.net  or
toll free: 866-607-3542 or you can reach me on my cell/text: 407-353-5070.
 
If none of these positions interest you and you are on the hunt for a new
position NOW please drop me an email and let me know what you are looking
for.  My phone is ringing off the hook and your next opportunity could be
just a phone call away.  When I get the call I want to know to call YOU!
 
If you are set in your current position Congratulations! and thank you for
taking the time to read my email.  
I have new positions coming in all of the time and you never know when
something might interest you or a friend or coworker.  
The best part is if you refer someone and I place them you will earn a
referral fee!  
So please stay tuned for my next email!  
 
Happy New Year to You & Yours!!  
I wish you a year of love, laughter, good health & prosperity.

Thanks-Pam

Happy New Year!

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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Re: [Histonet] lab humidifiers

[Sanders, Jeanine (CDC/OID/NCEZID) via Histonet]

Thanks. We dog log the temp. I have had several service techs come in and 
comment on how much static we have. It has been worse than usual lately so am 
looking into options. Thanks much for your input!


From: Michael Ann Jones [mjo...@metropath.com]
Sent: Friday, January 08, 2016 10:01 AM
To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] lab humidifiers

We are struggling with our humidity constantly.
Colorado is dry in the winter.
We use at least two humidifiers to try to keep humidity at minimum 30% due
to equipment manufacturer¹s specs.
We have log sheets and thermometer/humidifier measurers to keep track of
humidity.
Standard for equipment seems to be 30 - 80% humidity per manufacturer¹s
specifications of processors, etc.
Good luck


Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com

Providing collaborative diagnostic services,
saving lives today and tomorrow.






On 1/8/16, 6:26 AM, "Sanders, Jeanine (CDC/OID/NCEZID) via Histonet"
 wrote:

>Morning everyone!
>
>What is the standard in humidity levels that most histology labs strive
>for? And to achieve this goal, do any of you use a commercial-grade
>humidifier?
>
>Thanks for your help and have a nice weekend!
>
>
>Jeanine H. Sanders
>Centers for Disease Control and Prevention
>1600 Clifton Rd., NE MS-G32
>Atlanta, GA 30329
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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