[Histonet] Knife holder

[Martin, Gary via Histonet]

I'm looking for a high profile knife holder for my Micron HM 325
microtome, does anyone know where I might get hold of this part. 

Thanks

Gary 

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Re: [Histonet] Histonet Digest, Vol 148, Issue 6

[Douglas Gregg via Histonet]

Message: 5
Date: Mon, 7 Mar 2016 12:50:51 -0500
From: Charles Riley 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] DI water
Message-ID:

Re: [Histonet] DI water

[Caroline Miller via Histonet]

We use this one:
http://smile.amazon.com/Pure-Stainless-Counter-Distiller-Secure/dp/B003ZJRAWO/ref=pd_sim_sbs_60_12?ie=UTF8=1A4WVXEGE3W42HHT72B0

It has worked so far for our histology applications and our microscopes
waterbath, but I would use 'good' purchased water for any molecular biology
applications. Sigma has some.

You can also get a good deal on 5gallon jugs from mcmastercarr (
http://www.mcmaster.com/#distilled-water/=11fsnq8) of both distilled and
deionized water (about $25 each) - but they cost a lot of money (and
pollution) to deliver. That being said I do not know the carbon footprint
of my distillation machine.

Oh, how I miss my millipore system, but OH MY they cost a lot of money to
buy, install and fix when they go wrong. Too much for this little start up.
Our usage does not afford that luxury!

yours,
mills

On Mon, Mar 7, 2016 at 9:50 AM, Charles Riley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Does anyone know of a cheap way to make your own DI water? Or a good
> reliable company in the Delaware area that provides DI water services?
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
>
> Doctors Pathology Services, Dover DE
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Microscopic slides

[Walter Benton via Histonet]

Try Globe and Tanner slides, both should work well in your slidemate.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Vickroy, James via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, March 07, 2016 1:33 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Microscopic slides


We are once again reviewing the microscopic slides we use.  We have found the 
sldies we have used on a regular basis take too long to dry properly which 
gives us some undesireable artifacts.  I have longed for a "one size fits all" 
microscopic slide.One of the slides I used in the past is not possible 
given that I have the Thermofisher Slidemates.   The slidemates require a very 
smooth labeled end.   Recently we tested several slides both hydrophobic and 
hydrophilic.  We really liked a hydrophilic slide by Leica but am told it 
doesn't work well for IHC's and special stains.   Our current slide is a 
"hydrophobic" slide which traps water easily underneath the section and 
therefore we having drying problems.  If we don't dry them for at least twenty 
minutes we get nuclear bubbling.  Leica has another slide we tried that is 
"hydrophobic" but with "hydrophilic" tendencies.  My staff thought tried that 
slide and found them to be very similar to the "hydrophobic" slide we already 
have.

We have tried the Stat-lab M2000 and they seem to  dry better but there often 
is a "trick" to getting the sections to stick to the slide without sliding off 
of the end.Of course we might just have to use two different slides, one 
for our routine H's and one for the IHC's and Special stains.   Anybody have 
any thoughts on this?

And is a "hydrophobic slide" that has "hydrophilic tendencies" really possible?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
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[Histonet] Microscopic slides

[Vickroy, James via Histonet]

We are once again reviewing the microscopic slides we use.  We have found the 
sldies we have used on a regular basis take too long to dry properly which 
gives us some undesireable artifacts.  I have longed for a "one size fits all" 
microscopic slide.One of the slides I used in the past is not possible 
given that I have the Thermofisher Slidemates.   The slidemates require a very 
smooth labeled end.   Recently we tested several slides both hydrophobic and 
hydrophilic.  We really liked a hydrophilic slide by Leica but am told it 
doesn't work well for IHC's and special stains.   Our current slide is a 
"hydrophobic" slide which traps water easily underneath the section and 
therefore we having drying problems.  If we don't dry them for at least twenty 
minutes we get nuclear bubbling.  Leica has another slide we tried that is 
"hydrophobic" but with "hydrophilic" tendencies.  My staff thought tried that 
slide and found them to be very similar to the "hydrophobic" slide we already 
have.

We have tried the Stat-lab M2000 and they seem to  dry better but there often 
is a "trick" to getting the sections to stick to the slide without sliding off 
of the end.Of course we might just have to use two different slides, one 
for our routine H's and one for the IHC's and Special stains.   Anybody have 
any thoughts on this?

And is a "hydrophobic slide" that has "hydrophilic tendencies" really possible?

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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[Histonet] DI water

[Charles Riley via Histonet]

Does anyone know of a cheap way to make your own DI water? Or a good
reliable company in the Delaware area that provides DI water services?

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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Re: [Histonet] Whale skin

[Rene J Buesa via Histonet]

How did you manage to deal with the about 0.5 m of blubber?Was it the skin of a 
new born whale? I just to not understand, but you have to completely eliminate 
all the fat and increase your processing protocol (infiltration specially) to 
have some chance of getting any relatively "decent" section.René 

On Monday, March 7, 2016 11:23 AM, Kathleen Jones via Histonet 
 wrote:
 

 Hello, 

I have to cut FFPE blocks of whale skin for in IHC study. I am having
difficulty with both cutting and floating. Any advice would be much
appreciated!

Thank you!





Kathleen Jones
Research Technologist
Pathology/Microbiology
AVC - UPEI


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[Histonet] Whale skin

[Kathleen Jones via Histonet]

Hello, 

I have to cut FFPE blocks of whale skin for in IHC study. I am having
difficulty with both cutting and floating. Any advice would be much
appreciated!

Thank you!





Kathleen Jones
Research Technologist
Pathology/Microbiology
AVC - UPEI


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[Histonet] Blog post--lab related

[Lester Raff MD via Histonet]

http://www.chicagonow.com/downsize-maybe/2016/03/no-lovie-for-our-lab/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Florida Society for Histotechnology Spring Meeting

[John Shelley via Histonet]

Hi All,

Happy Monday!

I would like to inform all histonetters both in Florida and abroad that our 
meeting program has been finalized and registration is now open. We have a 
wonderful array of workshops that will appeal to all levels of experience. We 
have our 3rd annual All-Day Supervisor and Administrator Forum on Friday, May 
20th, 2016 along with our All-Day HT Preparedness Workshop for students and 
those who are looking for a fresher on Saturday, May 21st, 2016. Along with 
general histology, safety, IHC, molecular, digital imaging and much more. 
Please use links below to look at our program and then our links for 
registration to the meeting and hotel.

FSH Online Registration

FSH Online 
Program

FSH Hotel 
Registration

Look forward to seeing you all in sunny and warm St. Pete., Florida at the 
Bayfront Hilton on May 19-22, 2016.

Kind Regards!

John J Shelley
2014-2016 FSH President

Histology Core Manager
Sanford Burnham Prebys Medical Discovery Institute at Lake Nona

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