Re: [Histonet] No More Blog Posts -- Over and Out!

[WILLIAM DESALVO via Histonet]

Another personal e-mail from you that is unwanted. I am feeling like I have my 
own stalker. You are one of the reasons that individuals will not post or 
comment, personal and ugly. I cannot imagine why your workplace (LUMC.edu) 
chose you to represent them. I request that you no longer contact me and make 
personal attacks. I know nothing of you and you certainly do not know anything 
about me. If you have something to say, post to the listserve, not me.

> From: spinhe...@lumc.edu
> To: wdesalvo@outlook.com
> Subject: RE: [Histonet] No More Blog Posts --  Over and Out!
> Date: Mon, 2 May 2016 20:15:38 +
> 
> As is yours. Just can't keep that dictatorial aspect out of your charming 
> consultant two cents worth.
> 
> Steve Pinheiro
> 
> 
> -Original Message-
> From: WILLIAM DESALVO via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Monday, May 02, 2016 2:11 PM
> To: Manfre, Philip; Rene J Buesa
> Cc: 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
> 
> Your response is WAY out of line. Keep it professional.
> 
> Sent from my Windows Phone
> 
> From: Manfre, Philip via Histonet
> Sent: ‎5/‎2/‎2016 12:08 PM
> To: Rene J Buesa
> Cc: 
> 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts --  Over and Out!
> 
> Delete button broken, huh.  I suppose it was necessary to attack a colleague 
> to feel puffed up about yourself.  Bully for you!
> 
> -Original Message-
> From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Monday, May 02, 2016 2:23 PM
> To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] No More Blog Posts -- Over and Out!
> 
> Thank you VERY MUCH!René
> 
> On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
>  wrote:
> 
> 
>  To My Lab Colleagues:
> 
> As my intent has never been to sow discontent or rancor, I think it is for 
> the best if I no longer post links to my blog, lab related or otherwise.  Of 
> course the blogs go on, and if anyone is interested in being added to my 
> mailing list for future notifications, just drop me a line at 
> les.r...@post.com  The mailing list is never used 
> for any purpose other than announcing a new blog post. Be sure to let me know 
> you are from the Histonet list!
> 
> I will continue to participate in any histology/pathologist discussions here 
> as I have for many years.
> 
> Cheers,
> 
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
> 
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> 
> 
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Re: [Histonet] No More Blog Posts -- Over and Out!

[Caroline Miller via Histonet]

Thank you Lester, I really appreciate your decision, and I look forward to
reading your histology-related histonet posts.

yours,
mills

On Mon, May 2, 2016 at 10:39 AM, Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> To My Lab Colleagues:
>
> As my intent has never been to sow discontent or rancor, I think it is for
> the best if I no longer post links to my blog, lab related or otherwise.
> Of course the blogs go on, and if anyone is interested in being added to my
> mailing list for future notifications, just drop me a line at
> les.r...@post.com   The mailing list is never
> used for any purpose other than announcing a new blog post. Be sure to let
> me know you are from the Histonet list!
>
> I will continue to participate in any histology/pathologist discussions
> here as I have for many years.
>
> Cheers,
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] No More Blog Posts -- Over and Out!

[Boyd, Debbie M via Histonet]

I just feel if you didn't care for the subject/blog (doesn't pertain to you, 
you don't have any exposure to it etc. ) you could have deleted it without 
being so mean spirited.  I have in the past ignored the mean spirited sparring, 
but this was a bit much.
Whatever happened to kindness?
Just my two cents

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: Manfre, Philip via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:38 PM
To: Rene J Buesa
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] No More Blog Posts --  Over and Out!

Delete button broken, huh.  I suppose it was necessary to attack a colleague to 
feel puffed up about yourself.  Bully for you!

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you VERY MUCH!René

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:


 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com  The mailing list is never used for 
any purpose other than announcing a new blog post. Be sure to let me know you 
are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] No More Blog Posts -- Over and Out!

[WILLIAM DESALVO via Histonet]

Your response is WAY out of line. Keep it professional.

Sent from my Windows Phone

From: Manfre, Philip via Histonet
Sent: ‎5/‎2/‎2016 12:08 PM
To: Rene J Buesa
Cc: 
'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts --  Over and Out!

Delete button broken, huh.  I suppose it was necessary to attack a colleague to 
feel puffed up about yourself.  Bully for you!

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you VERY MUCH!René

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:


 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com  The mailing list is never used for 
any purpose other than announcing a new blog post. Be sure to let me know you 
are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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New Jersey, USA 07033), and/or its affiliates Direct contact information
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proprietary copyrighted and/or legally privileged. It is intended solely
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Re: [Histonet] IHC positive controls

[Morken, Timothy via Histonet]

Cynthia, best practice is to keep in the block and cut as needed. Second is to 
cut and dry at room temp and not melt, then store. Don't cut, melt and store. 
The issue is oxidation. Keeping in paraffin prevents oxidation. You'll protect 
the antigens better if they  are fully isolated from air, which paraffin does, 
but once cut, they are exposed. That happens in the fridge as well and I'm not 
sure refrigeration helps much. I'm guessing that you don't do a lot of these, 
so you don't want to cut a lot of slide and have them sitting around for months 
waiting for use. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Cindy Bulmer via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 02, 2016 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls


Hello Histoland,
 
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
 
1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) for 
1 hr. 
 then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and put 
slides
 directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.
 
Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX




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Re: [Histonet] No More Blog Posts -- Over and Out!

[Manfre, Philip via Histonet]

Delete button broken, huh.  I suppose it was necessary to attack a colleague to 
feel puffed up about yourself.  Bully for you!

-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, May 02, 2016 2:23 PM
To: Lester Raff MD; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] No More Blog Posts -- Over and Out!

Thank you VERY MUCH!René 

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:
 

 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com  The mailing list is never used for 
any purpose other than announcing a new blog post. Be sure to let me know you 
are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
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for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
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[Histonet] Fwd:

[Linda Margraf via Histonet]

> From: Cindy Bulmer 
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
> 
> Hello Histoland,
>  
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>  
> 1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) 
> for 1 hr.
>  then put slides in refrigerator for future use.
> 2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and 
> put slides
>  directly in refrigerator for future use.
> 3)  Cut "fresh" every time they order the Ab.
>  
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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Re: [Histonet] No More Blog Posts -- Over and Out!

[Rene J Buesa via Histonet]

Thank you VERY MUCH!René 

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:
 

 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com  The mailing list is never used for 
any purpose other than announcing a new blog post. Be sure to let me know you 
are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] IHC positive controls

[Cindy Bulmer via Histonet]

Hello Histoland,
 
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
 
1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) for 
1 hr. 
 then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and put 
slides
 directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.
 
Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX




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[Histonet] No More Blog Posts -- Over and Out!

[Lester Raff MD via Histonet]

To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com   The mailing list is never used 
for any purpose other than announcing a new blog post. Be sure to let me know 
you are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] PLA2R assay question

[Martha Ward-Pathology via Histonet]

I have been asked to look into offering PLA2R on our renal biopsies and in 
doing some research on the subject I find that some people are staining frozen 
tissue via an indirect IMF procedure and some are staining ffpe tissues, also 
using an indirect IMF procedure.I would prefer to do this via traditional 
IHC (we have Bond 2 stainers).Is anyone doing this way?   If so, could you 
provide some tips on getting it to work successfully?

Thanks in advance for any help.


 
Martha Ward, MT (ASCP) QIHC
Manager
 
Molecular Diagnostics Lab
Medical Center Boulevard  \  Winston-Salem, NC 27157
p 336.716.2109  \  f 336.716.5890  
mw...@wakehealth.edu  


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[Histonet] open position

[Margiotta-Watz, Michele via Histonet]

Hi All,
We have an open position for a Histotech since one of my techs is retiring.  
This is a full-time position at a small community Hospital on eastern Long 
Island, NY.  We are looking for an experienced tech to work early morning hours 
Monday thru Friday and
no holidays.  If interested, please e-mail your resume to me at  
mmargio...@bmhmc.org or call me at the # below.

Thanks,
Michele Margiotta-Watz
Histology Supervisor
BMHMC
101 Hospital Road
Patchogue, NY 11772
631-654-7192

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