[Histonet] Pap stain without xylene
René and other Histonetters, After reading the paper: Buesa RJ, Peshkov, MV. Histology without xylene. Ann Diagn Pathol. 2009 Aug;13(4):246-56. Epub 2009 Feb 5. It appears that xylene in the final clearing steps is replaced with isopropanol and mineral oil as follows: ·5:1 isopropanol to mineral oil 50°C ·2:1 isopropanol to mineral oil 50°C ·Undiluted mineral oil 50°C ·Drying oven 5 minutes 60°C ·Coverslip Do you feel these clearing steps be applied to the pap stain in order to eliminate xylene? If so, can it be done at room temperature? Thanks, Mike Mike Toole, BS, CT(ASCP)CM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Employment Opportunity: Cold Spring Harbor Laboratories
Research Investigator/ Director, Tissue Imaging at the Cancer Centerof Cold Spring Harbor Laboratory Job SummaryThe Tissue ImagingTeam processes and generates all histology analysisfor CSHL’s basic and preclinical cancer research. The team processesanimal and human tissuehistology and develops state of the art histology visualization.We are seeking a highly motivatedresearch scientist to head the team and run automatedantibody applications. Key Qualifications• Extensive experience with automated histologystaining in fieldsof medical, health or developmental biology.• Extensive experience with experimental design,development and execution of immunohistochemistry at core facilityor equivalent setting• Experience with administration and budgeting at a core facility• Experience with troubleshooting and maintaining automated histology equipment. DescriptionKey responsibilities include: Lead a three person team dedicatedto histology services. Develop and streamline the team’s histology workflow from tissueembedding to digital archivingand sharing of virtual slides.Design, troubleshooting and optimization of all immunohistology staining using automated slide staining system. Responsibility for providing reliabledata for validation of research hypotheses. Close collaboration with variouscancer research groups. EducationDegree: Ph.D., M.D. or D.V.M., Master’smay be considered given exceptional experience.Field of Study: Clinical,Pre-clinical medical research or developmental biology Additional RequirementsExcellent communication and interpersonal skills. Strong analytical and bench problem-solving skills. Must thrive in fast-paced environment and keep abreast of relevantdevelopments in the field. Must be highlyorganized, and able to multitask. Demonstrated abilityto work as a key member of a team. Please send your materials todts...@cshl.edu Cold SpringHarbor Laboratory is recognized internationally for its excellence in research and educational activities and support innovative research programs in cancer, neuroscience, plant biology, quantitative biology and genomics. Additional information about CSHL and the Watson Schoolcan be obtained at www.cshl.edu. ColdSpring Harbor Laboratory is an equal opportunity employer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Question - CoPath
Make that "Sunquest" CoPath .. Richard -Original Message- From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question - CoPath We will be switching from Cerner CoPath to Sunrise CoPath in the near future (part of our Epic conversion). Is there anyone using Sunrise CoPath that uses a prostate diagram in their report to demonstrate the absence or presence of cancer in the different quadrants? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question - CoPath
We will be switching from Cerner CoPath to Sunrise CoPath in the near future (part of our Epic conversion). Is there anyone using Sunrise CoPath that uses a prostate diagram in their report to demonstrate the absence or presence of cancer in the different quadrants? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GHS vs. NFPA labeling
See the link below for a recent blog entry addressing this very question. I do not believe placing a new label on an original container is not permitted. I would transfer to a secondary container, if able, and label accordingly. http://danthelabsafetyman.tumblr.com/ Thanks, Julia Cates, HT(ASCP)cm Pathology Coordinator, Pathology Park Ridge Health Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] GHS vs. NFPA labeling
I believe you can purchase the new GHS labels and just affix them to your reagents that don't already have them. Jeanne Blanchard, CT (ASCP)CM -Original Message- From: Fortune, James A. via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GHS vs. NFPA labeling So I have a question about the labelling of chemicals. I understand that new liquid chemicals must have the GHS labels, and I noticed some of our dry chemicals also have the new GHS labelling. What about old dyes and other dry chemicals that are still good but do not have the GHS labels but have the old NFPA labels? As most of you know dyes are expensive and most are very old and still work great. So I want to clarify this before we go crazy and start trashing dry chemicals and dyes. Any input would be greatly appreciated. Andy Fortune Tech Specialist Meriter Laboratories Madison, WIi This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hoods
We had an inspection from the fire department recently and they said we need to have the exhaust from the fume hood checked annually. Does anyone know who does that? The people that do our P.M. on our machines say they don't, so who would? Thanks Anne ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibody MyD88
Does anyone have a protocol using the Ventana Ultra for this antibody? Thanks in advance. Rae Staskiewicz UnityPoint Health-Methodist/Proctor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
