Re: [Histonet] Marking Tissues with Eosin

[Cindy Bird via Histonet]

We place a small drop of concentrate straight on tissue.

Sent from my iPhone

> On Jun 23, 2016, at 12:56 PM, Mca Werdler via Histonet 
>  wrote:
> 
> Dear Rebecca,
> 
> Yes this is possible. just don't use a too strong concentration. The eosin
> should give a slight pink color on the tissue after processing and after
> embedding.
> 
> Good luck,
> 
> Maarten
> 
> 2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
> histonet@lists.utsouthwestern.edu>:
> 
>> I had a biopsy today that was nearly impossible to see on the sponges
>> during embedding or in the block.  I've heard mention of marking these with
>> eosin to make them easier to see.  Has anyone done this?  Or do you use
>> some other type of marking dye for this purpose?
>> Thanks for your input!
>> Rebecca
>> 
>> Rebecca Ashley
>> Histotechnologist
>> Wyoming State Vet Lab
>> 1174 Snowy Range Rd.
>> Laramie, WY 82070
>> Phone: 307-766-9946
>> Fax: 307-721-2051
>> 
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Shandon Varistain Gemini Slide Stainer

[Paula Keene Pierce via Histonet]

This is so true!
I had a Fisher Histomatic automated slide stainer that was manufactured in 1985 
that I used until the building took a direct lightning strike JUST LAST YEAR!
My old VIP is still going!
 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com

  From: Teri Johnson via Histonet 
 To: "histonet@lists.utsouthwestern.edu"  
 Sent: Thursday, June 23, 2016 2:04 PM
 Subject: Re: [Histonet] Shandon Varistain Gemini Slide Stainer
   
Hi Thomas,

Are you looking for true life expectancy or what is reported for depreciation? 
In my experience, most tissue processors never die, but only need to be retired 
due to lack of available support/parts or because a lab requires newer 
technology.

Also in my experience, a "better" model comes out soon after I have purchased 
one. So the life span is probably 20 years after you wished you had a different 
one. :-)

Best wishes,

Teri

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com

--

Hi All,
Does anyone know the life expectancy of the Gemini H stainer? My boss has 
asked me this question because we are in the process of requesting for a new 
one.

Thomas

Thomas Huynh  BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | 
thomas.hu...@harrishealth.org

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender 
by return e-mail and delete this e-mail and any attachments from your computer 
system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability and 
Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or 
Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under Texas 
law.  The e-mail is for the use of only the individual or entity named above.  
If you are not the intended recipient, or any authorized representative of the 
intended recipient, you are hereby notified that any review, dissemination or 
copying of this e-mail and its attachments is strictly prohibited.






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Marking Tissues with Eosin

[Jamal Rowaihi via Histonet]

Hi If you are using buffered Formaline so the Eosin color will not resist until 
the end of tissue processing.I recommend to add small amount of stock Eosin to 
the last alcohol in theTissue processor. 

Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone
 Original message From: Mca Werdler via Histonet 
 Date: 6/23/16  8:49 PM  (GMT+03:00) To: 
"Rebecca E. Ashley"  Cc: Histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] Marking Tissues with Eosin 
Dear Rebecca,

Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.

Good luck,

Maarten

2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet@lists.utsouthwestern.edu>:

> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block.  I've heard mention of marking these with
> eosin to make them easier to see.  Has anyone done this?  Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Shandon Varistain Gemini Slide Stainer

[Teri Johnson via Histonet]

Hi Thomas,

Are you looking for true life expectancy or what is reported for depreciation? 
In my experience, most tissue processors never die, but only need to be retired 
due to lack of available support/parts or because a lab requires newer 
technology.

Also in my experience, a "better" model comes out soon after I have purchased 
one. So the life span is probably 20 years after you wished you had a different 
one. :-)

Best wishes,

Teri

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com

--

Hi All,
Does anyone know the life expectancy of the Gemini H stainer? My boss has 
asked me this question because we are in the process of requesting for a new 
one.

Thomas

Thomas Huynh   BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 | 
thomas.hu...@harrishealth.org

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the sender 
by return e-mail and delete this e-mail and any attachments from your computer 
system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability and 
Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or 
Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under Texas 
law.  The e-mail is for the use of only the individual or entity named above.  
If you are not the intended recipient, or any authorized representative of the 
intended recipient, you are hereby notified that any review, dissemination or 
copying of this e-mail and its attachments is strictly prohibited.






___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Marking Tissues with Eosin

[Mca Werdler via Histonet]

Dear Rebecca,

Yes this is possible. just don't use a too strong concentration. The eosin
should give a slight pink color on the tissue after processing and after
embedding.

Good luck,

Maarten

2016-06-23 12:04 GMT-05:00 Rebecca E. Ashley via Histonet <
histonet@lists.utsouthwestern.edu>:

> I had a biopsy today that was nearly impossible to see on the sponges
> during embedding or in the block.  I've heard mention of marking these with
> eosin to make them easier to see.  Has anyone done this?  Or do you use
> some other type of marking dye for this purpose?
> Thanks for your input!
> Rebecca
>
> Rebecca Ashley
> Histotechnologist
> Wyoming State Vet Lab
> 1174 Snowy Range Rd.
> Laramie, WY 82070
> Phone: 307-766-9946
> Fax: 307-721-2051
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Marking Tissues with Eosin

[Rebecca E. Ashley via Histonet]

I had a biopsy today that was nearly impossible to see on the sponges during 
embedding or in the block.  I've heard mention of marking these with eosin to 
make them easier to see.  Has anyone done this?  Or do you use some other type 
of marking dye for this purpose?
Thanks for your input!
Rebecca

Rebecca Ashley
Histotechnologist
Wyoming State Vet Lab
1174 Snowy Range Rd.
Laramie, WY 82070
Phone: 307-766-9946
Fax: 307-721-2051

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Shandon Varistain Gemini Slide Stainer

[Huynh, Thomas via Histonet]

Hi All,
Does anyone know the life expectancy of the Gemini H stainer? My boss has 
asked me this question because we are in the process of requesting for a new 
one.

Thomas

Thomas Huynh   BS, HT (ASCP)
Histology Lab Supervisor |Department of Pathology
HARRISHEALTH SYSTEM
5656 Kelly Street
Houston, Tx 77026
Lyndon B. Johnson General Hospital (LBJGH)
O: 713.566.5282 | F: 713.566.5285 | P: 713.297.1606 |
thomas.hu...@harrishealth.org

CONFIDENTIALITY NOTICE:
If you have received this e-mail in error, please immediately notify the
sender by return e-mail and delete this e-mail and any attachments from 
your computer system.

To the extent the information in this e-mail and any attachments contain 
protected health information as defined by the Health Insurance Portability 
and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 
164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or 
privileged.  This e-mail may also be confidential and/or privileged under 
Texas law.  The e-mail is for the use of only the individual or entity named 
above.  If you are not the intended recipient, or any authorized 
representative of the intended recipient, you are hereby notified that any 
review, dissemination or copying of this e-mail and its attachments is 
strictly prohibited.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet