[Histonet] Feedback on Immunohistochemistry stainers

2016-07-28 Thread Gmail via Histonet 

Hi all,
We are looking into getting a new staining platform for our IHC lab. I would 
appreciate any feedback from your experience regarding ease of use, how long 
maintenance;daily , monthly takes to perform and through put. Are there any 
issues you ran into ? We run approximately 4-6000 slides/ month and are looking 
into Roche, Leica and Dako.
Thanks in advance


Andrea Beharry
Senior MLT, Immunohistochemistry 
William Osler Health System




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Re: [Histonet] It's getting cold in here....

2016-07-28 Thread Morken, Timothy via Histonet 
Seems like nurses would have enough to do already!  I wonder if this is aimed 
at physician labs where they may have a nurse but no lab tech...

California requirement for RN BS degree require only  one quarter of 
microbiology with lab, and one quarter of basic chemistry with lab. And 3 
anatomy/physiology quarter courses

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


Tim


-Original Message-
From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 28, 2016 12:06 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] It's getting cold in here

If you've not been made aware before now, be warned.  The Department of 
Veterans affairs (DVA) has proposed a rule that would authorize any Advanced 
Practice Registered Nurse (APRN) to not only order and interpret lab tests, but 
also to perform, supervise, and direct lab testing.  The rule follows lab 
personnel requirement changes enacted by the Centers for Medicare and Medicaid 
Services (CMS) that say that a bachelor's or associate's degree in nursing is 
sufficient  to perform high complexity lab testing.
With a nod to nursing degrees that I'm sure prepare a nurse extremely well for 
nursing, there is no way that a nursing degree can adequately prepare a person 
to perform complex laboratory testing.  Unless your state has technologist 
licensure, and few do,  get prepared for nurses running the lab, and lab tests. 
 And where will that leave the histologist technician?  I'm guessing out in the 
cold.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
**


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[Histonet] It's getting cold in here....

2016-07-28 Thread Terri Braud via Histonet 
If you've not been made aware before now, be warned.  The Department of 
Veterans affairs (DVA) has proposed a rule that would authorize any Advanced 
Practice Registered Nurse (APRN) to not only order and interpret lab tests, but 
also to perform, supervise, and direct lab testing.  The rule follows lab 
personnel requirement changes enacted by the Centers for Medicare and Medicaid 
Services (CMS) that say that a bachelor's or associate's degree in nursing is 
sufficient  to perform high complexity lab testing.
With a nod to nursing degrees that I'm sure prepare a nurse extremely well for 
nursing, there is no way that a nursing degree can adequately prepare a person 
to perform complex laboratory testing.  Unless your state has technologist 
licensure, and few do,  get prepared for nurses running the lab, and lab tests. 
 And where will that leave the histologist technician?  I'm guessing out in the 
cold.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
**


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Re: [Histonet] Distilled Water vs DI water for IHC and H staining

2016-07-28 Thread Caroline Miller via Histonet 
Totally agree with the other comments, I had one anecdote though. I worked
in a clinical lab at the Hammersmith in London, with an autostainer. One
day the H started coming off really funny, way too blue, not enough
eosin. We traced everything in the lab, and NOTHING had changed. We
scratched our heads, banged them against the wall and fixed the problem by
changing staining times and fiddling with the protocol.

JUST as we got it fixed the powers that be told us the whole hospital had
changed our main water supply!

Oh, histology, you are so voodoo sometimes!

Happy Friday!

mills

On Thu, Jul 28, 2016 at 9:54 AM, Morken, Timothy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Charles, for most histology applications water from laboratory-grade DI
> and distilled systems are fairly interchangeable because modern systems
> bring them pretty close to the same purity. But some stains can be affected
> if they are susceptible to chemical interactions with something left in the
> water. That is probably more important for tests like enzyme histochemistry
> than bulk chemical dye staining.
>
> These systems are not just stand-alone "deionized" or "distilled" anymore
> and now include various pre- and post- filtration steps for removing
> particulates, volatile compounds, bacteria, etc  (ours "DI" system has
> several DI resin beds, carbon filters, particulate filters, UV etc). Even
> modern distilling systems will filter tap water with various filters
> (particulates, carbon, bacteria) before distilling.
>
> I don't think the consumer type filters will do the same job as a DI or
> distilled system. They are designed to filter out only the particulates and
> volatile compounds (particulate/bacteria and carbon filters for the most
> part). There is no deionization or demineralization (assuming you are using
> city tap water). However, a reverse osmosis system (which also has several
> other filters pre-RO) may do the trick for you. It is not quite as good at
> purifying as a laboratory-quality DI or Distilled system, but may suffice
> for histology.
>
>
> Tim Morken
> Pathology Site Manager, Parnassus
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
>
>
>
>
> -Original Message-
> From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu
> ]
> Sent: Thursday, July 28, 2016 4:54 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Distilled Water vs DI water for IHC and H staining
>
> Does anyone know if there is a difference in staining results when using
> DI water versus Distilled water? Our lab is trying to get away from the DI
> system we have as it is becoming expensive to maintain and we were looking
> to see if we could just use a home drinking filter with UV lighting for our
> water supply. Please let me know any thoughts or concerns you might have
> with this idea
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
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>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Distilled Water vs DI water for IHC and H staining

2016-07-28 Thread Morken, Timothy via Histonet 
Charles, for most histology applications water from laboratory-grade DI and 
distilled systems are fairly interchangeable because modern systems bring them 
pretty close to the same purity. But some stains can be affected if they are 
susceptible to chemical interactions with something left in the water. That is 
probably more important for tests like enzyme histochemistry than bulk chemical 
dye staining. 

These systems are not just stand-alone "deionized" or "distilled" anymore and 
now include various pre- and post- filtration steps for removing particulates, 
volatile compounds, bacteria, etc  (ours "DI" system has several DI resin beds, 
carbon filters, particulate filters, UV etc). Even modern distilling systems 
will filter tap water with various filters (particulates, carbon, bacteria) 
before distilling. 

I don't think the consumer type filters will do the same job as a DI or 
distilled system. They are designed to filter out only the particulates and 
volatile compounds (particulate/bacteria and carbon filters for the most part). 
There is no deionization or demineralization (assuming you are using city tap 
water). However, a reverse osmosis system (which also has several other filters 
pre-RO) may do the trick for you. It is not quite as good at purifying as a 
laboratory-quality DI or Distilled system, but may suffice for histology. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 28, 2016 4:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Distilled Water vs DI water for IHC and H staining

Does anyone know if there is a difference in staining results when using DI 
water versus Distilled water? Our lab is trying to get away from the DI system 
we have as it is becoming expensive to maintain and we were looking to see if 
we could just use a home drinking filter with UV lighting for our water supply. 
Please let me know any thoughts or concerns you might have with this idea

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] paraffin temp-processing

2016-07-28 Thread Noelle Linke via Histonet 
Hi all,

We are using Parapast X-tra on the Peloris at 65C (factory default), and I am 
thinking this is just way too hot (X-tra has a melting point of 52C).  Leica of 
course will not commit to an opinion.  Is anyone else using paraplast x-tra on 
their Peloris?

Thank you,
Noëlle

Noëlle Linke, MS, HTL(ASCP) QIHC
Manager, Anatomic Pathology
Pacific Diagnostic Laboratories
nli...@sbch.org
Phone: (805) 324-9814
Fax: (805) 696-6433






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[Histonet] Job open

2016-07-28 Thread Eunice via Histonet 
Swedish Covenant hospital in Chicago has a 20 hr evening   position open from 
6pm to 10pm mon to fri ... And possibly another every other Saturday open  
430am to 100pm please contact Ruth Cazares at 773 878 8200 ex 5190 

Sent from my iPhone
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Re: [Histonet] Distilled Water vs DI water for IHC and H staining

2016-07-28 Thread Wheelock, Timothy R. via Histonet 
Hi Charles:

Many years ago I was using deionized water to do the Bielschowsky silver stain.
The stains were coming out way too dark, with black-brown precipitate on the 
sections.
When I inherited a double-distilled water filtration system, that problem 
disappeared.
However, silver stains tend to be sensitive to all sorts of things.
I don't know if the same holds true for immunostains, since I don't do them 
myself (another lab does them for us).
When I do my Luxol Fast Blue (myelin)-Hematoxylin-Eosin stain, I just use tap 
water or all my washes.
I make my solutions for the stain with double-distilled water, but I am not 
sure if that is necessary.
I hope that this helps.

Tim

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 28, 2016 7:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Distilled Water vs DI water for IHC and H staining

Does anyone know if there is a difference in staining results when using DI 
water versus Distilled water? Our lab is trying to get away from the DI system 
we have as it is becoming expensive to maintain and we were looking to see if 
we could just use a home drinking filter with UV lighting for our water supply. 
Please let me know any thoughts or concerns you might have with this idea

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Distilled Water vs DI water for IHC and H staining

2016-07-28 Thread Whitaker, Bonnie via Histonet 
Hi Charles,

You will need to give it a try, in order to know for sure.

Water quality is EXTREMELY variable, from community to community, and even the 
pipes, etc., within in your building can influence the water quality.

The chemicals used by the local water district can also vary, based upon the 
season, and your geographical location.

Good luck!

Bonnie



Sent with Good (www.good.com)
Bonnie

-Original Message-
From: Charles Riley via Histonet 
[histonet@lists.utsouthwestern.edu]
Sent: Thursday, July 28, 2016 07:54 AM Eastern Standard Time
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Distilled Water vs DI water for IHC and H staining


Does anyone know if there is a difference in staining results when using DI
water versus Distilled water? Our lab is trying to get away from the DI
system we have as it is becoming expensive to maintain and we were looking
to see if we could just use a home drinking filter with UV lighting for our
water supply. Please let me know any thoughts or concerns you might have
with this idea

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] Distilled Water vs DI water for IHC and H staining

2016-07-28 Thread Charles Riley via Histonet 
Does anyone know if there is a difference in staining results when using DI
water versus Distilled water? Our lab is trying to get away from the DI
system we have as it is becoming expensive to maintain and we were looking
to see if we could just use a home drinking filter with UV lighting for our
water supply. Please let me know any thoughts or concerns you might have
with this idea

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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